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排序方式: 共有272条查询结果,搜索用时 15 毫秒
81.
Wataru FUJII Asuka ONUMA Shin YOSHIOKA Keisuke NAGASHIMA Koji SUGIURA Kunihiko NAITO 《The Journal of reproduction and development》2015,61(6):589-593
The generation efficiencies of mutation-induced mice when using engineered zinc-finger nucleases (ZFNs) have
been generally 10 to 20% of obtained pups in previous studies. The discovery of high-affinity DNA-binding
modules can contribute to the generation of various kinds of novel artificial chromatin-targeting tools, such
as zinc-finger acetyltransferases, zinc-finger histone kinases and so on, as well as improvement of reported
zinc-finger recombinases and zinc-finger methyltransferases. Here, we report a novel ZFN pair that has a
highly efficient mutation-induction ability in murine zygotes. The ZFN pair induced mutations in all obtained
mice in the target locus, exon 17 of aminopeptidase Q gene, and almost all of the pups had biallelic
mutations. This high efficiency was also shown in the plasmid DNA transfected in a cultured human cell line.
The induced mutations were inherited normally in the next generation. The zinc-finger modules of this ZFN pair
are expected to contribute to the development of novel ZF-attached chromatin-targeting tools. 相似文献
82.
Takuya HIROSE Takayuki OGURA Keisuke TANAKA Jun MINAGUCHI Takeshi YAMAUCHI Toshiyuki FUKADA Yoh-ichi KOYAMA Kazushige TAKEHANA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1385-1389
Ehlers-Danlos syndrome (EDS) is a group of disorders caused by abnormalities that are
identified in the extracellular matrix. Transforming growth factor-β1 (TGF-β1) plays a
crucial role in formation of the extracellular matrix. It has been reported that the loss
of function of zinc transporter ZRT/IRT-like protein 13 (ZIP13) causes the spondylocheiro
dysplastic form of EDS (SCD-EDS: OMIM 612350), in which dysregulation of the TGF-β1
signaling pathway is observed, although the relationship between the dermis abnormalities
and peripheral TGF-β1 level has been unclear. We investigated the characteristics of the
dermis of the Zip13-knockout (KO) mouse, an animal model for SCD-EDS.
Both the ratio of dermatan sulfate (DS) in glycosaminoglycan (GAG) components and the
amount of collagen were decreased, and there were very few collagen fibrils with diameters
of more than 150 nm in Zip13-KO mice dermis. We also found that the
TGF-β1 level was significantly higher in Zip13-KO mice serum. These
results suggest that collagen synthesis and collagen fibril fusion might be impaired in
Zip13-KO mice and that the possible decrease of decorin level by
reduction of the DS ratio probably caused an increase of free TGF-β1 in
Zip13-KO mice. In conclusion, skin fragility due to defective
ZIP13 protein may be attributable to impaired extracellular matrix
synthesis accompanied by abnormal peripheral TGF-β homeostasis. 相似文献
83.
Sakai Toru Omori Keisuke Oo Aung Naing Zaw Yan Naung 《Paddy and Water Environment》2021,19(2):283-294
Paddy and Water Environment - Summer rice cannot be grown near the coast of the Ayeyarwady Delta, Myanmar, because of the high salinity in river water during the dry season. This means that saline... 相似文献
84.
Paddy and Water Environment - Climate change is expected to exacerbate damage to agricultural production from natural disasters. Examination of measures to adapt to the damage represents an urgent... 相似文献
85.
Nagao K Okazaki R Nakamura T Miura YN Osawa T Bajo K Matsuda S Ebihara M Ireland TR Kitajima F Naraoka H Noguchi T Tsuchiyama A Yurimoto H Zolensky ME Uesugi M Shirai K Abe M Yada T Ishibashi Y Fujimura A Mukai T Ueno M Okada T Yoshikawa M Kawaguchi J 《Science (New York, N.Y.)》2011,333(6046):1128-1131
Noble gas isotopes were measured in three rocky grains from asteroid Itokawa to elucidate a history of irradiation from cosmic rays and solar wind on its surface. Large amounts of solar helium (He), neon (Ne), and argon (Ar) trapped in various depths in the grains were observed, which can be explained by multiple implantations of solar wind particles into the grains, combined with preferential He loss caused by frictional wear of space-weathered rims on the grains. Short residence time of less than 8 million years was implied for the grains by an estimate on cosmic-ray-produced (21)Ne. Our results suggest that Itokawa is continuously losing its surface materials into space at a rate of tens of centimeters per million years. The lifetime of Itokawa should be much shorter than the age of our solar system. 相似文献
86.
Ayato Kohzu Takahiro Tateishi Akiyoshi Yamada Keisuke Koba Eitaro Wada 《Soil Science and Plant Nutrition》2013,59(3):733-739
The 15N natural abundance in Pinus densiflora Sieb. et Zucco that had been inoculated and not inoculated with ectomycorrhizal fungi (Suillus granulatus (L.:Fr.) O. Kuntze) was compared. The inoculated pine needles showed a lower δ15N value, while the uninoculated ones showed a higher δ15N value. Higher δ15N values in the mycelial mat of the ectomycorrhizal fungi compared to those of the inoculated pine needles were also observed. These facts indicate that nitrogen isotope fractionation occurred during the nitrogen transport from mycorrhizal fungi to the host plants. 相似文献
87.
