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灌浆结实期高温对水稻籽粒蔗糖及降解酶活性的影响 总被引:8,自引:0,他引:8
选用水稻品种越光和笹锦为材料,在开花后设置自然温度和高温两种处理,研究了不同温度处理下籽粒蔗糖、果糖和葡萄糖含量的动态变化以及蔗糖合酶、液泡型转化酶和细胞壁结合型转化酶活性的差异。高温处理下,蔗糖合酶活性值大于转化酶活性值,且与淀粉积累速率呈显著正相关,表明蔗糖合酶在蔗糖的分解和淀粉的合成过程中起着重要作用;高温下两品种籽粒的蔗糖含量明显增加,但分解的葡萄糖和果糖含量并未相应增加,表明高温有利于籽粒中蔗糖的积累,而不利于籽粒中蔗糖的分解。高温下蔗糖合酶、液泡型转化酶和细胞壁结合型转化酶活性明显下降,表明蔗糖分解速率的下降与蔗糖合酶和转化酶活性下降有关。 相似文献
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KENSUKE NAKAMURA NOBORU SASAKI MUNEYOSHI YOSHIKAWA HIROSHI OHTA SHIANG-JYI HWANG TAKAHIRO MIMURA MASAHIRO YAMASAKI MITSUYOSHI TAKIGUCHI 《Veterinary radiology & ultrasound》2009,50(1):104-108
Contrast-enhanced ultrasonography has an important role in the detection of tumors in humans. The second-generation contrast agent Sonazoid has the ability of real-time contrast imaging along with parenchymal imaging. The purposes of this study were to determine the effect and duration of Sonazoid on the changes in gray-scale enhancement of canine spleen and to establish an appropriate protocol for contrast-enhanced ultrasonography of canine spleen. Six healthy beagles were injected with an intravenous bolus of Sonazoid. In the spleen parenchyma, the enhancement was maintained up to 30 min after injection. Moreover, for 5–22 s after injection, gray-scale enhancement of splenic arteries afforded arterial imaging. Perfusion of the kidney may be investigated from 3.6 s to 3.5 min after injection of Sonazoid. These results suggest that Sonazoid is applicable to canine spleen parenchymal imaging and that the optimal time for the parenchymal imaging is 7–30 min after injection. The findings of this quantitative study should prove useful in the evaluation of diffuse or focal splenic and renal diseases in dogs. 相似文献
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Naoki SASAKI Nao TSUZUKI Michiaki YAMADA Takuto MINAMI Haruo YAMADA 《Journal of Equine Science》2009,20(1):7-10
This study aimed to establish a standard dose and sample collection time for
13C phenylalanine and 13C-Dipeptide breath test in horses. To
evaluate dose-dependent effects, healthy horses received 2.5 mg/kg, 5 mg/kg, and 10 mg/kg
13C phenylalanine dissolved in 1 ml/kg distilled water and 1.25 mg/kg, 2.5
mg/kg, and 5 mg/kg 13C dipeptide dissolved in 2 ml/ kg distilled water. Tmax
was observed during the sample collection time. For 13C phenylalanine, the
standard deviation of Cmax at 5 mg/kg was lower than that of 10 mg/kg. For 13C
dipeptide, the standard deviation of Tmax was the lowest at 5 mg/kg. This study revealed
that an optimal dose for breath tests with 13C phenylalanine and 13C
dipeptide may be 5 mg/kg in horses. 相似文献
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Kensuke HIROSE Mariko NAKAMURA Tatsuya TAKIZAWA Kazuo FUKAWA Tetsuya ITO Masayuki UEDA Takashi SASAKI Kazuaki TANAKA 《Animal Science Journal》2009,80(6):624-630
An insertion/deletion variant of a thymine base (T5 and T6) in exon 2 of porcine beta 3‐adrenergic receptor (ADRB3) gene has been described. In the current study, we made an association study between the ADRB3 polymorphisms and production traits in 735 Duroc pigs. The allele frequencies for the T5 and T6 alleles in our study population were 0.433 and 0.567, respectively. Any associations between ADRB3 genotype and average daily weight gain during test period, or backfat thickness and intramuscular fat content were not detected in either sex. However the size of the loin eye muscle area (EMA) was significantly associated with ADRB3 genotypes in gilts. T6‐homozygous gilts had a higher mean of EMA (40.6 ± 0.6 cm2) than T5‐homozygous (38.1 ± 0.4 cm2, P = 0.002) and heterozygous (38.8 ± 0.3 cm2, P = 0.034) gilts. This association was not detected in males. In addition, a multiple traits animal model best linear unbiased predictor (BLUP) analysis revealed that the T6‐homozygous genotype had positive effects on breeding value of EMA. Accordingly, we suggest that ADRB3 polymorphism has the potential to be an important genetic marker for prediction of EMA in Duroc pigs. 相似文献
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Kenichi OTAWA Masaaki SATO Takako SASAKI Hiraku SASAKI Jun NONAKA Kikuji ITO Toshio KUROKI Yutaka NAKAI 《Animal Science Journal》2004,75(3):261-269
The ecology of shiga‐toxigenic Escherichia coli (STEC) is important in the animal production environment. We investigated fecal shedding of STEC in one town in Miyagi, Japan by multiplex polymerase chain reaction (PCR) targeting shiga toxin gene 1 (stx1), gene 2 (stx2) and malB promoter gene, and analyzed the PCR products of stx1 or stx2 (54 samples) by direct sequencing. Three of 46 (6.5%) beef cattle in the University Farm of Tohoku University (Kawatabi Farm) and 11 of 70 (15.7%) calves in neighboring dairy farms carried STEC. Rate of detecting genes of stx1, stx2 and stx1+2 was 3.4% (4/116), 8.6% (10/116) and 0.9% (1/116), respectively. Serotyping indicated that STEC contaminated farms at different times or through different routes. Isolates harbored no mutation among stx1, but six (Kawatabi Farm) and 38 (neighboring farms) base substitutions among stx2, respectively. The diversity of substitutions of stx2 was observed among farms or even in a farm. Phylogenic analysis revealed that STEC detected in the area were classified into three clusters by the variety of stx2. Sequence analysis of stx2 will be one of the tools for clarifying the source of outbreaks and the route of contamination of STEC. 相似文献