Oral ingestion of collagen peptide causes change in width of the perimysium of the chicken iliotibialis lateralis muscle

Skeletal muscle is mainly composed of myofibers and intramuscular connective tissue. Bundles composed of many myofibers, with each myofiber sheathed in connective tissue called the endomysium, are packed in the perimysium, which occupies the vast bulk of the intramuscular connective tissue. The perimysium is a major determination factor for muscle texture. Some studies have reported that collagen peptide (Col-Pep) ingestion improves the connective tissue architecture, such as the tendon and dermis. The present study evaluated the effects of Col-Pep ingestion on the chicken iliotibialis lateralis (ITL) muscle. Chicks were allocated to three groups: the 0.15% or 0.3% Col-Pep groups and a control group. Col-Pep was administered by mixing in with commercial food. On day 49, the ITL muscles were analyzed by morphological observation and the textural property test. The width of the perimysium in the 0.3% Col-Pep group was significantly larger than other two groups. Although scanning electron microscopic observations did not reveal any differences in the architecture of the endomysium, elastic improvement of the ITL muscle was observed as suggested by an increase of the width of perimysium and improved rheological properties. Our results indicate that ingestion of Col-Pep improves the textural property of ITL muscle of chickens by changing structure of the perimysium.     

Estimation of yield and ultimate strengths of bolted timber joints by nonlinear analysis and yield theory

A finite element nonlinear analysis was conducted on bolted timber joints under lateral loads parallel and perpendicular to the grain. The results obtained from this analysis were compared with the experimental results and calculated values based on the yield theory. The analysis and experiment were performed on double shear bolted joints parallel and perpendicular to the grain with steel side plates and a slotted-in steel plate. It was found from the analysis that the yielding of wood and bolt occurred before the overall yielding of the bolted joint. Shear strength of bolted joints calculated from the yield theory using the embedding yield strength of wood and the yield moment of the bolt showed comparatively good agreement with the shear strength evaluated by 5% offset of the load–slip curve in the experiment and analysis. The shear strength of the bolted joint calculated from the yield theory using the embedding ultimate strength of wood and the ultimate moment of the bolt agreed quite well with the shear strength evaluated by the maximum load up to 15mm slip in the analysis. The former, parallel and perpendicular to the grain, were 11% and 34%, on average smaller than the latter in the experiment.Part of this paper was presented at the 52nd Annual Meeting of the Japan Wood Research Society, Gifu, April 2002; the Annual Meeting of Architectural Institute of Japan 2002, Ishikawa, August 2002; and the World Conference on Timber Engineering 2002, Shah Alam, Malaysia, August 2002     

Detection of novel kobu-like viruses in Japanese black cattle in Japan

During surveillance for bovine diarrhea of unknown causes in Japanese black cattle in Kagoshima Prefecture,Japan, we found two types of novel kobu-like viruses in fecal samples of calves. Sequence analyses revealedthat they had L protein and 2A protein with H-box/NC sequence motif, which are present in kobuviruses.Phylogenetic analysis revealed that they were related to kobuviruses; however, they clustered apart from otherkobuviruses. In the prevalence study of two types of novel kobu-like viruses, 16.9% and 10.4% prevalence ofthese viruses were observed in the feces of diarrheal calves in this area.     

Determination of embedding strength of wood for dowel-type fasteners

Embedding tests parallel and perpendicular to the grain were conducted to produce a database of embedding strength of wood for the design of dowel-type joints. Dowel diameters were 8,12,16, and 20mm. Embedding strength was evaluated by the 5% off-set method and a maximum load up to 5mm displacement according to EN383. The embedding strength parallel to the grain evaluated by the former method showed values close to those obtained with the latter method, but they showed a significant difference in tests conducted perpendicular to the grain. The embedding strength parallel to the grain was 0.9 times as large as the compressive strength parallel to the grain regardless of the evaluation method. The embedding strength perpendicular to the grain evaluated by the 5% off-set method was four times as large as the compressive strength perpendicular to the grain. When the embedding strength perpendicular to the grain was evaluated by a maximum load up to 5mm displacement according to EN383, the ratio of embedding strength perpendicular to the grain to the compressive strength perpendicular to the grain decreased as the dowel diameter increased.Part of this paper was presented at the annual meeting of the Architectural Institute of Japan 1999, Hiroshima, September 1999; the 50^th annual meeting of the Japan Wood Research Society, Kyoto, April 2000; and the World Conference on Timber Engineering 2000, Whistler, Canada, July–August, 2000     

