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51.
Glutathione Peroxidase Activity,Plasma Total Antioxidant Capacity,and Urinary F2‐ Isoprostanes as Markers of Oxidative Stress in Anemic Dogs
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A. Kendall A. Woolcock A. Brooks G.E Moore 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2017,31(6):1700-1707
Background
Oxidative stress plays a role in the pathophysiology of several diseases and has been documented as a contributor to disease in both the human and veterinary literature. One at‐risk cell is the erythrocyte, however, the role of oxidative stress in anemia in dogs has not been widely investigated.Hypothesis/Objective
Anemic dogs will have an alteration in the activity of glutathione peroxidase (GPx), a decrease in of total antioxidant capacity (TAC), and an increased concentration of urinary 15‐F2‐isoprostanes (F2‐IsoP) when compared to healthy dogs.Animals
40 client‐owned dogs with anemia (PCV <30%) age‐matched to 40 client‐owned healthy control dogs.Methods
Prospective, cross‐sectional study. Whole blood GPx activity, plasma TAC, and urinary F2‐isoprostane concentrations were evaluated in each dog and compared between groups.Results
Anemic dogs had significantly lower GPx activity (43.1 × 103 +/‐ 1.6 × 103 U/L) than did dogs in the control group (75.8 × 103 +/‐ 2.0 × 103 U/L; P < 0.0001). The GPx activity in dogs with hemolysis (103 +/‐ 0.8 × 103 U/L) was not significantly different (P = 0.57) than in dogs with nonhemolytic anemia (43.5 × 103 +/‐ 1.1 × 103 U/L). The TAC concentrations (P = 0.15) and urinary F2‐isoprostanes (P = 0.73) did not significantly differ between groups.Conclusions and Clinical Importance
Glutathione peroxidase activity was significantly decreased in anemic dogs indicating oxidative stress. Additional studies are warranted to determine if antioxidant supplementation would improve survival and overall outcome as part of a therapeutic regimen for anemic dogs. 相似文献52.
53.
Koch TG Koch T Loretti AP Loretti A de Lahunta A Kendall A Russell D Bienzle D 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2005,19(3):367-372
A6 1/2‐month‐old Morgan filly was examined because of a history of abnormal behavior, teeth grinding, hypothermia, and electrolyte disturbances when weaned. She was from a breeding farm with several other Morgan horses. The 11‐year‐old dam had been purchased the year before as a proven broodmare, which had several previous foals. Breeding, gestation, and birth of this foal were normal. She was raised with 4 other mares and their offspring on pasture with free access to shelter in an open barn. Supplementary feeding consisted of oats and timothy hay. The owners reported that the foal showed unusual behavior, such as lack of apprehension of people, lack of distress from maternal separation, and a higher activity level than other foals of the same age. The foal extensively chewed the dam's tail and mane, masticated oats slowly with rapid jaw movements without actually swallowing them, and ground her teeth. She frequently nibbled the handler's clothes without biting, ate pebbles, and played with the salt block in the paddock. At 4 1/2 months of age, she was treated for suspected gastroduodenal ulcers and weaned. The referring veterinarian examined her 5 days after weaning because of dull demeanor and excessive teeth grinding. The foal was in thin body condition, hypothermic (37°C, 98.6°F), and tachycardic (60 beats per minute [bpm]) and had decreased borborygmi. Major abnormalities on serum biochemistry were severe hypernatremia (166 mmol/L; reference range 136–144 mmol/L) and hyperchloremia (128 mmol/L; reference range 94–104 mmol/L), azotemia (urea, 11.3 mmol/L; reference range 4.2–8.9 mmol/L), and hyperfibrinogenemia (5.2 g/L, reference range 1.6–2.9 g/L). The only abnormality on the CBC was hemoconcentration (PCV, 0.57 L/L; reference range 0.28–0.44 L/L). The foal was treated with penicillin procaine Ga (20,000 IU/kg [9072 IU/1b] IM q12h) and rifampinb (5 mg/kg [2.7 mg/1b] PO q8h). The next day the tachycardia worsened (120 bpm) and the foal was estimated to be 5–8% dehydrated. IV fluid therapy with lactated Ringer solutionc (LRS) was initiated, and the antibiotic was changed to ceftiofurd (2 mg/kg [0.91 mg/1b] IV q12h). The foal and dam were rejoined, and the foal's clinical status improved with resumption of nursing. Serial laboratory testing showed persistent hypernatremia 160 