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111.
Suzuki Toshiyuki Kamiyama Takashi Okumura Yutaka Ishihara Kenji Matsushima Ryoji Kaneniwa Masaki 《Fisheries Science》2009,75(4):1039-1048
Fatty-acid esters of dinophysistoxin-1 (DTX1) in scallops Patinopecten yessoensis, mussels Mytilus coruscus, and toxic dinoflagellate Dinophysis species, collected from Japanese seawater, were analyzed by liquid-chromatography mass spectrometry (LC–MS). Precursor ion
monitoring, multiple reaction monitoring for 18 fatty-acid esters of DTX1, and full-scan MS/MS spectra obtained with a hybrid
triple–quadrupole linear-ion-trap mass spectrometer showed that 14:0, 16:0, and 16:1 esters were the most abundant 7-O-acyl-DTX1 analogues in bivalves. Fatty-acids esters formed by conjugations at hydroxyl positions other than the 7-position
of DTX1 were not detected in the bivalves. DTX1 and okadaic acid-16:0 fatty-acid esters have been reported as the most abundant
ester in bivalves in several previous studies; however, we found that 7-O-16:1-DTX1 was the most abundant ester in some mussels in which 16:1 was more dominant than 16:0 in the free fatty-acid profile.
Comparison between 7-O-acyl-DTX1 and free fatty-acid profiles in the same bivalve samples suggests that polyunsaturated fatty acids are selectively
excluded in enzymatic acylation of DTX1. No 7-O-acyl-DTX1 was detected in any single-cell isolates of D. fortii, D. acuminata, D. mitra, D. norvegica, D. tripos, D. infundibulus, and D. rotundata. 相似文献
112.
Inwoo Bae Kiyoshi Osatomi Asami Yoshida Atsuko Yamaguchi Katsuyasu Tachibana Tatsuya Oda Kenji Hara 《Fisheries Science》2009,75(3):765-770
A translucent collagen gel was formed from a transparent acidic solution of red stingray collagen by adjusting to physiological
ionic strength and pH in phosphate buffer and then incubating at 25–37°C. During fibril formation from red stingray collagen,
the turbidity increased when the NaCl concentration was increased at constant pH and the rate of fibril formation was accelerated
by higher pH or lower NaCl concentration. The T
m of red stingray collagen fibrillar gel was estimated as 44.3 ± 3.5°C, which was higher than that of the collagen solution,
33.2°C. In addition, red stingray collagen gel maintained its shape without melting and was suitable for culture of mouse
stromal cells at 37°C. 相似文献
113.
Ryo TAKEUCHI Satoshi TAKAGI Kenji HOSOYA Hiroshi OHTA Takaharu ITAMI Masahiro OKUMURA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(11):1740
Canine gastroesophageal intussusception (GEI) is a rare and life-threatening condition that requires prompt diagnosis and treatment. A 19-day-old Siberian Husky with a 4-day history of regurgitation was diagnosed with GEI based on the findings of computed tomography (CT) performed without anesthesia. Endoscopic reduction of intussusception was impossible; thus, surgical reduction by traction of the duodenum was performed. CT revealed improvement of megaesophagus 82 days postoperatively. Eleven months postoperatively, fluoroscopy showed recovery to nearly normal esophageal motility. Two years postoperatively, no clinical signs were reported. CT is useful to diagnose GEI in neonate puppies with poor abdominal fat and to assess the gastric edema and the anatomical association of stomach with other organs. Fluoroscopy is helpful for evaluating postoperative esophageal motility. 相似文献
114.
Masaki Kiryu Miho Hamanaka Kayo Yoshitomi Susumu Mochizuki Kazuya Akimitsu Kenji Gomi 《Journal of General Plant Pathology》2018,84(3):221-229
Plant volatile compounds, including terpenes, are known to be involved in the rice defense system. In the present analysis of a terpene synthase, OsTPS18, in rice, we found that OsTPS18 was localized in the cytoplasm and synthesized the sesquiterpenes (E)-nerolidol and (E)-β-farnesene. The amounts of (E)-nerolidol and (E)-β-farnesene increased after jasmonic acid (JA) treatment. (E)-Nerolidol had significant antibacterial activity against Xanthomonas oryzae pv. oryzae (Xoo). These results suggest that (E)-nerolidol plays an important role in JA-induced resistance against Xoo and that it functions as an antibacterial compound in rice. 相似文献
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118.
