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31.
Fry FH Okarter N Baynton-Smith C Kershaw MJ Talbot NJ Jacob C 《Journal of agricultural and food chemistry》2005,53(3):574-580
Allicin, an active ingredient of garlic, possesses a range of antimicrobial properties. Unfortunately, certain properties of the compound, such as chemical instability and low miscibility with water, have hampered its practical use in the past. Here, we show that it is possible to use a binary system consisting of the plant enzyme alliinase and its substrate alliin to generate allicin, and hence antifungal activity, in situ. During application, the two inactive components generate compounds that inhibit growth and infection-related development of the rice blast fungus Magnaporthe grisea. It is therefore possible to "trigger" biological activity in a controlled, yet effective manner. Apart from circumventing many of the drawbacks of allicin, this binary system has additional important advantages, such as low toxicity of its individual components and selective activation. Importantly, alliinase is also able to use different substrates, therefore paving the way to a range of novel, binary antimicrobial systems with custom-made chemical and biochemical properties. 相似文献
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Christina A. Chambers Sarah E. Smith F.A. Smith M.D. Ramsey D.J.D. Nicholas 《Soil biology & biochemistry》1980,12(2):93-100
The symbioses between Trifolium subterraneum, mycorrhizal fungi and Rhizohium are affected by (NH4)2SO4 and by the nitrification inhibitors 2-chloro-6 (trichloromethyl) pyridine (N-Serve) and 2-trichloromethyl pyridine (2TMP). At 50 μg · g?1 soil N-Serve and 2TMP had toxic effects on plant growth, measured as leaf expansion, root length and dry weight. Lower concentrations of N-Serve also produced some toxic symptoms. The addition of (NH4)2SO4 to the soil at 2 and 6 m-equiv NH+4 per pot, resulted in reduced root length and nodulation. Shoot dry weight was reduced at 6 m-equiv NH+4 per pot. In the presence of (NH4)2SO4 the toxic effects of the nitrification inhibitors on plant growth were less.Both nitrification inhibitors reduced development of mycorrhizal entry-points and extent of root colonization (% infection). Percentage infection of the root system was also reduced by (NH4)2SO4. Development of nodules on the lateral roots was increased in the presence of N-Serve at 5 and 15 μ · g?1. This effect, however, was accompanied by a marked reduction in N2ase activity. Smaller increases in nodulation were apparent with 2TMP and were associated with variable N2ase activity. 相似文献
34.
Groups of three-week-old chickens were given graded doses of inactivated canine parvovirus vaccines. Blood samples were taken three weeks later and the sera examined by ELISA for antibodies to canine parvovirus. Reproducible, linear, log-dose serological responses were observed, enabling the potency of the vaccines to be compared. The simultaneous administration of other components of canine multivalent vaccines appeared to reduce the response to the parvovirus component. When its results have been correlated with the degree of protection in dogs this test could be used to assess the potency of inactivated canine parvovirus vaccines. 相似文献
35.
Leland C. Dickey Michael J. Kurantz Nicholas Parris 《Industrial Crops and Products》2008,27(3):303-307
The many recent dry-grind plants that convert corn to ethanol are a potential source of substantial amounts of corn oil, if an economical method of separating it can be developed. Oil separation from corn germ by aqueous extraction (AE) was studied. Batches of 5–20% germ in a buffer solution were preheated in a pressure cooker, ground in a blender or colloid mill, churned in an incubator/shaker and centrifuged to separate a free oil fraction. The highest oil yields were obtained when the germ mass fraction was between 0.1 and 0.16. Addition of an enzyme solution to the ground germ suspension prior to churning, aqueous enzymatic extraction (AEE) gave an oil yield twice as high as the AE yield, carried out using similar conditions. Both types of extraction produced the most oil after a 122 °C cook followed by churning at 160 rpm and 70 °C. Oil yield increased linearly with dispersion loading, from 0.5 up to 1.0 g/cm2 based on beaker cross-section (0.09–0.12 germ mass fraction). Over a several month period the germ used for the extractions became more difficult to extract, despite storage at 4 °C. 相似文献
36.
Tom MISSELBROOK Zhaohai BAI Zejiang CAI Weidong CAO Alison CARSWELL Nicholas COWAN Zhenling CUI David CHADWICK Bridget EMMETT Keith GOULDING Rui JIANG Davey JONES Xiaotang JU Hongbin LIU Yuelai LU Lin MA David POWLSON Robert M. REES Ute SKIBA Pete SMITH Roger SYLVESTER-BRADLEY John WILLIAMS Lianhai WU Minggang XU Wen XU Fusuo ZHANG Junling ZHANG Jianbin ZHOU Xuejun LIU 《农业科学与工程前沿(英文版)》2022,9(3):475
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38.
