Community forestry for revitalising rural ecosystems: A case study

A case study focusing on the socio-economic and ecological aspects of a forest-based village, Debrasai, in the Singhbhum District of Bihar, India was undertaken in 1980 to evolve village-specific community forestry package programmes that could bring stability to the village both economically as well as ecologically. The village extending over 365.07 ha is inhabited by 361 people comprising 195 males and 166 females in 81 households. The villagers are by and large dependent on agriculture and forestry for their livelihood. The largest proportion of the land in the village is under agriculture whereas forests, in a highly degraded state, occupy only 5.7% of the total area. The villagers exploit the protected forests in their vicinity removing nearly 350.9 mt of fuelwood, 22.8 mt of small timber and 393.4 mt of fodder annually.The village has a manpower potential of 40 035 man days a year. Agriculture is the main source of employment at the moment absorbing 14 266 man days. The non-agricultural sector provides employment to an extent of 4433 man days. Fresh employment opportunities are needed to absorb nearly 53% of man days of the village at present unused. Three land-resource, skill-based package programmes, a tasar silk culture, an energy plantation, and dairying have been suggested to generate employment opportunities and to meet the basic needs of the villagers. About 115 ha of the hitherto neglected land resource has been located in the village to sustain such community forestry (CF) programmes. Tasar culture suggested on 35 ha will alone generate employment to an extent of 18 690 man days from the fourth year of tasar silk plantation. A plantation, to provide energy and fodder, on 25 and 30 ha respectively is expected to yield fuelwood, timber and fodder to more than satisfy village needs. A dairy has been recommended as it will generate income, provide nutrition and promote gobar gas production — an alternative energy source to fuelwood. The cost—benefit analysis of these projects indicates their economic feasibility with the cost likely to be recovered in 4.5 years. Debrasai being an agricultural village, a land-inequity compensatory scheme has been suggested for CF project net benefit sharing, ensuring reasonable benefit flows to the landless and marginal farmers.     

Development of Eco-friendly and Non-hazardous Weed Control Technology for Lowland Rice by Smothering through LDPE Film

Intensive use of chemical herbicides and pesticides has raised serious concern about their effect on non-target organisms, plants, human bodies and the environment. Hence there is a continuous search for benign, harmless, non-hazardous and eco-friendly technology to control the growth of weeds which compete with crops for water, nutrients, light and space; thus reducing crop yield significantly. An attempt was made to control the weeds through smothering. This controls the weeds by cutting off light from the photosynthetic portion of weed plants by using black polyethylene sheets (LPDE film) which inhibit the top growth. Experiments to control the weeds in a lowland rice field through smothering were conducted in the high rainfall coastal region of eastern India wth eight treatments including smothering and conventional methods like hand/mechanical weeding and herbicides, etc. The treatment of smothering for one week + puddling + transplanting proved to be better than other conventional methods and may replace herbicide and hand/mechanical weeding totally with a better rice yield.     

Pathotyping and antimicrobial susceptibility testing of Escherichia coli isolates from neonatal calves

Neonatal calf mortality is a major concern to livestock sector worldwide. Neonatal calf diarrhoea (NCD), an acute severe condition causes morbidity and mortality in calves. Amongst various pathogens involved in NCD, E. coli is considered as one of the major causes. The study was targeted to characterize E. coli isolates from neonatal calves for diarrhoeagenic Escherichia coli (DEC) types (pathotyping), antimicrobial resistance (AMR) profiling and to correlate with epidemiological parameters. From neonates, a total of 113 faecal samples were collected, out of that 308, lactose fermenting colonies were confirmed as E. coli. Pathotypable isolates (12.3%) were represented by STEC (6.1%), EPEC (2.9%), ETEC (1.9%), EAEC (0.9%) and EHEC (0.3%). Occurrence of STEC was more in non-diarrhoeic calves, whereas ETEC was observed more in diarrhoeic calves. EPEC occurrence was observed in both diarrhoeic and non-diarrhoeic calves. Fishers extract test showed no significant association for occurrence of DEC types to type of dairies, health status, species, breed, age and sex of neonatal calves. Two hundred and eighty isolates were tested for antimicrobial susceptibility. The isolates showed maximum resistance towards ampicillin (55.4%) followed by tetracycline (54.3%), while minimum resistance was observed towards meropenem (2.5%). Multidrug resistant E. coli isolates were found to be 139 (49.6%), and Extended-spectrum beta-lactamase (ESBL) producers were 120 (42.9%). DEC pathotypes like STEC, ETEC, EHEC and EAEC that are also multidrug resistant present in neonatal calves have zoonotic potential and hence are of public health significance.

