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Several methods were used in an attempt to develop an age and growth model for the Atlantic angel shark (Squatina dumeril). Band counts from vertebral sections, which were fit to the traditional von Bertalanffy growth equation, the Gompertz growth equation, and the two-parameter von Bertalanffy growth equation, did not produce realistic parameter estimates. Additionally, a length-based Bayesian model was applied to fishery-independent length–frequency data, and a full Bayesian model was fitted to length-at-age data to estimate parameters for von Bertalanffy growth equation. Both the length-based and full Bayesian models failed to converge; the length–frequency data showed high bimodality unrelated to season, year, or other factors, and band counts were not predictable by length. Vertebral band counts were not valid for ageing Atlantic angel sharks, and length-based methods, which require normally distributed length–frequencies, were not appropriate for this data set. This study represents the first attempt at modeling age and growth for this species and provides research guidelines for future research initiatives.  相似文献   
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Anticoagulant rodenticides (ARs) are considered inhumane, show increasingly limited efficacy due to acquired resistance, and carry environmental consequences associated with non-target species uptake. In a questionnaire study of 499 UK farms that all deployed chemical rodenticide we found a high mean reliance (79%), on second generation ARs with just over half of the respondents using no other rodent control methods. Additional methods where deployed, alone or in combination, included predation (41%), kill-trap deployment (16%) and shooting (1%). Nearly 40% of all respondents deployed rodenticides year-round. There was no evidence to suggest that “tidy-farm” measures, such as clearing food spills and minimising on-farm rodent harbourage sites aimed at minimising rodent-associated problems, were associated with a lower likelihood of year-round deployment; in fact trends in our analyses suggested the opposite. We therefore encourage operators to fully evaluate the true necessity of rodenticide deployment before AR use.  相似文献   
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We analyzed the spreading and persistence of PSTVd variants in several ornamentals in the territory of the Czech Republic. The pool of PSTVd variants detected in Solanum jasminoides, S. muricatum, Datura sp. and Brugmansia sp. was biolistically transferred to Matricaria chamomilla, Argyranthemum frutescens and Diascia sp., species which we found as sensitive hosts for PSTVd from ornamentals. The PSTVd pool showed sequence changes and increased variation after its transfer to potato, suggesting a wide adaptation potential of PSTVd in this crop. Potato exhibited genotype-dependent leaf and spindle tuber symptoms, when inoculated with the sap from S. jasminoides infected with the predominant and sequence-stable PSTVd-S1.  相似文献   
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The wedge-tailed eagle is Australia’s largest bird of prey and one of the largest eagles in the world. Aquila audax fleayi is an endemic Tasmanian subspecies isolated for 10,000 years from the nominate subspecies on the Australian mainland. The Tasmanian wedge-tailed eagle is classified nationally and at a State level as endangered due to its small number of breeding pairs, low breeding success and high rate of mortality from unnatural causes. The subspecies experiences mortality throughout its range from shooting, poisoning, trapping, road accidents, electrocutions and collisions with wind turbines, aircraft, fences and overhead wires, which we term ‘un-natural mortality’. A portion of the subspecies’ range is managed for timber production, which can lead to disturbance of nest sites and the loss of nest trees. We use a model of the eagle population from the Bass District in northeast Tasmania to explore the relative importance of different sources of mortality and nesting habitat loss, and the potential for mitigating impacts associated with unnatural mortality, disturbance, nesting habitat loss and human access to forests. We create a habitat map including suitable nest sites and link it to a dynamic landscape population model based on life history traits and disturbance responses. Using the program RAMAS-Landscape, we model alternative forest management scenarios, ranging from no timber harvesting and a natural wildfire regime, to scenarios prescribing native forest harvesting and regeneration and different levels of conversion of native forest to plantation under the same natural wildfire regime. The results indicate that the Tasmanian wedge-tailed eagle is sensitive to unnatural mortality, plantation establishment and native forest harvesting. The predicted decline over the next 160 years (65%) will most likely be driven largely by loss of current and potential future nest sites associated with harvesting activities, exacerbated by unnatural mortality in the wider landscape. Interventions that minimise unnatural mortality, reduce nest disturbance, and retain breeding habitat and nest sites may improve the prospects for the subspecies in the Bass District. If nest disturbance and unnatural mortality continue at the rates modelled here, the species appears to face a high risk of declining substantially in the region.  相似文献   
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Soil physicochemical properties and microbial communities are highly heterogeneous and vary widely over spatial scales, necessitating careful consideration of sampling strategies to provide representative and reproducible soil samples across field sites. To achieve this, the study aimed to establish appropriate sampling methodology and to determine links between the variability of parameters, utilising two sampling strategies. The first (design 1) involved extracting 25 cores from random locations throughout the field and pooling them into five sets of five cores. The second (design 2) involved a further 25 cores within five 1 m2 sub-plots. Sub-samples from each sub-plot were pooled in order to determine between and within sub-plot variability. All samples were analysed independently and as pooled sub-samples. Results indicate that pooling spatially separated samples significantly reduced the variability in pH, compared to individual samples. Pooling samples from a small area resulted in lower within sub-plot variability than between sub-plots for pH and bacterial community composition assessed by terminal-restriction fragment length polymorphism analysis. Following multivariate statistical analysis, a large amount of variation in community composition was explained by soil pH, which is remarkable given the relatively small size of the sampling area and minor differences in pH. Moisture content was also important in determining bacterial communities in the random design (design 1). In the 1 m2 sub-plot design (design 2), the spatial location of the plots explained a large degree of the variation in bacterial community composition between plots, which was due to spatial autocorrelation of pH and possible additional environmental parameters. This study emphasises the importance of sampling design for obtaining representative samples from soil.  相似文献   
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Overseeded winter annuals in bermudagrass [Cynodon dactylon (L.) Pers.] improve annual dry‐matter (DM) yield and capture nutrients in fields receiving manure application. This study determined the DM and nutrient uptake responses of annual ryegrass (Lolium multiflorum L.), cereal rye (Secale cereale), berseem clover (Trifolium alexandrinum L.) and bermudagrass‐winter fallow to 0, 50, 100 and 150 kg N ha?1 applied approximately 2 months before a single spring harvest, and in addition to swine‐effluent N (258 and 533 kg ha?1 in summer 2000 and 2001, respectively). Under drought conditions in 2000, DM yield at the spring harvest was highest in ryegrass, and summer DM yield of bermudagrass was greater at 100 and 150 kg N ha?1 than 50 kg N ha?1(P < 0·05). The concentration and uptake of N at the spring harvest increased linearly across N rates in both years (P < 0.05). Cover crops differed in N uptake in 2000 (P < 0.01) and values ranged from approximately 141 kg N ha?1 in berseem clover to 86 kg N ha?1 in rye. Per unit of N applied, uptake of N increased by approximately 0·409 kg ha?1 in 2000 and 0·267 kg ha?1 in 2001; uptake of P increased by 0·029 and 0·014 kg ha?1 respectively. In 2000, uptake of P was responsive to N rate and this relationship was significant (P < 0·01) in winter fallow (slope = 0·032) and ryegrass (slope = 0·057). Increased uptake of N and P at the single spring harvest was due mainly to higher concentrations in herbage and not higher DM yield.  相似文献   
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