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排序方式: 共有137条查询结果,搜索用时 15 毫秒
11.
The embedment tests of laminated veneer lumber (LVL) with two moduli of elasticity (MOE; 7.8 GPa and 9.8GPa), parallel strand lumber (PSL), and laminated strand lumber (LSL) were conducted in accordance with ASTM-D 5764. The load-embedment relation for each of these engineered wood products (EWPs) was established. The directional characteristics of bearing strength (e), initial stiffness (k
e), and effective elastic foundation depth were obtained from the tested results. The effective elastic foundation depth (=E/k
e,E = MOE), based on the theory of a beam on elastic foundation, was obtained from thek
e and MOE. An of 90° (perpendicular to the grain) was calculated by dividingE
90 [MOE of 90° from the compression test, but MOE of 0° (E
0), parallel to the grain, obtained from the bending test] byk
e90, the initial stiffness of 90°. This study aimed to obtain the bearing characteristics of each EWP, taking into consideration their anisotropic structures, for estimating the fastening strength of a dowel-type fastener. The relations between the bearing coefficients (
e,k
e,) on the loading direction and dowel diameter were established from the load-embedment curves. Based on the results of the embedment test, tested EWPs showed different tendencies in all directions from wood and glued laminated timber.Part of this study was presented at the 49th Annual Meeting of the Japan Wood Research Society, Tokyo, April 1999 相似文献
12.
Kohei MURAKAMI Tomohiro YONEZAWA Naoaki MATSUKI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(12):1715-1717
Idiopathic polyarthritis (IPA) is a very common inflammatory arthropathy in the dog.
Canine IPA is diagnosed mainly by detecting increased number of leukocytes in the synovial
fluid (SF), which is easily influenced by glucocorticoid therapy. We obtained 31 SF
samples from 24 IPA dogs prior to (n=19) and/or after
(n=12) 1 to 10 weeks of glucocorticoid therapy. The SF total protein
concentrations of IPA dogs were significantly higher than those of dogs with non-arthritis
diseases (n=34) and healthy controls (n=10). Our data
revealed that the SF total protein concentrations are not influenced by several weeks of
glucocorticoid therapy. Hence, the SF total protein concentration is applicable as a
diagnostic marker of canine IPA even when the patients are receiving glucocorticoid
therapy. 相似文献
13.
Takafumi SUZUKI Naohito NISHII Satoshi TAKASHIMA Tatsuya MATSUBARA Atsushi IWASAWA Hirofumi TAKEUCHI Kohei TAHARA Tatsuyuki HACHISU Hitoshi KITAGAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1379-1383
Polyclonal immunoglobulin (Ig) G autoantibodies against insulin have been
identified in sera of healthy cats. We purified and fractionated insulin-binding IgGs from
cat sera by affinity chromatography and analyzed affinity of insulin-binding IgGs for
insulin and their epitopes. Following the passing of fraction A, which did not bind to
insulin, insulin-binding IgGs were eluted into two fractions, B and C, by affinity
chromatography using a column fixed with bovine insulin. Dissociation constant (KD) values
between insulin-binding IgGs and insulin, determined by surface plasmon resonance analysis
(Biacore™system), were 1.64e−4 M for fraction B (low affinity IgGs) and
2e−5 M for fraction C (high affinity IgGs). Epitope analysis was conducted
using 16 peptide fragments synthesized in concord with the amino acid sequence of feline
insulin by an enzyme-linked immunosorbent assay. Fractions B and C showed higher
absorbance (affinity) of the peptide fragment of 10 amino acid residues at the
carboxyl-terminal of the B chain (peptide No. 19), followed by peptide fragments of 6 to
15 amino acid residues of the B chain (peptide No. 8). Fraction C showed a higher
absorbance to 7 to 16 amino acid residues of the B chain (peptide No. 5) compared with the
absorbance of fraction B. Polyclonal insulin-binding IgGs may form a macromolecule complex
with insulin through the multiple affinity sites of IgG molecules. Feline insulin-binding
IgGs are multifocal and may be composed of multiple IgG components and insulin. 相似文献
14.
Mao Mori Kohei Mizobata Taro Ichii Philippe Ziegler Takehiro Okuda 《Fisheries Oceanography》2022,31(1):19-39
The Antarctic toothfish (Dissostichus mawsoni) is an important fishery species widely distributed in the Southern Ocean, especially in areas covered by sea ice. Understanding fish distributions and life cycles, including the transport and survival of eggs and larvae, is essential for the assessment and sustainable management of the fishery. However, owing to difficulties with in situ winter observations, information on the early life stages of D. mawsoni is lacking. Here, we investigated the transport pathways of fish eggs and larvae through a particle tracking study, using satellite-derived ocean surface velocities in the East Antarctic region, which includes important D. mawsoni habitats and exploratory fisheries. Our results indicate that particles released from continental slopes are more likely to be successfully transported to suitable settlement grounds than those released from the BANZARE Bank (the southern region of the Kerguelen Plateau), which is situated further north and has been hypothesized to be a potential spawning ground for D. mawsoni. This study demonstrates successful source–settlement connections in relation to ocean recirculation and suggests important settlement regions for D. mawsoni larvae in the East Antarctic region. 相似文献
15.
