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221.
Plasma cholinesterase (pChE) levels and erythrocyte acetylcholinesterase (eAChE) levels were studied in 6 cows before, during and after parturition (Group I), their calves (Group II), 38 cows suffering from parturient paresis (Group III) and 14 newly delivered non-paretic cows (Group IV).The mean of the pChE level in Group I was 1.5 μkat/1 ± 0.20 before parturition and decreased significantly (P ≦ 0.05) to 1.2 ukat/1 ± 0.16 after parturition. The eAChE level was before parturition ≅ 140 ukat/1 and decreased to ≅ 130 μkat/1 4–5 weeks after parturition.At birth the pChE level was 12.8 ukat/1 ± 5.9 in Group II. After 4 weeks the level had decreased to 2.3 ukat/1 ±0.3. In the bull calves the pChE level started to increase when they were 6 weeks old and reached a level of 5.7 μkat/1 ± 0.6 before slaughter at 6 months of age. The heifers did not show this increase. They had a level of around 2 μkat/1 throughout the investigation. The eAChE level at birth was 119 μkat/1 and increased slowly to a level of 145 μkat/1 at 6 months. No differences between the sexes were found.The cows suffering from parturient paresis had a pChE level of 1.80 μkat/1 ± 0.30 before treatment with calcium (Ca). The level decreased significantly (P ≦ 0.001) after Ca-infusion to a level of 1.67 ukat/1 ±0.29. Group IV had a pChE level of 1.65 μkat/1 ± 0.42 at parturition. Two to 4 months later the cows that had recovered from milk fever had a level of 1.61 μkat/1 ± 0.31 and the control cows 1.66 ukat/1 ± 0.48. No differences between the groups were found for the eAChE level.The findings show that parturition influences the pChE level in cows and that sex influences the pChE level in calves between 6 weeks to at least 6 months of age. Furthermore the elevated pChE level found in the cows suffering from parturient paresis before Ca infusion may be a further sign of a disturbance in the cholinergic system with a special preference to the neuromuscular junctions. 相似文献
222.
Feline insular amyloid: immunohistochemical and immunochemical evidence that the amyloid is insulin-related 总被引:3,自引:0,他引:3
K H Johnson P Westermark G Nilsson K Sletten T D O'Brien D W Hayden 《Veterinary pathology》1985,22(5):463-468
Utilizing islet amyloid-laden pancreatic tissues from six diabetic cats, we demonstrated substantial immunoreactivity (peroxidase-antiperoxidase technique) of the islet amyloid with antiserum to a B chain-rich insulin fraction, but no reactivity with antisera to insulin, glucagon, or somatostatin. Islet amyloid was purified from two cats and a protein unique to the diabetic and islet amyloid-laden cats was separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Immunoreactivity of this protein with antiserum to the B chain-rich insulin fraction was also shown by immunoblotting. Attempts to obtain the amino acid composition of the purified unique protein (represented by a single 25,000 dalton band on gel electrophoresis) were not successful because the amount of protein was too small. These results provide important additional evidence that an insulin-related protein is involved in the formation of islet amyloid. Our study also shows that the diabetic cat provides several advantages for the continued study of the etiopathogenic relationship of islet amyloid and diabetes mellitus. 相似文献
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Zoric M Sjölund M Persson M Nilsson E Lundeheim N Wallgren P 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2004,51(6):278-284
A group of 175 newborn piglets were monitored with respect to development of abrasions and lameness. Lameness was diagnosed in 10.9% of the piglets. About every second litter was affected and around 75% of these diagnoses took place during the first 3 weeks of life. Skin lesions were present already on day 3. They increased in magnitude until day 10 and thereafter declined. They were generally bilateral and most commonly observed as abrasions over the carpal joints. Hocks, face and tails were affected in a similar way, but at lower magnitudes. Sole bruising was observed in 87% of the piglets on the third day of life, and moderate to severe lesions dominated until day 10. Thereafter the incidence decreased, indicating healing with time. Still 39% of the piglets were affected at day 17. There was a significant positive correlation between skin lesions of carpus and hock within all examination days in selected piglets with known identity (n = 48). Between day 10 and 17 significant positive correlations were found within all examination sites with exception of abdomen and teats. The offspring of sows treated against mastitis expressed more abrasions then piglets delivered by healthy sows and the mortality during the first 17 days postpartum was significantly higher among piglets delivered by sows treated for mastitis. The level of serum antibodies to Streptococcus equisimilis in eight dams decreased during the last month of gestation and a declining maternal immunity to S. equisimilis was demonstrated in all piglets (n = 47) during the first 5 weeks of life. During the first 2 weeks of life somewhat lower median levels of serum antibodies were recorded among the piglets that were treated against arthritis (n = 8). 相似文献
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Laura Johnson Kristina Simone John Cullen Ashley Talley Eli B. Cohen 《Veterinary radiology & ultrasound》2019,60(6):E62-E65
A 10‐week‐old spayed female German Shepherd Dog was presented for acute vomiting and diarrhea. There was no reported foreign body or toxin ingestion. Radiographs showed a severely (~11 × 7 cm), focally distended right abdominal intestinal segment containing gas and soft tissue material. Other small intestinal segments were segmentally gas distended. Celiotomy identified a ~9 cm focally dilated segment at the jejunoileal junction with no aborad luminal obstruction. Resection and anastomosis of the dilation was performed. Histopathology showed mild mucosal inflammation, but otherwise normal wall layering and autonomic ganglia. Radiographic and histopathologic findings were consistent with congenital segmental dilation of the intestine. 相似文献
228.
Kathryn Y. Bray Karen R. Muñana Kristina Meichner Laura A. White Gabriela Seiler 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2016,45(4):698-702
A 12‐year‐old cat was presented for evaluation of progressive tetraparesis. Magnetic resonance imaging of the cervical spine demonstrated T2‐hyperintensity, and contrast enhancement within the C4–C7 spinal cord, with marked meningeal contrast enhancement and segmental nerve root thickening. Lumbar cerebrospinal fluid contained 407 total nucleated cells/μL, with 99% eosinophils. The cat transiently improved with prednisolone, clindamycin, and ivermectin therapy, but subsequently worsened and was euthanized. Necropsy revealed an asymmetric infiltration predominantly of the white matter, meninges, and nerve roots of the C4–C6 spinal cord segments by an unencapsulated, poorly demarcated neoplasm composed of atypical lymphocytes admixed with eosinophils, causing perivascular hemorrhage and lytic necrosis. The neoplastic cells were immunoreactive for CD3, ultimately confirming T‐cell lymphoma. 相似文献
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Miller KM Li S Kaukinen KH Ginther N Hammill E Curtis JM Patterson DA Sierocinski T Donnison L Pavlidis P Hinch SG Hruska KA Cooke SJ English KK Farrell AP 《Science (New York, N.Y.)》2011,331(6014):214-217
Long-term population viability of Fraser River sockeye salmon (Oncorhynchus nerka) is threatened by unusually high levels of mortality as they swim to their spawning areas before they spawn. Functional genomic studies on biopsied gill tissue from tagged wild adults that were tracked through ocean and river environments revealed physiological profiles predictive of successful migration and spawning. We identified a common genomic profile that was correlated with survival in each study. In ocean-tagged fish, a mortality-related genomic signature was associated with a 13.5-fold greater chance of dying en route. In river-tagged fish, the same genomic signature was associated with a 50% increase in mortality before reaching the spawning grounds in one of three stocks tested. At the spawning grounds, the same signature was associated with 3.7-fold greater odds of dying without spawning. Functional analysis raises the possibility that the mortality-related signature reflects a viral infection. 相似文献