Tree mapping using airborne,terrestrial and mobile laser scanning – A case study in a heterogeneous urban forest

We evaluated the accuracy and efficiency of airborne (ALS), terrestrial (TLS) and mobile laser-scanning (MLS) methods that can be utilized in urban tree mapping and monitoring. In the field, 438 urban trees located in park and forested environments were measured and mapped from our study area located in Seurasaari, Helsinki, Finland. A field reference was collected, using a tree map created manually from TLS data. The tree detection rate and location accuracy were evaluated, using automatic or semiautomatic ALS individual tree detection (ALS_ITDauto or ALS_ITDvisual) and manual or automatic measurements of TLS and MLS (TLS_auto, MLS_auto, MLS_manual, MLS_semi). Our results showed that the best methods for tree detection were TLS_auto and MLS_manual, which detected 73.29% and 79.22% of the reference trees, respectively. The location accuracies (RMSE) varied between 0.44 m and 1.57 m; the methods listed from the most accurate to most inaccurate were MLS_semi, TLS_auto, MLS_manual, MLS_auto, ALS_ITDauto and ALS_ITDvisual. We conclude that the accuracies of TLS and ALS were applicable for operational urban tree mapping in heterogeneous park forests. MLS_manual shows high potential but manual measurements are not feasible in operational tree mapping. Challenges that should be solved in further studies include ALS_ITDauto oversegmentation as well as MLS_auto processing methodologies and data collection for tree detection.     

Sexual Reproduction of European Aspen (Populus tremula L.) at Prescribed Burned Site: The Effects of Moisture Conditions

Aspen (Populus tremula) is capable of reproducing both sexually and asexually from root suckers, the latter being more common. Seedlings are rare even though potential seed production is enormous. The aim of this study was to find out how different moisture conditions affect emergence and survival of aspen seedlings on burned soil. We set up a sowing experiment on mineral soil and on humus. The sowing was done at 240 microsites (θ7 cm) in 15 blocks (1×1 m). Treatments were sowing time, watering and sowing shelter. A total of 1463 seedlings emerged on mineral soil and 26 on humus. Watering, covering, their interaction, block and sowing time had significant effects on the number of seedlings. The largest numbers of seedlings were recorded at microsites that were watered and covered. The survival of seedlings was 20% during the growing season and 45% over winter. We conclude that seedbed conditions and moisture are the most essential factors for sexual reproduction of aspen. Short-term weather conditions have an important effect on seedling emergence and are likely to greatly affect field studies.     

Deamidation of gluten proteins and peptides decreases the antibody affinity in gluten analysis assays

Wheat gluten is a widely used ingredient in the food industry due to its unique properties and relatively low price. Modification of wheat gluten makes it a versatile ingredient and, thus, increases its applicability in foods. Therefore, gluten proteins can be found in unexpected sources, and this makes the gluten-free diet challenging to follow. Deamidation is one way to modify protein structure. It increases solubility and surface activity of gluten improving its functionality, but consequently, also influencing the accuracy of quantification by immunoassays. In this study, the effect of deamidation on the antibody recognition with gluten analysis methods based on monoclonal R5, omega-gliadin or G12 antibodies was studied. Random deamidation decreased the intensities to 13–54% of the intensity obtained for the intact peptides. Deamidation representing the transglutaminase deamidation decreased the intensities to 4–8%. Deamidation of gluten proteins abolished the recognition by omega-gliadin and G12 antibodies and decreased the recognition of R5 by 600 times when analyzed by the sandwich method and 125 times by the competitive method. In conclusion, with all of the investigated gluten-specific antibodies, deamidation decreased the affinity of antibodies to gluten peptides and proteins, which needs to be considered when assays and regulations are developed for gluten-free products.     

Malt hydrolysates for gluten-free applications: Autolytic and proline endopeptidase assisted removal of prolamins from wheat,barley and rye

Cereal based products intended for gluten sensitive individuals, particularly to celiac disease patients, tend to have poor organoleptic qualities and they contain low levels of healthy whole grain compounds. Adding whole grain ingredients, such as malt hydrolysates, could compensate these defects provided that the ingredients are adequately free from toxic prolamin epitopes. Here we demonstrate that the level of toxic prolamin epitopes in the malt autolysates (wheat, barley, rye) were substantially lower than in the native malts but too high to allow “very low in gluten” labelling. To further eliminate the residual levels of toxic prolamin epitopes, a proline-specific endoprotease from Aspergillus niger was added to the malt autolysates. In the resulting malt hydrolysates (of wheat and rye but not barley), the prolamins were indeed greatly reduced and were below the very low gluten limit of 100 mg/kg. Malt hydrolysates with adequately low gluten levels may potentially be used as novel ingredients within gluten-free foods.     

