全文获取类型
收费全文 | 452篇 |
免费 | 13篇 |
专业分类
林业 | 29篇 |
农学 | 11篇 |
基础科学 | 1篇 |
60篇 | |
综合类 | 10篇 |
农作物 | 19篇 |
水产渔业 | 42篇 |
畜牧兽医 | 264篇 |
园艺 | 2篇 |
植物保护 | 27篇 |
出版年
2023年 | 1篇 |
2022年 | 9篇 |
2021年 | 15篇 |
2020年 | 5篇 |
2019年 | 8篇 |
2018年 | 4篇 |
2017年 | 8篇 |
2016年 | 13篇 |
2015年 | 18篇 |
2014年 | 28篇 |
2013年 | 38篇 |
2012年 | 37篇 |
2011年 | 43篇 |
2010年 | 12篇 |
2009年 | 15篇 |
2008年 | 24篇 |
2007年 | 34篇 |
2006年 | 19篇 |
2005年 | 27篇 |
2004年 | 26篇 |
2003年 | 29篇 |
2002年 | 30篇 |
2001年 | 8篇 |
2000年 | 3篇 |
1999年 | 4篇 |
1998年 | 2篇 |
1997年 | 1篇 |
1993年 | 2篇 |
1992年 | 1篇 |
1979年 | 1篇 |
排序方式: 共有465条查询结果,搜索用时 0 毫秒
51.
Detection of the esp gene in high-level gentamicin resistant Enterococcus faecalis strains from pet animals in Japan 总被引:1,自引:0,他引:1
We investigated the prevalence of the esp gene and the susceptibility to gentamicin in Enterococcus faecalis and E. faecium strains obtained from pet animals. Nine of 30 E. faecalis and 2 of 38 E. faecium strains from the pet animals had the esp gene. Three esp-positive E. faecalis strains, which were isolated from two dogs and a cat, showed gentamicin MICs of > or =256 microg/ml and harbored the high-level gentamicin resistance (HLGR) gene, aac(6')-Ie-aph(2')-Ia. Of the nine esp-positive E. faecalis strains, five, including the three strains with the HLGR gene, were closely related by numerical analysis of PFGE patterns. Longitudinal investigation needs to elucidate whether the HLGR gene was incorporated into a subpopulation of the esp-positive E. faecalis. 相似文献
52.
Ishida R Masuda K Kurata K Ohno K Tsujimoto H 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2004,18(1):25-30
Lymphocyte blastogenic responses against food allergens in dogs with food hypersensitivity were evaluated in this study. Eleven dogs with food hypersensitivity, based on food elimination and oral food provocation tests and allergic responses to food allergens, were examined by various tests such as intradermal testing, antigen-specific IgE testing, and lymphocyte blastogenic responses. The number and kinds of food allergens identified as positive by these tests were compared with the offending food allergens that were found in an oral food provocation test. In 9 (82%) of the 11 dogs with food hypersensitivity, there was close agreement for positive allergens between the results of lymphocyte blastogenic responses and oral food provocation test; however, there was little agreement for intradermal and IgE testing of the positive allergens with those of the oral food provocation test (11% and 31%, respectively). In the 9 dogs, the stimulation indices of lymphocyte blastogenic responses increased to 2.0-10.1 upon food provocation but decreased significantly to 0.7-1.4 upon feeding the elimination diet until clinical signs disappeared. These results indicate that lymphocyte blastogenic responses may fluctuate because of exposure to offending food allergens in dogs with food hypersensitivity. Lymphocytes reactive to food allergens may play an important role in the pathogenesis of food hypersensitivity in dogs. 相似文献
53.
