全文获取类型
收费全文 | 845篇 |
免费 | 44篇 |
国内免费 | 8篇 |
专业分类
林业 | 102篇 |
农学 | 34篇 |
基础科学 | 3篇 |
125篇 | |
综合类 | 43篇 |
农作物 | 19篇 |
水产渔业 | 114篇 |
畜牧兽医 | 376篇 |
园艺 | 16篇 |
植物保护 | 65篇 |
出版年
2024年 | 2篇 |
2023年 | 3篇 |
2022年 | 20篇 |
2021年 | 30篇 |
2020年 | 14篇 |
2019年 | 13篇 |
2018年 | 15篇 |
2017年 | 25篇 |
2016年 | 28篇 |
2015年 | 42篇 |
2014年 | 34篇 |
2013年 | 43篇 |
2012年 | 60篇 |
2011年 | 54篇 |
2010年 | 42篇 |
2009年 | 38篇 |
2008年 | 72篇 |
2007年 | 68篇 |
2006年 | 49篇 |
2005年 | 53篇 |
2004年 | 45篇 |
2003年 | 52篇 |
2002年 | 38篇 |
2001年 | 16篇 |
2000年 | 11篇 |
1999年 | 2篇 |
1998年 | 11篇 |
1997年 | 5篇 |
1996年 | 3篇 |
1995年 | 1篇 |
1994年 | 3篇 |
1993年 | 1篇 |
1987年 | 1篇 |
1984年 | 1篇 |
1980年 | 1篇 |
1977年 | 1篇 |
排序方式: 共有897条查询结果,搜索用时 0 毫秒
31.
Kenji Kawashima Ken Katsuda Hiroshi Tsunemitsu 《Journal of veterinary diagnostic investigation》2007,19(1):60-68
To investigate the prevalence and features of postweaning multisystemic wasting syndrome (PMWS) in Japan, an epidemiological study was conducted in 692 weaned pigs with various clinical signs, commonly including wasting or weight loss, collected from 129 swine farms between 2000 and 2003. The presence of PMWS was diagnosed by the detection of characteristic histological lesions and moderate to large amounts of porcine circovirus type 2 (PCV2) antigen within the lesions in multiple lymphoid tissues. Postweaning multisystemic wasting syndrome was positive in 23.4% of pigs (162/692) over the course of the study, and occurred in 50.4% of the farms (65/129). Mortality in 30-120-day-old pigs in the farms positive for PMWS varied from 0.1 to 32.0%. No significant difference in mortality was seen between PMWS-positive and -negative farms (P = 0.1). However, mortality was significantly higher in the PMWS-positive farms where PMWS was diagnosed in more than 50% of the pigs examined compared to farms negative for PMWS (P = 0.02). These findings indicate that PMWS has spread widely in Japan. Moreover it may exist in variable forms in swine farms, including an epidemic form or a subtle endemic or sporadic form. A case-control study suggested that risk factors for the occurrence of PMWS include porcine reproductive and respiratory syndrome (PRRS) pneumonias and Mycoplasma hyorhinis infection. 相似文献
32.
33.
本研究利用单向水平板淀粉凝胶电泳以我国5个固有地方绵羊品种包括湖羊(Hu)、同羊(Tong)、滩羊(Tan)、小尾寒羊(Han)、洼地羊(WD)为研究对象对X-蛋白遗传多样性进行了检测,并引用我国周边国家、地区绵羊品种为分析背景。结果表明以喜马拉雅山脉为界的亚洲东部和南部的北部群体和南部群体在编码的X(+)型的显性等位基因X频率上存在极显著差异(P〈0.01)。北部检测的群体中X等位基因频率范围为0~0.18,平均值为0.0878,包括属于藏羊和蒙古羊系统的小尾寒羊(Han)、不丹羊(Bhutan)、同羊(Tong)、湖羊(Hu)、滩羊(Tan)、洼地绵羊(WD)、Bhyanglung绵羊(Bhy)、Baruwal绵羊(Bar)、云南绵羊(Yunnan)、哈拉和林绵羊(Kh)和乌兰巴托绵羊(Ub)。南部检测的群体中X等位基因频率范围为0.2037~0.4655,平均值为0.3082,包括属于印度绵羊系统的孟加拉国绵羊(Ban)、Kagi绵羊(Kagi)、Lampuchhre绵羊(Lamp)、越南占部落绵羊(Cham)和缅甸绵羊(Mya)。本研究揭示X等位基因可作印度绵羊的标记,在绵羊群体尤其是亚洲东部和南部绵羊系统形成的研究中具有潜在的重要作用。 相似文献
34.
35.
