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Microbial community composition and carbon cycling within soil microenvironments of conventional, low-input, and organic cropping systems 总被引:1,自引:0,他引:1
This study coupled stable isotope probing with phospholipid fatty acid analysis (13C-PLFA) to describe the role of microbial community composition in the short-term processing (i.e., C incorporation into microbial biomass and/or deposition or respiration of C) of root- versus residue-C and, ultimately, in long-term C sequestration in conventional (annual synthetic fertilizer applications), low-input (synthetic fertilizer and cover crop applied in alternating years), and organic (annual composted manure and cover crop additions) maize-tomato (Zea mays - Lycopersicum esculentum) cropping systems. During the maize growing season, we traced 13C-labeled hairy vetch (Vicia dasycarpa) roots and residues into PLFAs extracted from soil microaggregates (53-250 μm) and silt-and-clay (<53 μm) particles. Total PLFA biomass was greatest in the organic (41.4 nmol g−1 soil) and similar between the conventional and low-input systems (31.0 and 30.1 nmol g−1 soil, respectively), with Gram-positive bacterial PLFA dominating the microbial communities in all systems. Although total PLFA-C derived from roots was over four times greater than from residues, relative distributions (mol%) of root- and residue-derived C into the microbial communities were not different among the three cropping systems. Additionally, neither the PLFA profiles nor the amount of root- and residue-C incorporation into the PLFAs of the microaggregates were consistently different when compared with the silt-and-clay particles. More fungal PLFA-C was measured, however, in microaggregates compared with silt-and-clay. The lack of differences between the mol% within the microbial communities of the cropping systems and between the PLFA-C in the microaggregates and the silt-and-clay may have been due to (i) insufficient differences in quality between roots and residues and/or (ii) the high N availability in these N-fertilized cropping systems that augmented the abilities of the microbial communities to process a wide range of substrate qualities. The main implications of this study are that (i) the greater short-term microbial processing of root- than residue-C can be a mechanistic explanation for the higher relative retention of root- over residue-C, but microbial community composition did not influence long-term C sequestration trends in the three cropping systems and (ii) in spite of the similarity between the microbial community profiles of the microaggregates and the silt-and-clay, more C was processed in the microaggregates by fungi, suggesting that the microaggregate is a relatively unique microenvironment for fungal activity. 相似文献
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Two complementary studies were performed to examine (1) the effect of 18 years of nitrogen (N) fertilization, and (2) the effects of N fertilization during one growing season on soil microbial community composition and soil resource availability in a grassland ecosystem. N was added at three different rates: 0, 5.44, and 27.2 g N m−2 y−1. In both studies, Schizachyrium scoparium was the dominant plant species before N treatments were applied. Soil microbial communities from each experiment were characterized using fatty acid methyl ester (FAME) analysis. Discriminant analysis of the FAMEs separated the three N fertilizer treatments in both experiments, indicating shifts in the composition of the microbial communities. In general, plots that received N fertilizer at low or high application rates for 18 years showed increased proportions of bacterial FAMEs and decreased fungal FAMEs. In particular, control plots contained a significantly higher proportion of fungal FAMEs C18:1(cis9) and C18:2(cis9,12) and of the arbuscular mycorrhizal fungal (AMF) FAME, C16:1(cis11), than both of the N addition treatment plots. A significant negative effect of N fertilization on the AMF FAME, C16:1(cis11), was measured in the short-term experiment. Our results indicate that high rates of anthropogenic N deposition can lead to significant changes in the composition of soil microbial communities over short periods and can even disrupt the relationship between AMF and plants. 相似文献
