Flavonol Caffeoylglycosides as alpha-Glucosidase Inhibitors from Spiraea cantoniensis Flower

In the screening experiments for rat intestinal alpha-glucosidase inhibitors in 218 plants cultivated in the Japanese temperate region, potent maltase-inhibiting activity was found in the extract of flowers of Spiraea cantoniensis. The enzyme assay guided fractionation of the extract led to the isolation of three flavonol caffeoylglycosides, quercetin 3- O-(6- O-caffeoyl)-beta-galactoside ( 1), kaempferol 3- O-(6- O-caffeoyl)-beta-galactoside ( 2), and kaempferol 3- O-(6- O-caffeoyl)-beta-glucoside ( 3), as rat intestinal maltase inhibitors. This is the first report on the alpha-glucosidase-inhibitory activity of those flavonol caffeoylglycosides. Comparison in the activity of the isolates indicated the importance of caffeoyl substructures in the molecule for the alpha-glucosidase-inhibiting activity. The relatively high contents of the active isolates in the plant suggest that S. cantoniensis could be physiologically useful for treatment of diabetes.     

Experimental infection of enterotoxemic Escherichia coli associated with porcine edema disease and its pathologic characteristics in the intestine

Edema disease (ED) has become frequent in Japan, but no effective method for experimental infection has been developed. We report here the use of a capsule that resistant against gastric digestion to induce the ED in piglets. Four 21-day-old piglets were used. Shiga toxin 2e-producing Escherichia coli (STEC) cell pellet was encapsulated and administered orally. Two pigs received 1.0x10(10) CFU for two days, and the others received 3.9x10(8) CFU for three days. The high-dose group caused the typical clinical ED signs (palpebral edema or neurologic impairment). Eosinophil infiltration, swollen lymphoid follicles, and edema were observed in the ileum. The kidney had the thrombus in the glomerulus.     

Analysis of phylogenetic relationships in Mangifera by restriction site analysis of an amplified region of cpDNA

The phylogenetic relationship of 13 Mangifera species collected in Thailand were examined. Among these species, M. foetida, M. odorata, and M. sylvatica have been cultivated as fruit crops for local markets in Thailand, as well as M. indica (common mango). The other nine wild species also seem to have potential values as rootstocks and breeding materials for tolerance to critical environmental conditions. By restriction fragment length polymorphism (RFLP) analysis of a particular region of cpDNA, these species were classified into two groups. The first group consisted of M. indica and M. sylvatica, and the second group consisted of M. caloneura, M. cochinchinensis, M. collina, M. flava, M. foetida, M. gedebe, M. griffithii, M. macrocarpa, M. oblongifolia, M. odorata, and M. pentandra. The species belonging to the same group are monomorphic for 20 restriction enzymes tested. The present study indicated low diversity in the amplified region of cpDNA among Mangifera species tested, even though the species in the second group were scattered to different subgenera and sections.     

Sulfate and Nitrate Loads on a Forest Ecosystem in Kochi in Southwest of Japan

To assess the influence of acidic deposition on the forest ecosystem, it is necessary to evaluate the gross amount of acidic deposition. In this paper, we discuss the variation of sulfate (SO₄ ^2?) and nitrate (NO₃ ^?) loads as well as related concentration from 1991 to 1999 in the Hinoki (Chamaecyparis obtusa) plantation in Kochi, southwest Japan. The annual precipitation varied significantly from 1,700 to 3,900 mm during the study period. The annual sulfate concentration of rainfall was about 15 µmol L^?1, including about 80% non sea salt sulfate, while the annual nitrate concentration of rainfall was increased. The sulfate and nitrate concentrations of the through fall and the nitrate concentration of the stem flow were equal to or slightly higher than those of rainfall. However, the sulfate concentration of the stem flow was higher than that of rainfall, 21 to 55 µmol L^?1. The sulfate and nitrate loads of rainfall were measured to be 27 to 46 and 14 to 43 mmol m^?2 y^?1, respectively. The sulfate and nitrate loads of the through fall were the same or slightly higher than those of rainfall. In contrast, the sulfate and nitrate loads of the stem flow were less than those of rainfall. Combined sulfate loads of the through fall and the stem flow reached about 1.5 times that of the sulfate load of rainfall.     

Change in Physical Properties of Ferralsols with Tree Planting in the Central Amazon, Brazil

We examined the effects of mixed planting of fast growing tree species with indigenous Amazon tree species and soil tillage practices on soil physical properties in a deforested site used for agricultural cultivation. The site was located in the Central Amazon and consisted of Ferralsols. Bulk density and soil hardness were compared in the mixed planting site and an area where only indigenous tree species were planted. Many roots of the fast growing trees penetrated into the profile and caused changes in soil physical properties, i.e., decreasing bulk density and hardness. When tillage practices prior to planting were combined with mixed planting, the changes in soil physical properties were enhanced and the growth of not only the planted fast growing trees but also indigenous species such as mahogany, Swietenia macrophylla , which is shade tolerant, was accelerated.     

