Severe blight of stems was found on Madagascar periwinkle (Catharanthus roseus) in Shizuoka and Okinawa prefectures, Japan, in July 2003 and September 2004, respectively. Similar fungi were isolated from the diseased plants. The isolated fungi caused the disease after inoculation and subsequently were reisolated from diseased tissues. The fungi were identified as species closely related to C. siamense or C. tropicale according to sequences of the β-tubulin-2 gene, indicating that they belong to the Colletotrichum gloeosporioides species complex. This is the first report specifying the genetic relationships of the pathogens of Madagascar periwinkle anthracnose in the complex. 相似文献
The purpose of this study was to assess the role of fleas for transmission of Bartonella species among wild rodents in Japan. Flea samples were collected from wild rodents and examined genetically for Bartonella infection. Bartonella DNA was detected from 16 of 40 (40.0%) flea samples. Sequence analysis demonstrated that 3 of 16 (18.8%) of the Bartonella-positive animals were infested with fleas from which the closely related Bartonella DNA sequence was detected, indicating that the fleas acquired Bartonella from the infested rodents. The DNA was detected in hemolymph, the midgut and the ovary (only in female), indicating that Bartonella might be colonized through the midgut and distributed into the body. 相似文献
Epigenetic alteration is an emerging paradigm underlying the long-term effects of chemicals on gene functions. Various chemicals, including organophosphate insecticides and heavy metals, have been detected in the human fetal environment. Epigenetics by DNA methylation and histone modifications, through dynamic chromatin remodeling, is a mechanism for genome stability and gene functions. To investigate whether such environmental chemicals may cause epigenetic alterations, we studied the effects of selected chemicals on morphological changes in heterochromatin and DNA methylation status in mouse ES cells (ESCs). Twenty-five chemicals, including organophosphate insecticides, heavy metals and their metabolites, were assessed for their effect on the epigenetic status of mouse ESCs by monitoring heterochromatin stained with 4¢,6-diamino-2-phenylindole (DAPI). The cells were surveyed after 48 or 96 h of exposure to the chemicals at the serum concentrations of cord blood. The candidates for epigenetic mutagens were examined for the effect on DNA methylation at genic regions. Of the 25 chemicals, five chemicals (diethyl phosphate (DEP), mercury (Hg), cotinine, selenium (Se) and octachlorodipropyl ether (S-421)) caused alterations in nuclear staining, suggesting that they affected heterochromatin conditions. Hg and Se caused aberrant DNA methylation at gene loci. Furthermore, DEP at 0.1 ppb caused irreversible heterochromatin changes in ESCs, and DEP-, Hg- and S-421-exposed cells also exhibited impaired formation of the embryoid body (EB), which is an in vitro model for early embryos. We established a system for assessment of epigenetic mutagens. We identified environmental chemicals that could have effects on the human fetus epigenetic status. 相似文献
In the last 40 years, the rice-cropping system has considerably changed in the Ayeyarwady Delta. The large archive of satellite imagery provides a history of how land and water resource managements have changed in the face of growing populations, resource demand, and climate change. This study aimed to assess the decadal changes in the rice-cropping system in the Ayeyarwady Delta by using the large archive of satellite imagery for the last 40 years (1981???2020). The long-term NDVI dataset provided various information on rice cultivation. Signal processing techniques were used to detect on the historical changes in the rice-cropping system, and the impact of climate change was assessed by using trend analysis. Until the 1980s, single-cropping of summer rice was dominant in the Delta. To enhance the grain yield of rice, the irrigation facilities were introduced in 1992 under an initiative of the Myanmar government. As a result, the annual cropping intensities increased from 1.087?±?0.390 in the 1980s to 1.422?±?0.499 in the 2010s. The information on historical change in the rice-cropping system would be useful to consider the practical and cost-effective utilization of remaining land and water resources. Moreover, the trend analysis of NDVI time-series showed negative trends in coastal areas. This indicates that the rice production in coastal areas has been constrained by the saline intrusion. The salt-affected areas are expected to expand under future climate change scenarios. Government support is highly required for sustainable rice production in the Delta.
