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61.
Kensuke ARAKAWA Yasushi KAWAI Kenji FUJITANI Junko NISHIMURA Haruki KITAZAWA Ken-ichi KOMINE Kenzo KAI Tadao SAITO 《Animal Science Journal》2008,79(5):634-640
The use of bacteriocins from Lactobacillus gasseri , a probiotic lactic acid bacterium, as bio-preservatives in the food industry and animal formulations has been limited because few strains of Lb. gasseri are cultivated and produce a bacteriocin in natural media such as milk and milk-based media. By the determination of the growth-supplements to milk among the 47 nutrients, Lb. gasseri JCM1131T , LA39 and LA158 isolated from human feces were successfully cultured in reconstituted skim milk and cheese whey using proteose peptone as a nutrient supplement, where Lb. gasseri LA39 produced a useful bacteriocin, gassericin A, with effective growth-inhibiting activity against Gram-positive food-borne pathogens. The data suggest these developed low-cost safe media supporting enough production of bacteriocins by the probiotic Lb. gasseri LA39 could be used to improve the safe bio-preservation of foods and therapy of bovine mastitis, and extra cheese whey produced by cheese making industry is reused in the cultivation for probiotics effectively. 相似文献
62.
Takuya Tetsumura Yasuyo Matsumoto Makiko Sato Chitose Honsho Kensuke Yamashita Haruki Komatsu Yasuhiro Sugimoto Hisato Kunitake 《Scientia Horticulturae》2008
Murashige and Skoog medium (MS), woody plant medium (WPM), and a mixture of equal parts of MS and WPM (MW) were compared for in vitro shoot proliferation and rooting of four highbush blueberry (Vaccinium spp.) cultivars. During the multiplication stage, the shoots on WPM showed worse growth than the other shoots. The MW produced the best shoot growth. The shoots on MS grew well but tended toward hyperhydricity in the multiplication stage, especially in the case of ‘Bluecrop’ shoots. Rooting was worst in the shoots multiplied on WPM, while the best rooting percentage of ‘Bluecrop’ shoots was obtained on MS, and that of ‘O’Neal’ on MW. The rooted shoots developed a good root system and were easily acclimatized after potting. 相似文献
63.
Yasutomo Hori Masayuki Iguchi Yasuhiro Heishima Yohei Yamashita Kensuke Nakamura Atsushi Hirakawa Akihito Kitade Toshiki Ibaragi Michio Katagi Tamotsu Sawada Masashi Yuki Nobuyuki Kanno Haruki Inaba Noriko Isayama Hideyuki Onodera Naoki Iwasa Mikio Kino Mikihiro Narukawa Syuhei Uchida 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2018,32(3):922-929
64.
Salmonella typhimurium infection occurred in a children's zoo where 11 fowl and 85 mammals were kept. Initially, the guinea pigs were infected and transmitted the infection to the fowl and rabbits. These mammals responded to medication and cleared of the infection; however, the birds were judged to contain excreters despite four regimens of treatment with antibiotics. Cloacal swabs were taken from all the birds. One turkey was positive for Salmonella and was destroyed. Pooled fecal samples from the birds were again positive. All the birds were tested serologically, and two birds, a goose and a turkey, were positive with Salmonella pullorum-gallinarum antigen, which was assumed to be a cross reaction with S. typhimurium antigen. The two birds were destroyed and the goose yielded Salmonella. The infection was finally eradicated, and the serologic examination was considered to be the most useful procedure for detection of the excreters. 相似文献
65.
Teruhiko Nitoda Haruki Kurumatani Hiroshi Kanzaki Kazuyoshi Kawazu 《Pest management science》1999,55(5):563-565
A previously reported bioassay method for Spodoptera litura chitinase inhibitors has been improved by use of colloidal chitin powder with a uniform particle size. This improvement made the assay four times more sensitive. Detection of three active supernatants by screening of supernatants and cell extracts from 135 fermentation broths has proved the efficiency of this improved method. © 1999 Society of Chemical Industry 相似文献
66.
Steven D. Tanksley Dario Bernachi Teresa Beck-Bunn Davy Emmatty Yuval Eshed Shuji Inai Joachin Lopez Vincent Petiard Haruki Sayama John Uhlig Daniel Zamir 《Euphytica》1998,99(2):77-83
A pair of processing tomato (Lycopersicon esculentum Mill.) lines, nearly isogenic for the Tm-2a gene for resistance to tobacco mosaic virus, were grown in replicated trials under commercial production conditions in five
locations worldwide. The lines were evaluated for 17 processing traits including fruit yield, size, soluble solids concentration,
color, firmness, and viscosity. Eight of those traits differed significantly among the nearly-isogenic lines (NILs). Most
notably, the NIL heterozygous for Tm-2a yielded, on average, 16% more than the NIL homozygous for the susceptible allele and 33% more than the NIL homozygous for
Tm-2a. Viscosity was lower in the heterozygous NIL and the homozygous and heterozygous resistant NILs had softer fruit with larger
stem scars compared to the homozygous susceptible NIL. These results indicate the presence of the Tm-2a locus affects many traits of importance for processing tomatoes and may be used, in the heterozygous state, to significantly
increase yield. Whether the observed effects are due to the Tm-2a gene itself or genes associated via linkage drag could not be determined.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
67.
