Effects on soils and leaching after application of dolomite to an acidified forested catchment in the Lake Gårdsjön watershed,south-west Sweden

The dry forest soils (80%) of a 3.3 ha catchment in Gårdsjön, were treated with 1.5 ton/ha dolomite in 1984. In half the catchment an additional dose of 4.5 ton/ha was added in 1986 (i.e. totaly 6 ton/ha). By 1991 the concentrations of exchangeable calcium and magnesium had increased by 450% to 800% in the humus layer and upper 0–5 cm of the mineral soils and by 500% and 300%, respectively, in the 5–10 cm and 10–20 cm soil depth in the 6 ton/ha part. By 1.5 ton, Ca and Mg increased in humus and 0–5 cm in the mineral soil. Potassium decreased in the humus and the mineral soil with both 1.5 ton and 6 ton per ha. The accumulated output of K by runoff was however only 1 kg during the 6 years until 1992, which indicates that the losses from the soil also may have resulted in increased uptake by the forest. Tree growth and nutrient content in current needles was, however, unchanged. Increased contents of Mg, Ca and Zn was observed in some species of ground vegetation. Nitrate and ammonium concentrations and fluxes in runoff were identical to the reference catchment, while inorganic Al and Mn decreased by 40–50% and H⁺ by 30%. The accumulated extra output by runoff of Mg was about 7% and of Ca 0.6% of the amounts applied by the two treatments in 1984 and 1986.     

Antioxidants, low molecular weight carbohydrates, and total antioxidant capacity in strawberries (Fragaria x ananassa): effects of cultivar, ripening, and storage

Four cultivars of strawberries (Senga Sengana, BFr77111, Elsanta, and Honeoye) were studied for their content of antioxidants, total antioxidant capacity, and low molecular weight carbohydrates in relation to harvest year, ripening stage, and cold storage. For ascorbic acid, chlorogenic acid, ellagic acid, and total antioxidative capacity, measured in both water-soluble and water-insoluble extracts, there was a 2-5-fold variation among cultivars. Unripe berries contained lower concentrations of chlorogenic acid and p-coumaric acid and also quercetin and kaempferol compared with riper berries. During cold storage for up to 3 days, relatively few changes in the concentration of the different antioxidants occurred. The concentrations of several investigated parameters were interrelated, for example, for ascorbic acid and water-soluble antioxidant capacity and for ellagic acid and water-insoluble antioxidant capacity. The dominating sugars in strawberries were fructose and glucose, but considerable amounts of sucrose were also present, and their contents varied among cultivars, giving a predicted glycemic index of approximately 81. Verbascose, raffinose, and stachyose were found in only minor amounts. The study shows that the concentration of a number of bioactive compounds in strawberries varied according to cultivar, ripening stage, and storage. This information should make it possible to select strawberries with an optimal content of bioactive compounds.     

四川主要农用土壤中丛枝菌根真菌数量和种类的初步研究

用形态学分类法测土壤样品中丛枝菌根真菌的数量和种类分布特点,结果表明,丛枝菌根真菌广泛分布于四川农业土壤中,但其数量和种类较少,种群结构单一。共计分离鉴定出3个属18种丛枝菌根真菌,球囊霉属(Glomus)13种,无梗囊霉属(Acaulospora)2种,盾巨孢囊霉属(Scutellospora)3种,其中只有Gl.mosseae,Gl.cale-donium,Gl.constrictum的出现频率大于10%,是主要四川省农用土壤中的优势种。研究还发现,地域因素、土壤类型和土壤的理化性质以及人为因素对丛枝菌根真菌的数量和种类有显著影响(P<0.05)。     

Immunological characterization of the equine airway epithelium and of a primary equine airway epithelial cell culture model

Our understanding of innate immunity within the equine respiratory tract is limited despite growing evidence for its key role in both the immediate defense and the shaping of downstream adaptive immune responses to respiratory disease. As the first interface to undergo pathogen invasion, the respiratory epithelium is a key player in these early events and our goal was to examine the innate immune characteristics of equine respiratory epithelia and compare them to an in vitro equine respiratory epithelial cell model cultured at the air-fluid interface (AFI). Respiratory epithelial tissues, isolated epithelial cells, and four-week old cultured differentiated airway epithelial cells collected from five locations of the equine respiratory tract were examined for the expression of toll-like receptors (TLRs) and host defense peptides (HDPs) using conventional polymerase chain reaction (PCR). Cultured, differentiated, respiratory epithelial cells and freshly isolated respiratory epithelial cells were also examined for the expression of TLR3, TLR9 and major histocompatibility complex (MHC) class I and class II using fluorescence-activated cell sorting (FACS) analysis. In addition, cytokine and chemokine profiles from respiratory epithelial tissues, freshly isolated respiratory epithelial cells, and cultured, differentiated, epithelial cells from the upper respiratory tract were examined using real-time PCR. We found that respiratory epithelial tissues and isolated epithelial cells expressed TLRs 1-4 and 6-10 as well as HDPs, MxA, 2'5' OAS, β-defensin-1, and lactoferrin. In contrast, epithelial cells cultured at the AFI expressed TLRs 1-4 and 6 and 7 as well as MxA, 2'5' OAS, β-defensin-1, but had lost expression of TLRs 8-10 and lactoferrin. In addition, MHC-I and MHC-II surface expression decreased in epithelial cells cultured at the AFI compared to isolated epithelial cells whereas TLR3 and TLR9 were expressed at similar levels. Lastly, we found that equine respiratory epithelial cells express an array of pro-inflammatory, antiviral and regulatory cytokines and that after four weeks of in vitro growth conditions, equine respiratory epithelial cells cultured at the AFI retained expression of GM-CSF, IL-10, IL-8, TGF-β, TNF-α, and IL-6. In summary, we describe the development of an in vitro equine respiratory epithelial cell culture model that is morphologically similar to the equine airway epithelium and retains several key immunological properties. In the future this model will be a used to study equine respiratory viral pathogenesis and cell-to-cell interactions.     