In water-efficient rice production, grain yield is often constrained by panicle size. The objective of this study was to genetically dissect the response of panicle morphology to irrigation regimes in aerobic rice culture. We grew ‘Akihikari’ (a lowland japonica cultivar) × ‘IRAT109’ (an upland japonica cultivar) backcross inbred lines in aerobic soils with full or limited irrigation for 2 years, and examined 4 panicle traits—number of florets per panicle (FPP), number of primary branches per panicle (BPP), number of florets per primary branch (FPB), and frequency of pre-flowering floret abortion (%FA)—and grain yield. QTLs for BPP were detected in both the irrigation regimes but QTLs for FPB and %FA were detected mostly only in either of the irrigation regimes. The QTL for FPP on chromosome 2 (RM3421–RM213) coincided with that for yield under full irrigation, showing that this QTL is related to sink capacity and yield potential in aerobic rice culture. On the other hand, the QTL for FPB on chromosome 1 (RM3148–RM243) coincided with that for yield under limited irrigation, when water deficit was moderate. The QTL for root axis length at vegetative stage, previously identified in the same mapping population, was located near this region. This study unravelled the complicated genetic control on panicle morphology in aerobic rice culture, and suggested the positive roles of the dehydration avoidance mechanism by vigorous root growth on panicle size and yield under dry soil conditions. 相似文献
A fuzzy optimization model is developed to allocate allowable total nitrogen (T-N) loads to distributed nonpoint sources (NPSs)
and point sources (PSs) in a watershed for river water quality management using the linear programing technique. The watershed
is divided into uniform grid cells on which T-N loads issuing from NPSs such as paddy fields, upland crop fields and cities
are controlled. A geographic information system integrated with the digital elevation model facilitates computation of route
lengths of surface and subsurface flows from cells to a river running through the watershed. The T-N loads discharged from
their sources are assumed to decay, subject to distance-related first-order kinetics. As management goals, maximizations of
total allowable NPS loads, total allowable PS loads and total yield of rice are considered from environmental and economic
viewpoints. A prime constraint is an effluent limitation standard for the aggregate amount of loads that arrive at the downstream
end of the river. The fuzzy sets theory helps appropriately describe vague attitudes of decision-makers (i.e., stakeholders
and management authorities) in terms of constraints and conflicting goals. An application of the fuzzy optimization model,
developed as an improvement over our last nonfuzzy model, to a real watershed in Shiga prefecture, Japan, demonstrates that
the fuzzy model embodies our last model, and is capable of creating management alternatives for T-N load allocation in a more
practical and flexible manner. 相似文献
A cell-based distributed watershed model is developed which enables us to simulate the hydrological and hydraulic aspects
of the watershed in a refined fashion. With three-zoned cell profiling, the model is composed of three sub-models; tank model
for a surface water zone, soil moisture model for a surface soil zone, and unconfined shallow groundwater flow model for a
subsurface zone. Inclusion of the soil moisture sub-model modified to reroute the infiltration, routed from the tank sub-model,
into the return flow and the groundwater recharge features the model. The groundwater flow sub-model, numerically approximated
by use of the finite volume method and the implicit time-marching scheme, considers a network of on-farm drainage canals as
internal boundaries, which is an essential need for modeling the watershed including farmlands. Cascade-linking of the three
sub-models in a cell and assembling of all the cells over the entire watershed domain provides the global equations system
to be solved. Applicability of the model is demonstrated with its practical application to a real watershed in that paddy
and upland crop fields take great part of the land-use practice. It is then indicated in a quantified manner that rice farming
significantly contribute as a major groundwater recharger in an irrigation period to fostering and conservation of regional
water resources. Along with appropriately profiling a cell, the model is so versatile and tough that it can be applied without
difficulty to a watershed of diverse terrains and land-uses and the computations can stably be carried out. It is thus concluded
that the model presently developed could be a powerful “watershed simulator” to investigate and assess the time-varying hydro-environmental
properties of a watershed while separating and integrating the hydrological and hydraulic components of particular importance. 相似文献
Farmers in some parts of the Indochina Peninsula have been using earthen weirs to supply water to their rice fields, and the
use of such weirs is especially prevalent in Northeast Thailand. Although now a typical rain-fed rice cultivation area, a
significant percentage of rice fields in Northeast Thailand used earthen weirs before the extensive expansion of rain-fed
rice fields that occurred in the early 20th century. In this study, we clarified some of the historical changes associated
with the construction and use of earthen weirs and their effects on rice production. In particular, we investigated some of
the topographic effects, such as terrain, catchment size, and slope grade, on water delivery. In addition, water delivery
methods, construction periods, and the discontinued use of earthen weirs were examined with respect to regional influences
and topography. Earthen weirs were found to be most suitable in areas that exhibited complementarity between the riverbed
slope and the water catchment. The type of earthen weir was dependent on the magnitude of the riverbed slope. Earthen weirs
have been constructed continuously on steeper upstream slopes of rivers for more than a century, while weir construction on
lower riverbeds with larger catchments appears to be more recent.
The inner cellular structure of microconidia of Magnaporthe oryzae was examined using fluorescent probes and electron microscopic techniques. The volume of the nucleus relative to the cell
was significantly larger in microconidia than in macroconidia or vegetative hyphae, similar to observations for spermatia
of other fungi. Selective fluorescent staining revealed that cytosolic RNA was less abundant in microconidia than in macroconidia
and germ tubes, suggesting that general metabolic activity in microconidia is low. Consistently, GFP expression driven by
the TrpC promoter was highly active during the formation of phialides and microconidia but gradually decreased as the microconidia
matured. Such data suggest that microconidia are in a quiescent or dormant state. 相似文献