Futoshi YAZAMA Haruki SATO Tomoko SONODA 《The Journal of reproduction and development》2015,61(5):399-406
An experimental ischemia (EI)-induced mouse model was used to
analyze pathological and biochemical alterations in testes. Initial
morphological changes were observed in Sertoli cells of EI testes at
the light microscopic level. Examination of the ultrastructure using
transmission electron microscopy confirmed that Sertoli cells were
partially detached from the basement membrane of the seminiferous
epithelium and that the cell membranes of adjacent Sertoli cells were
not joined. The functional integrity of the blood-testis barrier (BTB)
was assessed using the lanthanum tracer technique. Lanthanum had
penetrated into the spaces between adjacent Sertoli cells in the
adluminal compartment up to the lumen of the seminiferous epithelium
in EI testes. Proteome analysis showed that the expression of heat
shock protein (HSP) 70 was significantly upregulated in EI testes.
Western blot analysis confirmed that the expression of HSP70 increased
in a time-dependent manner after the EI procedure. HSP70
immunostaining was observed in spermatocytes and in round and
elongated spermatids in EI testes. Our results suggest that a change
in the junctions between adjacent Sertoli cells on the basal
compartment is involved in the BTB disruption in EI testes. Therefore,
male infertility caused by the BTB disruption could be associated with
heat stress induced by ischemia. 相似文献
68.
Surojanametakul V Doi H Shibata H Mizumura T Takahashi T Varanyanond W Wannapinpong S Shoji M Ito T Tamura H 《Journal of agricultural and food chemistry》2011,59(6):2131-2136
This study was designed to develop a novel sandwich enzyme-linked immunosorbent assay (ELISA) for the detection and quantification of coconut milk proteins in processed foods. The developed sandwich ELISA was able to detect coconut milk proteins from various coconut milk products and did not show any cross-reactivity with 41 of 42 kinds of popularly used food ingredients, thus reflecting great specificity for coconut milk proteins. In addition, the established ELISA is highly sensitive and allowed the detection of 0.31 μg/g of coconut milk protein in complex food matrices. This proposed assay could serve as a useful tool for the detection of the presence of hidden coconut milk proteins in processed foods. 相似文献
69.
Kumita Kohei Kitazawa Yugo Tokuda Ryosuke Miyazaki Akio Maejima Kensaku Namba Shigetou Yamaji Yasuyuki 《Journal of General Plant Pathology》2021,87(4):254-258
Journal of General Plant Pathology - In July 2018, tillandsia (Tillandsia sp.) plants with brown to dark brown lesions on the leaves and pseudobulbs were found in Japan. Based on morphological and... 相似文献
70.
Kiyoshi NAKAMURA Kensuke ARAKAWA Yasushi KAWAI Narimi YASUTA Takahiro CHUJO Masamichi WATANABE Hiroyuki IIOKA Masashi TANIOKA Junko NISHIMURA Haruki KITAZAWA Koichi TSURUMI Tadao SAITO 《Animal Science Journal》2013,84(2):144-149
Gassericin A (GA) is a circular bacteriocin produced by Lactobacillus gasseri LA39. In this study, GA‐containing concentrate was prepared using a cross‐flow membrane filtration device (30 kDa cut‐off) from the culture supernatant of Lb. gasseri LA39 cultivated in a cheese whey‐based food‐grade medium. The bacteriocin activity titer in the concentrate was 16 times as high as that of the culture supernatant and was completely maintained through each incubation at 4°C for 3 months, 37°C for 2 months, 60°C for 5 h, and 100°C for 30 min. The GA‐containing concentrate was used with glycine powder to make custard creams, and then four representative strains of custard cream spoilage bacteria (Bacillus cereus, Lactococcus lactis subsp. lactis, Achromobacter denitrificans and Pseudomonas fluorescens) were individually inoculated at c. 103 colony forming units/g in the custard creams. Throughout 30 days of incubation at 30°C, all of the inoculated bacteria were completely inhibited by the combination of 5% (w/w) of the GA‐containing concentrate and 0.5% (w/w) glycine. This is the first highly practical application of GA to foods as a biopreservative, and the concentration method and the bacteriocin concentrate would contribute to biopreservation of several foods. 相似文献