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101.
Kim J Choi C Cho WS Chae C 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2000,62(7):771-773
Detection method of Lawsonia intracellularis was studied in formalin-fixed paraffin-embedded intestinal tissues from 5 naturally infected pigs by immunohistochemistry with a monoclonal antibody against outer membrane protein of L. intracellularis. Warthin-Starry silver stain revealed clusters of argyrophilic, slightly curved rod-shaped organisms in the apical cytoplasm of enterocytes. Immunohistochemical staining with a L. intracellularis-specific monoclonal antibody confirmed the presence of the organism in the apical cytoplasm of hyperplastic enterocytes. The presence of L. intracellularis in the ileum of pig with proliferative enteropathy was confirmed by polymerase chain reaction (PCR) further on the basis of amplification of 319 base pair products specific for porcine L. intracellularis chromosomal DNA. Immunohistochemistry and PCR may be a complementary method to confirm the diagnosis of L. intracellularis infection in pigs. 相似文献
102.
Effects of chilled drinking water on the performance of lactating sows and their litters during high ambient temperatures under farm conditions 总被引:1,自引:0,他引:1
J.H. Jeon S.C. Yeon Y.H. Choi W. Min S. Kim P.J. Kim H.H. Chang 《Livestock Science》2006,105(1-3):86-93
Heat stress has detrimental effects on lactating sows and their litters, including decreased feed intake and milk production in sows and decreased weight gain in their offspring. It also increases respiration rate, rectal temperature, and weaning to estrus interval in sows. This study was conducted to investigate the effects of chilled water (CW) on the performance of lactating sows and their litters exposed to high ambient temperatures for 21 days. Ninety multiparous sows (Yorkshire × Landrace; parity range: 2 to 5) and their litters (Yorkshire × Landrace × Duroc) were divided into 3 blocks (30 each). Sows and their litters within each block were randomly assigned to one of the three treatment groups (n = 10 each). Each group was provided with drinking water at 10, 15, or 22 °C (control) under farm conditions where ambient temperature above 25 °C was consistent during the experimental period. Sows on CW (both 10 and 15 °C) consumed more feed and water than did control (P < 0.01) and higher estimated milk production compared with control (P < 0.01), but had lower rectal temperature and respiration rate compared with control (P < 0.01). Mean weaning weight (P < 0.01) and average daily gain (P < 0.01) of litters in CW groups were higher than those in control group. There were no differences in the measured variables, with the exception of the respiration rates, between the two CW treatment groups. These results suggest that CW may improve the performance of sows and their litters exposed to high ambient temperatures and the 15 °C water, from an energy viewpoint, may be more effective. 相似文献
103.
104.
Sang-Hyun Lee Jin-Ho Choi Wol-Soo Kim Tae-Ho Han Yong-Seo Park Hirosi Gemma 《Scientia Horticulturae》2006
This study was carried out to investigate the cause of stone cell formation in pear (Pyrus pyrifolia cv. ‘Niitaka’) flesh. Potted plants grown in a glass house were subjected to water stress conditions without irrigation for 30 days from 30 days before full bloom (BFB treatment), full bloom (FB treatment) and 30 days after full bloom (AFB treatment). Control plants were drip-irrigated daily maintaining a soil matrix potential around −40 ± 5 kPa. The formation of stone cells in pear flesh increased in the FB treatment and AFB treatment plants and this tendency was sustained until the harvest season. Root activity was investigated 60 days after full bloom (DAFB) and the triphenyltetrazolium chloride (TTC) reduction potential, the formazan content and leaf water potential were investigated 30, 45, and 60 DAFB. Root activity decreased progressively due to the effect of water stress. Also, the Ca content in leaf and flesh was lower. The peroxidase activity was high in the flesh at the early stages of fruit growth and decreased at the late stages of fruit growth, and then a higher increase of peroxidase activity was observed in water-stressed fruit. The reduction in calcium content of leaf and fruit in plants under water stress may be related to the reduction of root activity and leaf water potential. The increase in peroxidase activity under water stress may be due to limited calcium absorption. Higher peroxidase activity may induce the accumulation of lignin in the cell wall and promote the formation of stone cells in pear flesh. We conclude that water stress condition during the early stages of fruit growth is one of several factors that determine the formation of stone cells in pear flesh. 相似文献
105.
