The effects of concentrations of lysine in media on differentiation of 3T3‐L1 preadipocytes

Intramuscular fat content is increased by feeding of low lysine diets in pigs. Reduction in dietary lysine intake results in low plasma lysine concentration and low cytosolic lysine concentration in skeletal muscles. From these observations, we hypothesized that low plasma lysine concentration in pigs fed on low lysine diets reduced supply of lysine from blood circulation to preadipocytes, and this limited supply of lysine might promote adipocyte differentiation in porcine muscles. In order to verify the hypothesis, we investigated the effects of low concentrations of lysine in culture medium on differentiation of 3T3‐L1 preadipocytes. Low concentration of lysine suppressed lipid accumulation and messenger RNA (mRNA) expression and enzyme activity of fatty acid synthase. mRNA expressions of peroxisome proliferator‐activated receptor γ (PPARγ) and CCAAT/enhancer binding protein α (C/EBPα) were lower in cells cultured in low lysine medium. On the other hand, mRNA and protein expressions of C/EBPβ and C/EBPδ were not inhibited by low concentrations of lysine in culture medium. These results indicate that low lysine concentrations in culture medium inhibit differentiation of 3T3‐L1 preadipocytes through inhibiting the mRNA expressions of PPARγ and C/EBPα.     

Spatial variability of throughfall under a single tree: Experimental study of rainfall amount, raindrops, and kinetic energy

To evaluate the spatial variability of throughfall amount, raindrops, and erosivity under a single canopy during calm meteorological conditions, indoor experiments were conducted using a 9.8-m-tall transplanted Japanese cypress (Chamaecyparis obtusa) and a large-scale rainfall simulator. Drop size distribution, drop velocity, and kinetic energy of throughfall varied spatially under a single canopy as did throughfall amount and rain rate. Compared with throughfall rain rate, the variability was similar in drop size distribution, lower in drop velocity, and higher in kinetic energy. The results suggest that the spatial distribution of throughfall amount was dominated by the canopy shape and position of branches inside the canopy, and thus the spatial distribution was correlated with the radial distance from the trunk. Throughfall amount and rate were lower at the midway point between the trunk and the canopy edge. Throughfall drop size indices (drop size distribution, drop velocity, and unit kinetic energy) varied spatially while did not differ significantly. On the other hand, time-specific throughfall kinetic energy was correlated with the radial distance from the trunk. The dependence the throughfall kinetic energy on the radial distance from the trunk was dominated by the spatial distribution of throughfall amount. The trend in the spatial distribution of throughfall revealed in this study will aid in modelling canopy water processes and in predicting soil erosion on the bare forest floor.     

Soil Acidification and Decline of Trees in Forests Within the Precincts of Shrines in Kyoto (Japan)

The historical Japanese city of Kyoto boasts a great many old Buddhist temples and Shinto shrines, many of which are surrounded by sizable forests that have long been preserved as sacred forests. However, acidic deposition has been fallen on the forests in Kyoto for many years. For this study, we conducted soil surveys and investigated the extent of decline of the trees in two Shinto shrines as historic monuments of ancient Kyoto. Our study revealed clear decline in two key tree species (Cryptomeria japonica (Japanese cedar) and Chamaecyparis obtusa (Japanese cypress)) in both shrines, with some trees showing signs of mortality. The soil was acidic, with an average pH of 4.35. Nutrient salt content too was only about one tenth the national average, with exchangeable Ca (0.52 cequiv./kg) and Mg (0.23 cequiv./kg) for 0?C20 cm surface soil. The (Ca+Mg+K)/Al molar ratios were also very low, with 80% of all soil samples having a ratio of 10 or below. Such soil conditions are thought to hamper the sound growth of both Japanese cedar and Japanese cypress, and soil acidification is one of the most likely causes of the decline of temple and shrine forests in Kyoto.     

METABOLITE PROFILING OF SHOOT EXTRACTS,ROOT EXTRACTS,AND ROOT EXUDATES OF RICE PLANT UNDER PHOSPHORUS DEFICIENCY

We applied a metabolite profiling technique to investigate root exudates under phosphorus (P) deficiency. Oryza sativa was grown in culture solution containing three P levels (0, 1, and 8 mg P L^?1). Shoot extracts, root extracts, and root exudates were obtained from 18 and 23-day-old plants and their metabolites were determined by capillary electrophoresis/time-of-flight mass spectrometry. Eighty, 90, and 65 metabolites were identified in shoot extracts, root extracts, and root exudates, respectively. Sixty-three to eighty-four percent of the metabolites were exuded to the rhizosphere. More than 33% of the metabolites in the root exudates showed higher concentration at low P than at high P. On the other hand, only 14% of the metabolites in the root extracts showed lower concentration at low P than at high P. These results suggest that rice roots actively release many metabolites in response to P deficiency.     