Hiromi KEINO Atsushi FUNAHASHI Hiroomi KEINO Chihiro MIWA Masanori HOSOKAWA Yoshihiro HAYASHI Kenji KAWAKITA 《Journal of Equine Science》2009,20(4):79-88
In this study, we applied a novel psycho-educational horseback riding (PEHR) program to
the treatment of four Japanese children with pervasive developmental disorders (PDD) in
order to facilitate the acquisition of verbal and nonverbal communication skills. The
behavioral changes in each child were evaluated using a psychological and behavioral
scale. The scale for evaluating the effect of Human-Equips-Interaction on Mental activity
(HEIM scale) was designed to assess the behavioral improvement of children based on the
following 10 items: Human relationships, Imitation, Emotional expression, Sudden physical
movement, Fixative behavior, Adaptation to change, Visual response, Fear or nervousness,
and Verbal and nonverbal communication. After taking part in the PEHR program for several
months, all subjects showed remarkably improved HEIM scores and marked improvements were
observed in eye contact with others (instructors, side walkers, and leaders) in the riding
area. A statistical difference was found in items 1, 2, 3, 6, 7, 8, and 9. However, no
statistical difference was found in items 4, 5, and 10. As the program progressed, the
children showed enhanced verbal and nonverbal communication skills, and became more
expressive in their emotional and empathetic interaction with their parents. These
observations suggest that the normal functioning of pleasurable emotions and empathy may
facilitate further improvements in joint attention, imitation and empathy, and may result
in successful verbal expression by PDD children. Therefore, horseback riding can play a
very important role in the psycho-educational support required for the communication
ability of PDD children. 相似文献
119.
Kenji UCHIDA Keiko AKASHI Hidemasa MOTOSHIMA Tadasu URASHIMA Ikichi ARAI Tadao SAITO 《Animal Science Journal》2009,80(2):187-192
We analyzed the microbiota of domestic ropy fermented milk, Caspian Sea yogurt (or 'kasupikai yohguruto' in Japanese), circulated in Japan. We collected six varieties from five localities. Lactococcus ( L) lactis ssp. cremoris was isolated from all samples as the dominant strain at levels of 108 −109 CFU/g. We show this strain produces an extracellular polysaccharide (EPS) that causes the unique characteristic viscosity of the product. From analysis of the RAPD pattern of 60 bacterial isolates from the six samples, we found that 59 strains from a total of 60 isolates were identical and produced this viscosity. Furthermore, PFGE analysis of representative strains from each sample indicated that the isolates could be classified into four subgroups. This suggests these L. lactis ssp. cremoris strains found in Caspian Sea yogurt may have been slightly mutated during subculture in Japan. In addition, Lactobacillus (L.) sakei ssp. sakei were isolated from three samples; L. plantarum , Gluconoacetobacter sacchari and Acetobacter aceti were isolated from two samples; and L. paracasei , L. kefiri , Leuconostoc ( Leu. ) mesenteroides were isolated from one sample. 相似文献
120.
Matsuo S Hayashi S Watanabe T Adachi K Miyoshi A Kato A Suzuki M 《Journal of toxicologic pathology》2009,22(4):289-292
Emphysematous cystitis is a rare disorder caused by bacterial infection and characterized by gas accumulation within the bladder wall with cyst formation. This report describes the histopathological characteristics of emphysematous cystitis found in a diabetic female beagle induced by streptozotocin and alloxan. Macroscopically, multiple cyst-like structures were observed on the cut surface of the urinary mucosa. During fixation, small specimens cut from the mucosa floated on the surface of the fixative solution. Histopathologically, multiple cysts were lined with a single layer of flattened cells found to be immunohistochemically positive for vimentin, partially positive for α-smooth muscle actin or macrophage scavenger receptor, class A, and thought to be myofibroblasts, fibroblasts or macrophages. Multinucleated giant cells were observed around the cysts, and gram-negative short bacilli were observed in the lumen of the urinary bladder. From these findings, this case was diagnosed as emphysematous cystitis. 相似文献