Effects of butyrate supplementation in antibiotic‐free milk replacer and starter on growth performance in suckling calves

The aim of this study was to evaluate butyrate supplementation of antibiotic‐free milk replacer and starter on growth performance in male Holstein calves. Twenty‐nine calves were divided into two groups. Group C (n = 13) was fed antibiotic‐free milk replacer without supplementation, and Group B (n = 16) was fed antibiotic‐free milk replacer supplemented with butyrate (1.6 % DM of Gustor BP70^®). Starter in Group B contained 0.3 % DM of Gustor BP70^®. The intake of milk replacer was lower in group B than in C (p = 0.07 for the treatment x week interaction). Body weight (BW) and heart girth (HG) in group B was higher than in C during the experimental period (p = 0.07 and 0.01 for the treatment × week interaction, respectively). The duration of the weaning period in group B was shorter than in group C (p = 0.02). β‐hydroxybutyrate (BHBA) was higher in group B than in C (p = 0.04). Insulin like growth factor‐1 (IGF‐1) concentrations tended to be higher in group B than in C (p = 0.07 for treatment × week interaction). Our results show that butyrate supplementation in antibiotic‐free milk replacer and starter exerted positive effects on growth performance in suckling calves.     

Differences in flowering phenology and seed production of four morning glory (Ipomoea) species in Japan

The spread of morning glory (Ipomoea spp.) in soybean fields in Japan has severely decreased soybean yield. Yet, current control measures do not control the proliferation of Ipomoea spp. As little is known about the flowering period and seed production among the different invading Ipomoea spp., it is challenging to create targeted control measures based on ecological characteristics. This study aimed to reveal the characteristics of the flowering phenology and seed production of four morning glory species, namely, Ipomoea coccinea L. (red morning glory), Ipomoea lacunosa L. (pitted morning glory), Ipomoea hederacea L. Jacq. var. integriuscula A. Gray (entireleaf morning glory), and Ipomoea triloba L. (three-lobe morning glory). Between 2017 and 2019, the four selected study species were grown under similar conditions of soil quality, irrigation, and environmental influences and their flowering phenology and seed data were recorded. The flowering period ranged from 36 to 40 days, and the initial flowering of I. triloba was approximately 2 weeks later than the others. I. coccinea had the highest flowering number and seed production, followed by I. lacunosa, I. triloba, and I. hederacea var. integriuscula. The fruit setting rate of I. triloba decreased later in the reproductive stage but tended to increase as the daily mean temperature increased on each flowering day. Thus, we report that the flowering phenology and seed production differed greatly among the Ipomoea spp. These findings can provide crucial insights into designing targeted species-specific control measures against the spread of Ipomoea spp. in Japan.     

Estrous cycle based on blood progesterone profiles and changes in vulvar appearance of Malayan tapirs (Tapirus indicus)

The progesterone (P(4)) profiles and macroscopic vulvar changes of female Malayan tapirs were investigated in order to understand their fundamental reproductive physiology and to search for visual indicators of estrus. Blood was collected once or twice a week from seven female Malayan tapirs kept at four zoos. Serum or plasma P(4) concentrations were determined by radioimmunoassay. The P(4) concentrations changed cyclically throughout the years, and a total of 56 cycles was confirmed in the seven females. The length of the estrous cycle based on the P(4) profiles was 43.6+/-2.0 days; however, this mean includes great variation in length, from 21 to 84 days. Mucous discharge from the vulva and vulvar swelling were seen when the P(4) concentrations were low before the beginning of a rise in most cases. In conclusion, captive female Malayan tapirs have variations of approximately 1 to 3 months in estrous cycle length, and visual changes in the vulva are helpful in estimating estrus in female Malayan tapirs.     

Factor analysis of risk-taking behavior in forest work

In this study, the data obtained by a questionnaire survey were analyzed to examine the difference with the type of occupation, consistency in each situation, the inductive and restraint factors of the risk-taking behavior in the forest work to elucidate the risk-taking behavior in forest work. The results showed that (1) the Kyushu Forest Cooperative Association’s staff members underestimate the risk, but have a strong recognition of “law observance,” (2) the supervisor or instructor has low consciousness of risk, (3) as the inductive factor of behavior, one’s own benefit in the daily situation is given the highest priority, but risk is the most important factor in situations concerning forest work and forest machine operation and handling, irrespective of the accuracy of the risk evaluation, (4) as the restraint factor of behavior, although there is a difference in the risk evaluation whether large or small, risk is given priority by all workers, and (5) the dare probability of each risk-taking behavior is highly correlated with the risk evaluation of the behavior. Among others, low consciousness of the supervisor or instructor concerning safety management is pointed out as a main factor in worker’s accidents, and reconsideration of the relationship between the supervisor or instructor and workers is necessary.     

