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31.
Grassly NC Fraser C Wenger J Deshpande JM Sutter RW Heymann DL Aylward RB 《Science (New York, N.Y.)》2006,314(5802):1150-1153
The feasibility of global polio eradication is being questioned as a result of continued transmission in a few localities that act as sources for outbreaks elsewhere. Perhaps the greatest challenge is in India, where transmission has persisted in Uttar Pradesh and Bihar despite high coverage with multiple doses of vaccine. We estimate key parameters governing the seasonal epidemics in these areas and show that high population density and poor sanitation cause persistence by not only facilitating transmission of poliovirus but also severely compromising the efficacy of the trivalent vaccine. We analyze strategies to counteract this and show that switching to monovalent vaccine may finally interrupt virus transmission. 相似文献
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Le Moigne V Cariolet R Béven V Keranflec'h A Jestin A Dory D 《Research in veterinary science》2012,93(2):1032-1035
This study was performed to determine whether electroporation can be used to enhance the efficacy of a DNA vaccine against pseudorabies virus (PrV) in pigs. Immune responses to PrV were measured in pigs following a single intramuscular injection of plasmids encoding PrV glycoprotein B, with or without electroporation. Plasmid injection coupled with electroporation increased production of specific antibodies against PrV and peripheral blood mononuclear cells proliferated in response to stimulation with PrV glycoproteins. These results show that electroporation can improve the performance of a DNA vaccine against PrV in pigs. However, additional work is required to maximise the effectiveness of the vaccination protocol. 相似文献
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Björn Kemmann Thorsten Ruf Reinhard Well Christoph Emmerling Roland Fuß 《植物养料与土壤学杂志》2023,186(1):79-94
Background
The sustainability of bioenergy is strongly affected by direct field-derived greenhouse gas (GHG) emissions and indirect emissions form land-use change. Marginal land in low mountain ranges is suitable for feedstock production due to small impact on indirect land-use change. However, these sites are vulnerable to high N2O emissions because of their fine soil texture and hydrology.Aims
The perennial cup plant (Silphium perfoliatum L.) might outperform silage maize (Zea mays L.) on cold, wet low mountain ranges sites regarding yield and ecosystem services. The aim of this study was to assess whether the cultivation of cup plant also provides GHG mitigation potential compared to the cultivation of maize.Methods
A t-year field experiment was conducted in a low mountain range region in western Germany to compare area and yield-scaled GHG emissions from cup plant and maize fields. GHG emissions were quantified using the closed chamber method.Results
Cup plant fields emitted an average of 3.6 ± 4.3 kg N2O-N ha–1 year–1 (–85%) less than maize fields. This corresponded to 74.0 ± 94.1 g CO2-eq kWh–1 (–78%) less emissions per produced electrical power. However, cup plant had a significantly lower productivity per hectare (–34%) and per unit of applied nitrogen (–32%) than maize.Conclusion
Cup plant as a feedstock reduces direct field-derived GHG emissions compared to maize but, due to lower yields cup plant, likely increases emissions associated with land-use changes. Therefore, the increased sustainability of bioenergy from biogas by replacing maize with cup plant is heavily dependent on the performance of maize at these sites and on the ecosystem services of cup plant in addition to GHG savings. 相似文献36.
Faster residue decomposition of brittle stem rice mutant due to finer breakage during threshing 总被引:1,自引:0,他引:1
Dinah M.S. Cabiles Olivyn R. Angeles Sarah E. Johnson-Beebout Pearl B. Sanchez Roland J. Buresh 《Soil & Tillage Research》2008,98(2):211-216
In intensive tropical rice (Oryza sativa L.) cropping systems with short fallows, it would be advantageous that rice straw decompose fast enough to facilitate land preparation and planting of the subsequent crop. The straw of a brittle stem rice mutant of IR68 was tested for more rapid decomposition compared with non-brittle IR68 straw. The hypothesis was that the brittle mutant straw would break into smaller pieces during threshing, and that both the smaller piece size and the differences in biochemical straw composition would enable more rapid decomposition. Brittle straw broke into smaller pieces than non-brittle straw during a replicated trial of three threshing methods: hand threshing, pedal threshing, and axial-flow machine threshing. In a litter bag study to determine the effect of straw piece size on decomposition rate of each straw type over 10 weeks, smaller straw pieces decomposed faster than larger pieces as indicated by changes in amount of straw and its C/N ratio over time (P < 0.05), but there was no significant difference between straw types at the same size. It was concluded that the finer breakage of brittle straw during field operations is likely to be more important than the biochemical differences in overall residue decomposition rate. 相似文献
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Arends B Spee B Hoffmann G Jansen GE Slump E Auriemma E Ijzer J Hemrika W Romijn RA van der Heijden-Liefkens KH Sondermeijer PJ van den Ingh TS Penning LC Rothuizen J 《Veterinary journal (London, England : 1997)》2008,178(1):70-77
Hepatocyte growth factor (HGF) is crucial for the development and regeneration of the liver and offers a possible new therapeutic strategy for the treatment of canine liver disease. In this study, the in vitro and in vivo bioactivity of recombinant canine HGF (rcHGF) produced with a baculoviral expression system in insect cells was measured. In vitro rcHGF induced mitogenesis, motogenesis, and phosphorylated the HGF receptor c-MET and its downstream mediators PKB and ERK1/2 in two canine epithelial cell lines. After a partial hepatectomy (phx) in dogs, rcHGF increased phosphorylation of c-MET, PKB and ERK1/2. A moderate increase was seen with the cell cycle protein PCNA in rcHGF treated livers, but no HGF-induced increase in liver weight was seen 7 days after phx. Furthermore, rcHGF treated livers showed lower levels of the key mediator of apoptosis, caspase-3, at 7days after phx. It is concluded that rcHGF is a biologically active protein in vitro and in vivo and the baculoviral expression system supplies sufficient amounts of rcHGF for future clinical studies in dogs. 相似文献
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For a long time studies on precipitation and lake-water chemistry have focussed on the deposition of strong acids. Dust in snow and rain was analyzed much less intensively, but recent findings suggest that it may be the missing link which explains why many low-alkalinity lakes in the Alps and the Pyrenees did not become acidic and nutrient levels are seasonally high, whereas lakes in areas which are rarely influenced by dust depositions, for instance in Scandinavia, have acidified. Beside being a dominant factor for nutrient inputs to oceans, rain forests and remote lakes, dust can also play a major role in global warming and cooling, and it may significantly contribute to soil formation at mountain sites in the Mediterranean. I suggest that future changes in dust deposition and warming will be key factors for the development of alpine lakes. 相似文献