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141.
Edamura K Itakura S Nasu K Iwami Y Ogawa H Sasaki N Ohgawara H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(5):549-556
Xenotransplantation of porcine pancreatic endocrine (PE) cells in a diffusion chamber, a bioartificial endocrine pancreas (Bio-AEP), was conducted to total pancreatectomized dogs. Six pancreatectomized dogs were divided into two groups of 3 dogs each. In three dogs of the control group, exogenous insulin was administered twice a day for 30 weeks to maintain fasting blood glucose (FBG) levels within the normal range. The remaining three dogs were implanted with Bio-AEPs (implantation group), in addition to daily insulin administration. In the implantation group, Bio-AEPs containing 1.3 to 1.8 x 10(7) cells per kg of body weight of the recipient were implanted without fixation into the abdominal cavity. In the control group, exogenous insulin requirements did not decrease during the experimental period, whereas it significantly decreased for a certain period (3, 11, 17 weeks) after implantation in all implanted dogs. In the implantation group, laparotomy was performed after FBG and the exogenous insulin requirement increased again and Bio-AEPs were removed. Two Bio-AEPs were completely destroyed, and the remaining one was encapsulated by thin fibrous tissue. In this dog, effusion was present within the capsule, but the Bio-AEP was not destroyed. Histopathologically, the necrosis, presumably caused by hypoxia, of the PE-cells was observed on transmission electron microscopy. In conclusion, Bio-AEP could function for a certain period after implantation in this study. However, more preclinical researches should be needed to apply this technique for the treatment of diabetic dogs. 相似文献
142.
143.
Cellulose isolated from wood is more susceptible than cotton cellulose to homogeneous hydrolysis in phosphoric acid. The influence of carboxyl group introduction at the C6 position on the hydrolysis rate of cellulose in 82.5% phosphoric acid was studied as a model of the oxidation of cellulose during pulping. The rate constant of hydrolysis for dissolving pulp was larger than that of cotton cellulose at temperatures of 25°–35°C. Mercerized cotton cellulose was partially oxidized regioselectively at the C6 position by a free radical system using 2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPO). The oxidized cellulose was obtained at a range of 1.7–12.7 mEq carboxyl content per 100 g of cellulose. The hydrolysis rate of the oxidized cellulose samples accelerated with increasing carboxyl content in the samples.Part of this work was presented at the 5th Annual Meeting of the Cellulose Society of Japan, Kyoto, July 1998 相似文献
144.
Factors influencing the rate of acetylation were examined based on the swelling of wood in the reaction solution and the dimensions of the wood sample. The activation energy of acetylation was also estimated. In a swelling test, it was found that wood swells thoroughly in acetic anhydride even without pyridine above 60°C. Therefore, pyridine may facilitate the acetylation process as a catalyst and not as a swelling agent. The weight gain, x (%), attained at reaction time t (h), for various compositions of acetylation solution or dimensions of wood sample were analyzed by applying an original rate equation [x = a × (1 – e–kt
)1/n
], where a is the ultimate weight gain (%), k is the rate constant (h–1), and n is a measure of the hindrance against the diffusion of reagent. The optimum volume fraction of pyridine in the pyridine-catalyzed acetylation was about 0.2. Accompanied by a rise in pyridine content, the reaction showed increased diffusion-controlled behavior. The rate constant, which is not affected by the dimensions of the wood sample, was estimated from which an activation energy of about 130kJ/mol was calculated. 相似文献
145.
Zhou Yu Iida Ikuho Minato Kazuya Kurosu Hiroshi 《中国林学(英文版)》2005,7(2):19-25
The adsorptive properties and selectivity of dyes and water molecules to cellulosic sheet, dependence of adsorptive properties of dyes on the concentration of dye solution, and discoloration of the dye solution due to the UV-ray irradiation were determined for 18 kinds of commercial dyes. The results are as follows: 1) the adsorptive properties of dyes to cellulose sheet differed greatly, but did not depend on the dye types such as acidic, basic and so on; 2) adsorptive properties of dyes to cellulosic sheet de- pended on the concentration of dye solution and were classified into 4 types: concentration-independent, increasing or decreasing with dye concentration, and having a maximum. This classification was irrelevant to the dye types; 3) the irradiation of UV-ray did not cause significant discoloration of dye solution itself, which suggested that wood components as well as dye molecules influence the discoloration of wood. 相似文献
146.