Ice fishing reveals size structure but not abundance of Eurasian perch in small boreal lakes

Management of inland recreational fisheries would benefit from stock abundance and size structure data. Feasibly standardised angling methods such as ice fishing could produce representative catch-per-unit-effort (CPUE) information on the abundance of different-sized fish in small lakes. Here, we first used standard Nordic multimesh gillnets to obtain number-per-unit-effort (NPUE), biomass-per-unit-effort (BPUE) and size structure data on Eurasian perch (Perca fluviatilis) stocks in 11 small boreal lakes in summer. Second, the same lakes were ice-fished by voluntary anglers using a pre-defined, loosely standardised protocol to obtain angling-based NPUE, BPUE, and length frequency distributions. Effects of environmental variables such as water oxygen concentration and light penetration on angling catch rates were controlled statistically. Neither perch Nordic gillnet NPUE nor BPUE corresponded to ice-fishing CPUEs. However, the length distribution of the catch did not differ between methods. Our results imply that traditional ice fishing applying natural baits is relatively unselective for fish size and could produce valid length-based indicators for management purposes while angling CPUE was poorly related to Nordic gillnet CPUE.     

Microbiological Contamination of Reindeer Carcass During Slaughter

Microbiological counts for 10 different sampling sites of 28 reindeer carcasses were studied in 3 reindeer slaughterhouses in Finland. On each carcass the hindshank, round, abdomen, flank, brisket, foreleg, shoulder, neck, foreback and back were sampled immediately after slaughter, using a nondestructive swabbing method. The overall mean bacterial count for 10 sampling sites of reindeer carcasses was 1.51 ± 0.51 log₁₀ cfu/cm². Statistically significant differences were detected between sampling sites. The back part of the reindeer carcass, i.e. hindshank, round, back and foreback, seemed to be relatively clean. The most contaminated parts were the foreleg, brisket and abdomen (2.05–2.95 log₁₀ cfu/cm²); these could be used for monitoring the hygiene of the reindeer carcass after slaughter. Differences between the 3 slaughterhouses were detected for some sampling sites, which may be due to differences in slaughter techniques and hygiene.     

The interaction of cadmium and selenium in horse kidney cortex in relation to histopathological changes

The kidney cortex of 32 Finnish horses was analysed chemically for cadmium (Cd) and selenium (Se) content and by light microscopy for histopathological changes of the tissues. Cd concentrations in kidney cortex ranged from 6.9 to 91.6 mg/kg wet weight with an average of 31.9 mg/kg. Se concentrations ranged from 0.5 to 1.5 mg/kg with an average of 1.0 mg/kg. The age of the horses varied from 1.5 to 32 years; mean age was 16 years.Cd levels in kidney cortex seemed to increase linearly up to an age of about 16 years. In old (over 16 years) horses no such correlation could be found. Se concentrations were found to decrease in relation to age. The horses with higher than 1 mg/kg Se in kidney cortex had less PAS-positive casts than horses with low Se content. Among the total population of the horses no correlation could be demonstrated between Cd and Se concentrations in kidney cortex. However, the horses with more than 40 mg/kg Cd in their kidney cortex had a slightly positive correlation between Cd and Se concentrations.     

Estimating forest data for analyses of forest production and utilization possibilities at local level by means of multi-source National Forest Inventory

The sample plot data of National Forest Inventories (NFI) are widely used in the analysis of forest production and utilization possibilities to support national and regional forest policy. However, there is an increasing interest for similar impact and scenario analyses for strategic planning at the local level. As the fairly sparse network of field plots only provides calculations for large areas, satellite image data have been applied to produce forest information for smaller areas. The aim of this study was to test the feasibility of generating forest data for a Finnish forest analysis tool, the MELA system, by means of the Landsat satellite imagery and the NFI sample plot data. The study was part of the preparation of a local forestry programme, where a strategic scenario analysis for the forest area of two villages (ca 8000 ha) was carried out. Management units that approximate forest stands were delineated by image segmentation. Stand volume and other parameters for each forest segment were estimated from weighted means of the NFI sample plots, where the individual sample plot weights were estimated by the k nearest neighbour (kNN) method. Two different spectral features were tested: single pixel values and average pixel values within a segment. The estimated forest data were compared with the forest data based on independent stand-level field assessments in two subareas, a national park and an area of forest managed for timber production.In the national park, the estimated mean volume of the growing stock from both spectral feature sets (about 160 m³ ha⁻¹) was clearly lower than that obtained from stand-level field assessment (186 m³ ha⁻¹). Using average pixel values within a segment resulted in a higher proportion of pine and a lower proportion of spruce volume than using single pixel values. It also resulted in an estimated felling potential nearly 10% higher over the first 10-year period in the scenario analysis of the area dedicated to timber production. However, the maximum long-term sustainable removal was at the same level (about 30,000 m³ year⁻¹) for both feature sets over the simulated 30-year period. The resulting annual felling area in the first 10-year period was 12% lower when the segment averages were applied, but the difference subsequently levelled off. The kNN approach in estimating initial forest data for scenario analyses at the local level was found promising.     

Effects of varying thinning regimes on carbon uptake,total stem wood growth,and timber production in Norway spruce (<Emphasis Type="BoldItalic">Picea abies)</Emphasis> stands in southern Finland under the changing climate

• Introduction  

Based on previous studies, it is assumed that the growth of Norway spruce (Picea abies) in southern Finland (61°N) may decrease under the changing climate due to the increasing soil water deficit, without management.