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91.
Takaaki Fujimoto Keisuke Kawakami Haruhisa Aimi Jun-ichi Shimizu Koichi Hasegawa Hikaru Kobori Satoru Tsuchikawa 《Journal of Wood Science》2013,59(5):383-388
This study examined the feasibility of near infrared spectroscopy as a novel technique for log assessment on the basis of wood property. Near infrared (NIR) spectra were obtained from the transverse section of green log and multivariate regression analysis was carried out to predict the stiffness of veneer processed from the log. The stiffness of the veneer was dynamic modulus of elasticity measured using ultrasonic method. The calibrations of veneer stiffness had moderate relationships between measured and NIR-predicted values, with regression coefficients ranging from 0.84 to 0.88. The calibration equations were applied to the test set and it was found that predictions were also well fitted, with regression coefficients ranging from 0.67 to 0.89. The results indicate that the variation of wood stiffness within the logs could be assessed using the NIR spectra from the cross-section of logs. The spectra were obtained from green condition of the log and the stiffness of veneer was measured after kiln drying. Thus, the results imply that the wood stiffness in dry condition could be predicted using the spectra collected from green logs. If the models obtained in this study put into the imaging system, the two-dimensional map of the stiffness would be visualized on the cross-section of logs. The NIR spectroscopy coupled with imaging system could compensate the weak point of the traditional methods for log assessment. 相似文献
92.
Maya OI Keisuke YAMADA Hiroyuki HAYAKAWA Hiroshi SUZUKI 《The Journal of reproduction and development》2013,59(1):92-96
Effective preselection of sex has been accomplished in several species of livestock and
also in humans using the flow cytometric sperm sorting method. A guaranteed high sorting
accuracy is a key prerequisite for the widespread use of sperm sexing. The standard
validation method is flow cytometric remeasurement of the DNA content of the sexed sperm.
Since this method relies on the same instrument that produced the original sperm
separation, it is not truly independent. Therefore, to be able to specifically produce
either male or female offspring in the dog, we developed a method of direct visualization
of sex chromosomes in a single sperm using fluorescence in situ
hybridization (FISH) as a validation method. Denaturation of canine spermatozoa by
immersion in 1 M NaOH for 4 min yielded consistent hybridization results with over 97%
hybridization efficiency and a good preservation of sperm morphology. There was no
significant difference between the theoretical ratio (50:50) and the observed ratio of X-
and Y-chromosome-bearing spermatozoa in any of the three dogs. In addition, the mean
purities of flow-sorted sex chromosomes in spermatozoa of the three dogs were 90.8% for
the X chromosome fraction and 89.6% for the Y chromosome fraction. This sorting was
evaluated by using the dual color FISH protocol. Therefore, our results demonstrated that
the FISH protocol worked reliably for both unsorted and sexed sperm samples. 相似文献
93.
Kazuhiro UMEYAMA Kasumi HONDA Hitomi MATSUNARI Kazuaki NAKANO Tatsuro HIDAKA Keito SEKIGUCHI Hironori MOCHIZUKI Yasuhiro TAKEUCHI Tsukasa FUJIWARA Masahito WATANABE Masaki NAGAYA Hiroshi NAGASHIMA 《The Journal of reproduction and development》2013,59(6):599-603
Somatic cell nuclear transfer (SCNT) is a useful technique for creating pig strains
that model human diseases. However, production of numerous cloned disease model pigs
by SCNT for large-scale experiments is impractical due to its complexity and
inefficiency. In the present study, we aimed to establish an efficient procedure for
proliferating the diabetes model pig carrying the mutant human hepatocyte nuclear
factor-1α gene. A founder diabetes transgenic cloned pig was generated by SCNT and
treated with insulin to allow for normal growth to maturity, at which point
epididymal sperm could be collected for cryopreservation. In vitro
fertilization and intrafallopian insemination using the cryopreserved epididymal
sperm resulted in diabetes model transgenic offspring. These results suggest that
artificial reproductive technology using cryopreserved epididymal sperm could be a
practical option for proliferation of genetically modified disease model pigs. 相似文献
94.
Yasuyuki Tsukamasa Keisuke Kato Bimol Chandra Roy Yasunori Ishibashi Toru Kobayashi Tomohiro Itoh Masashi Ando 《Fisheries Science》2013,79(2):349-355
The color of the red muscle of pelagic fish is used as an indicator of freshness, and sustaining a bright red color is important for maintaining the commercial value of pelagic fish. Feeding fish with antioxidant compounds can delay metmyoglobin (metMb) formation, but the process requires long-term feeding. Live cultured Japanese horse mackerel Trachurus japonicus were used in this study. After anesthetization, 2 ml of blood was drawn from blood vessels, and a parenteral solution including an antioxidant compound was injected. Browning, metMb formation, and lipid oxidation of dark muscle during chilled storage were delayed by injecting sodium l-ascorbate as an antioxidant compound in the blood vessels of live fish. 相似文献
95.
Severe spotting, blight and drop of leaves caused by Colletotrichum dematium were found on potted plants of Polygonatum falcatum, a liliaceous ornamental, in open fields in Kagawa Prefecture, Japan, in May 2001. This new disease was named anthracnose
of P. falcatum.