Takashi KOZASA Yuri ABE Kazuya MITSUHASHI Tomokazu TAMURA Hiroshi AOKI Masatoshi ISHIMARU Shigeyuki NAKAMURA Masatoshi OKAMATSU Hiroshi KIDA Yoshihiro SAKODA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(5):511-518
The Exaltation of Newcastle disease virus (END) phenomenon is induced by the
inhibition of type I interferon in pestivirus-infected cells in vitro,
via proteasomal degradation of cellular interferon regulatory factor (IRF)-3 with the
property of the viral autoprotease protein Npro. Reportedly, the amino acid
residues in the zinc-binding TRASH motif of Npro determine the difference in
characteristics between END-phenomenon-positive (END+) and
END-phenomenon-negative (END−) classical swine fever viruses (CSFVs). However,
the basic mechanism underlying this function in bovine viral diarrhea virus (BVDV) has not
been elucidated from the genomic differences between END+ and END−
viruses using reverse genetics till date. In the present study, comparison of complete
genome sequences of a pair of END+ and END− viruses isolated from
the same virus stock revealed that there were only four amino acid substitutions (D136G,
I2623V, D3148G and D3502Y) between two viruses. Based on these differences, viruses with
and without mutations at these positions were generated using reverse genetics. The END
assay, measurements of induced type I interferon and IRF-3 detection in cells infected
with these viruses revealed that the aspartic acid at position 136 in the zinc-binding
TRASH motif of Npro was required to inhibit the production of type I interferon
via the degradation of cellular IRF-3, consistently with CSFV. 相似文献
147.
Matsuda K Kousaka Y Nagamine N Tsunoda N Taniyama H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(7):763-765
A 20-year-old thoroughbred mare had a mass in the right kidney. The mass was encapsulated with fibrous capsule and composed of variably-sized papillary projections lined by a single layer of flattened to cuboidal neoplastic epithelial cells with no cytological and nuclear atypia. Immunohistochemically, the neoplastic cells were broadly positive for cytokeratin AE1/AE3 and granular staining for alpha-1-antitrypsin was focally detected; this immunohistochemical property was similar to that of the normal distal nephron. From these results, this case was diagnosed as papillary renal adenoma of distal nephron differentiation. 相似文献
148.
Kazuya Takai Tomoyuki Soejima Toshio Suzuki Kazuyoshi Kawazu 《Pest management science》2000,56(10):937-941
In order to develop an effective trunk‐injection agent against pine wood nematode, Bursaphelenchus xylophilus, an in vitro assay was used to examine the antinematodal activity of 58 commercially available compounds with known modes of action. Among compounds tested, the GABA receptor agonists had better anti‐nematodal activity than compounds influencing glutamate, N‐methyl‐D ‐aspartate, β‐adrenergic, dopamine, muscarinic acetylcholine and nicotinic acetylcholine receptors, as well as those inhibiting acetylcholinesterase, monoamine oxidase, 5‐hydroxytryptamine uptake and Ca2+, K+, Na+ and Cl− channels. Avermectins and milbemycins strongly inhibited propagation of the nematode. Emamectin benzoate proved to be the most active (IC95 0.050 µM ) being over 140 times more active than the active ingredient of conventional trunk‐injection agents. It is concluded that emamectin benzoate is a strong candidate for an anti‐nematodal trunk injection agent. © 2000 Society of Chemical Industry 相似文献
149.
Spruce wood blocks were acetylated in the presence of potassium acetate (KAc) at 20, 40, 60, 80 and 120°C. At 20°C, the weight percent gain (WPG) due to the KAc-catalyzed acetylation reached 20% in 18 days, whereas that due to pyridine-catalyzed acetylation did not exceed 8%. The hygroscopicity and dimensional stability of the KAc-acetylated wood were the same as those of conventionally acetylated wood at the same WPG, irrespective of reaction temperature. These facts suggest that the KAc enables simplified acetylation of wood at room temperature. The activation energy (E a) of the KAc-acetylation in the lower temperature range (20–40°C, 121–131 kJ/mol) was comparable to that of the acetylation of wood meal (140–146 kJ/mol). It was speculated that diffusion became a minor factor at reduced reaction rates in the lower temperature range, thus requiring a greater E a. 相似文献
150.