Comparison of virulence of various hantaviruses related to hemorrhagic fever with renal syndrome in newborn mouse model 总被引:1,自引:0,他引:1
Lokugamage K Kariwa H Lokugamage N Iwasa M Hagiya T Araki K Tachi A Mizutani T Yoshimatsu K Arikawa J Iwasaki T Takashima I 《The Japanese journal of veterinary research》2004,51(3-4):143-149
The virulence of hantaviruses that are antigenically related but have different genetic characteristics from the prototype of hantavirus, Hantaan (HTN) virus, was examined in newborn mice. The H5 and B78 strains of the Amur (AMR) genotype, the Bao14 strain of the Far East (FE) genotype, and the 76-118 strain of HTN virus were inoculated subcutaneously (1focus-forming unit; FFU) into newborn mice. All of the AMR and FE genotype viruses inoculated mice were died by 16 days post-infection (dpi) and 21 dpi, respectively, while 50% of the HTN virus inoculated mice survived until 30 dpi. The AMR and FE genotype viruses inoculated mice had high viral titers in the lung (1.3x10(6) to 1.3x10(8) FFU/gram [g] tissue) , brain (2.1x10(7) to 1.2x10(9) FFU/g tissue), and kidney(2.5x10(5) to 1.6x10(7) FFU/g tissue), and showed a detectable level of antibodies (titers 1:16-1:32) at 14 dpi. In contrast, the HTN virus infected mice had viruses only in the lungs at low titers (1.1-5.3x10(5) FFU/g tissue). Observations of body-weight changes revealed that the AMR and FE genotype viruses inoculated mice had lower growth rates than the HTN virus inoculated mice. These data suggest that the AMR and FE genotype viruses are more virulent than the HTN virus in newborn mice. 相似文献
54.
Kageyama S Yoshida I Kawakura K Chikuni K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(5):509-514
A novel repeated sequence specific to male cattle was identified and named S4. S4 is a highly repetitive sequence and is a 1.5 kb repeating unit that contains various internal repeated sequences. FISH analysis showed that S4 is localized on the whole long arm and the proximal region of the short arm of the Y chromosome. We found that a PCR primer set for S4 amplified a male-specific 178 bp product in addition to a 145 bp product common to both male and female cells. Although the origin of the 145 bp product is unknown, it acts as a positive internal control in practical embryo sexing. Due to the high copy number of S4, PCR required only 0.5 pg purified DNA for accurate amplification. This made it possible to reduce the amount of biopsy sample required for embryo sexing and thus result in less damage to embryos manipulated. These studies indicate that embryo sexing based on the S4 sequence is accurate and sensitive. 相似文献
55.
Katayama H Hori M Sato K Kajita M Ozaki H Karaki H Ohashi K Kai C 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(4):409-415
Several studies have indicated that viruses require a specific cytoskeletal structure for replication in host cells. In this study, we examined the role of actin fiber in the replication of canine distemper virus (CDV), belonging to the Morbillivirus genus of the family Paramyxoviridae. For this purpose, we used two actin depolymerizing agents, cytochalasin-D (C-D) and mycalolide-B (ML-B). In Vero cells, C-D disrupted actin fibers distributed in the cytosol, but peripheral actin fibers remained intact. On the other hand, ML-B completely disrupted the actin fibers distributed in both areas. Treatment of Vero cells with C-D or ML-B inhibited the replication of CDV. Double staining of CDV-infected Vero cells with antibody to N-protein and rhodamine-phalloidin revealed the presence of N-protein in mid-cytoplasm. However, the N-protein was specifically localized at the submembrane region in the presence of C-D, whereas it was clustered in the presence of ML-B. Viral mRNA levels of N- and H-proteins were rather increased by treatment with C-D or ML-B. The treatment with ML-B strongly inhibited N-protein expression, whereas C-D only slightly inhibited N-protein expression. These results suggest that actin microfilaments distributed in the cytoplasm and on the membrane region in host cells may have a different role in the process of CDV replication. 相似文献
56.
Miwako Ito Wakako Maruyama-Funatsuki Tatsuya M. Ikeda Zenta Nishio Koichi Nagasawa Tadashi Tabiki Hiroaki Yamauchi 《Breeding Science》2012,62(4):340-347
The relationship between characterictics of flour of common wheat varieties and fresh pasta-making qualitites was examined, and the fresh pasta-making properties of extra-strong varieties that have extra-strong dough were evaluated. There was a positive correlation between mixing time (PT) and hardness of boiled pasta, indicating that the hardness of boiled pasta was affected by dough properties. Boiled pasta made from extra-strong varieties, Yumechikara, Hokkai 262 and Hokkai 259, was harder than that from other varieties and commercial flour. There was a negative correlation between flour protein content and brightness of boiled pasta. The colors of boiled pasta made from Yumechikara and Hokkai 262 grown under the condition of standard manuring culture were superior to those of boiled pasta made from other varieties. Discoloration of boiled pasta made from Yumechikara grown under the condition of heavy manuring culture was caused by increase of flour protein content. On the other hand, discoloration of boiled pasta made from Hokkai 262 grown under the condition of heavy manuring culture was less than that of boiled pasta made from Yumechikara. These results indicate that pasta made from extra-strong wheat varieties has good hardness and that Hokkai 262 has extraordinary fresh pasta-making properties. 相似文献
57.
