全文获取类型
收费全文 | 519篇 |
免费 | 13篇 |
专业分类
林业 | 29篇 |
农学 | 11篇 |
基础科学 | 1篇 |
67篇 | |
综合类 | 11篇 |
农作物 | 19篇 |
水产渔业 | 46篇 |
畜牧兽医 | 313篇 |
园艺 | 3篇 |
植物保护 | 32篇 |
出版年
2023年 | 1篇 |
2022年 | 18篇 |
2021年 | 22篇 |
2020年 | 7篇 |
2019年 | 8篇 |
2018年 | 4篇 |
2017年 | 8篇 |
2016年 | 14篇 |
2015年 | 24篇 |
2014年 | 33篇 |
2013年 | 41篇 |
2012年 | 39篇 |
2011年 | 45篇 |
2010年 | 14篇 |
2009年 | 22篇 |
2008年 | 25篇 |
2007年 | 38篇 |
2006年 | 22篇 |
2005年 | 27篇 |
2004年 | 29篇 |
2003年 | 31篇 |
2002年 | 31篇 |
2001年 | 10篇 |
2000年 | 5篇 |
1999年 | 4篇 |
1998年 | 2篇 |
1997年 | 1篇 |
1994年 | 1篇 |
1993年 | 2篇 |
1992年 | 1篇 |
1979年 | 1篇 |
1963年 | 1篇 |
1957年 | 1篇 |
排序方式: 共有532条查询结果,搜索用时 0 毫秒
91.
Glomerular lipidosis is a disease characterized by lipid accumulation in mesangial cells but that has not been fully investigated in avian species. We examined four wild and two laboratory-reared Japanese rock ptarmigans (Lagopus mutus japonicus)--an endangered avian species--presenting vacuolar deposits in the glomeruli. All cases had vacuolar deposits in the glomeruli. In the wild cases, fewer than 30% of all glomeruli were affected, compared with more than 90% in the laboratory-reared cases. In the wild cases, most deposits were mild and restricted to the mesangial areas of glomeruli. In the laboratory-reared cases, nearly all of the deposits covered entire glomeruli. Electron microscopy of mild deposits revealed vacuoles in the cytoplasm of mesangial cells. These vacuoles were positive for Sudan III, Sudan black B, oil red O, Nile blue, periodic acid-Schiff, Schultz test, and digitonin stain and were negative for performaric acid-Schiff stains. Based on these results, we diagnosed the glomerular lesion as glomerular lipidosis caused by uptake of low-density lipoprotein in mesangial cells. Except for one wild case, all cases exhibited renal tubular oxalosis. The severity of tubular oxalosis tended to be related to the severity of glomerular lipidosis: In cases of mild glomerular lipidosis, tubular oxalosis was also mild or absent. We therefore diagnosed the primary lesion as glomerular lipidosis accompanied by tubular oxalosis. The four wild cases came from different zones and therefore had no opportunities to interbreed and no common relatives. We believe these data support the hypothesis that glomerular lipidosis is a disease of the general population ofJapanese rock ptarmigans. This is the first report of glomerular lipidosis accompanied by renal tubular oxalosis in an avian species. 相似文献
92.
93.
The beneficial effects of horse trekking on autonomic nervous activity in experienced rider with no disability 下载免费PDF全文
Akihiro Matsuura Haruhiro Maruta Tomohiko Iwatake Takashi Kumagai Toshihiko Nakanowatari Koichi Hodate 《Animal Science Journal》2017,88(1):173-179
The aim of this study was to determine whether autonomic nervous activity of a rider with no disability was altered by one practical and applicable horse trekking (HT) exercise. Changes in autonomic nervous activity were analyzed by heart rate variability (HRV). Twenty‐three participants with no disability rode horses along a predetermined HT course at trotting and walking for 60 min. HRV was sampled at 60 min before and immediately, following 60 min, and 120 min after HT. As a control, the same measurements were performed for 22 age‐matched participants during their rest. Only in the HT group, the value of normalized unit in high frequency component (HF nu), an index of parasympathetic nervous activity, was higher at 120 min after treatment than before HT (P < 0.05). The low / high frequency ratio (LF / HF), believed to reflect sympathetic nervous activity, was lower in the HT group than those in the control group at 60 min (P < 0.05) and 120 min after treatment (P < 0.01). These findings suggest that a single HT shifted the autonomic nervous balance of a rider toward parasympathetic dominance. The results obtained by the present study could accelerate the use of horses for human health. 相似文献
94.
