Morphological characterization of skin ganglion-like cells in Djungarian hamsters (Phodopus sungorus)

Characteristic ganglion-like cell proliferation observed in the skin of Djungarian hamsters was investigated using 24 male and 24 female hamsters, 1-6 months of age, to examine the anatomic location of these ganglion-like cells and their morphologic features. One abdominal skin tumor composed of these cells and resembling proliferative fasciitis in humans was also examined. Skin ganglion-like cells were rarely observed in young animals but increased in number and extent with age, especially in males. These cells were frequently seen in the ventral and medial regions of the trunk and legs rather than in the dorsal and lateral regions. Light microscopic examination of these ganglion-like cells revealed abundant vesicular basophilic cytoplasm with delicate intracytoplasmic silver stain-positive fibrils. Ultrastructurally, these cells contained abundant rough endoplasmic reticulum and Golgi complexes with dilated cisternae; intracellular collagen fibrils were present within these cisternae. Heat shock protein 47, beta-tubulin, and androgen receptor were expressed in these cells. The morphologic features of cells of one tumor resembling human proliferative fasciitis were identical to those observed in ganglion-like cells. The results of the present study suggest that these ganglion-like cells are derived from intrinsic undifferentiated mesenchymal cells in the dermis or subcutaneous adipose tissue and that any tumor-like lesion they form should be regarded as an abnormal proliferative lesion of skin ganglion-like cells rather than as proliferative fasciitis or fibroma.     

Cost sharing and sustainability of Pongsak Muang Fai irrigation system

The centuries-old, self-organizing Muang Fai community irrigation institution in northern Thailand has high potential in illustrating exemplary practices in irrigation cost sharing. This paper examines the cost sharing structure of the Pongsak Muang Fai Irrigation System in Mae Hong Son Province and its relationship with the system water management and sustainability. Results show that this primitive and high cost system accepts costs of the weir, the entire length of main ditch, not only the individually related ditch sections, and the management as common costs that should be equally shared so that it can gather sizeable membership to support the system. The capacity of this small scale run-of-the-river irrigation system with no river flow limitation is a simple summation of all determined farm intake capacities. The maintenance cost of the system every year depends on how much water should be diverted and conveyed, hence the use of “relative” scale of each farm intake capacity as the basis for sharing cost in the wet season paddy farming. Such structure is directly related to the water management under which every intake shall be served with continuous supply without permitting mid-canal water check-up and the members prefer to take collective action to keep enough water supply without leaving any room for suspicion of unfair water distribution. The direct relationship makes the members understand the purpose of payment and be willing to share the costs which are transparently estimated in easily understood terms and clearly de-aggregated into categories.     

Contextualizing farmers’ attitudes towards genetically modified crops

Analyses of the role of technological development in agriculture are central to an understanding of social change in agri-food systems. The objective of this paper is to contribute to the formation of a broader perspective of how farmers are positioning themselves with respect to controversial agricultural technologies through an empirical analysis of Washington State farmers’ willingness or unwillingness to try Genetically Modified Organisms (GMOs) technology on their farms. The use of this type of biotechnology in farming has been criticized for its potential harmful effects on natural environments and socio-cultural systems, while proponents highlight the possibilities for increasing production with minimal use of other inputs. An analysis of the extent of farmers’ expressed willingness to use GMOs provides an opportunity to better understand how their diverse thoughts about controversial agricultural technologies are shaped not only by their own experiences but also by social context. The present study does this by analyzing data from a farm survey conducted on a random sample of farmers from across Washington State. The results show that the production practices farmers utilize and the market strategies they employ may be at least as useful as farmers’ socio-economic characteristics in explaining what types of farmers appear to be more or less interested in potentially using this technology. Furthermore, the relationship between level of formal education and willingness to use GMOs is not straightforward. It may hide differences between farmers with respect to where and how they received their formal education as well as the type(s) of knowledge they gained. It is argued that future research should recognize the diversity that exists in farmers’ interests vis-á-vis particular technologies and should also explore how these interests are shaped by farmers’ past and present social networks and life experiences. Kazumi Kondoh is a PhD candidate in the Department of Sociology at Washington State University. Her research interests include environmental policies, science and technology, and sustainable agriculture. Raymond Jussaume is an Professor and Chair, Community and Rural Sociology at Washington State University. His primary areas of interest are sociology of agriculture, development sociology, and political sociology.     