mmol/L) and hyperchloremia (123 mmol/L), azotemia urea 11.3 mmol/L and creatinine 168 umol/L; reference range 80–130 μmol/L), hyperglycemia (8.7 mmol/L; reference range 3.7–6.7 mmol/L), high aspartate aminotranferase activity (662 U/L; reference range 259–595 U/L), and high creatine kinase (CK) activity (1,196 U/L; reference range 108–430 U/L). The foal's condition improved and IV fluids were discontinued. Ceftiofur administration was discontinued and trimethoprim‐sulfamethoxazolee (25 mg/kg [11.3 mg/1b] PO q12h) was administered for 3 days. During the next month the foal was stable but the abnormal behavior persisted. She was weaned again, and within days marked behavior changes such as circling, throwing the head around compulsively, and severe hind‐end shivering recurred. At examination, the foal was dull, tachycardic (60 bpm), was hypothermic (33.6°C, 92.5°F), had dark red mucous membranes, and was estimated to be 5% dehydrated. Laboratory findings were similar to those of the previous tests except for high fibrinogen (7.1 g/L). The foal was again rejoined with the dam, treated with intramuscular penicillin, and referred 相似文献
54.
Kendall A Bröjer J Karlstam E Pringle J 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2008,22(5):1239-1242
BACKGROUND: Mycotic rhinitis is an uncommon disease in horses with a wide range of clinical signs and treatment options based on case studies of a single or only a few individuals. Moreover, while dogs with mycotic rhinitis appear to respond well to treatment with topical enilconazole, this approach has yet to be reported in horses. OBJECTIVES: The aim of this study was to describe the clinical findings, results of diagnostic investigations, treatment, and outcome in horses with mycotic rhinitis treated with topical enilconazole. ANIMALS: Eight horses were included in the study. METHODS: Medical records from 1995 to 2007 from an equine veterinary teaching hospital were reviewed to identify cases with a diagnosis of mycotic rhinitis. RESULTS: Eleven cases were identified, of which 8 were treated with topical enilconazole. Seven of those 8 horses recovered and had normal appearance of the nasal passages on endoscopic examination. Fungal culture, where positive, yielded only Aspergillus spp. Treatment was readily performed and no adverse effects were noted in these horses. In 5 horses, long-term follow-up revealed complete recovery for an average of 5 years. CONCLUSIONS AND CLINICAL IMPORTANCE: Mycotic rhinitis owing to Aspergillus spp. in horses can be effectively and safely treated with topical enilconazole. 相似文献
55.
PATHOLOGIC BASIS FOR RIM ENHANCEMENT OBSERVED IN COMPUTED TOMOGRAPHIC IMAGES OF FELINE NASOPHARYNGEAL POLYPS
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Christopher R. Lamb Kendall Sibbing Simon L. Priestnall 《Veterinary radiology & ultrasound》2016,57(2):130-136
In postcontrast computed tomographic (CT) images, feline nasopharyngeal polyps typically demonstrate enhancement of the peripheral rim. Computed tomographic images and histologic specimens of a case series of 22 cats with surgically removed nasopharyngeal polyps were reviewed retrospectively in an attempt to elucidate the origin of rim enhancement. Polyps were present in the tympanic cavity in 15 (68%) cats (three with extension into the nasopharynx), only in the nasopharynx in four (18%) cats, and only in the external ear canal in the remaining three (14%) cats. All polyps had variable degrees of epithelial injury. Hemorrhage and inflammatory infiltration were significantly more marked in the superficial stroma whereas edema was significantly more marked in the core stroma. In noncontrast CT images (n = 22), the tympanic bulla was thickened in all 15 cats with a polyp in the tympanic cavity and enlarged in eight (53%) of these cats. In postcontrast CT images (n = 15), an outer zone of relatively increased attenuation compatible with a rim was observed in 11 (73%) polyps. The magnitude and extent of rim enhancement in CT images was positively correlated with the histologic grade of inflammation in the superficial stroma and negatively correlated with the grade of edema in the superficial stroma. It appears that inflammation is the major determinant of contrast medium accumulation in feline nasopharyngeal polyps, and the tendency for inflammation to affect predominantly the superficial layers explains the frequent observation of a rim in postcontrast CT images. 相似文献
56.