Hiroshi Masuda May Sann Aung Keisuke Maeda Takanori Kobayashi Naoki Takata Toru Taniguchi 《Soil Science and Plant Nutrition》2013,59(5):576-588
Iron (Fe) deficiency is a serious agricultural problem, especially in calcareous soils, which are distributed worldwide. Poplar trees are an important biomass plant, and overcoming Fe deficiency in poplars will increase biomass productivity worldwide. The poplar Fe-deficiency response and the genes involved in poplar Fe homeostasis remain largely unknown. To identify these genes and processes, we cultivated poplar plants under Fe-deficient conditions, both in calcareous soil and hydroponically, and analyzed their growth rates, leaf Soil and Plant Analyzer Development (SPAD) values, and metal concentrations. The data clearly showed that poplars have notable growth defects in both calcareous soil and a Fe-deficient hydroponic culture. They exhibited serious chlorosis of young leaves after 3 weeks of Fe-deficient hydroponic culture. The Fe concentrations in old leaves with high SPAD values were markedly lower in Fe-deficient poplars, suggesting that poplars may have good translocation capability from old to new leaves. The Zn concentration in new leaves increased in Fe-deficient poplars. The pH of the hydroponic solution decreased in the Fe-deficient culture compared to the Fe-sufficient culture. This finding shows that poplars may be able to adjust the pH of a culture solution to better take up Fe. We also analyzed the expression of Fe homeostasis-related genes in the roots and leaves of Fe-sufficient and Fe-deficient poplars. Our results demonstrate that PtIRT1, PtNAS2, PtFRO2, PtFRO5, and PtFIT were induced in Fe-deficient roots. PtYSL2 and PtNAS4 were induced in Fe-deficient leaves. PtYSL3 was induced in both Fe-deficient leaves and roots. These genes may be involved in the Fe uptake and/or translocation mechanisms in poplars under Fe-deficient conditions. Our results will increase a better understanding of the Fe-deficiency response of poplars and hence improve the breeding of Fe-deficiency-tolerant poplars for improved biomass production, the greening of high pH soils, and combatting global warming. 相似文献
88.
Sekiguchi Hiroyuki Masunaka Akira Nomiyama Koji Tomioka Keisuke 《Journal of General Plant Pathology》2021,87(5):326-329
Journal of General Plant Pathology - A postharvest rot of ripe sweet pepper fruits was found in Kagawa Prefecture in western Japan in October 2002. A fungus, isolated repeatedly from the diseased... 相似文献
89.
Takaaki Fujimoto Keisuke Kawakami Haruhisa Aimi Jun-ichi Shimizu Koichi Hasegawa Hikaru Kobori Satoru Tsuchikawa 《Journal of Wood Science》2013,59(5):383-388
This study examined the feasibility of near infrared spectroscopy as a novel technique for log assessment on the basis of wood property. Near infrared (NIR) spectra were obtained from the transverse section of green log and multivariate regression analysis was carried out to predict the stiffness of veneer processed from the log. The stiffness of the veneer was dynamic modulus of elasticity measured using ultrasonic method. The calibrations of veneer stiffness had moderate relationships between measured and NIR-predicted values, with regression coefficients ranging from 0.84 to 0.88. The calibration equations were applied to the test set and it was found that predictions were also well fitted, with regression coefficients ranging from 0.67 to 0.89. The results indicate that the variation of wood stiffness within the logs could be assessed using the NIR spectra from the cross-section of logs. The spectra were obtained from green condition of the log and the stiffness of veneer was measured after kiln drying. Thus, the results imply that the wood stiffness in dry condition could be predicted using the spectra collected from green logs. If the models obtained in this study put into the imaging system, the two-dimensional map of the stiffness would be visualized on the cross-section of logs. The NIR spectroscopy coupled with imaging system could compensate the weak point of the traditional methods for log assessment. 相似文献
90.
Maya OI Keisuke YAMADA Hiroyuki HAYAKAWA Hiroshi SUZUKI 《The Journal of reproduction and development》2013,59(1):92-96
Effective preselection of sex has been accomplished in several species of livestock and
also in humans using the flow cytometric sperm sorting method. A guaranteed high sorting
accuracy is a key prerequisite for the widespread use of sperm sexing. The standard
validation method is flow cytometric remeasurement of the DNA content of the sexed sperm.
Since this method relies on the same instrument that produced the original sperm
separation, it is not truly independent. Therefore, to be able to specifically produce
either male or female offspring in the dog, we developed a method of direct visualization
of sex chromosomes in a single sperm using fluorescence in situ
hybridization (FISH) as a validation method. Denaturation of canine spermatozoa by
immersion in 1 M NaOH for 4 min yielded consistent hybridization results with over 97%
hybridization efficiency and a good preservation of sperm morphology. There was no
significant difference between the theoretical ratio (50:50) and the observed ratio of X-
and Y-chromosome-bearing spermatozoa in any of the three dogs. In addition, the mean
purities of flow-sorted sex chromosomes in spermatozoa of the three dogs were 90.8% for
the X chromosome fraction and 89.6% for the Y chromosome fraction. This sorting was
evaluated by using the dual color FISH protocol. Therefore, our results demonstrated that
the FISH protocol worked reliably for both unsorted and sexed sperm samples. 相似文献