Effects of beta-thujaplicin on anti-Malassezia pachydermatis remedy for canine otitis externa

The antifungal activity of beta-thujaplicin was evaluated against 51 Malassezia pachydermatis strains isolated from canine ear canals with or without otitis externa. For comparison, sensitivity tests were performed on M. pachydermatis isolates for nystatin, ketoconazole, and terbinafine HCl, all clinically available antifungal agents. The minimal inhibition concentrations over 50% of the tested isolates (MIC50) were 3.13 microg/ml for beta-thujaplicin and nystatin, 0.016 microg/ml for ketoconazole, and 1.56 microg/ml for terbinafine HCl. The antifungal effect for M. pachydermatis of beta-thujaplicin compared favorably with commercial antifungal agents. None of the 51 M. pachydermatis isolates showed resistance against any of the tested antibiotics investigated in this study. Ten representative isolates of M. pachydermatis were subcultured for 30 generations at concentrations close to the MIC levels of beta-thujaplicin, nystatin, ketoconazole, and terbinafine HCl, and examined to determine whether they had acquired resistance to each drug. As a result, M. pachydermatis was found to achieve resistance more easily for ketoconazole and terbinafine HCl than for beta-thujaplicin or nystatin. The MIC50 of beta-thujaplicin did not change during the course of subculture, and it is thought that the potential development of a resistant strain is low, even with continuous infusion for otitis externa therapy. beta-Thujaplicin is an inexpensive and safe treatment with anti-inflammatory and deodorant effects that can be recommended as an effective remedy for canine otitis externa.     

Population size estimation of the pond smelt Hypomesus nipponensis in Lake Kasumigaura and Lake Kitaura,Japan

We estimated the population size of the pond smelt Hypomesus nipponensis at the beginning of the fishing season in Lake Kasumigaura and Lake Kitaura, Japan using two DeLury methods and cohort analysis. A growth curve was estimated on the basis of monthly standard length. The relationship between standard length and weight was utilized for calculating the mean weight on the survey day in a particular month. Total monthly catches in Lake Kasumigaura and Lake Kitaura were calculated using partial monthly pond smelt catch obtained from processing plants located near the lakes between July and December. The total monthly catch was calculated from the total monthly catch weight and the monthly mean weight. The number of boats operating each month, compiled by the Kasumigaura-Kitaura Fisheries Office of Ibaraki Prefecture, was also employed. The estimated initial population sizes were compared with the population level index (PLI) estimated from survey data before the start of the fishing season. No significant differences were detected among the initial population sizes estimated by DeLury method, cohort analysis and PLI. The estimates ranged from 7.4 million to 410 million in Lake Kasumigaura, and from 7.7 million to 44 million in Lake Kitaura.     

Timing and extent of hypersensitive response are critical to restrict local and systemic spread of Pepper mild mottle virus in pepper containing the L 3 gene

Amino acid changes in Pepper mild mottle virus (PMMoV) coat protein (CP) that enhance, decrease, or nullify the resistance-inducing activity in Capsicum plants carrying the L ³ gene have been identified. In this study, molecular events underlying the L ³ -gene-mediated resistance were analyzed through the expression of hypersensitive response (HR)-related genes, HSR203J-Cc and HIN1-Cc, and defense-related genes, PR1-Cc and PR4b-Cc, upon infection with PMMoV CP mutants. The expression kinetics of the genes correlated with the degree of restriction of virus distribution in the inoculated leaves. The results suggest that the timing and extent of HR are critical factors to restrict virus spread both locally and systemically in L ³ -gene-mediated resistance.The nucleotide sequence data reported are available in the DDBJ/GenBank/EMBL databases under accession numbers AB162220 (HSR203J-Cc), AB162221 (HIN1-Cc), AB162222 (PR1-Cc), and AB162223 (PR4b-Cc)     