ROS-generating mitochondrial DNA mutations can regulate tumor cell metastasis

Mutations in mitochondrial DNA (mtDNA) occur at high frequency in human tumors, but whether these mutations alter tumor cell behavior has been unclear. We used cytoplasmic hybrid (cybrid) technology to replace the endogenous mtDNA in a mouse tumor cell line that was poorly metastatic with mtDNA from a cell line that was highly metastatic, and vice versa. Using assays of metastasis in mice, we found that the recipient tumor cells acquired the metastatic potential of the transferred mtDNA. The mtDNA conferring high metastatic potential contained G13997A and 13885insC mutations in the gene encoding NADH (reduced form of nicotinamide adenine dinucleotide) dehydrogenase subunit 6 (ND6). These mutations produced a deficiency in respiratory complex I activity and were associated with overproduction of reactive oxygen species (ROS). Pretreatment of the highly metastatic tumor cells with ROS scavengers suppressed their metastatic potential in mice. These results indicate that mtDNA mutations can contribute to tumor progression by enhancing the metastatic potential of tumor cells.     

Correlation between skeletal muscle fiber type and responses of a taste sensing system in various beef samples

The difference of muscle fiber type composition affects several parameters related to meat quality; however, the relationship between muscle fiber types and meat taste is unclear. To elucidate this relationship, we determined the taste of various beef samples using a taste sensor (INSENT SA402B) and analyzed its correlation with different muscle fiber type composition. We used 22 kinds of beef samples and measured nine tastes, including the relative and change of membrane potential caused by adsorption (CPA) values, using six sensors (GL1, CT0, CA0, AAE, C00, and AE1). The taste sensor analysis indicated positive value outputs for the relative C00, AAE, and GL1 values as well as for the CPA value of AAE, which corresponded to bitterness, umami, sweetness, and richness, respectively. We found significant positive correlations of the myosin heavy chain 1 (MyHC1) composition with umami taste, and with richness. This result suggests that high levels of slow MyHC1 can induce strong umami taste and richness in beef. We expect that our results will contribute to the elucidation of the relationship between muscle fiber types and meat palatability.     

Population Analysis of Fusarium graminearum from Wheat Fields in Eastern China

ABSTRACT Wheat heads showing symptoms of Fusarium head blight were collected from four commercial fields in Zhejiang Province, China, an area where epidemics occur regularly. A total of 225 isolates were subjected to population-level analyses using restriction fragment length polymorphism (RFLP) as markers. Diagnostic RFLP markers established that all isolates belonged to Fusarium graminearum lineage 6. Nine polymorphic probes were hybridized to all isolates, resulting in 65 multilocus RFLP haplotypes (MRH). Probing with the telomeric clone pNla17, which reveals differences among isolates in the hypervariable subtelomeric region, differentiated the 65 MRH further into 144 clones. Mean gene diversity for the four field populations was similar, ranging from H = 0.306 - 0.364 over the nine RFLP loci for clone-corrected data. High levels of gene flow were inferred from a low level of population subdivision among all field populations, indicating that they were part of the same population. Pairwise linkage disequilibrium measures did not unequivocally support a random mating population, because one-third of locus pairs were significantly different from the null hypothesis of no-association between alleles. We speculate therefore that sexual recombination may not be frequent and that high levels of genotypic diversity may be maintained by relatively low selection pressure acting on a highly diverse population.     

Changes in molecular species composition of triacylglycerol in subcutaneous fat from beef cattle during fattening

The composition of the molecular species of triacylglycerols (TG) in subcutaneous fat biopsied from Japanese Black steers was studied during the fattening period. An analysis of the fatty acid (FA) composition of the TG showed that palmitic, palmitoleic, stearic and oleic acids were the major FA, together accounting for over 80% of the total FA. The concentrations of C14:0, C16:0 and C18:0 decreased between 10 and 30 months of age, while those of C16:1 c9 and C18:1 c9 increased during the fattening period. The major molecular species among more than 40 compounds were palmitoyl‐dioleoyl‐glycerol (POO) and dipalmitoyl‐oleoyl‐glycerol (PPO). POO showed the highest concentration during the fattening period. The levels of palmitoyl‐oleoyl‐linoleoyl glycerol and some tentatively identified molecular species increased during the fattening period, while the levels of tripalmitoyl, dipalmitoyl‐stearoyl‐glycerol, palmitoyl‐distearoyl and palmitoyl‐stearoyl‐oleoyl‐glycerol decreased with growth. A comparison of the experimental values of TG molecular species with theoretical values derived from the experimental FA contents showed that the FA distribution in TG was non‐random. TG synthesis favors the formation of TG molecular species containing at least one C16 FA, rather than three C18 FA.     

Transport rates of surface materials on steep forested slopes induced by raindrop splash erosion

We used small (25 cm) sediment traps to evaluate the properties of surface material transport on steep slopes under humid temperate conditions in the Shikoku district of Japan. We sampled ten stands ofChamaecyparis obtusa (hinoki),Cryptomeria japonica (sugi),Pinus densiflora (red pine), and deciduous hardwood forest. Transport rates were estimated by dividing the amount of mass transported by the mass of rainfall during a given period. These rates varied widely among the ten stands: 0.0065–0.31 g m⁻¹ mm⁻¹ (a difference of two orders of magnitude) for fine earth, 0.00017–0.97 (four orders) for gravel, and 0.020–0.24 (one order) for litter. The transport rates in young hinoki stands with a low floor cover percentage (FCP) showed no seasonal changes. The transport rates changed by one or two orders of magnitude in hardwood forests with clear seasonal changes in FCP, as well as in juvenile and middle-aged hinoki and sugi stands, where understory floor cover increased and decreased seasonally. Rainfall intensity, however, showed no positive effects on transport rates, even in stands that had changing transport rates. The rates were mostly determined by forest type. We demonstrate that transport rate is an inherent property of a forest that reflects floor cover conditions and indicates the potential of surface material transport. This study was supported by the Research Council in the Ministry of Agricuture, Forestry and Fisheries, Japan.