Five oleanolic acid triterpenoid saponins (OTS-1, 2, 3, 4 and 5) were isolated from the rhizome of Anemone raddeana. The effect of these triterpenoid saponins on stimulus-induced superoxide generation in human neutrophils was assayed by measuring the reduction of ferricytochrome c using a dual-beam spectrophotometer. The phosphorylation of neutrophil proteins, and translocation of p67(phox), p47(phox) and Rac to plasma membrane were investigated using specific monoclonal antibodies. The five oleanolic acid triterpenoid saponins used in this experiment suppressed N-formyl-methionyl-leucyl-phenylalanine (fMLP)-induced superoxide generation in a concentration-dependent manner. OTS-1, 2 and 4 suppressed phorbol 12-myristate 13-acetate (PMA)- and arachidonic acid (AA)-induced superoxide generation in a concentration-dependent manner, but OTS-3 and 5 showed no effect. fMLP- and PMA-induced tyrosyl or serine/threonine phosphorylation, and fMLP-, PMA- and AA-induced translocation of p67(phox), p47(phox) and Rac to plasma membrane were in parallel with the suppression of the stimulus-induced superoxide generation. 相似文献
58.
Shiaki TAKAGI Shunji HAYASHI Koichi TAKAHASHI Hiroshi ISOGAI Lanlan BAI Hiroshi YONEYAMA Tasuke ANDO Kumiko ITO Emiko ISOGAI 《Animal Science Journal》2012,83(6):482-486
A bovine myeloid antimicrobial peptide (BMAP‐28) is a member of the cathelicidin family which is included in the innate immune system of mammals. Recently, there have been many studies about antimicrobial peptides. This study aims to clarify whether BMAP‐28 has bactericidal activity against methicillin‐resistant Staphylococcus aureus (MRSA) and compares its activity against methicillin‐susceptible S. aureus (MSSA) and MRSA. We found that the peptide was effective in killing MRSA (minimal inhibitory concentration (MIC) range; 5–20 µg/mL). It was also revealed that MSSA (MIC range; 1.25–20 µg/mL) had two levels of susceptibility to BMAP‐28. We also examined the effect of BMAP‐28 on bacterial shape to visually show its activity. After exposure to the peptide, both MSSA and MRSA cells showed the morphological changes on their surfaces. Our results indicate that BMAP‐28 is a promising candidate for medicine against drug‐resistant bacteria. 相似文献
59.
Naoko Emura Kazuto Kawakami Tomohiro Deguchi Koichi Sone 《Journal of Forest Research》2012,17(4):352-359
Some introduced frugivorous birds disperse introduced plants and are thus a substitute for extinct native birds. Introduced birds have negative and/or infrequently positive effects on local ecosystems. It is important for management of the ecosystems to understand the relationships between native and introduced frugivorous and plant species. In this study, we elucidated these relationships in Mukojima Island, the Bonin Islands, where was anthropologically deforested and Japanese White-eye Zosterops japonicus and some plants were already introduced. We examined the habitat selection of frugivorous birds, actual dispersed seeds in bird feces, and the distribution of the potentially dispersed plant species. The Japanese White-eye and the native, Blue Rockthrush Monticola solitarius, were dominant on this island. The former mainly used the forest area and dispersed only small seeds and frequently introduced plant seeds. The latter mainly used the open area and dispersed both small and large seeds. Some small-seed plants occurred not only in the forest but also in the open area. Their seedlings were distributed farther from their adult trees than the large-seed species. These indicate that small-seed plants would be more spread than the large-seed plants because the two bird species disperse their seeds in different environments. This introduced bird species may be important in vegetation recovery, although it may contribute to the distribution of introduced plants on this island. 相似文献