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61.
Kimura S Sasase T Ohta T Sato E Matsushita M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(1):103-105
The Spontaneously Diabetic Torii-Lepr(fa) (SDT-fa/fa) rat, a model of obese type 2 diabetes, shows obesity, hyperglycaemia and low bone mineral density (BMD). The objective of this study is to evaluate the effects of parathyroid hormone (1-34) [PTH(1-34)] on BMD and glucose metabolism in the SDT-fa/fa rat. SDT-fa/fa rats showed obesity with hyperglycaemia and decreased serum osteocalcin levels and the tibial BMD. A 4-week treatment of PTH(1-34) (20 μg/kg/day) increased the serum osteocalcin levels and the tibial BMD, and decreased the serum glucose levels without changing the serum insulin levels. These findings indicate that PTH(1-34) improved not only BMD but also glucose metabolism in SDT-fa/fa rats. This study suggests that PTH(1-34) is a novel agent for the treatment of diabetic osteoporosis. 相似文献
62.
Guanghua Wang Jun Murase Susumu Asakawa Makoto Kimura 《Biology and Fertility of Soils》2010,46(2):93-102
In order to evaluate the genetic diversity of cyanophage communities of rice fields, viral capsid assembly protein gene (g20) was amplified with primers CPS1 and CPS8. The DNA was extracted three times from viral concentrates obtained from floodwater
samples collected in each of four different plots (no fertilizer; P and K chemical fertilizers; N, P, and K chemical fertilizers;
and chemical fertilizers with compost). Denaturing gradient gel electrophoresis (DGGE) gave different g20 clones. The sequencing of DGGE bands revealed that the g20 genes of the floodwater were divergent and that the majority of clones formed several unique groups. However, they were more
closely related to g20 sequences from freshwaters than to those from marine waters, suggesting that g20 genes in terrestrial aquatic environments are different from those in marine environments. 相似文献
63.
K Kaneko E Uematsu Y Takahashi B Tong S Takino Y Wajiki T Kimura H Yamashiro Y Kaneko H Iwaisaki T Sugiyama T Yamada S Yamagishi 《Reproduction in domestic animals》2013,48(6):1001-1005
This study aimed to develop a polymerase chain reaction (PCR)‐based sexing and effective semen collection methods for black‐headed and straw‐necked ibis species. However, most birds are not sexually dimorphic, that is, the sexes appear similar. Therefore, the gender should be determined before semen collection. DNA was extracted from the blood samples of 11 black‐headed and 4 straw‐necked ibis. The sex was determined after PCR amplification of the EE0.6 region of W‐chromosome. The PCR products were separated using gel electrophoresis. A single band indicated the presence of the EE0.6 region and that the individual was a female, while no band indicated that the individual was a male. Further, the single bands from seven specimens were amplified. Semen collection was performed by massage or a combination of massage with electro‐ejaculation and was attempted during all four seasons. The semen was successfully collected in March from male straw‐necked ibis using the massage method. Limited motility, viability and concentration of straw‐necked ibis sperm were observed. The sperm length was 180 μm and that of the nucleus was 30 μm with acrosome located at the tip of the nucleus. Thus, the PCR‐based sexing proved to be an accurate molecular sexing method for black‐headed and straw‐necked ibis. Furthermore, we successfully collected semen and observed the stained sperm nucleus and acrosome of the straw‐necked ibis sperm. We propose that the use of this PCR methodology can be applied as a routine method for sex determination and semen collection in ibis species for future ecological research. However, considering our limited success, further studies on semen collection method are required. 相似文献
64.
Masashi?Hatamoto Takanori?Tanahashi Jun?Murase Kazuo?Matsuya Motoki?Hayashi Makoto?Kimura Susumu?AsakawaEmail author 《Biology and Fertility of Soils》2008,44(3):527-532
To estimate the succession and phylogenetic composition of the eukaryotic communities responsible for the decomposition of
rice straw compost under flooded conditions during the cultivation period of paddy rice, denaturing gradient gel electrophoresis
(DGGE) analysis targeting 18S rDNA followed by sequencing was conducted in a Japanese paddy field. The eukaryotic communities
in rice straw compost incorporated into the flooded paddy field were influenced by the mid-season drainage and mainly composed
of fungi (Ascomycota, Zygomycota, and Chytridiomycota) and protozoa (Ciliophora, Euglyphida, and Dactylopodida), most of which
existed continuously during the cultivation period of paddy rice. The results indicated that these eukaryotic members were
associated with the decomposition of rice straw compost in paddy field soil directly or indirectly. 相似文献
65.
