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81.
Landscape Ecology - Human activities have led to the degradation of wetlands, impinging on their capacity to deliver essential ecosystem services to society. Wetland restoration now appears an...  相似文献   
82.
A unicameral bone cyst in the proximal humerus of a 3-year-old Norwegian forest cat was diagnosed by dynamic contrast-enhanced magnetic resonance imaging, surgical exploration, and histopathology. Surgical curettage and incorporation of bone cement led to full recovery. An osteosarcoma developed at the surgical site 17 months later. Thoracic radiographs showed pulmonary lesions consistent with metastasis.  相似文献   
83.
Bloodforms of Trypanosoma brucei brucei STIB 247 taken from rats and containing more than 80 per cent short stumpy forms, differentiated in vitro to procyclic forms in medium SDM 79 (Brun and Sch?nenberger 1979), enriched with 3 mmol.dm-3 cis-aconitate. Cell division was abolished by the addition of hydroxyurea (200 micrograms.ml-1) or arabinosyl adenine (20 micrograms.ml-1 to the cultivation medium, or by the omission of serum from the medium. The ultrastructure of exponentially growing controls was rearranged within 24 h. The endogenous respiration and the respiration stimulated by proline, succinate, and 2-oxoglutarate were detectable within 12 h; after 48 h the respiration rates were comparable to those found in the established procyclic forms. After 12 h the respiration was inhibited by 200 mumol.dm-3 KCN, and by 20 mumol.dm-3 antimycin to the extent found in procyclic forms. Hydroxyurea did not significantly affect respiration. Activities of procyclic-stage enzyme markers malate dehydrogenase, threonine dehydrogenase, succinate: cytochrome c reductase, and NADH: cytochrome c reductase rose within 48 h of differentiation to values which were close to those found in established procyclic forms. The activity of glutamate dehydrogenase (NAD-specific), however, was only 1/3 of that in the procyclics, and no citrate synthase was detected in differentiating culture. Glycosomal malate dehydrogenase was detected after 6 h. In the presence of hydroxyurea or arabinosyl adenine, or in the absence of serum, respiration rates, marker enzyme activities, and glycosomal malate dehydrogenase developed to the extent comparable to the untreated controls. The results suggest that it is possible to separate the process of differentiation from cell proliferation. Cell division is not a necessary prerequisite of differentiation.  相似文献   
84.
The ultrastructure of the flame cell, excretory capillaries, ducts, collecting ducts, excretory bladder, and excretory pore of Brachylaimus aequans was studied 6-8 days p.i. The excretory ducts, collecting ducts and excretory bladder are provided with numerous lamellae on the luminal side. The cilia of lateral flames in the excretory ducts have a triated rootlet. The excretory pore is covered by a tegument identical with the body tegument. The syncytium of the excretory bladder is connected with the tegument of the excretory pore by means of a septate desmosome. No lipid or excretory corpuscles have been demonstrated in the excretory system.  相似文献   
85.
We tested the virulence of 15 strains of Entamoeba histolytica, imported to Czechoslovakia, by intracaecal inoculation of laboratory rats. According to the scoring system of Neal, none of the 15 strains possessed the virulence index greater than 2. This indicates that all the organisms tested should be classified as avirulent. However, it should be noted that all the strains produced infection of the caecum and thus should be considered infective for rat. For 7 strains, isoenzyme patterns were determined for PGM, HK and ME. One imported strain, obtained from student from Congo, demonstrated isoenzyme pattern for PGM and HK indicated that the strain was virulent. This organisms had the index of virulence 1.8 (avirulent) in animal experiment; it was isolated from cysts of clinically asymptomatic patient. Examination of the rectal mucosa of the donor of the strain indicated typical chronic catarrhal proctitis of mild degree. Examination of the patient's serum demonstrated the presence of anti E. histolytica antibodies by CIEP, while the ELISA test was negative. Twenty-one cyst carriers were examined by rectoscopy. Pathologic changes were observed in 20 of these, as follows: altered vascular structure (13X), roughened mucosa (12X), mucosal reddening (10X), decreased glistening (7X), mucus in mucosa (5X), inflammatory pseudopolypes (2X), ulcers (2X), enanthema (1X). Histological biopsies were obtained in 15 cases. One was considered normal. Remaining 14 biopsies exhibited following morphological changes: increased mucus secretion (8X), edema (7X), lymphocytic and plasmocytic infiltration (6X), lymphocytic and plasmocytic infiltration in addition to the presence of eosinophilic granulocytes (6X), presence of mucophages (5X), haemorrhages (4X), increased vascularity (3X), lymphocytic and plasmocytic infiltration with presence of extremely abundant eosinophilic granulocytes (1X), erosive-ulcerative changes of mucosa (1X). The changes observed indicated chronic catarrhal proctitis with expression to greater or less degree of signs of chronic catarrhal inflammation.  相似文献   
86.
