Prevalence of peste des petits ruminants among sheep and goats in India

This study measured the clinical prevalence of peste des petits ruminants (PPR) among sheep and goats in India between 2003 and 2009 by analyzing clinical samples from suspected cases of PPR that were submitted to the Rinderpest and Allied Disease Laboratory, Division of Virology, IVRI, Mukteswar for PPR diagnosis. PPR outbreaks were confirmed by detecting PPR virus (PPRV)-specific antigen in the clinical samples. Clinical samples (blood, nasal swabs, spleen, lymph node, kidney, liver, intestine, and pooled tissue materials) were taken from a total of 592 sheep and 912 goats in different states of India and screened for the presence of PPRV antigen using a monoclonal antibody-based sandwich ELISA kit. A total of 20, 38, and 11 laboratory-confirmed PPR outbreaks occurred among sheep, goat, and combined sheep and goat populations, respectively. Our findings provide evidence of widespread PPR endemicity in India. The underlying reasons could be variations in husbandry practices in different geographical regions, agro-climatic conditions, and livestock migration. Furthermore, decrease in the number of PPR outbreaks over time might be due to the effectiveness of current live PPR vaccines and timely vaccination of target species. Vaccination against PPR has been practiced in India since 2002 to control this disease.     

Molecular characterization of Blastocystis isolates from children and rhesus monkeys in Kathmandu, Nepal

To investigate the possible transmission of Blastocystis organisms between local rhesus monkeys and children in Kathmandu, Nepal, we compared the subtype (ST) and sequence of Blastocystis isolates from children with gastrointestinal symptoms and local rhesus monkeys. Twenty and 10 Blastocystis isolates were established from 82 and 10 fecal samples obtained from children and monkeys, respectively. Subtype analysis with seven sequence-tagged site (STS) primers indicated that the prevalence of Blastocystis sp. ST1, ST2 and ST3 was 20%, 20% and 60% in the child isolates, respectively. In contrast to human isolates, ST3 was not found in monkey isolates and the prevalence of ST1 and ST2 was 50% and 70%, respectively, including three mixed STs1 and 2 and one isolate not amplified by any STS primers, respectively. Since Blastocystis sp. ST2 has been reported as the most dominant genotype in the survey of Blastocystis infection among the various monkey species, sequence comparison of the 150 bp variable region of the small subunit rRNA (SSU rRNA) gene was conducted among ST2 isolates of humans and monkeys. Sequence alignment of 24 clones developed from ST2 isolates of 4 humans and 4 monkeys showed three distinct subgroups, defined as ST2A, ST2B and ST2C. These three subgroups were shared between the child and monkey isolates. These results suggest that the local rhesus monkeys are a possible source of Blastocystis sp. ST2 infection of humans in Kathmandu.     

Root aeration improves yield and water use efficiency of tomato in heavy clay and saline soils

Water logging and salinity of the soil alter both the physical and biological environment of plant roots. In two experiments, we investigated the effects of imposed aeration on yield and the physiological response of tomato (Lycopersicon esculentum L.) variety Improved Apollo growing under protected conditions over a range of salinities (the salinity experiment), and under constant field capacity (FC) or drier soil conditions (the moisture experiment). Subsurface irrigation with aerated water (12% air in water) stimulated above-ground growth, and enhanced the reproductive performance through earliness for flowering and fruiting compared with the control. Fruit yield of tomato with aeration in the moisture experiment was increased by 21% compared with the control (4.2 kg versus 3.7 kg per plant), and the effect of aeration on fruit yield was greater in FC than in the drier treatment. Fruit yield was increased by 38% in saline soil due to aeration compared with the non-aerated control. Increasing salinity from 2 to 8.8 dS m⁻¹, and 10 dS m⁻¹ reduced fruit yield by 18% and 62%, respectively, but 4 dS m⁻¹ did not suppress yield. Aeration in both the experiments increased plant water use and water use efficiency (WUE), expressed as weight per unit of applied water. Biomass WUE was greater by 16% and 32% in the moisture and salinity experiments, respectively. The increased yield with aeration was also accompanied by an increased harvest index (HI) defined as the proportion of dry fruit biomass to total dry biomass, greater mean fruit weight, high fruit DM, and increase in leaf chlorophyll content and shoot: root ratio, and a reduced water stress index (computed from the difference between air and leaf temperature). The benefit gained from aerating irrigation water was not only observed under conditions where air-filled porosity may be low (e.g., in poorly structure sodic soils, or at field capacity in clay soils), but also in drier soils.     