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Microbial activity is known to continue during the winter months in cold alpine and Arctic soils often resulting in high microbial biomass. Complex soil nutrient dynamics characterize the transition when soil temperatures approach and exceed 0 °C in spring. At the time of this transition in alphine soils microbial biomass declines dramatically together with soil pools of available nutrients. This pattern of change characterizes alpine soils at the winter-spring transition but whether a similar pattern occurs in Arctic soils, which are colder, is unclear. In this study amounts of microbial biomass and the availability of carbon (C), nitrogen (N) and phosphorus (P) for microbial and plant growth in wet peaty soils of an Arctic sedge meadow have been determined across the winter-spring boundary. The objective was to determine the likely causes of the decline in microbial biomass in relation to temperature change and nutrient availability. The pattern of soil temperature at depths of 5-15 cm can be divided into three phases: below −10 °C in late winter, from −7 to 0 °C for 7 weeks during a period of freeze-thaw cycles and above 0 °C in early spring. Peak microbial biomass and nutrient availability occurred early in the freeze-thaw phase. Subsequently, a steady decrease in inorganic N occurred, so that when soil temperatures rose above 0 °C, pools of inorganic nutrients in soils were very low. In contrast, amounts of microbial C and soluble organic C and N remained high until the end of the period of freeze-thaw cycles, when a sudden collapse occurred in soluble organic C and N and in phosphatase activity, followed by a crash in microbial biomass just prior to soil temperatures rising consistently above 0 °C. Following this, there was no large pulse of available nutrients, implying that competition for nutrients from roots results in the collapse of the microbial pool. 相似文献
57.
Fernando Machado-Stredel Benedictus Freeman Daniel Jiménez-Garcia Marlon E.Cobos Claudia Nu?ez-Penichet Laura Jiménez Ed Komp Utku Perktas Ali Khalighifar Kate Ingenloff Walter Tapondjou Thilina de Silva Sumudu Fernando Luis Osorio-Olvera A.Townsend Peterson 《中国鸟类》2022,(1):1-10
Studies of biodiversity dynamics have been cast on either long(systematics)or short(ecology)time scales,leaving a gap in coverage for moderate time scales of de... 相似文献
58.
Keld Sorensen PhD Dean P. Neely VMD PhD William Read Paul M. Grappell MD 《Journal of Equine Veterinary Science》1990,10(6):417-421
An assay for the determination of the concentration of an equine fetal protein (EQFP) in pregnant mares serum was developed and used to quantitate more than 2000 serum samples from Thoroughbred mares during pregnancy. The concentration of EQFP increased throughout pregnancy. Mares with unproblematic (normal) pregnancies were used to establish the reference values at given gestational ages (GA). Mares with pregnancy failures (n=58) were found to have EQFP concentrations outside the reference values established at given GA's; 10 were high and 48 were low. Twin pregnancies (n=21), placentitis and impending abortion (n=71) were associated with elevated concentration. Twin pregnancies were detected by elevated EQFP concentration, in one case as early as 12 days after breeding. 相似文献
59.
A modified lateral spinal decompression technique was performed in 61 dogs with thoracolumbar disc protrusion. Myelography combined with plain radiography and neurological examination determined the side of greatest compression in 93 per cent of the dogs. Disc material was retrieved in 98 per cent of the cases. Of the 35 non-ambulatory dogs, 95 per cent regained the ability to walk. The recovery time was three weeks. 相似文献
60.
S M Stover B J Johnson B M Daft D H Read M Anderson B C Barr H Kinde J Moore J Stoltz A A Ardans 《Equine veterinary journal》1992,24(4):260-263
Twenty-one horses had a complete unilateral humeral fracture during race training or racing at a California racetrack during the period 24 February 1990 to 10 July 1991. Fractures occurred approximately equally in left and right limbs, and in males and females. Most fractures occurred during training, and in 2- and 3-year-old horses. Only 5 of 16 Thoroughbred horses with known racing records had previously raced more than once, and their mean time between races was less than the time between their last race and fracture (P = 0.07). Ten of 13 humeri studied further had gross evidence of periosteal callus bridging one portion of the fracture line, indicative of a pre-existing stress fracture. 相似文献