全文获取类型
收费全文 | 201篇 |
免费 | 14篇 |
国内免费 | 1篇 |
专业分类
林业 | 36篇 |
农学 | 1篇 |
12篇 | |
综合类 | 15篇 |
农作物 | 7篇 |
水产渔业 | 17篇 |
畜牧兽医 | 113篇 |
园艺 | 1篇 |
植物保护 | 14篇 |
出版年
2022年 | 4篇 |
2021年 | 9篇 |
2020年 | 6篇 |
2019年 | 6篇 |
2018年 | 5篇 |
2017年 | 10篇 |
2016年 | 9篇 |
2015年 | 10篇 |
2014年 | 12篇 |
2013年 | 19篇 |
2012年 | 12篇 |
2011年 | 27篇 |
2010年 | 12篇 |
2009年 | 10篇 |
2008年 | 12篇 |
2007年 | 7篇 |
2006年 | 9篇 |
2005年 | 6篇 |
2004年 | 8篇 |
2003年 | 9篇 |
2002年 | 10篇 |
2001年 | 3篇 |
2000年 | 1篇 |
排序方式: 共有216条查询结果,搜索用时 62 毫秒
31.
Peng WANG Kousaku SOUMA Yuki KOBAYASHI Kei IWABUCHI Chihiro SATO Takayoshi MASUKO 《Animal Science Journal》2010,81(4):487-493
This study examined the differences between non‐inoculated (control) corn and Northern Leaf Blight (NLB)‐damaged corn (inoculated corn); dry matter (DM) yield, silage fermentation quality, nutritive value and feed intake by sheep were compared. Leaf, stem and grain dry weights and gross yield of inoculated corn were significantly (P < 0.05) decreased compared with control corn. The contents of water‐soluble carbohydrate and nitrogen‐free extract (NFE) were decreased in inoculated corn compared with control corn. Silage made from both inoculated and control corn showed good fermentation quality. The digestibility of DM, organic matter, ether extract, NFE, and energy of silage made from inoculated corn were significantly (P < 0.05) lower, and contents of total digestible nutrients (TDN) and digestible energy (DE) were also significantly (P < 0.05) lower compared with silage made from the control corn. DM intake showed no significant discrepancy between the two types of silage. TDN and DE intakes from inoculated silage were significantly (P < 0.05) lower compared with control silage. From the above results it was shown that NLB caused a decrease in DM yield and NFE content in corn and a decrease in the nutritive value and feed intake of silage. 相似文献
32.
33.
Shinohara T Ishii K Kanatsu-Shinohara M 《The Journal of reproduction and development》2011,57(2):288-295
Stem cells of the side population (SP) phenotype are found in many self-renewing tissues and can be identified by their unique ability to effectively exclude the dye Hoechst 33342. We previously established a method for expanding spermatogonial stem cells (SSCs) in vitro, but the frequency of SSCs is only about 1 to 2%, limiting detailed SSC analyses. In this study, we sought to isolate SSCs from in vitro cultures by exploiting their ability to exclude Hoechst 33342. In contrast to the findings of previous in vivo studies, we found that SP cells developed in a stochastic manner in vitro. Moreover, SP cells in culture were not enriched in SSCs, but they were interconvertible with non-SP cells. Although SP cells were consistently found in testes after transplantation of cultured cells, they were not enriched in SSCs. These results show that SSCs have an unstable SP phenotype and provide evidence that SSCs change their phenotype characteristics in response to their microenvironment. 相似文献
34.
Hayashi K Bhandal J Kim SY Rodriguez CO Entwistle R Naydan D Kapatkin A Stover SM 《Veterinary surgery : VS》2011,40(2):192-197
Objectives: To (1) describe vascular distribution in the grossly intact canine cranial cruciate ligament (CCL) using immunohistochemical techniques specific to 2 components of blood vessels (factor VIII for endothelial cells, laminin for basement membrane); and (2) compare the vascularity in different areas of interest (craniomedial versus caudolateral bands; core versus epiligamentous regions; and proximal versus middle versus distal portions) in the intact normal canine CCL. Study Design: In vitro study. Animals: Large, mature dogs (n=7) of breeds prone to CCL disease that were euthanatized for nonorthopedic conditions. Methods: Intact CCL were collected from fresh canine cadavers free from stifle pathology. CCL tissue was processed for immunohistochemistry and stained for factor VIII and laminin. Vascular density was determined by histomorphometric analysis. Results: Specific vascular staining was sparsely identified throughout the CCL; however, the proximal portion of the CCL appears to have a greater number of vessels than the middle or distal portion of the ligament. Conclusions: The CCL is a hypovascular tissue and its vascular distribution is not homogeneous. 相似文献
35.
Kei Takemura Hiroyuki Shingu Kentaro Ikuta Shigeru Sato Shiro Kushibiki 《Animal Science Journal》2020,91(1)
This study aimed to evaluate the effects of supplementing Saccharomyces cerevisiae (SC) during the pre‐ and post‐weaning periods on growth, metabolic and hormonal responses, and rumen fermentation in calves. Three‐week‐old Holstein calves were assigned to either control (n = 12) or SC group (n = 12), the latter of which received 2 × 109 cfu/day of SC. The experiment was conducted over a period of 7 weeks around weaning. Daily gain (DG) in the SC group was higher (p < .05) than that in the control group. In the SC group, plasma glucose, insulin, and growth hormone (GH) concentrations were higher (p < .05) and concentrations of glucagon and insulin‐like growth factor 1 (IGF‐1) tended to be higher (p < .1) than in the control group. Proportion of rumen propionate and concentration of rumen ammonia nitrogen at 10 weeks of age were greater (p < .05) in the SC group than that in the control group. Supplementation of SC around weaning may improve dietary nutrient and energy availability and increase plasma GH and IGF‐1 concentrations. These changes observed in SC‐supplemented calves could be closely related to the improvement of DG. 相似文献
36.
