抗犬细小病毒的单克隆抗体的制备

CPV YZ株的细胞培养液经蔗糖密度梯度离心纯化后作为免疫原免疫BALB/C小鼠 ,应用杂交瘤技术筛选出 8株能稳定分泌单克隆抗体的杂交瘤细胞 ,分别命名为F5G10、3F11F4、F11G6、6C5C2、6E4G3、6C2B11、F11D6、3D9C4株 ,各株单抗均为IgG型 ;中和试验表明 :F11G6、3D9C4、6E4G3、6C5B114株单抗有中和作用 ,Dot ELISA试验证明了 8株单抗仅针对病毒抗原决定簇的空间结构。     

不同降雨强度下滨海盐渍土水盐运动规律模拟实验研究

采用原状土柱 ,对滨海盐渍土的水盐运动进行了室内模拟研究。结果表明 :7,14和 2 8mm的雨量下 ,滨海盐渍土土壤剖面的水分运动规律基本相似 ,水分运动的整个过程可分为两个阶段 ,即入渗控制阶段和蒸发控制阶段。第 1阶段经历的时间很短 ,第 2阶段经历的时间较长 ,其水吸力随时间变化趋势符合对数方程 ,达到极显著水平。剖面上盐分运动规律却具有明显的差异性 :小雨时土体中的盐分再分布十分缓慢 ,甚至基本没有变化 ;中雨和大雨时土体中的盐分明显发生再分布 ,且也可以分成两个阶段 ,即盐分迅速下降阶段和盐分缓慢上升阶段。中雨和大雨第 2阶段盐分随时间的变化趋势也符合对数方程 ,达到极显著水平。就脱盐效果而言 ,小雨很难使土体脱盐 ;中雨能使土体在短时间内部分脱盐 ,但长期而言 ,整个剖面脱盐效果不理想 ;大雨能使整个土壤剖面长期处于脱盐状况 ,因此脱盐效果好     

A comparison of popular approaches to optimize landscape resistance surfaces

Landscape Ecology - Landscape resistance surfaces are often used to address questions related to movement, dispersal, or population connectivity. However, modeling landscape resistance is...     

Radiation-Induced Breeding in Camelina Sativa

Journal of Crop Science and Biotechnology - The ionizing radiation methods, including X-rays, gamma rays, and neutrons to create variation is well established and used in major crops. Many...     

紫花苜蓿耗水规律及灌溉制度优化研究

为研究河套灌区紫花苜蓿的耗水规律,同时对现有灌溉制度进行优化,在田间试验的基础上,设置不同灌水水平对前两茬苜蓿进行了研究。结果表明:现蕾期和开花期是紫花苜蓿耗水的旺盛阶段,该阶段土壤水分亏缺会直接影响苜蓿产量的形成。通过多元线性回归的方法求解得到了两茬紫花苜蓿各生育期的敏感性系数,分别在0.103~0.731和0.041~0.375之间,且以现蕾期和开花期为最大,说明这两个生育阶段为苜蓿生长的关键阶段。以Jensen模型为基础,采用最小二乘法初步优化了两茬紫花苜蓿生育期内的灌溉制度,第1茬灌溉定额以1 400 m3/hm~2为宜,第2茬以2 300 m3/hm~2为宜,两茬灌水次数分别以4次和5次为最优。     

Prediction of parental combination for introduction of stay-green associated loci in wheat

In wheat, the increase of yield and stability associated traits can be achieved by combining parents containing the stay-green trait and favorable alleles for grain yield. The aim of this work was to analyze the genetic dissimilarity between wheat lines from stay-green and synchronized maturation groups and elite cultivars. Moreover, to propose promising combinations seeking the selection of high-grain yield and high bread-making quality genotypes containing stay-green trait. The experiment was conducted in a randomized block design with three replications in 2003, 2004, and 2005, using sister-lines with the presence and absence of stay-green trait and elite cultivars. Genetic variability exists among wheat strains from the synchronized stay-green maturation group and elite cultivars. Genotypes of maturation group stay-green obtained an average performance superior to the synchronized group. Crosses between stay-green lines and the CEP 29 and BRS 177 cultivars are promising in the selection of genotypes carrying the stay-green trait with high yield and bread-making quality.