Practicable group testing method to evaluate weight/weight GMO content in maize grains

Because of the increasing use of maize hybrids with genetically modified (GM) stacked events, the established and commonly used bulk sample methods for PCR quantification of GM maize in non-GM maize are prone to overestimate the GM organism (GMO) content, compared to the actual weight/weight percentage of GM maize in the grain sample. As an alternative method, we designed and assessed a group testing strategy in which the GMO content is statistically evaluated based on qualitative analyses of multiple small pools, consisting of 20 maize kernels each. This approach enables the GMO content evaluation on a weight/weight basis, irrespective of the presence of stacked-event kernels. To enhance the method's user-friendliness in routine application, we devised an easy-to-use PCR-based qualitative analytical method comprising a sample preparation step in which 20 maize kernels are ground in a lysis buffer and a subsequent PCR assay in which the lysate is directly used as a DNA template. This method was validated in a multilaboratory collaborative trial.     

Predator–prey systems of drifting seaweed communities offthe Tohoku coast, northern Japan, as determined by feeding habitanalysis of phytal animals

ABSTRACT:   We collected phytal animals (fauna associated with drifting seaweeds)off the Tohoku coast in northern Japan in May and June 1994 to examine their feeding habits. Phytal animals were assignedto the following three feeding habit groups: (i) carnivores preyingon crustaceans: a gammarid ( Stenothoe sp.) and a decapodmegalops; (ii) omnivores feeding on crustaceans and pinnate diatoms:a cirripede ( Lepas anserifera ), three gammarids ( Calliopius sp., Jassaslatteryi and J. marmorata ), an isopod ( Idotea metallica ),a decapod crustacean ( Planes cyaneus ), a decapod megalopsand two caprellids ( Caprella penantis and Caprella mutica );and (iii) herbivores grazing on pinnate diatoms, fucoids and seaweedsother than fucoids: three gammarids ( Allorchestes angusta , Amphithoelacertosa and Perampithoe lindbergi ), an isopod ( Cymodocejaponica ) and a polychaete ( Platynereis bicanaliculata ).Pinnate diatoms in the gut contents were composed mainly of Licmophora spp., Navicula spp., Nitzschia spp.and Cocconeis spp. and seaweeds other than fucoids (i.e. Ectocarpussiliculosus and Sphacelaria furcigera ). The latter twoalgae seemed to be epiphytes on drifting seaweeds. These resultssuggest that phytal animals play an important role in drifting seaweed communitiesthrough trophic linkage with two primary producers (i.e. driftingseaweeds and the epiphytes growing on them).     

Relatedness structure estimated by microsatellites DNA and mitochondrial DNA polymerase chain reaction–restriction fragment length polymorphisms analyses in the wild population of kuruma prawn Penaeus japonicus

ABSTRACT: The genetic structure and variability of four wild populations of kuruma prawn in Japan were examined by estimating relatedness among individuals. The relatedness was estimated by five microsatellites (MS) DNA markers. Examination of relatedness showed that individuals were related significantly in Kumamoto and Kagoshima. In Kagoshima, some individuals showed full-sib level of relatedness. The analysis of mitochondrial (mt)DNA polymerase chain reaction–restriction fragment length polymorphisms (PCR-RFLP) was also performed, showing that the closely related individuals in Kagoshima tended to share a common haplotype. It is, therefore, supposed that there are many kins in Kumamoto and Kagoshima. However, the heterozygosities and allelic and genotypic frequencies in MS-DNA analysis were not significantly different among the localities. Moreover, the haplotype distributions of mtDNA in Kumamoto and Kagoshima were significantly different from other localities. Thus, it is suggested that no spatial differentiations occurred due to the geographic or historical effects between the localities and that there is the possibility of a mixture of hatchery populations in Kumamoto and Kagoshima.