Shuhei ENJOJI Ryotaro YABE Nobuyuki FUJIWARA Shunya TSUJI Michael P. VITEK Takuya MIZUNO Takayuki NAKAGAWA Tatsuya USUI Takashi OHAMA Koichi SATO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1451-1456
Canine melanoma is one of the most important diseases in small animal medicine.
Protein phosphatase 2A (PP2A), a well conserved serine/threonine phosphatase, plays a
critical role as a tumor suppressor. SET/I2PP2A is an endogenous inhibitor for PP2A, which
directly binds to PP2A and suppresses its phosphatase activity. Elevated SET protein
levels have been reported to exacerbate human tumor progression. The role of SET in canine
melanoma, however, has not been understood. Here, we investigated the potential
therapeutic role for SET inhibitors in canine melanoma. The expression of SET protein was
observed in 6 canine melanoma cell lines. We used CMeC-1 cells (primary origin) and CMeC-2
cells (metastatic origin) to generate cell lines stably expressing SET-targeting shRNAs.
Knockdown of SET expression in CMeC-2, but not in CMeC-1, leads to decreased cell
proliferation, invasion and colony formation. Phosphorylation level of p70 S6 kinase was
decreased by SET knockdown in CMeC-2, suggesting the involvement of mTOR (mammalian target
of rapamycin)/p70 S6 kinase signaling. The SET inhibitors, OP449 and FTY720, more
effectively killed CMeC-2 than CMeC-1. We observed PP2A activation in CMeC-2 treated with
OP449 and FTY720. These results demonstrated the potential therapeutic application of SET
inhibitors for canine melanoma. 相似文献
95.
Koichi Ojima 《Animal Science Journal》2019,90(7):801-807
Skeletal muscle consists of bundles of myofibers containing millions of myofibrils, each of which is formed of longitudinally aligned sarcomere structures. Sarcomeres are the minimum contractile unit, which mainly consists of four components: Z‐bands, thin filaments, thick filaments, and connectin/titin. The size and shape of the sarcomere component is strictly controlled. Surprisingly, skeletal muscle cells not only synthesize a series of myofibrillar proteins but also regulate the assembly of those proteins into the sarcomere structures. However, authentic sarcomere structures cannot be reconstituted by combining purified myofibrillar proteins in vitro, therefore there must be an elaborate mechanism ensuring the correct formation of myofibril structure in skeletal muscle cells. This review discusses the role of myosin, a main component of the thick filament, in thick filament formation and the dynamics of myosin in skeletal muscle cells. Changes in the number of myofibrils in myofibers can cause muscle hypertrophy or atrophy. Therefore, it is important to understand the fundamental mechanisms by which myofibers control myofibril formation at the molecular level to develop approaches that effectively enhance muscle growth in animals. 相似文献
96.
Yasuyuki TANAHASHI Ban WANG Yuri MURAKAMI Toshihiro UNNO Hayato MATSUYAMA Hiroshi NAGANO Seiichi KOMORI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(2):203-211
In order to investigate the effects of (SKF), which is a non-selective cationic channel blocker, on
K+ channel currents, we recorded currents through ATP sensitive K+ (IKATP),
voltage-gated K+ (IKv) and Ca2+ activated K+ channels
(IBK) in the absence and presence of SKF in single small intestinal myocytes of mice with
patch-clamp techniques. SKF (10 µM) reversibly abolished IKATP that was induced by
cromakalim (10 µM), which is a selective ATP sensitive K+ channel opener. These
inhibitory effects were induced in a concentration-dependent and voltage-independent manner. The 50%
inhibitory concentration (IC50) was 0.85 µM, which was obviously lower than that
reported for the muscarinic cationic current. In addition, SKF (1 µM ≈ the IC50
value in IKATP suppression) reversibly inhibited the IKv that was induced by repetitive
depolarizing pulses from −80 to 20 mV. However, the extent of the inhibitory effects was only ~30%. In
contrast, SKF (1 µM) had no significant effects on spontaneous transient IBK and
caffeine-induced IBK. These results indicated that SKF inhibited ATP sensitive K+
channels and voltage-gated K+ channels, with the ATP sensitive K+ channels being more
sensitive than the voltage-gated K+ channels. These inhibitory effects on K+ channels
should be considered when SKF is used as a cationic channel blocker. SKF96365相似文献
97.
