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VL Grillo KE Arzey PM Hansbro AC Hurt S Warner J Bergfeld GW Burgess B Cookson CJ Dickason M Ferenczi T Hollingsworth MDA Hoque RB Jackson M Klaassen PD Kirkland NY Kung S Lisovski MA O'Dea K O'Riley D Roshier LF Skerratt JP Tracey X Wang R Woods L Post 《Australian veterinary journal》2015,93(11):387-393
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High susceptibility and low resistance allele frequency of Chrysodeixis includens (Lepidoptera: Noctuidae) field populations to Cry1Ac in Brazil 下载免费PDF全文
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Abstract Extract A single case of the inherited lysosomal storage disease known as mucopolysaccharidosis-IIIA (MPS-IIIA), due to a deficiency of the enzyme heparan sulphatase, was reported in an 18-month-old male Huntaway dog (Jolly et al 2000). He had developed normally but when presented with a history of progressive ataxia over the preceding month, had a high stepping, prancing gait and difficulty in jumping into a utility vehicle. In addition, he had started to defaecate in his kennel. Following diagnosis of the enzyme deficiency, the mutant heparan sulphatase gene was sequenced and a PCR/restriction enzyme diagnostic test developed, based on the mutation. This is capable of detecting both homozygous and heterozygous individuals (Yogalingam et al 2000). 相似文献
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Extract A lethal congenital polycystic kidney disease (PCKD) has been known in lambs in New Zealand for more than 50 years and assumed to be genetic in origin. We have studied this in lambs from an experimental mating in a flock experiencing an outbreak the previous year. Two Perendale rams identified by DNA profiling (SignaGen) as sires of PCKD lambs were each mated to the other's daughters. From 71 such matings, 10 ewes gave birth to 15 PCKD lambs of both sexes. The results are in accord with those expected for a recessive autosomal trait. All lambs were dead or died shortly after birth. Their abdomens were distended by enlarged kidneys 40–140 mm in length. Their cut surface showed a multitude of cystic spaces 1–5 mm in diameter in both the cortex and medulla. Livers of affected lambs showed complex tubular and cystic patterns of bile ducts containing bile, and supported by an excess of fibrous tissue. Cystic changes also occurred in the pancreas and salivary glands. 相似文献
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Extract We wish to record the source of Figures 1a and 2 from the report “Porcine dermatitis and nephropathy syndrome in New Zealand” published in the New Zealand Veterinary Journal 56, 94–99, 2008. These images were kindly provided by AF Julian from Gribbles Veterinary, Hamilton, New Zealand, and illustrate the gross cutaneous and renal lesions that were typical of those seen in pigs in the reported disease outbreak. The images were obtained from a pig with porcine dermatitis and nephropathy syndrome submitted by one of the authors (DEBL) from a similar outbreak of the disease that was examined by Gribbles Veterinary, Hamilton. The failure to properly acknowledge this contribution is regretted. 相似文献
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AC Becher J Spergser C Aurich E Zottler JE Aurich S Schäfer‐Somi 《Reproduction in domestic animals》2013,48(6):961-966
The aim of this study was to examine effects of an antibiotic combination at different concentrations on growth of mycoplasma and ureaplasma during cooled storage of canine semen (n = 20). Semen aliquots were diluted with Tris–citric acid–fructose–egg yolk extender containing either 1.0 g/l streptomycin and 0.6 g/l benzylpenicillin (control) or a combination of gentamycin, tylosin, lincomycin and spectinomycin (GTLS‐1: 0.25, 0.05, 0.15 and 0.3; GTLS‐2: 0.5, 0.1, 0.3 and 0.6; GTLS‐3: 1.0, 0.2, 0.6 and 1.2 g/l). Samples were assessed for motility and membrane integrity by computer‐assisted sperm analysis immediately after dilution and at 24, 48 and 72 h of cooled storage. Morphologically, normal spermatozoa were determined, and bacterial culture was performed at 24 and 72 h. Mycoplasma spp. were detected in 14 of 20 ejaculates (70%) with severe growth in 12 samples. A reduction but not total elimination of mycoplasma growth occurred in all GTLS extenders with the most pronounced reduction in group GTLS‐3 (control vs GTLS‐1 and GTLS‐2 p < 0.05, control vs GTLS‐3 p < 0.001). Ureaplasmas were detected in four ejaculates, and growth was reduced to the same extent in GTLS and control extender. Progressive motility in all groups, total motility in groups GTLS 1–3 and percentage of membrane‐intact spermatozoa in groups GTLS 2 and 3 decreased slightly (p < 0.05) over time. In conclusion, dilution of canine semen with GTLS extender has no major detrimental effects on spermatozoa during cooled storage. It reduced the growth but did not totally eliminate mycoplasmas and ureaplasmas from cooled‐stored dog semen. 相似文献
