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Cede SUASTIKA Tomohide NATSUAKI Hirotsugu TERUI Takeshi KANO Hiroyuki IEKI Seiichi OKUDA 《Journal of General Plant Pathology》2001,67(1):73-77
The complete nucleotide sequence of a seedling-yellows-inducing isolate NUagA of Citrus tristeza virus (CTV) was determined. It consisted of 19302 nucleotides and contained 12 open reading frames (ORF) organized identically to
those of previously sequenced isolates. This genome is the largest among the CTV genome sequenced so far ; it is 6 nucleotides
(nt), 76 nt, 43 nt, and 53 nt longer than that of T36 (quick decline, Florida), VT (seedling yellows, Israel), T385 (mild,
Spain), and SY568 (stem pitting, California), respectively. Sequence comparison of NUagA and the other isolates revealed approximately
90% identities throughout the 3′ half of the genome. The 5′ half of the genome was only about 70% identical to that of T36
but still high at about 90% to those of VT, SY568, and T385. Comparison of amino acid sequences on ORF1a encoding polyproteins,
the most variable region, reflects the CTV isolate relationship ; NUagA is closely related to VT, SY568, and T385, but distantly
related to T36.
Received 29 May 2000/ Accepted in revised form 16 November 2000 相似文献
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Yoichi Kawazu Takahide Sasaya Toshiyuki Morikawa Keita Sugiyama Tomohide Natsuaki 《Journal of General Plant Pathology》2003,69(1):55-60
The coat protein (CP) gene of Mirafiori lettuce virus (MiLV), a tentative member of the genus Ophiovirus was isolated and sequenced. The established sequence consists of 1514 nucleotides including one open reading frame (ORF)
with 1311 nucleotides that encodes 437 amino acids with a relative molecular mass 48 543. When the ORF was expressed in Escherichia coli, the obtained protein was confirmed as CP by Western blotting using an antiserum against MiLV. Database searches showed that
the CP gene of MiLV has a sequence similar to that of Citrus psorosis virus (CPsV), the type species of the genus Ophiovirus. The comparison between MiLV and CPsV CP genes revealed that the identities of the nucleotide and amino acid sequences were
46.5% and 30.9%, respectively.
Received: July 29, 2002 / Accepted: October 2, 2002 相似文献
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ABSTRACT: In this study, the site of spawning ground and the relationship between oceanographic conditions and gonadal development and emaciation of the Japanese common squid Todarodes pacificus were examined based on data from research cruises. The research cruises were conducted in the south-western part of the Sea of Japan and the East China Sea in the winter of 1999. Sea surface temperature (SST) ranged 12.7–23.2°C in the survey area. Mature females concentrated on the continental shelf of the East China Sea, suggesting a major spawning ground was formed in this area. Gonadal development of the collected squid was examined using ovary somatic index (OSI) and oviduct somatic index (ODSI) in females, and emaciation was examined using mantle muscle somatic index (MI) and hepatosomatic index (HSI). Mean OSI and ODSI increased with SST at the stations where SST was greater than 15°C, particularly where SST ranged between 15 and 18°C. In contrast, mean MI and HSI decreased with increase in SST at the stations where SST was above 15°C. These results indicate that female gonads develop rapidly at SST above 15°C, and at the same time adult squids tend to be emaciated at this temperature condition. 相似文献
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In the southern part of the East China Sea (ECS), a large spawning ground of jack mackerel Trachurus japonicus has recently been found: Larval survival during the period of transport from the spawning ground along the shelf break is
potentially a critical stage prior to recruitment. As such, the distribution of copepod nauplii in this region was investigated
during the main spawning period in 2003–2006. The average naupliar density in 2003 was significantly higher than the other
years along the shelf break, which is a major transport pathway for jack mackerel larvae (sea-surface temperature 20–23°C).
Estimated egg production rates for Paracalanus spp., one of the most dominant genera of copepods in the southern ECS, based on temperature and chlorophyll-a concentration could not fully explain the spatial and annual variation in naupliar distribution and abundance. Although naupliar
densities showed significant positive correlations with chlorophyll-a concentration for all years, an analysis of covariance revealed that naupliar density in 2003 was high even if the effect
of chlorophyll-a concentration was excluded. This suggests that apart from copepod production, adult female distribution plays an important
role in variability of the naupliar distribution and abundance in the southern ECS. 相似文献
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During the course of development of muscle cells in certain tunicates, a sign of regenerative membrane response appears in the gastrula stage. In the early tadpole larva, the action potential consists of a spike followed by a plateau. The latter-disappears in fully differentiated cells, conceivably in association with the establishment of delayed rectification. 相似文献
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Dietary administration of probiotics to sows and/or their neonates improves the reproductive performance,incidence of post‐weaning diarrhea and histopathological parameters in the intestine of weaned piglets
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Teruo Hayakawa Tomohide Masuda Daisuke Kurosawa Takamitsu Tsukahara 《Animal Science Journal》2016,87(12):1501-1510
Probiotics have gained considerable attention with respect to their beneficial effects on livestock performance and health. The most significant effects of probiotics on the gut microbiota and the host animals take place when they are included in diets during particularly stressful periods such as weaning and/or at the beginning of the lactation period. The probiotics Bacillus mesentericus strain TO‐A at 1 × 108 colony forming units (CFU)/g, Clostridium butyricum strain TO‐A at 1 × 108 CFU/g and Enterococcus faecalis strain T‐110 at 1 × 109 CFU/g were used. Litter weight at delivery and ratio of return to estrous improved significantly (17% and 24% improvement, respectively) by probiotic administration to sows (0.2% (w/w)). Furthermore, the feed intake of the probiotics‐administered sows was greater than that of the control sows during the late lactation period. Post‐weaning diarrheal incidence and growth performance was improved by probiotics administration to neonates (0.02% (w/w)), while the combined use of probiotics in sows and their neonates induced the enlargement of villous height and prevented muscle layer thinning in the small intestine of weaning piglets. The administration of probiotics of three species of live bacteria improved the porcine reproductive performance around stressful periods of sows (farrowing) and piglets (weaning). [Corrections added on 26 April 2016, after first online publication: ‘Enterococcus faecalis strain T‐100’ has been corrected to ‘Enterococcus faecalis strain T‐110’ in the above paragraph and in the ‘Probiotics’ section under the Materials and Methods heading.] 相似文献
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Masashi Fujinaga Hideki Ogiso Hidetada Wakabayashi Toshiyuki Morikawa Tomohide Natsuaki 《Journal of General Plant Pathology》2009,75(2):157-159
A viral disease was found in Nagano Prefecture, Japan, on statice (Limonium sinuatum) with chlorotic leaf spot, necrotic stunt, and dwarfing. Spherical virus particles 30 nm in diameter were isolated from infected
plants and statice seedlings and caused identical symptoms 4 weeks after mechanical inoculation. Nucleotide and deduced amino
acid sequences of the coat protein showed 98% and 98.7% identities with those of Grapevine Algerian latent virus (GALV) nipplefruit strain. This is the first report in Japan of a viral disease on statice caused by GALV.
The nucleotide sequence data reported here are available in the DDBJ/EMBL/GenBank databases under accession AB461854. 相似文献