Estimation of planting date in paddy fields by time-series MODIS data for basin-scale rice production modeling

Paddy and Water Environment - Projection of future changes in agricultural water demand and supply through numerical model simulations can be one of the reference materials used for long-term rural...     

Characterisation of QoI‐resistant field isolates of Botrytis cinerea from citrus and strawberry

BACKGROUND: In 2004, field isolates of Botrytis cinerea Pers. ex Fr., resistant to strobilurin fungicides (QoIs), were first found in commercial citrus orchards in Wakayama Prefecture, Japan. Subsequently, QoI‐resistant isolates of this fungus were also detected in plastic strawberry greenhouses in Saga, Ibaraki and Chiba prefectures, Japan. Biological and molecular characterisation of resistant isolates was conducted in this study. RESULTS: QoI‐resistant isolates of B. cinerea grew well on PDA plates containing kresoxim‐methyl or azoxystrobin at 1 mg L^?1, supplemented with 1 mM of n‐propyl gallate, an inhibitor of alternative oxidase, whereas the growth of sensitive isolates was strongly suppressed. Results from this in vitro test were in good agreement with those of fungus inoculation tests in vivo. In resistant isolates, the mutation at amino acid position 143 of the cytochrome b gene, known to be the cause of high QoI resistance in various fungal pathogens, was found, but only occasionally. The heteroplasmy of cytochrome b gene was confirmed, and the wild‐type sequence often present in the majority of resistant isolates, indicating that the proportion of mutated cytochrome b gene was very low. CONCLUSION: The conventional RFLP and sequence analyses of PCR‐amplified cytochrome b gene are insufficient for molecular identification of QoI resistance in B. cinerea. Copyright © 2009 Society of Chemical Industry     

Anti-tumor promoters phenolics and triterpenoid from Hippophae rhamnoides

A 70% ethanol extract of the branches of Hippophae rhamnoides exhibited remarkable antitumor activity in an in vivo two-stage carcinogenesis test in mice using 7,12-dimethylbenz[a]anthracene as an initiator and 12-O-tetradecanoylphorbol-13-acetate (TPA) as a promoter. From the active fraction of the 70% ethanol extract, three phenolic compounds, (+)-catechin (1), (+)-gallocatechin (2), and (−)-epigallocatechin (3) and a tritepenoid, ursolic acid (4) were isolated and identified. These compounds were evaluated for their inhibitory effects on TPA-induced inflammation (1 µg/ear) in mice. Within the tested compounds, 3 and 4 showed marked anti-inflammatory effects, with a 50% inhibitory dose of 1.7 and 0.2 μmol/ear.     

夏季飞来昆虫对放牧牛摆尾行为的影响

本研究选择黑毛和牛种牛4头作为实验牛。从2000年8月至10月，在0.8ha的实验牧草地进行了实验。实验结果表明：飞来昆虫消化的季节，飞来昆虫对放牧牛摆尾行为的影响可能存在个体飞来昆虫不仅引起牛的体表护理行为，还可能导致心理上的应激。     

Magnetic resonance imaging of a canine eye with melanoma

An 8-year-old Beagle dog had exophthalmos of the left eye for the last two past months. On ophthalmoscopy, the intraocular lesion could not be evaluated due to the opacity of the cornea. Ultrasonography revealed that the eyeball was distorted in shape and shifted in position, however, the precise lesion could not be identified. On magnetic resonance (MR) imaging, the lesion was observed as hyperintense on T1-weighted and hypointense on T2-weighted images, similar to those reported in human melanoma. The lesion was histologically diagnosed to be malignant intraocular melanoma. Though this is only a case report, canine ocular melanoma may show the similar characteristic MR images as in human uveal malignant melanoma.     

An outbreak of systemic granulomatous disease in cows with high milk yields

Seven of 92 lactating Holstein cows on a dairy farm developed urticaria with alopecia and decreased milk production, and three of the seven died over the course of 7 to 18 days. Pathologic examination of the three cows, including the two dead and one euthanized cow, revealed that the skin, liver, spleen, kidneys, heart, salivary glands, pancreas, adrenal glands, mammary glands, lymph nodes, and trigeminal ganglia had lymphocytic to lymphogranulomatous inflammation. Inflammation predominated by lymphocytic infiltration was prominent in the heart, pancreas, mammary glands, adrenal gland, and trigeminal ganglia. Severe granulomatous inflammation with multinucleated giant cells was present in the spleen and kidneys. These lesions and their distributions were most similar to those seen in suspected cases of citrus pulp toxicosis and hairy vetch toxicosis. The outbreak of this disease resolved with the elimination of Citrus pulp from the feed. Immunohistochemical detection of lymphocytes and macrophage markers confirmed dramatic hyperplasia of CD3-positive T lymphocytes in these lesions. This strongly suggested that a type 4 hypersensitivity reaction played a role in the development of the lesions.     

Chloramphenicol treatment for rabbit syphilis

Penicillin, the recommended treatment for rabbit syphilis, sometimes induces adverse effects. The efficacy of oral chloramphenicol was evaluated in 39 cases of rabbit syphilis to establish a safe and efficient treatment for this disease in companion rabbits. All cases clinically improved and recovered promptly. Fourteen of 39 cases (35.9%) relapsed, but most remained chloramphenicol sensitive. Since safety take priority over efficacy in treating syphilis in companion rabbits, chloramphenicol should be chosen as a first-line agent, as a general rule. Three-week administration of chloramphenicol may be adequate at the initial onset of disease. When relapse occurs repeatedly or the rabbit owner cannot administer the medicine adequately, treatment with penicillin should be considered.     

Diseases and outcomes in rabbits with high BUN levels

One hundred and ninety cases of rabbits, seen at animal hospitals in Saitama and Tokyo, Japan from 1998 to 2001, with BUN values greater than 27 mg/dl were analyzed regarding their underlying and/or complicating diseases and outcomes. Gastrointestinal disorder (54 cases) was the most common disease, followed by overgrowth of molar teeth and then liver disturbance. The total mortality was 48.9% within three months, and cases showing complications such as liver disturbance or bacterial infection showed highest mortality. Cases with higher BUN values showed even higher mortality, although mortality varied depending on the complications. Therefore, the prognosis of rabbit cases with high BUN values should be evaluated based on findings from blood chemistry, together with the seriousness of the underlying and/or complicating disease.     

Rabbit syphilis diagnosed clinically in household rabbits

This paper deals with 16 cases presented from April to December 2001 and diagnosed clinically as rabbit syphilis, because they showed distinct lesions around the nose and/or mouth, responded to chemotherapy, and the "Rapid Plasma Reagin" test was positive. Twelve cases exhibited initial symptoms and four were relapses. Lesions around the genitalia and/or anus as well as the nose and/or mouth were seen in 8 cases, and 6 cases indicated sneezing. Fifteen cases were successfully treated with oral administration of chloramphenicol, and one was treated with long-acting penicillin by intramuscular injection. The mean age of onset was 8.8 months. As none of these cases had any mating history, the disease was likely to be maternally transmitted.