     

Rate of stomatal opening, shoot hydraulic conductance and photosynthetic characteristics in relation to leaf abscisic acid concentration in six temperate deciduous trees

Correlations between leaf abscisic acid concentration ([ABA]), stomatal conductance (gs), rate of stomatal opening in response to an increase in leaf water potential (si), shoot hydraulic conductance (L) and photosynthetic characteristics were examined in saplings of six temperate deciduous tree species: Acer platanoides L., Padus avium Mill., Populus tremula L., Quercus robur L., Salix caprea L. and Tilia cordata Mill. Species-specific values of foliar [ABA] were negatively related to the mean values of gs, si, L and light- and CO2- saturated net photosynthesis (P(max)), thus providing strong correlative evidence of a scaling of foliar gas exchange and hydraulic characteristics with leaf endogenous [ABA]. In addition, we suggest that mean gs, si, L and Pmax for mature leaves may partly be determined by the species-specific [ABA] during leaf growth. The most drought-intolerant species had the lowest [ABA] and the highest gs, suggesting that interspecific differences in [ABA] may be linked to differences in species-specific water-use efficiency. Application of high concentrations of exogenous ABA led to large decreases in gs, si and P(max), further underscoring the direct role of ABA in regulating stomatal opening and photosynthetic rate. Exogenous ABA also decreased L, but the decreases were considerably smaller than the decreases in gs, si and Pmax. Thus, exogenous ABA predominantly affected the stomata directly, but modification of L by ABA may also be an important mechanism of ABA action. We conclude that interspecific variability in endogenous [ABA] during foliage growth and in mature leaves provides an important factor explaining observed differences in L, gs, si and Pmax among temperate deciduous tree species.     

Induction of immune tolerance to caseins and whey proteins by oral intubation in mouse allergy model

This study was designed to evaluate the effect of oral tolerance of caseins (CSN) and whey proteins (WP) in alleviating the allergic response to cow's milk proteins in Swiss albino mice raised on a milk protein–free diet. Oral tolerance was induced by feeding mice with 20 mg of CSN or WP once in a day for 4 days consecutively before immunization with respective protein by intraperitoneal (i.p.) injections (20 μg 200 per μl of PBS) using 2% of alum Al(OH)₃ as adjuvant. Three weeks later, oral tolerance induction was analysed in humoral and cellular compartments of CSN‐ and WP‐fed versus saline‐fed control mice groups by measuring seric and intestinal antibody responses, mRNA abundance in splenic tissue and cytokine secretion patterns. The specific serum immunoglobulin‐E (IgE) levels were significantly suppressed (p < 0.05), while sIgA was enhanced in these groups when compared with their respective saline‐fed mice. Moreover, the mRNA levels of interferon‐γ (IFN‐γ) and interleukin‐4 (IL‐4) in both CSN‐ and WP‐tolerized mice were found to be significantly decreased, while the abundance of interleukin‐10 (IL‐10) and transforming growth factor‐β (TGF‐β) was increased significantly, as compared to respective control groups. Finally, cytokine profiles indicated a reciprocal decrease in IL‐4 and IFN‐γ versus an increase in IL‐10 secretions in supernatants of cultured splenocytes of tolerized mice. Taken together, these results clearly showed that oral administration of cows' milk caseins and whey proteins can induce significant hyposensitization in mice, with the participation of suppressor cytokines.     

First record of the entomopathogenic fungus <Emphasis Type="Italic">Entomophaga aulicae</Emphasis> on the Bihar hairy caterpillar <Emphasis Type="Italic">Spilarctia obliqua</Emphasis> in Manipur,India

Entomophaga aulicae (Hoffman in Bail) Batko (Zygomycotina: Entomophthorales) was found to cause epizootics in Spilarctia obliqua (Walker) (Arctiidae: Lepidoptera), a polyphagous pest in Manipur, India. Infected caterpillars climbed to elevated positions on plants where they hung upside down, attached by their abdominal prolegs. Infection levels reached up to 57%. The conidiophores are simple, conidia are ovoid to pyriform in shape, and the nuclei stained readily in aceto-orcein. Secondary, tertiary conidia and resting spores were also observed. No rhizoids and cystidia were found. This is the first report of E. aulicae infecting S. obliqua in Manipur.