Takehiro Hachiken Kei Sato Takahiro Hasegawa Katsuyuki Ichitani Makoto Kawase Kenji Fukunaga 《Genetic Resources and Crop Evolution》2013,60(4):1559-1570
To elucidate diversity and evolution of the Waxy gene in foxtail millet, Setaria italica, we analyzed sequence polymorphism of Waxy gene in 83 foxtail millet landraces collected from various regions covering the entire geographical distribution of this millet in Europe and Asia. We found a unique geographic distribution pattern at the sequence level of gene haplotypes and also found a large diversity in East Asian landraces. We also found a higher degree of genetic polymorphism in a non-waxy phenotype than in other low amylose types, supporting the hypothesis that low amylose types recently originated from non-waxy type. 相似文献
37.
Toru Taniguchi Yasunori Ohmiya Manabu Kurita Miyoko Tsubomura Teiji Kondo Yong Woo Park Kei’ichi Baba Takahisa Hayashi 《Journal of Wood Science》2008,54(5):408-413
We performed biosafety assessments of transgenic poplars prior to field trials. Constitutive expression of the Aspergillus aculeatus xyloglucanase in Populus alba increased the cellulose content and specific gravity of its stem, the leaves of which were visibly greener, thicker, and
smaller than those of the wild-type plant. Although the young transgenic poplars grew faster than the wild type in a growth
chamber, there was no distinguishable difference in growth between the poplars when they were placed in a special screened
greenhouse. Allelopathic tests showed that the transgenic poplars do not produce harmful substances. Based on all the biosafety
assessments and the scientific literature on poplar species, we came to the conclusion that transgenic poplars probably do
not disturb the biological diversity of the surrounding environment, even when they are submitted to field trials. 相似文献
38.
39.
Kei YOSHIDA Toshio MATSUOKA Yui KOBATAKE Satoshi TAKASHIMA Naohito NISHII 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(2):275
The present study aimed to quantitatively evaluate muscle mass and gene expression in dogs with glucocorticoid-induced muscle atrophy. Five healthy beagles received oral prednisolone for 4 weeks (1 mg/kg/day), and muscle mass was then evaluated via computed tomography. Histological and gene expression analyses were performed using biopsy samples from the biceps femoris before and after prednisolone administration. The cross-sectional area of the third lumbar paraspinal and mid-femoral muscles significantly decreased after glucocorticoid administration (from 27.5 ± 1.9 to 22.6 ± 2.0 cm2 and from 55.1 ± 4.7 to 50.7 ± 4.1 cm2, respectively; P<0.01). The fast- and slow-twitch muscle fibers were both atrophied (from 2,779 ± 369 to 1,581 ± 207 μm2 and from 2,871 ± 211 to 1,971 ± 169 μm2, respectively; P<0.05). The expression of the growth factor receptor-bound protein 10 (GRB10) significantly increased after prednisolone administration (P<0.05). Because GRB10 suppresses insulin signaling and the subsequent mammalian target of rapamycin complex 1 activity, increased expression of GRB10 may have resulted in a decrease in protein anabolism. Taken together, 1 mg/kg/day oral prednisolone for 4 weeks induced significant muscle atrophy in dogs, and GRB10 might participate in the pathology of glucocorticoid-induced muscle atrophy in canines. 相似文献
40.
Effect of a single polymorphism in the Japanese quail NK‐lysin gene on antimicrobial activity
下载免费PDF全文
![点击此处可从《Animal Science Journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Taichiro Ishige Hiromi Hara Takashi Hirano Tomohiro Kono Kei Hanzawa 《Animal Science Journal》2016,87(1):143-146
NK‐lysins are cationic peptides that play important roles in host protection, and are an important constituent of innate immunity. We identified nine single‐nucleotide polymorphisms (SNPs) in the NK‐lysin open reading frame (ORF) from 32 Japanese quails in six strains: A, B, ND, K, P, and Y. The G to A substitution at nucleotide position 272 in the ORF resulted in a Gly (G) to Asp (D) amino acid substitution (Cj31G and Cj31D alleles). The Cj31D allele was detected in P (frequency 0.76) and Y (frequency 0.03) strains. We compared the antimicrobial activities of four synthetic peptides from the helix 2‐loop‐helix 3 region of avian NK‐lysins against Escherichia coli: Cj31G and Cj31D from quail and Gg29N and Gg29D from chicken. The antimicrobial activities of the four peptides decreased in the following order: Gg29N > Cj31G > Gg29D > Cj31D (P < 0.05). Although there were no differences in the predicted secondary structure of the Cj31G and Cj31D, the net charge of the Cj31G was higher than that of Cj31D. These data indicated that the antimicrobial activity of CjNKL is influenced by net charge, similar to that which has been observed in chicken. © 2015 Japanese Society of Animal Science 相似文献