Matsuo S Hayashi S Watanabe T Adachi K Miyoshi A Kato A Suzuki M 《Journal of toxicologic pathology》2009,22(4):289-292
Emphysematous cystitis is a rare disorder caused by bacterial infection and characterized by gas accumulation within the bladder wall with cyst formation. This report describes the histopathological characteristics of emphysematous cystitis found in a diabetic female beagle induced by streptozotocin and alloxan. Macroscopically, multiple cyst-like structures were observed on the cut surface of the urinary mucosa. During fixation, small specimens cut from the mucosa floated on the surface of the fixative solution. Histopathologically, multiple cysts were lined with a single layer of flattened cells found to be immunohistochemically positive for vimentin, partially positive for α-smooth muscle actin or macrophage scavenger receptor, class A, and thought to be myofibroblasts, fibroblasts or macrophages. Multinucleated giant cells were observed around the cysts, and gram-negative short bacilli were observed in the lumen of the urinary bladder. From these findings, this case was diagnosed as emphysematous cystitis. 相似文献
36.
37.
Isolation and culture of rabbit primordial germ cells 总被引:2,自引:0,他引:2
Kakegawa R Teramura T Takehara T Anzai M Mitani T Matsumoto K Saeki K Sagawa N Fukuda K Hosoi Y 《The Journal of reproduction and development》2008,54(5):352-357
Primordial germ cells (PGCs) are embryonic precursors of the gametes of adult animals and are considered stem cells of the germline. Since their proliferation in vitro correlates well with the schedule of developmental changes in vivo, they might be interesting research tools for genomic imprinting, germ-cell tumors and fertility. Furthermore, once primordial germ cells are separated and placed on a feeder layer with cytokines, they become cultured pluripotent cell lines called embryonic germ (EG) cells. EG cells share several important characteristics with embryonic stem (ES) cells as they can also contribute to the germ line of chimeras. To investigate the characteristics of PGCs and establish rabbit EG (rEG) cells, we cultured rabbit PGCs (rPGCs) in vitro with various combinations of leukemia inhibitory factor (LIF), basic fibroblast growth factor (bFGF) and forskolin on inactivated mouse embryonic fibroblast (MEF) feeder layers. The present study found PGC proliferation in early cultures and induction of rEG-like colonies. These cells expressed pluripotent markers, such as alkaline phosphatase activity, OCT-4, Sox-2 and SSEA-1, in the undifferentiated state; however, the cells did not develop into a teratoma when injected into the kidney capsules of SCID mice, although the restricted differentiation potentials to neural cells were determined via embryoid body formation. From these characteristics and further characterization of the germ stem cell markers Vasa, SCP-1 and SCP-3, we suggested that these were hybrid cells with characteristics somewhere between PGC and EG cells. 相似文献
38.
39.
Caffeine in fertilization medium is not essential for bovine IVF by fully capacitated spermatozoa 总被引:1,自引:0,他引:1
The purpose of this study was to examine: 1) whether caffeine in the fertilization medium under mineral oil is essential for bovine in vitro fertilization by fully capacitated spermatozoa, 2) the minimum concentration of caffeine that shows an adverse effect on the motility of preincubated spermatozoa. Cumulus-oocyte complexes with heterogeneous-appearing ooplasm were matured in in vitro culture for 24 h and used for insemination. The fertilization rates of the preincubated spermatozoa introduced into the fertilization medium containing 0 mM or 5 mM caffeine were examined. The fertilization rate of the spermatozoa introduced into the medium without caffeine (final concentration of caffeine at fertilization was 0.27-0.35 mM) was significantly higher than that in the medium with 5 mM caffeine (82.4% vs 55.2%, P<0.05). When the final concentration of caffeine at fertilization was reduced ten-fold (0.02-0.03 mM), the fertilization rate was not significantly improved (86.0%). The motility of the preincubated spermatozoa introduced into the fertilization medium containing 0-5 mM caffeine was examined. The sperm motility in the fertilization medium without caffeine was significantly higher than that in the fertilization medium with more than 2 mM caffeine. These results indicate that caffeine in the fertilization medium is not essential for bovine in vitro fertilization by fully capacitated spermatozoa, and that more than 2 mM caffeine has an adverse effect on preincubated (capacitated) sperm motility. 相似文献
40.
Shohei KOBAYASHI Natsuko KAWANO Kenji MIYADO Ryo OHTA Takahiro AKIMOTO Taichi HATAKEYAMA Maiko KAWAGUCHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(1):153
Tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) is widely used as a flame retardant and is known to exhibit anti-androgenic effects in vitro and in vivo. To assess the reproductive toxicity potency of TDCIPP, we investigated the effects of 7 days of TDCIPP oral administration on epididymal sperm motion and concentration in adult male Wistar–Imamichi rats. Thirty-five days after the final administration, sperm parameters were evaluated by computer-assisted sperm analysis. Results showed that sperm swimming progression and vigor and sperm concentration in TDCIPP-treated rats were unexpectedly higher than those in control rats. TDCIPP did not significantly affect the percentage of motile sperms or sperm swimming pattern. These results contribute to the understanding of the biological effects of TDCIPP. 相似文献