Tetsuro?Shiraishi Kohei?Ohta Akihiko?Yamaguchi Mari?Yoda Hisashi?Chuda Michiya?MatsuyamaEmail author 《Fisheries Science》2005,71(3):531-542
ABSTRACT: Final oocyte maturation and ovulation of captive chub mackerel Scomber japonicus with fully yolk-accumulated oocytes were induced by a single injection of human chorionic gonadotropin. Reproductive parameters, including spawning frequency and batch fecundity, which are required to estimate spawning biomass in pelagic fish by the daily egg production method, were analyzed. Germinal vesicle migration (GVM) occurred at 18–24 h post-injection, and the hydration and ovulation of oocytes were completed at 30 and 36 h post-injection, respectively. The results of the maturation process suggest that fish with GVM-stage ovaries captured in the daytime from the field are capable of spawning on the night following their capture. The oocytes used in the oocyte size-frequency distribution method for batch fecundity estimates should be at late GVM and more advanced stages. The results of sequential artificial insemination showed that the quality of ovulated eggs held in the ovarian lumen rapidly deteriorated as time progressed after ovulation. This indicates that the fertilization window for the ovulated eggs of chub mackerel lasts only a few hours, and spawning behavior should be performed within a few hours after ovulation in the wild population. 相似文献
16.
Akira Inoue Kohei Takadono Ryuji Nishiyama Kenji Tajima Takanori Kobayashi Takao Ojima 《Marine drugs》2014,12(8):4693-4712
A major alginate lyase, FlAlyA, was purified from the periplasmic fraction of an alginate-assimilating bacterium, Flavobacterium sp. strain UMI-01. FlAlyA showed a single band of ~30 kDa on SDS-PAGE and exhibited the optimal temperature and pH at 55 °C and pH 7.7, respectively. Analyses for substrate preference and reaction products indicated that FlAlyA was an endolytic poly(mannuronate) lyase (EC 4.2.2.3). A gene fragment encoding the amino-acid sequence of 288 residues for FlAlyA was amplified by inverse PCR. The N-terminal region of 21 residues except for the initiation Met in the deduced sequence was predicted as the signal peptide and the following region of six residues was regarded as propeptide, while the C-terminal region of 260 residues was regarded as the polysaccharide-lyase-family-7-type catalytic domain. The entire coding region for FlAlyA was subjected to the pCold I—Escherichia coli BL21(DE3) expression system and ~eight times higher yield of recombinant FlAlyA (recFlAlyA) than that of native FlAlyA was achieved. The recFlAlyA recovered in the periplasmic fraction of E. coli had lost the signal peptide region along with the N-terminal 3 residues of propeptide region. This suggested that the signal peptide of FlAlyA could function in part in E. coli. 相似文献
17.
Asagiri M Hirai T Kunigami T Kamano S Gober HJ Okamoto K Nishikawa K Latz E Golenbock DT Aoki K Ohya K Imai Y Morishita Y Miyazono K Kato S Saftig P Takayanagi H 《Science (New York, N.Y.)》2008,319(5863):624-627
Cathepsin K was originally identified as an osteoclast-specific lysosomal protease, the inhibitor of which has been considered might have therapeutic potential. We show that inhibition of cathepsin K could potently suppress autoimmune inflammation of the joints as well as osteoclastic bone resorption in autoimmune arthritis. Furthermore, cathepsin K-/- mice were resistant to experimental autoimmune encephalomyelitis. Pharmacological inhibition or targeted disruption of cathepsin K resulted in defective Toll-like receptor 9 signaling in dendritic cells in response to unmethylated CpG DNA, which in turn led to attenuated induction of T helper 17 cells, without affecting the antigen-presenting ability of dendritic cells. These results suggest that cathepsin K plays an important role in the immune system and may serve as a valid therapeutic target in autoimmune diseases. 相似文献
18.
Suzuki K Matsuo T Hashizume D Fueno H Tanaka K Tamao K 《Science (New York, N.Y.)》2011,331(6022):1306-1309
The cyclobutadiene (CBD) molecule C(4)H(4) deviates from a high-symmetry square geometry to compensate for its antiaromatic electronic structure. Here, we report a CBD silicon analog, Si(4)(EMind)(4) (1), stabilized by the bulky 1,1,7,7-tetraethyl-3,3,5,5-tetramethyl-s-hydrindacen-4-yl (EMind) groups, obtained as air- and moisture-sensitive orange crystals by the reduction of (EMind)SiBr(3) with three equivalents of lithium naphthalenide. X-ray crystallography reveals a planar and rhombic structure of the Si(4) four-membered ring, with alternating pyramidal and planar configurations at the silicon atoms. The large (29)Si chemical shift differences (Δδ > 350 parts per million) in the solid-state nuclear magnetic resonance spectra suggest a contribution of an alternately charge-separated structure. The rhombic-shaped charge-separated singlet state of compound 1 thus stabilizes its cyclic 4π-electron antiaromaticity in a manner that contrasts sharply with the bond-length alternation, characterizing the rectangular distortion of carbon-based CBD. 相似文献
19.
20.
Significance of tumor-infiltrating immune cells in spontaneous canine mammary gland tumor: 140 cases
Saeki K Endo Y Uchida K Nishimura R Sasaki N Nakagawa T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(2):227-230
The numbers of tumor infiltrating T lymphocytes, B lymphocytes and antigen presenting cells were evaluated in an immunohistochemical manner in 140 canine spontaneous mammary gland tumor (MGT) tissues. As a result, we found a statistically significant increase in the number of intratumoral T lymphocytes (23.2 ± 23.8) in the malignant MGT group (n=51) compared with the benign MGT group (14.0 ± 16.0, n=89; P<0.05). Moreover, the high T lymphocyte infiltration in the malignant group correlated with poor prognosis in multivariate analysis (P<0.05). This study indicated the relationship between increased infiltrating T lymphocytes and canine MGT malignancy. 相似文献