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871.
Seshadri R Adrian L Fouts DE Eisen JA Phillippy AM Methe BA Ward NL Nelson WC Deboy RT Khouri HM Kolonay JF Dodson RJ Daugherty SC Brinkac LM Sullivan SA Madupu R Nelson KE Kang KH Impraim M Tran K Robinson JM Forberger HA Fraser CM Zinder SH Heidelberg JF 《Science (New York, N.Y.)》2005,307(5706):105-108
872.
The semiconducting properties of a wide range of minerals are often ignored in the study of their interfacial geochemical behavior. We show that surface-specific charge density accumulation reactions combined with bulk charge carrier diffusivity create conditions under which interfacial electron transfer reactions at one surface couple with those at another via current flow through the crystal bulk. Specifically, we observed that a chemically induced surface potential gradient across hematite (alpha-Fe2O3) crystals is sufficiently high and the bulk electrical resistivity sufficiently low that dissolution of edge surfaces is linked to simultaneous growth of the crystallographically distinct (001) basal plane. The apparent importance of bulk crystal conduction is likely to be generalizable to a host of naturally abundant semiconducting minerals playing varied key roles in soils, sediments, and the atmosphere. 相似文献
873.
Weller GR Kysela B Roy R Tonkin LM Scanlan E Della M Devine SK Day JP Wilkinson A d'Adda di Fagagna F Devine KM Bowater RP Jeggo PA Jackson SP Doherty AJ 《Science (New York, N.Y.)》2002,297(5587):1686-1689
In eukaryotic cells, double-strand breaks (DSBs) in DNA are generally repaired by the pathway of homologous recombination or by DNA nonhomologous end joining (NHEJ). Both pathways have been highly conserved throughout eukaryotic evolution, but no equivalent NHEJ system has been identified in prokaryotes. The NHEJ pathway requires a DNA end-binding component called Ku. We have identified bacterial Ku homologs and show that these proteins retain the biochemical characteristics of the eukaryotic Ku heterodimer. Furthermore, we show that bacterial Ku specifically recruits DNA ligase to DNA ends and stimulates DNA ligation. Loss of these proteins leads to hypersensitivity to ionizing radiation in Bacillus subtilis. These data provide evidence that many bacteria possess a DNA DSB repair apparatus that shares many features with the NHEJ system of eukarya and suggest that this DNA repair pathway arose before the prokaryotic and eukaryotic lineages diverged. 相似文献
874.
Barrios-Rodiles M Brown KR Ozdamar B Bose R Liu Z Donovan RS Shinjo F Liu Y Dembowy J Taylor IW Luga V Przulj N Robinson M Suzuki H Hayashizaki Y Jurisica I Wrana JL 《Science (New York, N.Y.)》2005,307(5715):1621-1625
Signaling pathways transmit information through protein interaction networks that are dynamically regulated by complex extracellular cues. We developed LUMIER (for luminescence-based mammalian interactome mapping), an automated high-throughput technology, to map protein-protein interaction networks systematically in mammalian cells and applied it to the transforming growth factor-beta (TGFbeta) pathway. Analysis using self-organizing maps and k-means clustering identified links of the TGFbeta pathway to the p21-activated kinase (PAK) network, to the polarity complex, and to Occludin, a structural component of tight junctions. We show that Occludin regulates TGFbeta type I receptor localization for efficient TGFbeta-dependent dissolution of tight junctions during epithelial-to-mesenchymal transitions. 相似文献
875.
Food system resilience to climate change is uniquely imperative for bringing Sustainable Development Goals within reach and leaving no one behind. Food systems in East and Southeast Asia are interacting with planetary boundaries and are adversely affected by extreme weather-related events. A practical question for East and Southeast Asian stakeholders is how to foster climateresilient food systems in the face of lingering food system vulnerabilities and policy gaps. This paper reviews food syste... 相似文献
876.
Feinberg AW Feigel A Shevkoplyas SS Sheehy S Whitesides GM Parker KK 《Science (New York, N.Y.)》2007,317(5843):1366-1370
We demonstrate the assembly of biohybrid materials from engineered tissues and synthetic polymer thin films. The constructs were built by culturing neonatal rat ventricular cardiomyocytes on polydimethylsiloxane thin films micropatterned with extracellular matrix proteins to promote spatially ordered, two-dimensional myogenesis. The constructs, termed muscular thin films, adopted functional, three-dimensional conformations when released from a thermally sensitive polymer substrate and were designed to perform biomimetic tasks by varying tissue architecture, thin-film shape, and electrical-pacing protocol. These centimeter-scale constructs perform functions as diverse as gripping, pumping, walking, and swimming with fine spatial and temporal control and generating specific forces as high as 4 millinewtons per square millimeter. 相似文献
877.
