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71.
David Gamarra Noelia Aldai Aisaku Arakawa Marian M. de Pancorbo Masaaki Taniguchi 《Animal Science Journal》2021,92(1):e13521
Sterol regulatory element-binding factor 1 (SREBP1) plays an important role in the lipogenesis which affects fatty acid (FA) composition in backfat and consequently influences beef nutritional quality. This study analyzed the association of 84 bp-indel, both short (S) and long (L) alleles in intron 5 of SREBP1, with FA composition and gene expression of SREBP1 in backfat of northern Spanish beef breeds (Pirenaica, Salers and Holstein-Friesian). Phylogenetic analysis suggests that 84 bp-indel of ruminants is a highly conserved region compared with those in the full-length sequence of intron 5 or mRNA of SREBP1 among species. Overall, higher content of polyunsaturated FAs was observed in SL genotype compared to LL genotype of 84 bp-Indel (p < .05). In particular, in Pirenaica, SL genotype was associated with a higher content of stearic (18:0), α-linolenic (18:3n-3) acid, and total n-3 content (p < .05). However, the gene expression of SREBP1 did not differ among genotypes of 84 bp-Indel (p > .05). 相似文献
72.
Yoko Sato Ryota Kuriwaki Shiki Hagino Megumi Shimazaki Rentsenkhand Sambuu Maki Hirata Fuminori Tanihara Mitsuhiro Takagi Masayasu Taniguchi Takeshige Otoi 《Reproduction in domestic animals》2020,55(2):209-216
In Mongolia, yak (Bos grunniens) are able to live in alpine areas and their products greatly influence the lives of the local people. Increased vigour in hybridized yak and cattle can offer benefits for livestock farmers. However, male hybrids show reproductive defects resulting from spermatogenesis arrest, affecting the conservation and maintenance of dominant traits in the next generation. The underlying mechanisms involved in hybrid cattle–yak infertility have recently been investigated; however, the genetic cause is still unclear. Androgens and androgen receptor (AR) signalling are required for spermatogenesis. We, therefore, evaluated the expression of AR, 3β-hydroxysteroid dehydrogenase (3βHSD) and 5α-reductase 2 (SRD5A2) in Leydig cells to investigate their function in cattle–yak spermatogenesis. Testicular tissues from yaks (1–3 years old) and hybrids (F1–F3, 2 years old) were collected and subjected to immunohistochemistry and image analyses to investigate the expression of each parameter in the Leydig cells. After maturation at 2 years, the expression levels of AR increased and the levels of 3βHSD decreased, but the SRD5A2 levels remained constant in yak. However, the cattle–yak hybrid F2 showed immature testicular development and significantly different expression levels of AR and 3βHSD compared with mature yak. These results suggest that the decreased expression of AR and increased expression of 3βHSD in the Leydig cells of cattle–yak hybrid testes may represent one of the causes of infertility. Our study might help in solving the problem of infertility in crossbreeding. 相似文献
73.
Shinya Ishihara Fukuhiro Yamasaki Pham Hai Ninh Nguyen Cong Dinh Aisaku Arakawa Masaaki Taniguchi Ngo Thi Kim Cuc Satoshi Mikawa Masaru Takeya Kazuhiro Kikuchi 《Animal Science Journal》2020,91(1)
Although there are a number of Vietnamese native pig (VnP) populations, some are on the verge of extinction, and therefore adequate management and conservation are necessary. In this study, we conducted a field survey of VnP populations and analyzed interrelationships among their characteristics. We also established a relational database for management of field data on these populations. For data collection, we conducted interviews with farmers and visual inspection of 32 VnP populations in 22 provinces of Vietnam, as well as taking photographs of individual animals. Data on the characteristics of VnP populations were subjected to multiple correspondence analysis (MCA). For establishment of the database, normalization and table partitioning were performed to eliminate redundancy and ensure consistency of the collected data items. Passport data, characteristics data, and image data were collected from a total of 1,918 VnPs and entered as a normalized table. Upon MCA, most of the populations were not separated from each other, but the Mong Cai, O Lam, and Chu Prong populations were separated from the other populations. Thus, we have constructed a relational database from comprehensive information on the characteristics of VnP populations. 相似文献
74.
Yuichiro Hiraoka Noritsugu Kuramoto Mineko Ohira Masanori Okamura Toru Taniguchi Yoshitake Fujisawa 《Journal of Forest Research》2011,16(6):509-517
Rhus succedanea L. is cultivated in Japan for the wax that can be extracted from its fruits. We determined genetic data for specific traits
associated with wax production (fruit number per cluster, FN, fruit weight, FW, number of clusters per area, CN, and wax content,
WC) in five traditional cultivars and 13 newer clones. Data were collected over 4 years and alternate bearing was observed:
2001 and 2003 were years with high yields; in 2002 and 2004 yields were poor. The restricted maximum likelihood method was
used to calculate (co)variance components for analysis. Broad sense heritability was estimated and ranged from low (FN and
CN) to high (FW and WC) for the traits examined. There were positive genetic correlations between FW and WC and negative ones
between FN and FW throughout the 4 years. Genetic correlations between CN and the other traits were positive in good crop
years and negative in bad crop years. For each assessed clone, the four traits in the 4 years were evaluated using best linear
unbiased predictors (BLUP) of the clonal breeding values. The BLUP scores for both FW and WC exhibited positive correlations
between pairs of years, whereas there were positive BLUP correlations for CN when the high yield and low yield years were
examined separately. The traditional cultivars and the newer clones were also characterized. Some of the clones were better
than the traditional cultivars with regard to wax yield and reliability of production. The trait characteristics and the future
breeding are discussed. 相似文献
75.
