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91.
92.
The Otton frog, Babina subaspera, is an endangered forest frog species with unusual morphological and ecological features compared with other frogs. Eight microsatellite markers were isolated from B. subaspera and characterized with 24 individuals from Amami Islands, Kagoshima prefecture, Japan. The number of alleles observed for each locus ranged from 9 to 19 (average 13.9). The observed heterozygosity (H O) and the expected heterozygosity (H E) ranged from 0.71 to 0.92 and 0.83 to 0.92, respectively. These microsatellites will be useful for future genetic studies of B. subaspera, which will be essential in the conservation of this species. 相似文献
93.
Tran?Thi?Thanh Hien Tran?Ngoc?Hai Nguyen?Thanh?Phuong Hiroshi?Y.?Ogata Marcy?N.?WilderEmail author 《Fisheries Science》2005,71(2):279-286
The giant freshwater prawn, Macrobrachium rosenbergii, is cultured widely in the Mekong Delta region of Vietnam but it is often difficult or expensive for hatchery operators to
purchase commercial diets used as a feeding supplement to Artemia nauplii. Therefore, in the present study, the effects of lipid sources and lecithin on the growth and survival rate of M. rosenbergii larvae were examined in order to develop suitable hand-prepared larval diets for seed production of M. rosenbergii in this area. Six egg custard diets consisting of various ratios of lipid (originating from soybean oil and squid oil) and
lecithin were used for rearing Macrobrachium rosenbergii larvae. Treatments in which larvae were fed diets containing squid oil exhibited the highest body length and survival rates
(7.14–7.43 mm and 51.1–68.1%, respectively), and differed significantly from other treatments (P<0.05). Use of dietary soybean oil yielded the lowest body length and survival rates (6.29–6.75 mm and 22.0–48.7%), respectively).
The supplementation of dietary lecithin did not increase final body weight but did improve larval survival rates. The n-3
HUFA content of prawns fed dietary squid oil was higher than those of animals provided with other diets. These results indicated
that the most appropriate diet for rearing M. rosenbergii larvae is the diet containing 3% squid oil and 1.5% lecithin. 相似文献
94.
Takeshi?Sameshima Koichi?Kashimoto Keiko?Kida Yoshinori?Matsuda Teruo?Nonomura Koji?Kakutani Kengo?Nakata Shin-ichi?Kusakari Hideyoshi?ToyodaEmail author 《Journal of General Plant Pathology》2004,70(1):7-10
Leaves of tomato and barley were inoculated with conidia of Blumeria graminis f. sp. hordei race 1 (R1) or Oidium neolycopersici (KTP-01) to observe cytological responses in search of resistance to powdery mildew. Both conidia formed appressoria at similar rates on tomato or barley leaves, indicating that no resistance was expressed during the prepenetration stage of these fungi. On R1-inoculated tomato leaves, appressoria penetrated the papillae, but subsequent haustorium formation was inhibited by hypersensitive necrosis in the invaded epidermal cells. On the other hand, KTP-01 (pathogenic to tomato leaves) successfully developed functional haustoria in epidermal cells to elongate secondary hyphae, although the hyphal elongation from some conidia was later suppressed by delayed hypersensitive necrosis in some haustorium-harboring epidermal cells. Thus, the present study indicated that the resistance of tomato to powdery mildew fungi was associated with a hypersensitive response in invaded epidermal cells but not the prevention of fungal penetration through host papilla. 相似文献
95.
Oya S Inoue H Nakade T Ogata A Tamura M Kato S 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2003,50(5):230-234
Near-infrared spectroscopy (NIRS) that requires few compact instruments is a non-invasive and continuous method of monitoring haemodynamics in living organs. To assess the feasibility of using NIRS in evaluating the udder haemodynamics in six dairy cows, a probe component consisting of a light-emitting diode and a phototransistor was attached to the left side of the udder surface. Real-time variation of tissue haemodynamics was analysed and the data were transferred to the computer by way of a radiotelemetry system. On induction of temporary congestion by applying pressure with the hand pressed firmly against the left milk vein for 20 s, concentration of oxyhaemoglobin decreased throughout the 20-s duration of congestion, and concentration of deoxyhaemoglobin increased. A surge in blood volume was also observed from the beginning of congestion, then the volume attenuated to baseline. The data demonstrated that the reading seen on the monitor reflects the real-time status of the mammary gland. NIRS shows promise as a useful approach to physiological studies. 相似文献
96.
Shibahara T Akiba T Maeda T Ogata T Honda R Ishikawa Y Kadota K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(6):523-526
A 37-day-old male Japanese black calf showing marked salivation and leucocytosis died and was examined the tissues histologically. Histological lesions were characterized by severe focal necrotic glossitis on the ventral side of the root of the tongue. Immunohistochemically, Fusobacterium necrophorum subsp. necrophorum antigen was detected in the necrotic tissues and its distribution corresponded to that of the gram-negative, nonsporeforming, long filamentous organisms. Ultrastructural similarities between the organism and F. necrophorum subsp. necrophorum, but not subsp. funduliforme were observed. These findings clearly demonstrated that the fatal necrotic glossitis was caused by F. necrophorum subsp. necrophorum. This is the first report of bovine fatal necrotizing glossitis with leucocytosis caused by F. necrophorum subsp. necrophorum infection, and this organism may be an important fatal pathogen in calves with glossal lesions. 相似文献
97.
Nagayasu A Nakamura T Yamato O Tsuzuki K Hosaka Y Ueda H Tangkawattana P Takehana K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(9):881-886
GM1 gangliosidosis is one of the inherited metabolic lysosomal storage disorders characterized by neurological symptoms caused by beta-galactosidase deficiency and consequent accumulation of GM1 ganglioside in neuronal cells. Shiba dogs affected with GM1 gangliosidosis have been found to suffer from corneal opacity. In our morphological analysis, keratocyte enlargement was induced by abnormal intracellular accumulation of neutral carbohydrates, resulting in the loss of normal arrangement of collagen fibrils in the opaque cornea was found to be associated with the disorder. We therefore conclude that corneal opacity in this Shiba dog with GM1 gangliosidosis may be caused by neutral carbohydrate accumulation in lysosomes, swelling and dysfunction of keratocytes, and subsequent irregular arrangement of collagen fibrils in the corneal proper substance. 相似文献
98.
Isolation and serological grouping of ureaplasmas from dogs 总被引:1,自引:0,他引:1
99.
100.