Assessment of suitability of two serotype A candidate vaccine strains for inclusion in FMD vaccine in India

The recent type A foot and mouth disease virus field isolates recovered in India are shown to be antigenically quite divergent from the in-use vaccine strain (IND 17/82), warranting the selection of a suitable vaccine strain which can cover this diversity in antigenic spectrum. In earlier studies employing neutralization test with anti-146S rabbit sera raised against eight candidate vaccine strains, IND 81/00 and IND 40/00 belonging to genotype VII were found to offer the best antigenic coverage. In order to assess the credibility of IND 81/00 and IND 40/00 as vaccine strains, 17 recent isolates received during 2005-2006 and representative isolates from older genotypes were subjected to two-dimensional micro-neutralization assay using bovine convalescent serum (against IND 81/00 and IND 40/00) and bovine vaccinate serum (against IND 40/00). From the results it is evident that both the isolates IND 81/00 (antigenic relationship 'r-value' >0.40 with 86% of isolates) and IND 40/00 ('r-value' >0.40 with 78% of isolates) show nearly equal antigenic relatedness with the recent field viruses and hence both of these are effective vaccine candidates in present context. Though very limited in its extent, these useful data obtained with antisera raised in homologous host system are logical extension of the on going quest for the appropriate vaccine strain and circumvents species disparities in the immune recognition of epitopes.     

Farmers’ willingness to pay for community integrated pest management training in Nepal

The concept of community integrated pest management (IPM), which is well developed in Indonesia and Vietnam, was recently introduced in Nepal. However, it has not been widely practiced, due mainly to lack of financial and technical support. This study determined an individual’s willingness to pay (WTP) for community IPM training. Determinants of WTP were identified; and sample average estimates, opportunity costs of training, and probability values were used to estimate WTP for a group of households. Estimated WTP revealed that individuals were in favor of community IPM, hence it could be implemented with the support of local villagers. Community IPM demand functions showed that individuals’ knowledge and awareness of pesticide pollution are crucial for implementation. The annual welfare gained by providing five days community IPM training was calculated to be US $25.23 per household. Kishor Atreya has a Master’s degree in Environmental Science and is currently working at Alternative Development and Research Center (ADRC) as Research Director. ADRC – Nepal is a national non-governmental organization dedicated to interdisciplinary research for alternative development. His research interests are in development of agri-environmental indicators, land and water quality, land use change and degradation, agricultural intensification and environment, and economic valuation of environmental pollution.     

Growth and yield responses of a Nepalese spring wheat cultivar to the inoculation with Nepalese Azospirillum spp at various levels of nitrogen fertilization

The Azospirillum 10SW used in our experiments was isolated from roots of wheat growing in nitrogen-poor soil of a hilly region of Nepal, where inorganic nitrogen fertilizers were never used. The main objectives of this work were to assess the effects of inorganic nitrogen fertilization in the yield responses of wheat grown in association with the bacteria. The in vitro experiments were done in laboratory, whereas the pot experiments were performed in a greenhouse. The nitrogenase activities of in vitro grown Azospirillum were repressed by nitrate. The magnitude of repression was lower when the bacteria were growing in association with wheat. The number of roots per plant was increased significantly in inoculated plants irrespective of the nitrate concentration of the medium. Inoculation with Azospirillum 10SW also increased the yield of wheat grown in pots with medium levels of nitrogen fertilization. These data show the possibility of inoculation of this Azospirillum spp. in combination with nitrogen fertilizer to improve the yield of wheat. Azospirillum inoculation enhanced the development of roots and shoots in the early growth stages of wheat. It may be one of the factors responsible for the yield increases. Received: 11 December 1996     

Gharials (Gavialis gangeticus) in Bardiya National Park,Nepal: Population,habitat and threats

1. The Critically Endangered gharial (Gavialis gangeticus) residing within Bardiya National Park (BNP) in Nepal constitutes the sixth major sub-population of this unique lineage; however, this population is not well studied. A 46 km protected stretch of the Babai River and a 60 km stretch of the Karnali River in and around BNP were surveyed for gharials in early 2017 and in 2019.
2. Gharial counts in 2017 – 17 from the Babai and one from the Karnali rivers – consisted of 10 adults, five sub-adults and three juveniles. In 2019, 19 gharials were counted – 18 for the Babai and one for the Karnali – comprising 11 adults, six sub-adults and two juveniles. In the Babai River within BNP, four breeding groups were identified. Gharials in BNP prefer sandy vs. rocky banks for basking and completely avoid sand-grass and clay banks. Habitat preference did not vary with size class.
3. Protected stretches of both rivers inside BNP (76 km) have resident gharial, intact habitats and few human threats. In contrast, the unprotected stretch of the Karnali (30 km) is threatened by boulder quarrying, sand mining and unlicensed fishing, and is avoided by gharials despite the availability of habitat.
4. This study established baseline data, including indices of population size, distribution and habitat preferences, and documented resident gharials that are breeding in the Babai River in BNP. The conservation of this Babai population is crucial for the species’ survival in BNP.
5. Based on this study, we recommend the following conservation actions on the Babai stretch: (a) determine hatching success; (b) assess juvenile recruitment; (c) consider supplementation by releasing captives; (d) study riverine features important as baselines; (e) accommodate upstream movements on the protected Babai stretch; and (f) protect the downstream Babai outside the boundary of the BNP.