Hypocretin/orexin is produced exclusively in the dorsal and lateral hypothalamus but its projection is widespread within the brain and plays important roles. In this paper, we review the independent discoveries of the hypocretin/orexin peptides, the neuroanatomy of this system, and the link to the sleep disorder narcolepsy that has led to the idea that this system plays a crucial role in the regulation of sleep and wakefulness. 相似文献
66.
The natural frequencies, aerodynamic noise, whistling noise, and self-excited vibration between the common circular saw with uniform thickness and the circular saw with step thickness (thin on its outer part and thick on its inner part) were researched during idling. The natural frequencies of the circular saw with step thickness increased with an increase in the thickness and in the radius of the thick part. The frequencies of the circular saws with step thickness (thick at the inner part) were higher than that of the thin circular saw with a uniform thickness of 0.5mm. The aerodynamic sound pressure level induced from circular saw teeth increased with an increase in the tooth thickness. The whistling noise and self-excited vibration easily occurred in the thick circular saws (1.5, 2.0, and 2.5mm). The whistling sound pressure level and the vibration amplitude increased with an increase in the thickness of the saw. None of the designed circular saws with step thickness generated whistling noises or self-excited vibration during idling.Part of this report was presented at the 45th annual meeting of the Japan Wood Research Society at Tokyo, April 1995 and the 12th International Wood Machining Seminar at Kyoto, October 1995 相似文献
67.
JE Lupton ET Baker N Garfield GJ Massoth RA Feely JP Cowen RR Greene TA Rago 《Science (New York, N.Y.)》1998,280(5366):1052-1055
The migration and evolution of a deep ocean hydrothermal event plume were tracked with a neutrally buoyant RAFOS float. The float remained entrained in the plume for 60 days, and the plume vorticity was calculated directly from the anticyclonic motion of the float. Concentrations of suspended particles, particulate iron, and dissolved manganese in the plume did not decay significantly during the 60 days, which indicates that event plumes would be easily detectable a year after formation. 相似文献
68.
M Sasaki Y Hayashi H Koie Y Yamaya J Kimura D Manglai S Kawashima H Endo M Yamamoto 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1999,61(9):1019-1022
The domestic horse (Equus caballus) have the large symmetrical guttural pouches (the auditory tube diverticulum) formed by saccate bulge of the auditory tube. In this study, CT examination was carried out in the head of Przewalski's horse (Equus przewalskii), the only true wild horse living at present. As results of the examination, Przewalski's horse possessed the large symmetrical guttural pouches divided into medial and lateral compartments by the stylohyoid bone. Moreover, the right and left guttural pouches meet each other at the median part to form a thin septum. As CT sections get close to the part of the occipital condyle, the lateral compartment disappeared, and the medial compartment gradually became small toward the base of the skull. These results indicate that the nuchal-basal part of the medial compartment is not well-developed as compared with the domestic horse. 相似文献
69.
70.
Makoto Kimura 《Soil Science and Plant Nutrition》2005,51(2):159-181
As rice fields are managed for improved rice production, many studies on aquatic organisms in the floodwater have been restricted to agronomically important members such as blue-green algae (BGA) and Azolla , and ecological studies on common aquatic organisms were relatively limited to specific group members. In the present paper, studies on all the aquatic organisms in the floodwater, from protozoa to insects and fishes, were reviewed worldwide from the viewpoint of rice field ecology, paying attention to their populations, seasonal variations, antagonistic relations and biomass, and the effect of field management. Most studies conducted in India and in the Southeast Asian countries dealt with the BGA and green algal populations. In contrast, protozoa and aquatic animals were extensively studied in rice fields in Europe. Japanese studies in the past were intermediate between the previously cited studies. They generally described specific groups depending on the authors' interests, and surprisingly few studies dealt with the community level and prey-predator relationships. Field management such as fertilizer and pesticide application, water management and plowing was found to play a major role in determining the populations of aquatic organisms. Overview of research on aquatic organisms in the floodwater of rice fields suggests several directions for future studies. 相似文献