Elemental affinity for siderite found in a Japanese paddy subsoil

ABSTRACT

Siderite (FeCO₃), a type of carbonate mineral, is very occasionally recognized as a nodule in anoxic soils and sediments. During siderite formation, elements are expected to be accumulated or excluded between siderite and bulk soil. Therefore, we verified the affinity of 40 elements for siderite found in the gley horizon of a smectite-rich paddy field in northeastern Japan from the elemental concentration of the soil and the siderite dissolved in 2.88 mol L^?1 hydrochloric acid (10% HCl). The concentration ratio of the target element of the soil to the siderite was expressed by the product of the dimensionless R₀ value and ratio of titanium (Ti) concentration of the soil to the siderite (C_siderite /C_soil) = R₀ (Ti_siderite /Ti_soil). The affinity of each element for the siderite was analyzed by comparing the R₀ values. In comparison with Ti used as the reference element, P, Cr, Co and Mo were accumulated (R₀ > 1.0), and alkali metals, such as Li, Na and K, and chalcophile elements, such as Cu, Zn, Cd and Pb, tended to be equal or excluded from the siderite (R₀ ≦1.0). Of the group 2 elements, Ca, Sr and Ba were accumulated, while Mg was excluded. The affinity of Mn for siderite exceeded that of Fe as the main component of the siderite, which reflects the fact that siderite was formed in a fresh water condition. The siderite size did not influence the elemental affinity. Observation of micromorphology using a scanning electron microscope showed that the siderite nodules were aggregations of siderite particles and clays, suggesting that the siderite grew by taking in clay around it. The rare earth element (REE) distribution pattern showed a tendency not to increase linearly but to increase with forming a concave curve at the intervals of the four elements with the increase of atomic number (i.e. tetrad effects). Although these findings are from the lower horizons of anoxic soils, the elemental affinity for siderite of this study is useful in order to elucidate the complex elemental dynamics in the anoxic environment.     

Large-area three-dimensional molecular ordering of a polymer brush by one-step processing

Rational molecular design and processing, enabling large-area molecular ordering, are important for creating high-performance organic materials and devices. We show that, upon one-step hot-pressing with uniaxially stretched Teflon sheets, a polymer brush carrying azobenzene-containing mesogenic side chains self-assembles into a freestanding film, where the polymer backbone aligns homeotropically to the film plane and the side chains align horizontally. Such an ordered structure forms through translation of a one-dimensional molecular order of the Teflon sheet and propagates from the interface macroscopically on both sides of the film. The resultant wide-area bimorph configuration allows the polymer film to bend rapidly and reversibly when the azobenzene units are photoisomerized. The combination of polymer brushes with hot-pressing and Teflon sheets provides many possibilities in designing functional soft materials.     

GnRHa-induced spawning of wild-caught jack mackerel Trachurus japonicus

The jack mackerel Trachurus japonicus is a key commercially exploited fish species in Japan. The rearing experiment often provides information that is useful for understanding the reproductive characteristics of wild stocks; however, there has been no study on spawning in captive T. japonicus. In the study reported here, we induced spawning in T. japonicus caught in the wild by hook and line. Females with fully vitellogenic oocytes and males during spermiation were selected by gonadal biopsy and injected with gonadotropin-releasing hormone analog (GnRHa) mixed in molten coconut butter. This treatment was performed four times in different groups of four females and five to eight males, and each group was maintained in a 3-m³ concrete tank. We observed the first spawning at 1 or 2 days post-injection and collected between 41,690 and 149,450 eggs. Spawning was recorded on 18 consecutive days in one experiment and for 3 days continuously in the other experiments. In the former, spawning ended when the water temperature reached 23 °C and occurred mainly between 2100 and 2400 hours. These results indicate that GnRHa-induced spawning may be useful for evaluating the reproductive characteristics of T. japonicus and obtaining fertilized eggs to conduct larval experiments.     

A New Dibenz[b,e]oxepine Derivative, 1-Hydroxy-10-methoxy-dibenz[b,e]oxepin-6,11-dione,from a Marine-Derived Fungus,Beauveria bassiana TPU942

1-Hydroxy-10-methoxy-dibenz[b, e]oxepin-6,11-dione (1) was obtained from the culture broth of a marine-derived fungus, Beauveria bassiana TPU942, isolated from a marine sponge collected at Iriomote Island in Okinawa, together with two known compounds, chrysazin (2) and globosuxanthone A (3). The structure of 1 was elucidated on the basis of its spectroscopic data (HREIMS, 1D and 2D NMR experiments including ¹H–¹H COSY, HMQC and HMBC spectra). Dibenz[b, e]oxepines are rare in nature, and only six natural products have been reported. Therefore, compound 1 is the seventh natural product in this class. Compounds 2 and 3 showed an antifungal activity against Candida albicans, and 3 inhibited the cell growth against two human cancer cell lines, HCT-15 (colon) and Jurkat (T-cell lymphoma). Compound 1 did not show an apparent activity in the same bioassays.     

Post-antibiotic effect of orbifloxacin against Escherichia coli and Pseudomonas aeruginosa isolates from dogs

Orbifloxacin is a fluoroquinolone drug used widely in companion animal medicine. In this study, we firstly determined post-antibiotic effects (PAEs) and post-antibiotic sub-minimum inhibitory concentrations (MIC) effects (PA-SMEs) of orbifloxacin for two strains each of Escherichia coli and Pseudomonas aeruginosa from dogs, and these parameters were compared with those of enrofloxacin. At twice the MIC, the PAEs of orbifloxacin ranged from -0.28-0.93 h (mean, 0.29 h) for E. coli and -0.18-1.18 h (mean, 0.37 h) for P. aeruginosa. These parameters were not significantly different for E. coli and shorter for P. aeruginosa, compared to enrofloxacin (P < 0.05). Continued exposure to 0.1, 0.2, and 0.3 the MIC of orbifloxacin resulted in average PA-SMEs of 0.55, 1.11, and 2.03 h, respectively, for E. coli, and 1.04, 1.40, and 2.47 h, respectively, for P. aeruginosa. These PA-SMEs, which had no significant differences with those of enrofloxacin, were significantly longer than the corresponding PAEs (P < 0.05). These results suggest that the PA-SME of orbifloxacin for E. coli and P. aeruginosa can be meaningfully prolonged by increase of sub-MICs.