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81.
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Voigt J Gaafar K Kanitz W Precht D Becker F Schneider F Spitschak M Schönhusen U Junghans P Aschenbach JR Gäbel G 《DTW. Deutsche tier?rztliche Wochenschrift》2005,112(11):423-425
The fate of carbon from long-chain fatty acids and glucose in dairy cows which were fed with protected fat was studied using stable isotope technique. The experiment was carried out on two groups of dairy cows (n=16 in each group) during the first 15 weeks of the lactation period. The cows were fed isoenergetic and isoproteinogenous diets based on corn silage. About 1.8 kg of tapioca starch in the diet of the starch group was substituted by about 0.7 kg of rumen protected fat (Ca salts of palm oil and soybean oil) in the diet of the fat group. The carbon atoms of dietary fat were naturally depleted in 13C as compared to carbon atoms of starch. Daily milk performance and lactose output were significantly (P < 0.05) higher among the cows fed with fat diet. In comparison to the starch group, the enrichment of milk fat with 13C was significantly lower, while that of breath CO2 was significantly higher in the fat group (P < 0.05). This means the fatty acids were incorporated into milk fat in preference to metabolic oxidation. Further studies showed that blood glucose is oxidized to a lower extent and is used for the synthesis of lactose to a higher proportion if the cows were fed with the fat diet. The glucose entry rate into the body glucose pool was not different between the diets. In conclusion, the dietary fatty acids perform a glucose sparing effect and improve the glucose supply for the mammary gland. 相似文献
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Molecular wheat breeding by direct gene transfer 总被引:7,自引:0,他引:7
A method for efficient genetic transformation of wheat has been developed using immature embryos as targets for microprojectile-mediated
gene transfer and a helium driven particle delivery system. Screening and selection of transgenic cells, somatic embryos and
regenerated plants are performed with the gus-gene and the phosphinothricin acetyl transferase (bar) gene coding for Basta-resistance
as the selectable marker. On average, one fertile transgenic plant can be obtained from about 100 microprojectile treated,
immature embryos. The number of integrated copies of the transferred gene ranges from 1 up to about 10. Stable integrated
genes are inherited in most of the transgenic lines in a normal mendelian fashion segregating 3:1 in the F2. Homozygous, as well as heterozygous, lines have been followed and analysed genetically at the molecular level and up to
F5. Apart from normal stable gene expression, examples have also been found which showed a loss of gene activity or unexpected
segregation pattern. For applied aspects, different genes are transferred aiming for improved disease resistance, modification
of quality, or other characteristics. First results from these transgenic lines are reported, and problems still existing
with the production of stable transgenic wheat lines are discussed.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
88.
E. Umapathy K. H. Erlwanger H. P. S. Makkar K. Becker S. G. Pierzynowski 《Journal of animal physiology and animal nutrition》1999,82(2-3):57-65
Introduction Cowpea (Vigna unguiculata) is a valuable protein source that can contribute towards overcoming a predicted protein deficit equivalent to more than 1 000 000 t oilcake in Southern Africa by the year 2000 (N ell et al. 1992). However, like most other legumes, cowpeas contain antinutritional factors (ANFs) (M akinde et al. 1996). It has also been shown that feeding raw cowpea causes reduced growth and histomorphometric changes in the various segments of the intestine (M akinde et al. 1997) attributable to ANFs. The major ANFs in the cowpea are trypsin inhibitors, tannins and phytates (A letor and A ladetimi 1989). Although the protease inhibitors are found in most legumes, only soybean inhibitors have been thoroughly investigated (L iener and K akade 1980), and trypsin inhibitors in other legumes have received little attention. 相似文献
89.
甘蓝型油菜(Brassica napus)无花瓣突变体“ Ap-Tengbe ”花瓣性状的遗传规律 总被引:2,自引:0,他引:2
本项研究旨在探明甘蓝型油菜突变体Ap-Tengbe无花瓣性状的遗传规律,‘Ap-Tengbe’和德国栽培品种‘Falcon’之间通过正反交产生两个方向的F1,BC1-1(F1和‘Ap-Tengebe’之间),BC1-2(F1和‘Falcon’之间)以及F2各遗传群体,根据田间各遗传群体PDgr(花瓣度)的观察和统计,作者提出‘Ap-Tenge’突变体无花瓣性状的遗传由细胞质和两对核隐性基因共同控制 相似文献
90.
Rapid salinity changes affect the survival and physiology of a penaeid prawn: Implications of flood events on recruitment to the fishery
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K. J. Tyler A. Becker N. A. Moltschaniwskyj M. D. Taylor 《Fisheries Management and Ecology》2017,24(6):478-487
Estuaries act as nurseries for many penaeid prawns, but these habitats are highly susceptible to salinity decline through flooding. The rate of salinity decline and duration of exposure to non‐optimal salinity may affect survival and subsequent recruitment of prawns to the fishery. This study aimed to determine the effect of salinity fluctuations observed in local estuaries during flood events using a novel dilution approach. Mortality of juvenile Melicertus plebejus (Hess) was assessed after 24 hr exposure to 24 rates of salinity decline ranging from 0.01% to 20% per hr. After the salinity decline, prawns were held at the final salinities for five days before again assessing mortality as well as aerobic metabolic rate and prawn water content. Salinity decline from 36 to ~0.8 led to 50% mortality, but continued exposure to low salinity for five days increased mortality at this salinity to 99% and shifted the 50% mortality point to salinity ~5. Aerobic metabolic rate and water content data suggested the cause of mortality due to exposure to salinities < 5 was osmoregulatory failure. Rapid salinity declines over 24 hr and sustained low salinity due to flooding could compromise the survival of juvenile prawns, potentially reducing recruitment to the fishery. 相似文献