Andrew S. Brierley Paul G. Fernandes Mark A. Brandon Frederick Armstrong Nicholas W. Millard Steven D. McPhail Peter Stevenson Miles Pebody James Perrett Mark Squires Douglas G. Bone Gwyn Griffiths 《Fisheries Research》2003,60(2-3):569-576
The autonomous underwater vehicle (AUV) Autosub-2 was deployed on eight missions ahead of RRS James Clark Ross in the northern Weddell Sea and in the Bransfield Strait, Southern Ocean, to assess avoidance of the research vessel by Antarctic krill Euphausia superba. The AUV was equipped with the same type of scientific echosounder as the research vessel (Simrad EK500 operating at 38 and 120 kHz) and measured the density of krill along transect acoustically (g m−2 wet mass) prior to the ship’s arrival. We hypothesised that if krill avoided the ship, perhaps in response to radiated noise, then the ship should detect less krill than the AUV which is known to have much lower noise levels than the ship. We were unable to detect any significant difference between the density of krill detected by the ship or the AUV, either at the transect level or at finer scales within transects. We conclude, therefore, that avoidance by krill of RRS James Clark Ross will not significantly bias acoustic estimates of krill abundance by this vessel. 相似文献
39.
The expansion of oil palm monocultures into globally important Southeast Asian tropical peatlands has caused severe environmental damage. Despite much of the current focus of environmental impacts being directed at industrial scale plantations, over half of oil palm land-use cover in Southeast Asia is from smallholder plantations. We differentiated a first generation smallholder oil palm monoculture into 8 different sampling zones, and further divided the 8 sampling zones into oil palm root influenced (Proximal) and reduced root influence (Distal) areas, to assess how peat properties regulate in situ carbon dioxide (CO2) and methane (CH4) fluxes. We found that all the physico-chemical properties and nutrient concentrations except sulphur varied significantly among sampling zones. All physico-chemical properties except electrical conductivity, and all nutrient content except nitrogen and potassium varied significantly between Proximal and Distal areas. Mean CO2 fluxes (ranged between 382 and 1191 mg m−2 h−1) varied significantly among sampling zones, and between Proximal and Distal areas, with notably high emissions in Dead Wood and Path zones, and consistently higher emissions in Proximal areas compared to Distal areas within almost all the zones. CH4 fluxes (ranged between −32 and 243 µg m−2 h−1) did not significantly vary between Proximal and Distal areas, however significantly varied amongst sampling zones. CH4 flux was notably high in Canal Edge and Understorey Ferns zones, and negative in Dead Wood zone. The results demonstrate the high heterogeneity of peat properties within oil palm monoculture, strengthening the need for intensive sampling to characterize a land use in the tropical peatlands. 相似文献
40.
Kristen C. Nelson Rachel F. Brummel Nicholas Jordan Steven Manson 《Agriculture and Human Values》2014,31(2):245-259
Multifunctional agricultural systems seek to expand upon production-based benefits to enhance family wellbeing and animal health, reduce inputs, and improve environmental services such as biodiversity and water quality. However, in many countries a landscape-level conversion is uneven at best and stalled at worst. This is particularly true across the eastern rural landscape in the United States. We explore the role of social networks as drivers of system transformation within dairy production in the eastern United States, specifically rotational grazing as an alternative management option. We hypothesize the importance of weak ties within farmer social networks as drivers of change. In Wisconsin, Pennsylvania, and New York, we conducted 53 interviews with confinement, low-intensity, and rotational grazing dairy producers as well as 35 interviews with associated network actors. Though confinement and grazier networks had similar proportions of strong and weak ties, confinement producers had more market-based weak ties, while graziers had more weak-ties to government agencies and other graziers in the region. These agency weak ties supported rotational graziers through information exchange and cost sharing, both crucial to farmers’ transitions from confinement-based production to grazing systems. While weak ties were integral to initial innovation, farmers did not maintain these relationships beyond their transition to grazing. Of equal importance, grazier weak-tie networks did not include environmental organizations, suggesting unrealized potential for more diverse networks based on environmental services. By understanding the drivers, we can identify barriers to expanding weak tie networks and emergent properties in order to create institutions and policies necessary for change. 相似文献