Genotype,ultraviolet irradiation,and harvesting time interaction effects on secondary metabolites of whole lettuce and browning of fresh-cut product

This study evaluated the importance of three factors (genotype, ultraviolet [UV] radiation and harvest time) and their interaction on phenolics, tannins, flavonoids and antioxidant activity of ‘Paris Island’ (romaine type) and ‘Sanguine’ (red leaf type) lettuce plants as well as on the severity of browning of their fresh-cut product. Ten genotypes had significant effect on secondary metabolite accumulation and higher levels were found in ‘Sanguine’, while no interaction effect was found among all the studied factors. Therefore, a new analysis of variance was performed for each genotype separately. The results showed that in ‘Paris Island’ the time of harvest had a significant effect on all measured parameters with higher levels for plants from the night harvest, while UV radiation had little effect on the plants’ biochemistry. On the contrary, in ‘Sanguine’, UV radiation significantly affected the concentration of secondary metabolites and antioxidant capacity, with higher levels for plants grown under UV-open film. Browning evaluation of fresh-cut product derived from the above plants showed that factors affecting secondary metabolite concentration in whole plants at harvest did not have a similar effect. The findings indicate that there are appropriate treatments, for each genotype, that could achieve high production of secondary metabolites and improve their antioxidant properties.     

Epidemiological basis useful for the control of foot-and-mouth disease

Although known for many years, foot-and-mouth disease is still able to represent a real threat to many farming economies in the world. The recent 2001 Western European epizootics linked to O PanAsia virus strain can illustrate the fact that many questions are still unanswered in the field of foot-and-mouth epidemiology. It also demonstrates that the increase in international trade, including livestock, animal products and animal food, means an increase in the probability of transmitting, through the same way, some animal diseases, foot-and-mouth included. In our economies, a rapid identification of the virus and a fast elimination of infected, contaminated and even some contact animals are still the key factors to react in front of such a disease.

Résumé

Bien que connue depuis des années, la fièvre aphteuse représente toujours une menace réelle pour beaucoup d'économies agricoles de la planète. L'épisode récent de 2001 en Europe Occidentale, lié au virus O PanAsia, illustre le fait que de nombreuses questions sont toujours sans réponse au niveau de l'épidémiologie de la maladie. Cet épisode démontre aussi que le développement du commerce international des animaux et des produits animaux se traduit par une augmentation de la probabilité de transmettre, de la même façon, diverses maladies animales dont la fièvre aphteuse. Dans nos économies, une identification rapide du virus, suivie d'une élimination précoce des animaux malades, contaminés, voire contact, restent les clefs d'une maîtrise de la fièvre aphteuse.     

Genetic diversity and thermotolerance in Holstein cows: Pathway analysis and marker development using whole-genome sequencing

Heat stress causes extensive losses in the dairy sector, due to negative effects on milk production and reproduction. Cows have evolved a series of protective mechanisms, (physiological, biochemical, behavioural) to cope with the thermostressing environments, which have allowed the preservation of productive and reproductive potential of specific animals during summer; these animals are considered thermotolerant and could be used to design programs of selective breeding. These programs, targeting the generations of a population of heat-resistant animals, would increase the frequency of the desired phenotypes, tackling the financial losses on one hand and reducing the carbon footprints of the dairy sector on the other. The development of genomics techniques has enabled genome wide variant calling, to detect SNPs associated with the desired phenotypes. In this study, we used a comparative genomics approach to detect genetic variation associated with thermotolerance and to design molecular markers for characterizing the animals as tolerant/sensitive. A total of 40 cows from each group were split in four sequencing pools and a whole-genome sequencing approach was used. Results and conclusion: Genome-wide genetic variation between groups was characterized and enrichment analysis revealed specific pathways which participate in the adaptive mechanisms of thermotolerance, implicated into systemic and cellular responses, including the immune system functionality, Heat Stress and Unfolded Protein Response. The markers made a promising set of results, as specific SNPs in five genes encoding for Heat Shock Proteins were significantly associated with thermotolerance.