57.
Hill Z Kirkwood B Kendall C Adjei E Arthur P Agyemang CT 《Public health nutrition》2007,10(8):827-833
OBJECTIVE: To identify regimen, individual, community and cultural factors that affect adoption and adherence to weekly vitamin A supplementation in Ghana. DESIGN: Fifty semi-structured interviews were conducted with women who would be eligible for vitamin A supplementation, 30 with husbands, and 13 with drug sellers, birth attendants and health workers. Six focus group discussions were also conducted with women. These interviews were followed by a 4-month capsule trial with 60 women. Data from a previously conducted communication channel survey of 332 women were also reviewed. SETTING: The study was conducted in Kintampo District in central Ghana. SUBJECTS: Participants for the semi-structured interviews and focus groups were selected from four villages and the district capital, and women in the capsule trial were selected at random from two villages. RESULTS: Knowledge of vitamins was low and taking 'medicines' for long periods and when healthy is a new concept. In spite of this, long-term supplementation will be accepted if motives are explained, specific questions answered and clear instructions are given. Potential barriers included the idea of 'doctor' medicines as curative, false expectations of the supplement, forgetting to take the supplement, losing the supplement, travelling, lack of motivation, perceived side-effects, concerns that the supplement is really family planning or will make delivery difficult, and concerns about taking the supplement with other 'doctor' or herbal medicine, or when pregnant or breast-feeding, or if childless. CONCLUSION: Successful supplementation programmes require appropriately designed information, education and communication strategies. Designing such strategies requires pre-programme formative research to uncover barriers and facilitators for supplementation. 相似文献
58.
Kendall AI 《Science (New York, N.Y.)》1931,74(1910):129-139
1. The isolation of a filter-passing diplococcus from the blood of certain cases of influenza by means of a special cultural medium is described. The experimental effects of this organism, while in the filterable state, upon rabbits, is discussed. 2. A procedure is formulated for inducing at will both a filterable and a non-filterable state in bacteria. Mention is made of a series of experiments in which both the filterable and the non-filterable state has thus been induced in a series of well-known bacteria comprising a variety of types. 3. It is postulated that a majority, if not all, known bacteria can and do exist in a filterable and in a non-filterable state. 4. A preliminary report of the isolation of microbes in the blood, not only of cases of influenza, but also from common cold, rheumatic fever, arthritis, from Staphylococcus bacteriophage and Besredka's Staphylococcus Antivirus is presented in evidence of the ubiquity of the procedure. 5. An explanation of the chemical basis for the existence of bacteria, both in the filterable and non-filterable states, in the animal and human body, and in culture, is proferred. 6. The relation of this chemical concept to microbic infection, and the state of microbes in the body during infection is discussed. In conclusion, it is a privilege as well as a pleasure to inscribe here my appreciation for the courteous cooperation of Dean Irving S. Cutter, Doctors Charles A. Elliott, Paul Starr, James G. Carr, Walter Nadler, Howard Alt and Herbert Barker, of the department of medicine, and to Northwestern University for the generous facilities and unrestricted opportunity for research which have contributed immeasurably to this investigation. 相似文献
59.
Kate L. Tsai Karen M. Vernau Kathryn Winger Danielle M. Zwueste Beverly K. Sturges Marguerite Knipe D. Colette Williams Kendall J. Anderson Jacquelyn M. Evans Ling T. Guo Leigh Anne Clark G. Diane Shelton 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2020,34(1):258-265