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211.
Toyozo Sato Tadaoki Inaba Mitsutaka Mori Ken Watanabe Keisuke Tomioka Etsuji Hamaya 《Journal of General Plant Pathology》2005,71(2):127-132
Based on inoculation experiments and morphological studies on the pathogenic isolates of Plectosporium spp., Microdochium blight (Hakuhan-byo in Japanese) of pumpkin (Cucurbita maxima Duch.) occurring in Japan was reconfirmed to be caused by Plectosporium tabacinum, and seedling blight (Kabugare-byo in Japanese) of ranunculus (Ranunculus asiaticus L.) was demonstrated to be caused by P. tabacinum. Both diseases are renamed Plectosporium blight in this article. Some isolates of the fungus appeared to have host specificity, whereas the others had either weak or no pathogenicity to pumpkin and ranunculus. 相似文献
212.
Pedro PB Yokoyama E Zhu SE Yoshida N Valdez DM Tanaka M Edashige K Kasai M 《The Journal of reproduction and development》2005,51(2):235-246
To assess the permeability of mouse oocytes and embryos, matured oocytes and embryos at various stages of development were placed in five cryoprotectant solutions at 25 C for 25 min. From the cross-sectional areas of the oocytes/embryos, the relative change in volume was analyzed. In oocytes, shrinkage was least extensive and recovery was quickest in the propylene glycol solution, showing that propylene glycol permeates the oocytes most rapidly. Dimethyl sulfoxide, acetamide, and ethylene glycol permeated the oocytes slightly more slowly than propylene glycol. The oocytes in glycerol shrunk extensively and then expanded marginally, indicating slow permeation. The volume changes of 1-cell and 2-cell embryos were similar to those of oocytes, showing little change in permeability. In 8-cell embryos, the volume recovered much faster than in the earlier stages especially in glycerol and acetamide. In morulae, the volume recovery was much faster in glycerol and in ethylene glycol; in ethylene glycol, the extent of shrinkage was small and the recovery was fast, indicating an extremely rapid permeation. Although the permeability of oocytes/embryos generally increased as embryo development proceeded, the degree of increase varied greatly among the cryoprotectants. Interestingly, the volume change in propylene glycol was virtually unaffected by the stage of development. Such information will be valuable for determining a suitable protocol for the cryopreservation of oocytes/embryos at different stages of development. 相似文献
213.
Hiromitsu Furuya Keisuke Tubaki Tsutomu Matsumoto Shin-ichi Fuji Hideki Naito 《Journal of General Plant Pathology》2007,73(1):8-14
Roots of rice plants grown in paddy fields in a transplant culture system were collected seven times between 8 and 22 weeks
after transplanting, for two crop seasons. Rice seedlings grown in a potting medium amended with the collected roots were
significantly shorter than those grown in the same medium either without the addition of the roots or amended with pasteurized,
collected roots indicating that seedlings were inhibited by heat-labile microorganisms on the collected rice roots. Ninety-five
and 172 pure cultures of Pythium spp. and fungi, respectively, were isolated from the rice roots collected 5 or 7.5 weeks after transplanting in the fields.
Among these microorganisms, Pythium aristosporum inhibited seedling growth in greenhouse experiments, and Acremonium sp., Alternaria sp., Epicoccum nigrum, Fusarium sp., Massarina sp., Penicillium spp., Rhinocladiella sp., Stemphylium sp., Trichocladium sp., and several unidentified fungi inhibited seminal root growth in in vitro experiments. These microorganisms might be
involved in the inhibition of seedling growth in soils amended with the rice roots collected from the paddy field. Thus, roots
of rice plants at the middle stage of growth transplanted into paddy fields can harbor pathogenic or deleterious fungi or
Pythium sp(p). The effects of these microorganisms on rice growth in paddy fields are discussed. 相似文献
214.
Yasuda J Okada K Sato J Sato R Tachibana Y Takashima K Naito Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(1):87-89
A female Japanese Black cow showed hemorrhage under the mucous membrane of the eye conjunctiva and the vagina, and thrombocytopenia in peripheral blood. The clinical manifestations repeated spontaneously during long-term observation. The clinical findings were stable except for severe periodic bleeding. Prothrombin time, activated partial thromboplastin time and fibrinogen levels were normal. The anti-platelet antibody in the serum was analyzed by indirect platelet coagulation tests. Because the platelets in the micro-plate wells of the subject series showed coagulation up to 1:128, it was judged that anti-platelet antibody was present in the serum. A number of megakaryocytes were recognized in smear samples obtained from bone marrow of the scapula immediately after euthanasia. Therefore, this case was diagnosed as idiopathic thrombocytopenia. 相似文献
215.
Keisuke TOMIOKA Jun TAKEUCHI Toyozo SATO Tateo NAKANISHI 《Journal of General Plant Pathology》2002,68(1):103-104
Severe blight of stems and leaves caused by Rhizoctonia solani AG-1 (IB) was found on yacon (Smallanthus sonchifolius), a Compositae tuber crop, grown in Ehime Prefecture, Japan, from August to September 1996 and 1999. We named it “Rhizoctonia
blight of yacon” as a new disease.
Received 15 October 2001/ Accepted in revised form 25 November 2001 相似文献
216.