Keisuke Tomioka, Jouji Moriwaki, Toyozo Sato contributed equally to this work.
The fungal isolate and its nucleotide sequence data obtained in this study were deposited in Genebank, National Institute
of Agrobiological Sciences and the DDBJ/EMBL/GenBank databases under accessions MAFF239500 and AB334523, respectively. 相似文献
96.
Toyozo Sato Yumi Iwamoto Keisuke Tomioka Satoshi Taba Atsushi Ooshiro Kazuko Takaesu 《Journal of General Plant Pathology》2008,74(1):91-93
Stem rot and wilt of Jew’s marrow (nalta jute, Corchorus olitorius) were found on Is. Okinawa, Okinawa Prefecture, Japan, in March 2000. An anamorphic fungus, Lasiodiplodia theobromae was isolated repeatedly from the diseased plants and demonstrated to cause the disease. We coined the Japanese name “kurogare-byô” of Jew’s marrow for the present disease because it was new to Japan, although it had already been reported in India and Bangladesh as black band of the plant. 相似文献
97.
Keisuke Tomioka Hiroyuki Sawada Takayuki Aoki Toyozo Sato 《Journal of General Plant Pathology》2008,74(5):405-407
Severe blight of stems, leaves and pods caused by Botrytis cinerea was found on pearl lupine (Lupinus mutabilis), a legume crop, grown in Kagawa Prefecture, Japan, in March–June 1996–2002. This disease was named “gray mold of pearl lupine”
as a new disease.
One of the fungal isolates obtained in this study was deposited in Genebank, National Institute of Agrobiological Sciences
as accession MAFF238557. 相似文献
98.
Takahiro Tezuka Keisuke Waki Kazutoshi Yashiro Maki Kuzuya Tomoko Ishikawa Yasumasa Takatsu Makoto Miyagi 《Euphytica》2009,168(2):177-188
Resistance to Fusarium oxysporum f.sp. melonis race 2 is conferred by a single dominant gene, Fom-1 in melon. Here, we identified DNA markers tightly linked to Fom-1 that could be used for marker assisted selection in breeding programs. First, we developed 125 F2 plants derived from the cross between melon lines P11 (fom-1fom-1) and MR-1 (Fom-1Fom-1). Using the F2 population, we constructed a linkage map including 14 SSR markers which had not been mapped previously. Fom-1 was confirmed to be allocated to linkage group 7. Then, we identified four AFLP markers using bulked segregant analysis.
The AFLP marker TAG/GCC-470 was completely linked to Fom-1 and other three markers were mapped near Fom-1. TAG/GCC-470 and TCG/GGT-400 were respectively converted to STS and CAPS markers. Usefulness of DNA markers was confirmed
in the analysis with several melon cultivars and lines. 相似文献
99.
M. Nazrul Islam Keisuke Ando Hidefumi Yamauchi Yoshinori Kobayashi Nobuaki Hattori 《Wood Science and Technology》2008,42(4):343-350
In this study, the ability of passive impregnation of Douglas fir (Pseudotsuga menziesii Franco) lumber was compared with a conventional full cell method. With an incising density of 10,000 holes/m2 carried out by CO2 laser, square Douglas fir lumber was treated by both passive impregnation and full cell method for 6 h and 50 min. Different
incising densities were also used in passive impregnation to examine the optimum incising density. It was observed that there
was no significant difference between these two methods regarding absorption of liquid, moisture content after dipping and
penetrated area both at cross and longitudinal sections. However, the value was higher in case of full cell method. Regarding
different incising densities, absorption and penetration of liquid was similar for 10,000 and 7,500 holes/m2 but it was low for 5,000 holes/m2. Therefore, it can be concluded that an incising density of 7,500 holes/m2 is the optimum for passive impregnation. The absorption of liquid and its distribution in wood indicates that this non-pressure
passive impregnation method can be a good preservation method for impermeable lumber and can be an alternative to full cell
method. 相似文献
100.
To investigate the influence of heating and drying history on the microstructure of dry wood, in addition to the dynamic viscoelastic
properties, CO2 adsorption onto dry wood at ice.water temperature (273 K) was measured, and the micropore size distribution was obtained
using the Horvath-Kawazoe (HK) method. Micropores smaller than 0.6 nm exist in the microstructures of dry wood, and they decreased
with elevating out-gassing temperature and increased again after rewetting and drying. Dry wood subjected to higher temperatures
showed larger dynamic elastic modulus (E′) and smaller loss modulus (E″). This is interpreted as the result of the modification at higher temperature of the instability caused by drying. Drying
history influenced the number of micropores smaller than 0.6 nm in dry wood not subjected to high temperature, although the
difference in the number of micropores resulting from the drying history decreased with increasing out-gassing temperature.
A larger number of micropores smaller than 0.6 nm exist in the microstructure of dry wood in more unstable states, corresponding
to smaller E′ and larger E″ than in the stable state. Consequently, unstable states are considered to result from the existence of temporary micropores
in the microstructures of dry wood, probably in lignin.
Part of this report was presented at the 55th Annual Meeting of the Japan Wood Research Society, Kyoto, March 2005, and at
the 56th Annual Meeting of the Japan Wood Research Society, Akita, August 2006 相似文献