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101.
Sano J Oguma K Kano R Tsumagari S Hasegawa A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(1):103-105
Polymorphonuclear neutrophil (PMN) apoptosis was examined in three dogs with pyometra by TUNEL assay in a 24-hr incubation period and compared with that in healthy control dogs (n=5). The incidence of apoptotic PMNs in dogs with pyometra was 26.4 +/- 5% and that in healthy dogs was 54.3 +/- 7%. The results indicated that apoptotic PMN rates in dogs with pyometra were significantly lower than those in control dogs (p<0.05), suggesting the prolongation of PMN survival. 相似文献
102.
Maruyama H Oguma K Maeda S Kano R Tsujimoto H Watari T Tokuriki M Hasegawa A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(11):1423-1427
Thrombomodulin (TM) is a glycoprotein localized mainly on endothelial cell surfaces, and is a major regulator of vascular thromboresistance. The entire open reading frame of canine TM cDNA comprises 1737 bp, encoding 578 amino acid residues. Comparison of the deduced amino acid sequence from canine TM with those of human, mouse, rat, rabbit and bovine (partial) TM sequences revealed 73.1%, 69.1%, 65.8%, 74.3% and 69.5% identity, respectively. Canine TM mRNA expression was confirmed by RT-PCR analysis in lung, liver, spleen, kidney, pancreas and lymph node, and was relatively low in heart, cerebrum, urinary bladder and uterus. The present results provide valuable data for research into canine coagulation disorders. 相似文献
103.
Keisuke KAWASE Jumpei TOMIYASU Kazuyuki BAN Ryosuke ONO Satoshi ANDO Aya ONO Ran KIMURA Kanako TOMISAWA Motozumi MATSUI Shun-ichi SHIIHARA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(9):1454
Lions (Panthera leo) breed well under captivity, so contraception has been commonly conducted for population management, leading to a demand for a less invasive and reversible contraceptive approach in lions. In this study, we examined the efficacy of a commercial gonadotropin-releasing hormone vaccine as a method of suppressing reproductive activity in a sexually matured female lion. Under behavioral restraint, the vaccine was injected twice (days 0 and 109). After the initial vaccination, ovarian activity is still observed. After the second vaccination, contraceptive effect was confirmed for 246 days until restart of estrous cycles. We confirmed only a slight swelling around the injection site after the second vaccination. This study may suggest an alternative option for a contraceptive method in lions. 相似文献
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105.
Yuko Ito Kohei Matsushita Takuma Tsuchiya Yukari Kohara Tsuyoshi Yoshikawa Makoto Sato Keisuke Kitaura Satoshi Matsumoto 《Journal of toxicologic pathology》2014,27(1):91-95
This report describes a spontaneous nephroblastoma with lung metastasis in a 10-week-old male Crl:CD(SD) rat. Macroscopically, a white mass in the kidney and two white masses in the lung were observed. Histopathologically, the renal mass was located in the cortex of a kidney, and it caused pressure on the surrounding renal parenchyma. Three components could be distinguished in the tumor: blastemal, epithelial (primitive glomerular/tubular structures) and mesenchymal (neoplastic connective tissues) elements. Immunohistochemically, the tumor cells were positive for Wilms tumor 1 protein (WT1) and vimentin. Metastasis was found in the lung. Thus, the case was diagnosed as a nephroblastoma with lung metastasis. 相似文献
106.