SALAMIAH Yukitaka FUKUMASA-NAKAI Hajime AKAMATSU Hiroshi OTANI Keisuke KOHMOTO Motoichiro KODAMA 《Journal of General Plant Pathology》2001,67(1):7-14
Several pathotypes of Alternaria alternata are known to produce host-specific toxins (HSTs) as agents of pathogenicity or virulence. However, investigations into the
genetic controls of HST biosynthesis and pathogenicity of Alternaria pathogens have been limited by the lack of a sexual stage in the life cycle of these pathogens. We report here the development
of a protoplast fusion system and its use for genetic analysis of HST production and specific pathogenicity of the tomato
pathotype of A. alternata that produces AAL-toxin as a HST. Drug-resistant transformants have been isolated by genetic transformation of nonpathogenic
A. alternata (strain O-94) and A. alternata tomato pathotype (strain As-27) with vectors conferring resistance to hygromycin B and geneticin, respectively. Protoplasts
of the respective transformants were fused by polyethylene glycol treatment or electrofusion. Fusion products were selected
by culturing in the presence of both hygromycin B and geneticin, then confirmed by amplification using a polymerase chain
reaction with specific primers to the transforming drug-resistance genes. Stable fusants were purified by successive subcultures
on selective medium and single-spore isolation. The resultant stable fusants, probably inter-strain hybrids, had the same
pathogenicity and toxin production as the wild-type strain As-27. These results suggest that protoplast fusion has potential
applications for genetic analysis of A. alternata pathogens.
Received 8 September 2000/ Accepted in revised form 27 October 2000 相似文献
217.
Keisuke SUGIMOTO Yoko FUJII Hiroshi SUNAHARA Takuma AOKI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(9):1101-1108
Hypertrophic cardiomyopathy (HCM) in cats is characterized by concentric left
ventricular (LV) hypertrophy and both diastolic and systolic dysfunction. Although
impaired cardiac function detected by tissue Doppler imaging (TDI) in cats with HCM was
previously reported, reference ranges of TDI in normal cats and cats with HCM have been
reported as widely variable. Two-dimensional speckle tracking echocardiography (STE) was
useful for assessment of cardiac function in human patients with HCM, but clinical utility
was not known in cats. The aim of this study was to assess global and segmental LV
myocardial function using STE in cats with HCM whose TDI variables were within the
reference range. A total of 35 cats of different breeds were enrolled in this study. The
HCM group (n=22) was cats diagnosed as HCM without left atrial enlargement and with normal
TDI measurements. HCM cats were further divided into a segmental hypertrophy (S-HCM) group
and a diffuse hypertrophy (D-HCM) group. The control group consisted of 13 clinically
healthy cats. No cats in any group showed any clinical symptoms. Conventional
echocardiography, TDI, and global and segmental STE indices were evaluated and compared
between groups. Only the longitudinal strain rate during early diastole was significantly
decreased in both HCM groups, even in all segments including those without hypertrophy in
S-HCM group. This study suggests that STE parameters are the more sensitive variables
compared with conventional TDI parameters to detect early myocardial diastolic dysfunction
in cats with HCM. 相似文献
218.
219.
Sung-Sik KANG Keisuke KOYAMA Weiping HUANG Yinghua YANG Yojiro YANAGAWA Yoshiyuki TAKAHASHI Masashi NAGANO 《The Journal of reproduction and development》2015,61(2):99-105
The present study aimed to establish an efficient system for bovine embryo production by in vitro fertilization (IVF) that can achieve stable normal fertilization and blastocyst developmental rates in any bull without optimization of the sperm concentration in IVF medium. We examined the effects of a PHE mixture (20 μM D-penicillamine, 10 μM hypotaurine and 1 μM epinephrine), theophylline (2.5 mM), and sperm concentration (1, 2 or 5 × 106 cells/ml) on fertilization and blastocyst developmental rates. High cleavage rates (78.3 to 92.4%) and blastocyst developmental rates (31.9 to 62.0%) at day 7 were obtained in the presence of PHE and theophylline in IVF medium with a sperm concentration of 2 × 106 cells/ml using sperm from 9 bulls. In addition, the synergistic effect of PHE and theophylline on normal fertilization (2 pronuclei) was clarified at 12 h after IVF with a sperm concentration of 1 × 106 cells/ml. Moreover, high
linearity, high flagellar beat cross frequency, and low amplitude of lateral head of motile sperm were found by computer-assisted sperm analysis. In conclusion, the combination of the PHE mixture and theophylline synergistically accelerates sperm motility and sperm penetration of bovine oocytes. Theophylline activates sperm motility with increasing intracellular cAMP. However, PHE prevents an excessive increase of cAMP and maintains sperm motility without hyperactivation. When the combination of PHE and theophylline is added to IVF medium at a sperm concentration of 2 × 106 cells/ml, we can achieve stable normal fertilization and blastocyst development in any bull. 相似文献
220.
Hiroshi SUNAHARA Yoko FUJII Keisuke SUGIMOTO Takuma AOKI Gou SUGAHARA Kinji SHIROTA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(1):95-99
A two-month-old female Chihuahua was diagnosed as severe pulmonary valvular
stenosis (PS). Although balloon valvuloplasty (BV) was successfully performed, restenosis
was observed 19 months after the procedure. Euthanasia was chosen due to low output
syndrome during the surgical repair attempted when the dog was 5 years old. Postmortem
examination revealed markedly thickened pulmonary valve due to the increase of
extracellular matrix which might be produced by increased α smooth muscle actin-positive
myofibroblasts. The thickening of the valve was associated with restriction of the valve’s
motion, resulting in restenosis in the present case. This is the first case report
documented histopathological and immunohistochemical findings of the restenotic pulmonary
valve in dogs with PS after BV. 相似文献