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Effect of Follicle Size on In Vitro Maturation of Pre‐Pubertal Porcine Cumulus Oocyte Complexes 下载免费PDF全文
Very small follicles (<3.0 mm diameter) are over‐represented on the surface of ovaries of non‐cycling pigs, and the oocytes collected from these follicles generally have reduced developmental competence in vitro. This study examined the effect of follicle size on the nuclear maturation (n = 608), the potential of parthenogenetic activation (n = 243) and the cyclic AMP (cAMP) content of pre‐pubertal porcine oocytes (n = 480). In addition, the influence of follicle size on steroid hormone synthesis was analysed. Cumulus oocyte complexes (COCs) flushed from small (2.5–4.0 mm) or large (4.5–6.0 mm) ovarian follicles were cultured for 0, 28 and 46 h. After 46 h of IVM, a greater proportion of oocytes from 4.5‐ to 6.0‐mm follicles reach metaphase II (MII) compared with those from follicles with 2.5–4.0 mm of diameter (96.1 vs 77.0%, respectively; p < 0.001). Parthenogenetic activation of oocytes from large follicles produced higher developmental rates than oocytes from large follicles (p < 0.05). At 28 h, the IVM medium with oocytes from large follicles contained significantly more 17ß‐oestradiol (E2) than the medium with oocytes from small follicles (5.55 vs 3.45 ng/ml, respectively; p < 0.05) and at 46 h, the medium with oocytes from small follicles contained significantly more progesterone (P4) than the medium with oocytes from large follicles (276.7 vs 108.2 ng/ml, respectively, p < 0.05). Porcine oocytes from large follicles have higher nuclear and cytoplasmic maturation capacities, but the differences did not appear to be cAMP‐mediated. Our findings also suggest that COCs from small follicles undergo more intensive luteinization than COCs from large follicles. The results show that oocytes from follicles with a diameter greater than 4.0 mm are more suitable for in vitro studies. 相似文献
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Reproductive Performance of Gilts with Similar Age but with Different Growth Rate at the Onset of Puberty Stimulation 总被引:1,自引:0,他引:1
R Kummer ML Bernardi AC Schenkel WS Amaral Filha I Wentz FP Bortolozzo 《Reproduction in domestic animals》2009,44(2):255-259
The aim of this study was to evaluate the reproductive performance of gilts that had a similar age but different weights at the onset of puberty stimulation by boar exposure at 144 days. Gilts were divided into two groups according to their lifetime growth rate from birth to approximately 144 days of age. Mean growth rates at this moment were 577 and 724 g/day for group 1 (G1; n = 58) and group 2 (G2; n = 58), respectively. After selection, gilts were weighed at approximately 155, 165 and 175 days of age, on the insemination day and at slaughter. Gilts were inseminated, on average, at 193 days of age and were slaughtered 32 days after insemination, when the number of corpora lutea and embryos were recorded. Higher growth rate gilts (G2) reached puberty earlier (155.3 vs 164.1 days; p < 0.01). More gilts of G2 group attained puberty by 190 days of age (p = 0.004) than G1 gilts (95%; 55/58 vs 76%; 44/58). The anoestrous rate, until 60 days after the onset of boar exposure was higher (p < 0.01) in G1 (19.0%; 11/58) than in G2 (3.4%; 2/58) group. However, there were no differences in the pregnancy rate (90.7 vs 94.5), ovulation rate (15.9 vs 16.5), total embryos (12.9 vs 11.7), viable embryos (12.0 vs 11.1) and embryo survival (73.7% vs 68.5%), between G1 gilts and G2 gilts, respectively (p > 0.05). High growth rate gilts attain puberty earlier and have a lower anoestrous rate than low growth rate gilts. 相似文献