Low nitrogen availability and shallow plow layer decrease sugarcane (Saccharum officinarum L.) productivity in Kitadaito Island,Japan

Sugarcane is the main crop in Kitadaito Island, Okinawa, Japan. The average yield in Kitadaito Island for the past 30 years (1985–2015) was 44 Mg ha^?1, 31% lower than that (64 Mg ha^?1) in Okinawa Prefecture. Among the reasons for the low sugarcane productivity in Kitadaito Island, the effects of soil and cultivation factors have not been sufficiently studied. The objectives of this study were i) to evaluate the effects of soil physical and chemical properties and cultivation factors on sugarcane yield and sugar content in 50 fields of Kitadaito Island, and ii) to clarify factors affecting the sugarcane yield and sugar content. Statistical analysis of classification and regression trees (CART) showed that the sugarcane yield was low in fields with low available nitrogen (less than 16.5 mg kg^?1) and with shallow plow layer depth (less than 29 cm). The soil available nitrogen had a significant positive correlation with soil humus (r = 0.39, P < 0.05). Two-times of subsoiling in the field before planting and during growing periods resulted in the significant increase of the depth of plow layer, compared to the fields with single and no subsoiling. A high sugar content was obtained in fields with exchangeable Mg more than 3.8 cmol_c kg^?1 and pH(H₂O) less than 7.2. Our study therefore concluded that soil management practices for sugarcane production in Kitadaito Island should primarily be focused on available nitrogen, plow layer depth, and soil exchangeable Mg and pH.     

Ruminal epithelial insulin‐like growth factor‐binding proteins 2, 3, and 6 are associated with epithelial cell proliferation

The aim of this study was to identify factors that regulate ruminal epithelial insulin‐like growth factor‐binding protein (IGFBP) expression and determine its role in rumen epithelial cell proliferation. Primary bovine rumen epithelial cells (BREC) were incubated with short‐chain fatty acids (SCFAs) at pH 7.4 or 5.6, lactate, lipopolysaccharide (LPS), insulin‐like growth factor‐I (IGF‐I), ‐II (IGF‐II), or recombinant bovine IGFBP2 (rbIGFBP2). The mRNA expression levels of IGFBP in BREC were analyzed using quantitative real‐time polymerase chain reaction (qRT‐PCR). The proliferation rate of BREC was analyzed using a WST‐1 assay. IGFBP2 gene expression tended to be lower with SCFA treatment (p < .1), and IGFBP6 gene expression was significantly lower with SCFA treatment (p < .05). IGFBP3 and IGFBP6 gene expression tended to be higher with d ‐Lactate treatment (p < .1). IGFBP3 gene expression was significantly higher (p < .05) with LPS treatment. BREC treated with IGF‐I grew more rapidly than vehicle control‐treated cells (p < .01); however, recombinant bovine rbIGFBP2 inhibited IGF‐I‐induced proliferation. IGF‐II and/or rbIGFBP2 did not affect BREC proliferation. Taken together, SCFA treatment decreased IGFBP2 and IGFBP6 expression in rumen epithelial cells, and lower expression of these IGFBP might promote rumen epithelial cell proliferation by facilitating IGF‐I.     

Phyto-purification of livestock-derived organic waste by forage rice under subtropical climate

Soil and water pollution caused by organic waste is a concern for livestock-breeding areas. Nitrogen balance in a paddy-field water-purifying system in which cattle feces were applied was studied for 4 years to assess the suitability of the system for a subtropical area, Japan. Three successive harvestings using ratoon of forage rice following one rice transplanting were conducted with chemical fertilizer and high and low rates of cattle-feces application. Nitrogen load was 81.3–495.0 kg N ha^?1 year^?1, while nitrogen uptake was highly dependent on the yield of the first harvesting. Annual variation of forage rice yields was large, ranging from 15.5 to 26.8 Mg ha^?1 owing to fluctuation in the yield at second and later harvestings. On average, nitrogen was lost by leaching at a rate of 2.3–3.4 kg N ha^?1 year^?1. The nitrogen content in soil at a depth of 0–5 cm increased up to 12.2 kg N ha^?1 over the 4-year period compared with that before the field experiment. However, continuous application of cattle feces could slightly increase the nitrogen content in soil at a depth greater than 35 cm. Our results showed the ability of flooded forage rice to remove nitrogen at up to 320.1 kg ha^?1 year^?1 for a field to which cattle feces were applied. Further investigation is needed to produce a high and stable yield at second harvesting each year, to prevent the accumulation of soil nitrogen, and to assess gaseous nitrogen loss.     

Nitrogen dynamics of anaerobically digested slurry used to fertilize paddy fields

To determine nitrogen (N) fate and environmental impact of applying anaerobic digestion slurry (ADS) to rice paddy (Oryza sativa L.), a field experiment was established using three treatments based on contrasting N application rate. The ADS (with ammonium-N accounting for >80 % of total N) treatment at a conventional application rate of 270 kg N?ha^?1 was compared to a negative control (no N fertilizer) and a positive control of urea applied at 270 kg N?ha^?1. The N budget showed the following distribution of applied N from ADS and urea: 41.3?±?5.1 % for ADS and 36.6?±?4.4 % for urea recovered by the rice plant (including straw, grain, and root), 16.4?±?3.7 % for ADS and 7.4?±?1.8 % for urea lost via ammonia volatilization, 0.26?±?0.15 % for ADS and 0.15?±?0.12 % for urea lost by direct N₂O emission, 1.9?±?0.5 % for ADS and 2.3?±?0.8 % for urea leached downward, 0.70?±?0.15 % for ADS and 0.67?±?0.12 % for urea discharged with floodwater drainage, and 39.4?±?8.4 % for ADS and 53.0?±?9.1 % for urea retained by soil or lost by N₂ emission. Compared to urea application, ADS application impacts the environment mainly through gaseous N losses rather than water N losses. ADS application had a positive impact on rice grain yield and reduced chemical fertilizer use. Considering the wide distribution of paddy fields and the ever-increasing quantities of ADS, ADS may serve as a valuable N source for rice cultivation, although mitigating ammonia and N₂O losses should be further investigated.