878.
Jerry A. Ivany Jennifer Lea Kevin V. McCully H. Tapani Kunelius 《Crop Protection》2002,21(10):991-995
Lolium multiflorum Lam. (annual ryegrass) is used extensively as a forage crop and seed is produced in several areas worldwide. Seed production has been shown to be commercially possible in Atlantic Canada but improved weed control is needed. Studies were conducted in two provinces (Prince Edward Island and New Brunswick) to determine the effects of the herbicides bentazon, bromoxynil/MCPA, dicamba/MCPA, thifensulfuron/tribenuron, 2,4-D and MCPA on weed control, L. multiflorum growth, seed yields and seed germination in 1998 and 1999. The herbicides MCPA, dicamba/MCPA and bromoxynil/MCPA as evaluated in these experiments provided acceptable control of the broad-leaved weeds present and had no effect on seed yield or seed germination. Although the thifensulfuron/tribenuron caused early foliar injury, the plants recovered and it provided excellent weed control. None of the herbicides affected seed germination. MCPA, dicamba/MCPA and bromoxynil/MCPA could be used to provide control of broad-leaved weeds and thifensulfuron/tribenuron could be used if growers were willing to accept some early injury and possible yield loss to obtain the improved weed control. 相似文献
879.
Fatty Acid Composition of Three Rice Varieties Following Storage 总被引:5,自引:0,他引:5
Zhongkai Zhou Chris Blanchard Stuart Helliwell Kevin Robards 《Journal of Cereal Science》2003,37(3):327-335
The petroleum ether extractable lipids (PEE-L) and aqueous propan-1-ol extractable lipids (PWE-L) of three varieties of rice were determined gravimetrically and characterised by fatty acid profiles. The content of PEE-L (22·5–28·2 mg g−1) was higher than that of PWE-L (7·4–11·5 mg g−1) in brown rice with the situation reversed in milled rice (3·0–4·5 mg g−1vs. 7·2–8·7 mg g−1). The ratio of unsaturated fatty acid to saturated fatty acid was about two times higher in PEE-L than that in PWE-L for both brown and milled rice reflecting the selective complexation of saturated fatty acids. Rice storage at 37 °C resulted in some minor but statistically significant changes in the fatty acid profile. In the case of brown rice, the only notable changes were a reduction in the amounts of oleic and linoleic acids in the aqueous propan-1-ol extractable fatty acid fraction (PWE-FA) following storage at the higher temperature. Milled rice of all three varieties showed a decrease in linoleic acid content of PEE-L following storage at 37 °C for 4 and 7 months compared to storage at 4 °C. There was no change in fatty acid contents of PWE-L of milled rice when stored at 4 and 37 °C for 4 and 7 months. This implies that the PWE-L (or bound lipids) were more stable than PEE-L (or free lipids) during storage. 相似文献
880.
Thomas H. Hansen Enzo Lombi Melissa Fitzgerald Kristian H. Laursen Jens Frydenvang Søren Husted Chanthakhone Boualaphanh Adoracion Resurreccion Daryl L. Howard Martin D. de Jonge David Paterson Jan K. Schjoerring 《Journal of Cereal Science》2012
The effect of different polishing techniques on loss of mineral elements from rice grains was quantified using a panel of indica and tropical japonica genotypes, previously classified as differing in ease of polishing. Gradients in mineral elements across the bran-endosperm interface were quantified using micro-scaled precision abrasive polishing in combination with inductively coupled plasma mass spectrometry and synchrotron X-ray fluorescence microscopy. Frictional polishing, similar to that of commercial mills, i.e. 8–10% loss of grain weight, reduced the concentration of Fe, Mg, P, K and Mn by 60–80% in all genotypes. Following gentler polishing (3–5% weight loss), genotypes classified as difficult to polish showed smaller decreases in Fe, Mg, P, K and Mn compared to genotypes classified as easy to polish. The concentration of other elements, e.g. Zn, S, Ca, Cu, Mo and Cd, showed comparable reductions (<30%) irrespective of polishing technique or ease of polishing. The different patterns of polishing losses of minerals reflected their distribution within the grain. Five-fold differences in the reduction of Zn concentration during polishing were observed for different genotypes which started with similar Zn concentrations in the unpolished grain, thus showing clear potential for selecting genotypes with reduced polishing losses of Zn. 相似文献