Partial replacement of fish meal with plant protein sources using organic acids to practical diets for juvenile yellowtail,Seriola quinqueradiata

An experiment was conducted to investigate the effects of phosphorus (P), citric acid (CA) and formic acid (FA) supplementation on growth and loading of nitrogen (N) and phosphorus (P) in juvenile yellowtail fed fish meal (FM) and alternate plant protein (APP) diets. Six diets designated as F49 (490 g kg^?1 FM‐based), F49 + P (490 g kg^?1 FM with 5 g kg^?1 inorganic P), F31 + CA (310 g kg^?1 FM containing 200 g kg^?1 APP with 5 g kg^?1 CA), F31 + FA (310 g kg^?1 FM having 200 g kg^?1 APP with 4 g kg^?1 FA), F23 + CA (230 g kg^?1 FM containing 300 g kg^?1 APP with 5 g kg^?1 CA) and F23 + FA (230 g kg^?1 FM having 300 g kg^?1 APP with 4 g kg^?1 FA) were formulated. Yellowtails were fed one of the diets for 12 weeks under on‐site conditions at water temperature 22.0–27.0 °C. F49 + P gave the best growth, while F23 + CA the lowest. Specific growth rate and feed conversion ratio of F31 + CA were similar with control group. Addition of CA to APP diets significantly increased retention of P; hence, its excretion was lowered. The results of this study suggest that FM could be replaced up to 70% with the addition of 5 g kg^?1 CA to a low P‐containing plant protein sources diet without inorganic P supplementation in juvenile yellowtail diets, which in turn would enable a reduction in environmental pollution from aquafeeds.     

Comparing the carbon sequestration capacity of temperate deciduous forests between urban and rural landscapes in central Japan

Urbanisation is increasing tremendously in some parts of the world. Consequently, many rural forests may become depleted, although many opportunities exist for urban forests to increase. However, few studies have quantified the carbon (C) sequestration capacities of urban and rural forests in specific climatic zones. The present study compared carbon sequestration in two temperate deciduous forests located in Nagoya and Toyota, central Japan. The Nagoya University forest represented an urban forest, and a site in Toyota represented a rural forest. The urban forest at Nagoya University had comparatively smaller areas of green space and larger areas of buildings and roads. Land uses for building and road, which are typical of urban areas, result in smaller diurnal temperature ranges but higher air temperature, vapour pressure deficit, and atmospheric carbon dioxide (CO₂) concentration. The urban forest in this study exhibited higher gross ecosystem exchange (GEE), especially in the active growing season from May to September, suggesting the possible effect of CO₂ fertilisation. However, higher air temperatures caused comparatively smaller net ecosystem exchange (NEE) because of higher ecosystem respiration (RE). Although both forests functioned as CO₂ sinks at annual time scales, the rural Toyota forest site (5.43 t C ha^–1 yr^–1) had 36% higher net ecosystem production (NEP=–NEE; the negative sign indicates uptake by the forest ecosystem from the atmosphere) than that at the urban forest. The higher normalised respiration (i.e., RE/GPP ratio; GPP=–GEE where GPP represents gross primary production) at the Nagoya University forest might be attributable to factors associated with the degree of urbanisation. Thus, in temperate forests, factors associated with urbanisation may reduce the atmospheric carbon sink function by accelerating respiration. This is an issue of global interest, as many countries are experiencing rapid urbanisation.     

Pathogenic characters of Japanese potato strains of Ralstonia solanacearum

Ralstonia solanacearum (Rs) strains in phylotypes I and IV isolated from potato in Japan were investigated for pathogenicity on potato, tomato, eggplant, Solanum integrifolium, tobacco, groundnut, and pumpkin. The strains were divided into 17 types based on differences in their pathogenicity on the tested plants. Particularly, the pathogenicity of most phylotype I strains on eggplant was distinctly different from that of the phylotype IV strains. When nine potato varieties (included two breeding lines) were inoculated with several Rs strains, phylotype IV strains were highly virulent on the breeding lines that are regarded as resistant to phylotype I strains.     

Effect of Introduction of Irrigation on Technical Efficiency of Olive-Growing Farms in Tunisia

This paper investigates the effect of the introduction of an irrigation technique on the technical efficiency of olive production in a sample of olive-growing farms in Tunisia, estimating the Cobb-Douglas form of stochastic frontier production function. Results indicate that estimated technical efficiency scores vary, ranging from a minimum of 3.0% to a maximum of 91.2% with a mean value of 61.2%. This suggests that olive-growing farms in Tunisia can increase their production on average by 38.8% through more efficient use of technology and inputs. The introduction of irrigation increased productivity, however, the estimated technical efficiency of irrigated olive farms varies across farms and they are less efficient than non-irrigated farms. This finding suggests that the introduction of irrigation to non-irrigated farms has the potential to increase production levels, however, the current production of the irrigated farms is far from the ＂best practice frontier＂ that realizes maximum possible output. Accumulation of experience and knowledge by farm owners and selection of olive cultivar significantly contribute to improving technical efficiency.     

