Veterinary Randomized Clinical Trial Reporting: A Review of the Small Animal Literature

The randomized clinical trial (RCT) is a valuable research method for the evaluation of new treatment and prevention regimens in veterinary medicine. Reporting of clinical trials in other disciplines has not been complete. Without complete information on the conduct and results of a clinical trial, readers cannot optimize their use of the information presented. This report represents an objective review of randomized clinical trials in the veterinary small animal literature from 1986 to 1990. Results indicate that RCT reports in the small animal veterinary literature are incomplete. The importance of reporting on particular aspects of RCT research is described.     

Detection of a pigeon pea witches'-broom-related phytoplasma in trees of Gliricidia sepium affected by little-leaf disease in Central America

The polymerase chain reaction (PCR) incorporating phytoplasma-specific ribosomal RNA primer pair P1 and P7 consistently amplified a product of expected size (1.8 kb) when template DNA for PCR was extracted from leaf and shoot samples of Gliricidia sepium from Honduras exhibiting symptoms of little-leaf disease (LLD). Restriction fragment length polymorphism analysis (RFLP) of amplified rDNA products indicated that phytoplasmas detected in LLD-affected G. sepium were very similar although not identical to phytoplasmas associated with pigeon pea witches'-broom (PPWB) disease in the western Caribbean region. Phytoplasma detection in trees was enhanced by reamplification of the P1/P7-primed PCR products with nested primers PPf1 and Tint. Nested reactions enabled additional positive detections in foliage of several G. sepium trees showing only mild or no apparent LLD symptoms. Neither rDNA RFLP nor sequence analyses of 16–23 S rRNA spacer regions revealed differences among Honduran LLD phytoplasma or between these strains and phytoplasmas detected in G. sepium with LLD-like symptoms in El Salvador, Guatemala, and Nicaragua, indicating that closely related, possibly identical, pathogens presently affect this tree species in all four countries.     

Protides of the Mustelidae: immunoresponse of mustelids to Aleutian mink disease virus.

Members of North American Mustelidae were tested for their response to inoculation with 10(6) infective doses of Aleutian disease virus. In subfamily Mustelinae, 3 species in the genus Mustela (M vision, M erminea, and M putorius) and 2 species in genus Martes (Ma pennanti and Ma americana) responded immunologically with some features resembling Aleutian disease in mink. In subfamily Mephitinae, only Mephitis mephitis responded, and others of the subfamily did not, nor did members of subfamilies Melinae and Lutrinae. The responses observed ranged from development of detectable antibody levels determined by counterimmunoelectrophoresis to histopathologic changes typical of Aleutian disease.