Gustducin is expressed in the taste buds of the chicken

We investigated the expression of gustducin in chicken taste buds using molecular biological, biochemical and immunohistochemical techniques. Expression of a gustducin‐like sequence was detected by RT‐PCR in the tissues containing taste buds, and corresponded to the predicted gustducin gene in the chicken. Expression of this sequence was not detected in the brain, heart, liver, pancreas, intestine, kidney and testis of the chicken. The expressed sequence had a high specificity for oral tissues that contained taste buds. These results suggest that the detected sequence was the chicken gustducin gene. Next, we generated a polyclonal antiserum against the chicken gustducin protein to observe its localization in the oral tissues. The results revealed that the chicken gustducin was specifically expressed in the taste buds. It is suggested that the chicken has a gustatory system mediated by gustducin, and chicken gustducin is a reliable marker for taste buds or taste cells. This is the first molecular biological, biochemical and immunohistochemical demonstration of the presence of gustducin in the chicken.     

Digestive,fermentative, and physical properties of pineapple residue as a feed for cattle

The intake, digestion, and ruminal fermentation properties; the antioxidative activity; and the physical effectiveness of pineapple residue (PR) from the cut-fruit industry as a feed for cattle were investigated. Four nonlactating Holstein cows were fed a basal diet and diets with 1.4- and 3.6-kg dry matter (DM)/day of PR (low-PR and high-PR diets, respectively). The DM digestibility and total digestible nutrients were 71% and 70%, respectively. Ruminal pH, volatile fatty acids, and ammonia-N did not differ among the diets. The numbers of total viable, amylolytic, cellulolytic, and methanogenic bacteria and protozoa, and microbial protein synthesis in the rumen also did not differ significantly. Blood triglyceride, nonesterified fatty acids, urea nitrogen, and aspartate transaminase were lower in the high-PR diet. Feeding PR did not affect blood malondialdehyde, although PR has a higher antioxidative value than other commonly used feeds. The particle distribution of PR satisfied the recommended range for haylage. The chewing time for ingestion and rumination did not change with PR content, and the roughage value index of PR was 57-min/kg DM. These results suggest that PR has good intake and digestion properties and satisfactory physical effectiveness. Even a high-PR-content feed unlikely induces the risk of ruminal acidosis.     

The prevalence of antimicrobial-resistant Escherichia coli in two species of invasive alien mammals in Japan

The prevalence of antimicrobial resistance in 128 Escherichia coli isolates was investigated in two species of invasive alien mammals (IAMs): the small Asian mongoose (SAM) and Japanese weasel (JW). The SAM is found on the main island of Okinawa, Japan, where a large number of livestock is available, and the JW is present on a small island, where is isolated from the main island, and have a small number of livestock. We focused on the two IAMs, inhabiting under the different environments, and compared their prevalence of antimicrobial-resistant E. coli. In the comparison of the frequencies of antimicrobial-resistant E. coli isolates between the SAM and JW, JW showed significantly higher prevalence of resistance against three drugs, ampicillin, chlortetracycline and nalidixic acid, compared with SAM's test results (P<0.05). The bla(TEM) gene and the aph1 gene were detected in 35 subjects (91%) of ampicillin-resistant isolates and 6 subjects (100%) of kanamycin-resistant isolates, respectively. The tet (A) gene was detected in 62 subjects (46%) of CTC-resistant isolates, and the tet (B) gene was detected in 25 subjects (8%) of those in IAM. The present results suggest that some IAMs were the carrier of antimicrobial-resistant bacteria and their genes, and the frequencies of these resistances were different between two IAM species.     

Polyphenol composition of a functional fermented tea obtained by tea-rolling processing of green tea and loquat leaves

Phenolic constituents of a new functional fermented tea produced by tea-rolling processing of a mixture (9:1) of tea leaves and loquat leaves were examined in detail. The similarity of the phenolic composition to that of black tea was indicated by high-performance liquid chromatography comparison with other tea products. Twenty-five compounds, including three new catechin oxidation products, were isolated, and the structures of the new compounds were determined to be (2R)-2-hydroxy-3-(2,4,6-trihydroxyphenyl)-1-(3,4,5-trihydroxyphenyl)-1-propanone 2-O-gallate, dehydrotheasinensin H, and acetonyl theacitrin A by spectroscopic methods. In addition, theacitrinin A and theasinensin H were obtained for the first time from commercial tea products. Isolation of these new and known compounds confirms that reactions previously demonstrated by in vitro model experiments actually occur when fresh tea leaves are mechanically distorted and bruised during the production process.