Footpad dermatitis in broiler chickens in Japan

The prevalence of footpad dermatitis (FPD) in broiler chickens in Japan was investigated. In the first examination at slaughterhouses, lesions were commonly observed on the footpads of a total of 8,985 broiler chickens from 45 flocks on 36 farms. In 3 flocks, all the birds examined had lesions. In the other 42 flocks, the incidence of FPD ranged from 31.9% (81/254) to 99.5% (1/222). The footpad lesions were classified into 4 categories according to the severity of dermatitis as follows; score 0, 1,181 birds (13.1%); score 1, 2,992 birds (33.3%); score 2, 3,000 birds (33.4%); and score 3, 1,812 birds (20.2%). The mean scores of the flocks varied widely from 0.31 to 2.69. Males had higher mean scores than females. No significant differences were found between the mean scores of birds reared in windowless houses and those in open-sided houses. On 4 farms, the investigation was carried out in different seasons, and 3 of them showed higher mean scores in winter than in summer. Next, observation of 2,255 birds from 15 flocks on 5 farms during a 4-week rearing period revealed that FPD was already produced at 7 days of age and worsened with age.     

Establishment of a quantitative ELISA for the measurement of allergen-specific IgE in dogs using anti-IgE antibody cross-reactive to mouse and dog IgE

As IgE plays a pivotal role in type I hypersensitivity-mediated allergic diseases, it is valuable to measure absolute quantity of serum antigen-specific IgE for clinical and research purposes. Here we describe a novel ELISA system that enables quantification of antigen-specific IgE in ng/ml in dogs. A newly developed monoclonal antibody (CRE-DM) was shown to recognize canine and mouse IgE equally in a dose dependent manner, but it did not recognize canine IgG. The reactivity of CRE-DM to canine IgE was also confirmed by an inhibition ELISA using canine IgE as an inhibitor and the maximum inhibition rate was 91.3%. In order to know whether canine IgE specific to an allergen could be quantitatively measured with an ELISA using CRE-DM, we established a quantitative ELISA that could measure canine IgE recognizing Cry j 1, one of the major allergens of Japanese cedar pollen. In this ELISA, a standard curve was created by using concentration-predetermined Cry j 1-specific monoclonal mouse IgE. According to the standard curve, the concentration of Cry j 1-specific IgE in dogs that were experimentally sensitized to Japanese cedar pollen could be calculated and determined in ng/ml. The specificity of the Cry j 1-specific IgE ELISA using CRE-DM was also confirmed by inhibition ELISA using canine IgE as an inhibitor and the inhibition rate was 97.0%. Reproducibility of the ELISA in three independent assays was determined using groups of pooled canine sera whose Cry j 1-IgE titers ranged from 155.9 to 888.2 ng/ml. Intra- and inter-assay reproducibility was determined with coefficient of variation ranging between 3.1-5.2% and 2.2-8.0%, respectively. These results demonstrated that the ELISA utilizing CRE-DM was a specific, reliable and robust new laboratory test that could quantify absolute amount of antigen-specific IgE in canine serum. The ELISA will serve as a useful tool in the clinics to evaluate the change of serum IgE titers during anti-allergic treatments as well as during seasonal fluctuation of allergen exposure.     

Plasma biochemical reference values in clinically healthy captive bearded dragons (Pogona vitticeps) and the effects of sex and season

Background: Bearded dragons are one of the most popular pet lizard species, and biochemical reference values are useful for health management of these reptiles. Objectives: The objectives of this study were to measure plasma biochemical values in healthy captive bearded dragons, determine reference values, and evaluate the effects of sex and season on the results. Methods: Blood samples were collected from 100 captive healthy bearded dragons in Tokyo during the summer and winter. Plasma biochemical measurements were performed using a dry‐slide automated biochemical analyzer. The data were then compared based on sex and season using 2‐way ANOVA. Results: Globulin, cholesterol, and calcium concentrations of females were higher in both summer and winter compared with the values obtained for males. Both males and females had higher uric acid concentrations in winter than in summer. When compared with males, females had a higher chloride concentration in summer and a higher total protein concentration and aspartate aminotransferase activity in winter. Potassium concentration in males was lower in winter than in summer, whereas in females cholesterol concentration was lower in winter than in summer. Conclusions: Biochemical values that differed based on sex and season in bearded dragons were similar to those in other lizards. These differences reflect physiologic differences in reproductive status in females and seasonal changes in temperature and hydration status. Plasma biochemical values established for bearded dragons in this study will be useful in the diagnostic assessment of captive animals.     

Multiple drainage can deliver higher rice yield and lower methane emission in paddy fields in An Giang Province,Vietnam

Paddy and Water Environment - The Mekong Delta produces 55% of Vietnam’s rice (Oryza sativa L.) through double or triple cropping each year. It accounts for a large proportion of...     

Verification of the prediction technique for the amount of adsorbed linuron on soils

The Freundlich isotherm was used to estimate the adsorption of the herbicide linuron on 39 Japanese soils to confirm the suitability of the prediction technique used in atrazine adsorption. Linuron was adsorbed on the soils according to the Freundlich isotherm. In conjunction with the log K _F prediction equation derived from the soil characteristics, it was possible to predict the linuron adsorption. Comparison of the equations for linuron with those for atrazine, indicated that the difference in the intercept of the 1 / n regression line against log K _F reflected the adsorptive characteristics of the herbicides.