Maho NAKAZAWA Hirotaka TOMIYASU Kanako SUZUKI Hajime ASADA Aki FUJIWARA-IGARASHI Yuko GOTO-KOSHINO Aki OHMI Koichi OHNO Michio FUJITA Hajime TSUJIMOTO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(3):456
Nasal lymphoma (NL) is the most common nasal tumor in cats, and radiotherapy, chemotherapy, or a combination of these treatments have been described as the treatment for this disease. However, the previous studies included various machines and protocols of radiotherapy. Therefore, we aimed to retrospectively compare the prognosis among cases treated with palliative hypofractionated radiotherapy, chemotherapy, and a combination of them with united machine and protocol of radiotherapy. When compared overall survival and progression free survival, there was no significant difference among these three groups. The data of this study suggested that similar efficacy could be achieved by palliative hypofractionated radiotherapy, chemotherapy, or a combination of them. 相似文献
98.
Toki Nishiyama Nobuhisa Ashida Koichi Nakagawa Shun Iwatani Naoyuki Yamamoto 《The Journal of Poultry Science》2021,58(2):138
Among the reported probiotic Bacillus strains, B. subtilis C-3102 has the unique potential to improve feed uptake under stress conditions in the broilers, piglets, and cows. In this study, we sought to evaluate the protective effect of feed additive probiotic Bacillus subtilis C-3102 against Salmonella enterica infection of specific pathogen-free (SPF) chicks in floor pens in two experiments. In the experiment-1, the chicks in the control group (n=32) were fed a basal diet and those in the C-3102 group (n=32) were fed a basal diet supplemented with 1×106 CFU/g of feed for 28 days. On day 7 post-challenge with S. enterica, there was no significant change in the body weight between both the groups throughout the test period, whereas detection rates of S. enterica in the C-3102 group were significantly lower in the cecum and liver on days 21 and 14 post-challenge, respectively. In the experiment-2, minimum dosage of C-3102 cells required to protect Salmonella infection was evaluated using 3 dosages. Chicks were divided into four groups, fed with different dosages of C-3102 (1×106, 5×105, 3×105, and 0 CFU/g of feed), and challenged with S. enterica (2.8×108 CFU/chicken). S. enterica infection was completed within 7 days post- challenge and was almost excluded from the liver and spleen on day 21 post- challenge in the control group. Average values showed a trend for higher infection rates in the control group >3×105>5×105>1×106 CFU/g on days 14 and 21 post-challenge. These results suggest that B. subtilis C-3102 supplementation has the potential to reduce S. enterica infection rates and/or to accelerate the exclusion of S. enterica from the chicks. 相似文献
99.
Koichi Watanabe Yutaka Ishizaka Yukiya Minami Koji Yoshida 《Water, air, and soil pollution》2001,130(1-4):1559-1564
Measurements of peroxide concentrations in fog water were conducted near the summit of Mt. Norikura (altitude, 2770m) in central Japan, and at the midslope of Mt. Oyama (altitude, 680m), southwest of the Kanto Plain. The concentrations of peroxide at Mt. Norikura, far from industrial regions, ranged from 3 to 120 µ M during the summer and early autumn in 1993. The potential capacity for SO2 oxidation appears to be very high near the summit of Mt. Norikura. Analysis of the chemical composition of three-stage size-fractionated fog water samples collected at Mt. Norikura showed that the concentrations of peroxide were apparently independent of droplet size, whereas the concentrations of chemical constituents mainly derived from secondary aerosols and the acidity were higher in smaller droplets. Peroxide concentrations in fog water were low (< 5 µ M) at Mt. Oyama, located near heavy industrial areas, and lower than those in rain water sampled simultaneously (0.2–33 µ M). Especially, peroxide was scarcely detected in strongly acidic fogs (< 0.2 µ M). Peroxide might have been decomposed by SO2 (S(IV)) oxidation in the aqueous-phase. 相似文献
100.
Occurrence of New Powdery Mildew on Greenhouse Tomato Cultivars 总被引:2,自引:0,他引:2
Yoshinori MATSUDA Koichi KASHIMOTO Yoshihiro TAKIKAWA Rika AIKAMI Teruo NONOMURA Hideyoshi TOYODA 《Journal of General Plant Pathology》2001,67(4):294-298
During a year-round survey on the occurrence of powdery mildew on greenhouse-cultivated tomato plants, the disease was most
severe in June and July. All tomato plants (45 commercial cultivars and 11 breeding lines) tested were infected with the pathogen
but had different degrees of susceptibility. The pathogen was epiphytic and produced white, round pustules mainly on leaves
of tomato plants. The pathogen produced conidia singly on conidiophores and forked appressoria on inoculated tomato leaves
and seemed to be an Oidium sp. of Erysiphe polygoni type.
Received 18 December 2000/ Accepted in revised form 22 July 2001 相似文献