In the skull of the Korean native goat, the parietal region was classified into four types by the degree of the fusion of the bones, the os interparietale, the os parietale and the squama occipitalis of the os occipitale, and the structural variations of these fusions. The fusion appeared first in the sutura inter-parietoparietalis and that of the sutura sagittalis of both ossa parietalia was followed. There was no fusion between the os parietale and the squama occipitalis of the os occipitale. These results suggest that the os interparietale developed independently but fused to the os parietale after birth, and the os parietale were developed as paired bones in prenatal life and then fused together according to age. 相似文献
106.
D. U. Han C. Choi J. Kim W.‐S. Cho H.‐K. Chung S.‐K. Ha K. Jung C. Chae 《Zoonoses and public health》2002,49(7):346-348
The in vitro susceptibilities of 128 isolates of east1+Escherichia coli from pre‐weaned and post‐weaned pigs with diarrhoea were tested with nine commonly used anti‐microbial agents by an agar dilution minimal inhibitory concentration (MIC) procedure according to National Committee for Clinical Laboratory Standards guidelines. For the isolates from pre‐weaned and post‐weaned pigs, most of them were susceptible to low concentrations (MIC90) of tetracycline (4 and 2 μg/ml), ceftiofur (2 and 2 μg/ml), and colistin (4 and 2 μg/ml). Marked resistance was found in others. 相似文献
107.
Chitin and chitosan have been extensively investigated as a matrix of organic/inorganic composite. Barium, one of the radiopaque inorganic materials, can provide chitosan with radiopaque property by blending of chitosan and BaSO4. The filtered and deaerated chitosan/BaCl2 solutions were extruded into NaOH and Na2SO4 coagulation bath through a nozzle by gear pump. BaSO4 was synthesized by the reaction between BaCl2 and Na2SO4 in the coagulation bath, in which acidic chitosan solution was also solidified at the same time. In XRD, the introduction of BaSO4 into chitosan fibers reduced the inherent peak of chitosan fibers. In angiographic observation, chitosan/BaSO4 hybrid fibers exhibited the clear contrast images which become clear with an increase in BaSO4 content. 相似文献
108.
Park BS Kim JR Lee SE Kim KS Takeoka GR Ahn YJ Kim JH 《Journal of agricultural and food chemistry》2005,53(4):1152-1157
The growth-inhibiting activity of anthraquinone-2-carboxylic acid and lapachol identified in the inner bark of taheebo, Tabebuia impetiginosa, toward 10 human intestinal bacteria was evaluated by using a paper disk diffusion bioassay and compared to those of seven lapachol congeners (1,4-naphthoquinone, naphthazarin, menadione, lawsone, plumbagin, juglone, and dichlone) as well as two commercially available antibiotics, chloramphenicol and tetracycline. Anthraquinone-2-carboxylic acid exhibited very strong growth inhibition of Clostridium paraputrificum at 1 microg/disk while 100 microg/disk of lapachol was needed for moderate growth inhibition of the same organism. These two isolates exhibited weak inhibition of Clostridium perfringens and Escherichia coli at 100 microg/disk while no adverse effects were observed on the growth of Bifidobacterium adolescentis, Bifidobacterium bifidum, Bifidobacterium infantis, Lactobacillus acidophilus, and Lactobacillus casei at 1000 microg/disk. Structure-activity relationships indicate that a methyl group in the C-2 position of 1,4-naphthoquinone derivatives might play an important role in antibacterial activity. 相似文献
109.
Perception of Teaching and Assessing Technical Proficiency in American College of Veterinary Surgeons Small Animal Surgery Residency Programs 下载免费PDF全文
110.