Kazuhiro TAMURA Mikihiro YOSHIE Keisuke HASHIMOTO Eiichi TACHIKAWA 《The Journal of reproduction and development》2014,60(6):447-453
Angiogenesis in the developing corpus luteum (CL) is a prerequisite for establishment and maintenance of an early pregnancy. To explore the physiological significance of insulin-like growth factor-binding protein-7 (IGFBP7) in the developing CL, the effects of IGFBP7 on vascular endothelial growth factor (VEGFA)- and luteinizing hormone (LH)-induced in vitro tube formation were tested using isolated luteal microvascular endothelial cells (LECs). Capillary-like tube formation of LECs and their proliferation were stimulated by both VEGFA and LH. IGFBP7 treatment suppressed VEGFA- or LH-induced tube formation. The proliferation and migration of LECs, and phosphorylation of mitogen-activated protein kinase kinase and extracellular signal-regulated kinase 1/2 were inhibited by IGFBP7. Furthermore, IGFBP7 attenuated VEGFA-enhanced cyclooxygenase (COX)-2 mRNA expression and prostaglandin E2 secretion. These findings suggest the possibility that
luteal IGFBP7 secretion may suppress the stimulatory effect of VEGFA on angiogenesis in the early CL. 相似文献
107.
Motoki SASAKI Yoko AMANO Daisuke HAYAKAWA Toshio TSUBOTA Hajime ISHIKAWA Toshihiro MOGOE Seiji OHSUMI Masafumi TETSUKA Akio MIYAMOTO Yutaka FUKUI Teguh BUDIPITOJO Nobuo KITAMURA 《The Journal of reproduction and development》2014,60(1):62-67
In this study, we examined the existence and structure of areolae and the
steroidogenesis of areolar trophoblast cells in the Antarctic minke whale placenta
morphologically and immunohistochemically. Placentas were collected from the 15th, 16th
and 18th Japanese Whale Research Program under Special Permit in the Antarctic (JARPA) and
1st JARPA II organized by the Institute of Cetacean Research in Tokyo, Japan. The opening
and cavity of fetal areolae formed by taller columnar trophoblast cells (areolar
trophoblast cells) with long microvilli and a bright cytoplasm, as compared with the
trophoblast cells of the chorionic villi interdigitating with the endometrial crypts, were
recognized in observations of serial sections. The opening of the areolar cavity was
hidden by chorionic villi with areolar trophoblast cells. Furthermore, a closed pouch-like
structure lined by tall columnar cells similar to areolar trophoblast cells within the
stroma of chorionic villi was noticed and continued to the areolar cavity, with the
opening seen on serial sections. In a surface investigation of the chorion and endometrium
by SEM, maternal (endometrial) areolae irregularly surrounded by endometrial folds were
obvious. Moreover, we distinguished areolar trophoblast cells with long microvilli
attached with many blebs from trophoblast cells. In our immunohistochemical observations,
a steroidogenic enzyme, cytochrome P450 side chain cleavage enzyme (P450scc), was detected
with strong immunoreactivity in trophoblast cells. However, areolar trophoblast cells
showed weak or no immunoreactivity for P450scc. 相似文献
108.
109.
110.
Keisuke Maeda Fumika Yamamoto Masanari Toyoshi Masakazu Irie 《Animal Science Journal》2014,85(4):427-434
This study aimed to evaluate the effects of dietary lysine/protein ratio and fat levels on the growth, carcass characteristics and meat quality of finishing pigs fed feed made from food waste, including noodles and chocolate. Four dietary treatments, 2 levels of lysine/protein ratio (0.035 and 0.046) and 2 levels of fat (3.3% and 6.0%), were adapted to a 2 × 2 factorial arrangement. Each diet for the finishing pigs contained the same levels of adequate crude protein (16%) and lysine (0.58–0.75%), and similar levels of high total digestible nutrients (90.2–92.6%). In total, 32 LWD pigs with an average body weight of 57.2 kg were assigned to 4 dietary groups. The pigs were slaughtered at about 115 kg. Growth performance was not influenced by the dietary treatments. Carcass characteristics were slightly influenced by the dietary fat level. As the dietary lysine/protein ratio decreased, the marbling score of Longissimus dorsi muscle increased and the intramuscular fat (IMF) increased from 6.82% to 9.46%. Marbling score was not significantly influenced by the dietary fat level. These results indicate that IMF increased without adverse effects on growth, carcass characteristics and meat quality, when pigs were fed a diet with low lysine/protein ratio. 相似文献