Taketo OBITSU Mitsuru KAMIYA Yuko KAMIYA Masahito TANAKA Toshihisa SUGINO Kohzo TANIGUCHI 《Animal Science Journal》2011,82(4):531-536
Effects of exposure to hot environment on urea metabolism were studied in lactating Holstein cows. Four cows were fed ad libitum a total mixed ration and housed in a temperature‐controlled chamber at constant moderate (18°C) or high (28°C) ambient temperatures in a cross‐over design. Urea nitrogen (N) kinetics was measured by determining urea isotopomer in urine after single injection of [15N2]urea into the jugular vein. Both dry matter intake and milk yield were decreased under high ambient temperature. Intakes of total N and digestible N were decreased under high ambient temperature but urinary urea‐N excretion was increased. The ratio of urea‐N production to digestible N was increased, whereas the proportion of gut urea‐N entry to urea‐N production tended to be decreased under high ambient temperature. Neither return to the ornithine cycle, anabolic use nor fecal excretion of urea‐N recycled to the gut was affected by ambient temperature. Under high ambient temperature, renal clearance of plasma urea was not affected but the gut clearance was decreased. Increase of urea‐N production and reduction of gut urea‐N entry, in relative terms, were associated with increased urinary urea‐N excretion of lactating dairy cows in higher thermal environments. 相似文献
76.
K Mutoh H Wakuri K Taniguchi 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1999,61(5):493-496
The intercalated duct cells were observed in the A and B islets of the chicken pancreas. These cells adhered with each other by intercellular junctional complexes at the apical side. They had many microvilli projecting into the lumen. Abluminally, they displayed extended slender cytoplasmic processes between islet endocrine cells. Administration of alloxan resulted to denser cytoplasm and a more prominent thickening of cytoplasmic processes of the intercalated duct cells, although the blood glucose levels did not show appreciable changes by the treatment. The intercalated duct epithelial cells appeared clearly as stellate cells. The lysosomes increased in size and number with passage of time after alloxan administration. The present findings may suggest that intercalated ducts are not only anatomically important as a structure passing through the islet but also play physiologically by protecting the islet endocrine cells. 相似文献
77.
We developed a quantitative PCR assay for detecting the parasitic ciliate Cryptocaryon irritans, which causes “white spot disease” in marine fishes, from the natural environment. A specific primer set for C. irritans was designed and its high specificity was confirmed in silico: almost all of the sequences deposited in the GenBank nucleotide
database were covered, 22/23 for the forward primer and 7/7 for the reverse primer. We estimated that there were 3,415.9 rRNA
gene copies per genome of C. irritans. In artificial mixture experiments to validate whether the qPCR assay is applicable to natural samples, the estimated copy
numbers showed significantly positive correlations with the number of theronts added (p < 0.001). When we applied this qPCR assay to natural samples collected bimonthly from surface and bottom seawaters at an
aquaculture site (water depth, 10 m) from May 2009 to March 2010, we only detected C. irritans (112.0 ± 6.3 cells/l) in the surface seawater sample in November. This qPCR assay is a useful tool for detecting C. irritans rapidly and quantitatively in natural environments; it could also help advance our understanding of the ecology of C. irritans, as well as facilitate the diagnosis of the disease. 相似文献
78.
Mizuta Shoshi Asano Chisato Yokoyama Yoshihiro Taniguchi Motoki 《Fisheries Science》2012,78(2):399-406
The molecular species of collagen in the muscular and vertebral parts of white sturgeon Acipenser transmontanus were examined by biochemical techniques. Pepsin-solubilized collagens were prepared from these parts, and fractionated into
major and minor collagen fractions by differential salt precipitation at acidic or neutral pH. Collagens contained in these
fractions showed several features characteristic of fish type I and V collagens, respectively, in SDS-PAGE patterns and the
amino acid compositions of both parts. These results suggest that at least two molecular species, corresponding to type I
and V collagens, are distributed in the muscular and vertebral parts of white sturgeon. 相似文献
79.
Kelly Vargas Yoshiho Asakura Minoru Ikeda Nobuhiko Taniguchi Yasuhiro Obata Katsuyuki Hamasaki Kotaro Tsuchiya Shuichi Kitada 《Fisheries Science》2008,74(3):533-543
ABSTRACT: Allozyme variation of the littleneck clam Ruditapes philippinarum was evaluated in four samples from Nameishi and Matsuo in the Ariake Sea, Ryugatake and Ushibuka in the Shiranui Sea off Kyushu Island, Japan, and in one sample from Jinzhou, China, in the Bohai Sea. A Ruditapes bruguieri sample imported from the Korean Bay off Nampo, North Korea was also studied. Among the R. philippinarum samples, heterozygosity varied from 0.265 to 0.301 and F IS estimates indicated significant homozygosity excess in 15 of 40 loci analyzed. Deviations from Hardy–Weinberg equilibrium were significant in all samples ( P < 0.05). Pairwise F ST estimates indicate that genetic differences between the Chinese and Japanese samples were very low, but significantly different from zero. Mixture proportions with 95% confidence intervals of Chinese R. philippinarum in Nameishi and Matsuo were estimated at 0.4098 [0.2512, 0.5705] and 0.4899 [0.3262, 0.6540], respectively. However, genetic invasion of stocked Chinese R. philippinarum into wild populations in the Ariake Sea remains uncertain due to the low precision of the estimates caused by the high similarity of allele frequencies between Jinzhou and the Ariake Sea. 相似文献
80.