     

Applications of reduced tillage in hills of central Nepal

Conventional tillage practices on steep and fragile landscape of Himalayan hills result in significant loss of topsoil during rainy season. Soil erosion in Nepal mid-hills is the most critical during pre-monsoon season. Many reviews argue that reduced tillage could be an option to tackle this problem. However, very few field experiments to evaluate reduced tillage systems have to date been conducted in this region. Thus, a field experiment was initiated in factorial randomized complete block design on acidic sandy loam soil (Lithic Dystochrept) during the summer season of 2001 at Kathmandu University (1500 masl) to assess the effects of tillage and cropping patterns on soil and nutrient losses, crop yield and soil fertility. Two main treatments viz. conventional and reduced till, and two sub-treatments viz. sole maize (Zea mays) and maize + soybean (Glycine max) were considered. Soil organic carbon (OC), total nitrogen (N), plant available phosphorus (P) and exchangeable potassium (K) were determined for the original soil and eroded sediment using standard methods. Two years of data indicated annual soil and nutrient losses to be significantly lowered by reduced till as compared to conventional till. Total annual soil loss from conventional and reduced till was 16.6 and 11.1 Mg/ha, respectively. Similarly, annual nutrient losses associated with the eroded sediment were 188 kg OC/ha, 18.8 kg N/ha, <1 kg P/ha and 3.8 kg K/ha for conventional till and 126 kg OC/ha, 11.8 kg N/ha, <1 kg P/ha and 2.4 kg K/ha for reduced till. Soil OC and N losses were significantly higher in conventional till and this may be one of the major causes of fertility depletion in the Nepalese hills. Soil chemical properties did not differ due to tillage and cropping systems; however, over years pH, N and P were increased irrespective of treatments. Although treatments were at par for maize grain yield, conventional till + soybean produced highest grain yield (4.0 Mg/ha) followed by reduced till + soybean (3.9 Mg/ha) and conventional till sole maize (3.8 Mg/ha). Mixed cropping of legumes and maize do not help conserve soil and nutrient loss in hills of central Nepal. Thus, reduced till could be a viable option for minimizing soil and nutrient losses without sacrificing economic yields in central hills of Nepal.     

On-farm measurements of nitrogen fixation by winter and summer legumes in the Hill and Terai regions of Nepal

Results are reported from on-farm surveys of N₂ fixation in Nepal, conducted between 1994 and 1999, involving the summer legumes soybean, mashbean and groundnut, and the winter legumes lentil and chickpea, at various locations in the Hill and Terai regions of Nepal. Additional less-detailed data were also collected for pigeonpea, grasspea and fababean. There were a total of 107 crops in the major and minor surveys. Estimates of the proportion of legume nitrogen (N) derived from N₂ fixation (Pfix) were determined using the natural ¹⁵N abundance method. Mean estimates of Pfix for the various species were 62% (soybean), 47% (mashbean), 57% (groundnut), 78% (lentil), 79% (chickpea), 75% (pigeonpea), 87% (grasspea) and 85% (fababean). Estimates of total N fixed (including roots) were 59 kg N ha⁻¹ (soybean), 28 kg N ha⁻¹ (mashbean), 153 kg N ha⁻¹ (groundnut), 72 kg N ha⁻¹ (lentil), 84 kg N ha⁻¹ (chickpea), 412 N ha⁻¹ (pigeonpea) and 80 N ha⁻¹ (grasspea and fababean). The on-farm measurements of N₂ fixation were generally similar to those of experimental crops in the same areas of the country. Correlation matrices of soil fertility parameters, shoot dry matter and N, and N₂ fixation revealed that the total amounts of N fixed were influenced primarily by crop growth. Based on the above figures, it was estimated that approximately 30,000 t N were fixed annually in Nepal by legumes, valued at US$ 15 million.