Comparative survival and growth performance of European lobster Homarus gammarus post‐larva reared on novel feeds

One approach to ongrow juvenile European lobster, Homarus gammarus, is to utilize land based rearing systems, incorporating automated feeding, individual culture and provision of stable pelleted feeds, preferably using sustainable ingredients. We initiated three feeding experiments to investigate the general suitability of ingredients produced from seafood by‐products as novel feeds for H. gammarus, in terms of promoting survival, development and growth of post‐larval lobsters from post‐larvae (PL) stage IV to the first juvenile stage (stage V). The first experiment was designed to screen an array of candidate, locally produced, novel protein sources on growth performance parameters. This initial experiment revealed that PL reared on a raw (i.e. wet, unprocessed shrimp) feed used as a reference showed superior performance to those reared on experimental feeds containing fishmeal, herring protein isolate or mussel meal; however, a novel type of shrimp meal, produced by flocculation from waste water, promoted the best PL performance of any experimental feed. A second experiment was designed to test the effect of drying method and to optimize the form of a wet shrimp reference feed used by lobster hatcheries. This showed that the performance of PL reared on experimental freeze‐dried shrimp feed was not significantly different to those reared on the wet, unprocessed shrimp used as a reference feed. However, lobsters offered experimental oven‐dried shrimp feed (with or without an immune supplement) resulted in significantly lower survival or growth performance. A third and final experiment was designed in an attempt to improve a candidate herring‐based protein source, by supplementing with nutrients found in shrimp. However, the results showed that PL reared on the wet reference shrimp feed still showed superior growth and survival than those reared on a herring feed alone, or supplemented with additives found in shrimp meal (either glucosamine, astaxanthin or both supplements combined). The high survival and growth, low incidence of moulting problems and high availability of waste shrimp material, suggest that non‐heat‐treated shrimp products are a promising feed ingredient for post‐larval European lobsters.     

Health of farmed fish: its relation to fish welfare and its utility as welfare indicator

This brief review focuses on health and biological function as cornerstones of fish welfare. From the function-based point of view, good welfare is reflected in the ability of the animal to cope with infectious and non-infectious stressors, thereby maintaining homeostasis and good health, whereas stressful husbandry conditions and protracted suffering will lead to the loss of the coping ability and, thus, to impaired health. In the first part of the review, the physiological processes through which stressful husbandry conditions modulate health of farmed fish are examined. If fish are subjected to unfavourable husbandry conditions, the resulting disruption of internal homeostasis necessitates energy-demanding physiological adjustments (allostasis/acclimation). The ensuing energy drain leads to trade-offs with other energy-demanding processes such as the functioning of the primary epithelial barriers (gut, skin, gills) and the immune system. Understanding of the relation between husbandry conditions, allostatic responses and fish health provides the basis for the second theme developed in this review, the potential use of biological function and health parameters as operational welfare indicators (OWIs). Advantages of function- and health-related parameters are that they are relatively straightforward to recognize and to measure and are routinely monitored in most aquaculture units, thereby providing feasible tools to assess fish welfare under practical farming conditions. As the efforts to improve fish welfare and environmental sustainability lead to increasingly diverse solutions, in particular integrated production, it is imperative that we have objective OWIs to compare with other production forms, such as high-density aquaculture. However, to receive the necessary acceptance for legislation, more robust scientific backing of the health- and function-related OWIs is urgently needed.     

Dendroclimatic signals of pedunculate oak (Quercus robur L.) in Estonia

This study investigates the climate impact on the radial increment of pedunculate oak (Quercus robur L.) in Estonia at the species’ northern distribution limit. Tree-ring width series of 162 living oaks were compiled into three regional chronologies—western (1646–2008), northeastern (1736–2011), and southeastern Estonia (1912–2011). Although these regional growth patterns are similar to each other and even to the growth patterns in adjacent regions, spatial differences in growth responses to climate were established. Thus, oaks growing on shallow soil in western Estonia are positively influenced by summer (June–August) precipitation, and oaks on the deeper soil in northeastern Estonia are favoured by June temperature, while oaks in the southeastern part of the country depend on both July precipitation and temperature. These relationships are pronounced especially in pointer years. However, due to the impact of regional weather fluctuations on tree growth, there is a lack of correspondence between the local and the pan-European pointer years. In addition, our research presents the first tree-ring-based palaeoclimatic reconstruction for the country. Although the created model has relatively low predictive skill describing less than a quarter of the variance in actual summer precipitation in western Estonia, it has passable capacity of detecting past rainfall extremes.     

The value of a craft brewery: On the relationship between craft breweries and property values

Recent literature and public discourse emphasize the role of craft breweries in the revitalization process of economically distressed neighborhoods across the U.S. With this, many are reporting rising property values in areas surrounding craft breweries. This research examines the impacts of craft breweries on both residential and commercial property values in a case study of Charlotte, North Carolina. Estimating a series of hedonic models which incorporate a difference‐in‐difference estimator, we find that craft breweries have a positive impact on residential property values. While many areas in close proximity to a craft brewery appear to have been associated with relatively higher price premiums even before the opening of the brewery, breweries tend to add to this premium, especially in center‐city neighborhoods. We find no significant impacts on commercial property values. These results are informative to policy makers considering revising zoning laws and other regulations in efforts to promote the growth of craft breweries and spur economic development in their local economies.