The process of colonization of Ascaris lumbricoides eggs by the fungus Verticillium chlamydosporium was studied by scanning electron microscopy. The preparations were made by fractionation of egg suspension exposed to the fungus for four days and frozen in liquid nitrogen according to St?rba and Milácek (1986). Ovicidal fungus forms an abundant ramifying mycelial network in the area between the eggs. However, egg-shells are penetrated only by some hyphae without any penetration organs produced (simple hyphal penetration). In a liquid medium, after penetration, hyphae inside the eggs rapidly grow among inner structures of egg-shells and on the surface of developing larvae. In the next phase, hyphae colonize the developing larva. The eggs attacked by this fungus remain morphologically unchanged for a long time except the sites of penetration. Verticillium chlamydosporium is a fungus with unique ovicidal properties. It colonizes the eggs of Ascaris lumbricoides at all stages of embryo development and also attacks larvae inside the eggs.  相似文献   
87.
The paper presents the results of faunistic, ecological and viral investigations concerning phlebotomine sandflies in an endemic focus of visceral leishmaniasis in Yugoslavia. These investigations were carried out in the period from 1969 to 1981.  相似文献   
88.
Two pairs of hitherto unknown multiciliate sensory endings have been demonstrated in free-swimming cercariae of Echinostoma revolutum using transmission and scanning electron microscopy. This type of receptor consists of a bundle of 36--40 short, conically projecting cilia ensheathed by a collar formed by a circular evagination of tegument in shape of a cup. The cilia projecting from the centre of a widened nerve fibre, filled with electron-lucid vesicles, possess a well developed basal body and do not contain a ciliary rootlet. The function of this receptor is discussed.  相似文献   
89.
A focal uterine adenomyosis is described in two bitches. In both cases, the uterus showed knobbly enlargements of 4 to 8 cm in diameter, which resulted in distinct clinical symptoms. Other pathological changes of the uterus were not present. One bitch was presented because of a history of vaginal discharge of several months' duration. Radiographs, as well as ultrasonography, revealed a soft tissue lesion at the cervix. The other bitch showed a marked reduction in its general condition and a sudden onset of a tense abdomen. Radiologically, a lesion of soft tissue opacity was observed in the mid-abdomen and was seen to originate from the left uterine horn during exploratory laparotomy. A torsion of the lesion was present, which explained the clinical signs in this second case.  相似文献   
90.
Sustainable animal production requires a healthy production system including healthy animals producing wholesome products. Epidemiology as a discipline is concerned with the occurrence of disease or disease indicators in populations as well as with factors influencing disease occurrence. By providing this information, epidemiology contributes significantly to the development and maintenance of healthy livestock. This is illustrated with the example of the enzootic pneumonia (EP) eradication programme of Switzerland. A series of observational studies were conducted in order to obtain the necessary knowledge to implement an area-wide EP eradication programme. The issues of economical eradication strategies at the herd level and the risk of re-infections were addressed. Simulation modelling and economical analysis were also applied. These research projects led to the successful start of a national eradication programme. The example of the EP eradication programme illustrates that applied epidemiological research provides critical information for the planning, implementation and evaluation of disease control programmes and is thus a key tool in developing sustainable animal production.  相似文献   
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