Ocular tissue changes associated with anterior segment opacity in lumpfish (Cyclopterus lumpus L) eye

Lumpfish use their vision to hunt prey or, in the case of aquaculture, to see and eat pelleted diets. A common anterior ocular opacity abnormality designated in the literature as “cataract” described in both farmed and wild lumpfish has not yet been characterized in detail at the pathobiological level. We describe here lens tissue changes associated with cataract in cultured and domesticated lumpfish. Methodology included gross observations, ophthalmoscopy and histology. Young adult cultured animals approaching 400 days post‐hatch presented a range of anterior segment opacities associated with lenticular abnormalities observable at a histological level. Wild aged domesticated animals also displayed cataracts characterized mainly by abnormalities of the lens observed by both ophthalmoscopy and histology. Dysplastic lesions of the lens in one aged domesticated lumpfish were accompanied with both retinal and optic nerve degeneration. These novel naturally occurring anatomical changes in lumpfish present both commonalities and unique features associated with cataract in young adult cultured animals versus aged domesticated broodstock animals.     

167 individuals versus millions of hooks: bycatch mitigation in longline fisheries underlies conservation of Amsterdam albatrosses

1. Industrial fisheries represent one of the most serious threats worldwide to seabird conservation. Death of birds in fishing operations (i.e. bycatch) has especially adverse effects on populations of albatrosses, which have extremely low fecundity. 2. The single population worldwide of Amsterdam albatross (Diomedea amsterdamensis) comprises only 167 individuals and risks considerable decline over the mid‐term from additional mortality levels potentially induced by fisheries. The priority actions listed in the current conservation plan for this species included characterizing the longline fisheries operating within its range, dynamically analysing the overlap between albatrosses and these fisheries, and providing fisheries management authorities with potential impact estimates of longline fisheries on the Amsterdam albatross. 3. During all life‐cycle stages and year quarters the birds overlapped extensively with fishing effort in the southern Indian and Atlantic oceans. Fishing effort, and consequently overlap score (calculated as the product of fishing effort and time spent by the birds in a spatial unit) was highest in July–September (45% of the hooks annually deployed). Just three fleets (Taiwanese, Japanese and Spanish) contributed to >98% of the overlap scores for each stage (72% from the Taiwanese fleet alone, on average). Daily overlap scores were higher for the non‐breeding versus the breeding stages (3‐fold factor on average). 4. Based on previous bycatch rates for other albatross species, this study estimated that longline fisheries currently have the potential to remove ~2–16 individuals (i.e. ~5%) each year from the total Amsterdam albatross population, depending on whether bycatch mitigation measures were or were not systematically employed during the fishing operations. 5. Recent bycatch mitigation measures may be instrumental in the conservation of the Amsterdam albatross. This study suggests three further key recommendations: (1) to focus conservation efforts on the austral winter; (2) to require all operating vessels to report ring recoveries; and (3) to allocate special regulation of fishing operations in the areas of peak bycatch risk for the Amsterdam albatrosses. Copyright © 2015 John Wiley & Sons, Ltd.     

Fish community response to environmental variations in an impacted Neotropical basin

Fish communities’ organisation is a challenging task due to global, local and temporal variations related to biotic and abiotic factors, highlighting anthropic activities. The Verde River Basin (VRB) was chosen as a model to the fish community study due to its complexity, presenting a longitudinal gradient of degradation. The ichthyofauna and abiotic factors were sampled during twelve months in four sites. Analyses employed rarefaction curves with Hill numbers estimators and canonical correspondence analysis. The endemic Apareiodon sp. (not described), Hypostomus albopunctatus, H. strigaticeps, Oligosarcus paranensis, Neoplecostomus yapo and Trichomycterus diabolus were associated with structurally complex and well‐preserved environments. In contrary, Astyanax aff. fasciatus, Corydoras ehrhardti, Geophagus brasiliensis, H. ancistroides and Phalloceros harpagos showed tolerance to impacted environments. The methods here employed allowed to identify and explain differences in the ichthyofauna structure, distinguishing the influence of spatial, temporal and human effects on the fish communities.     

Application of multielement stable isotope ratio analysis to the characterization of French, italian, and spanish cheeses

The stable isotope ratios (delta13C, delta15N, and delta34S of casein and delta13C and delta18O of glycerol) measured by IRMS of French, Italian, and Spanish cheeses are presented and discussed. Variability factors such as animal-feeding regimen, geographical origin, and climatic and seasonal conditions were studied to check the possibilities of cheese characterization offered by each isotopic parameter. Delta13C values of both casein and glycerol appeared to be strongly correlated to the amount of maize in the animal diet. Delta15N and delta34S of casein proved to be mostly influenced by the geoclimatic conditions of the area (aridity, closeness to the sea, altitude). Delta18O of glycerol was more dependent on the geographical origin of the cheeses and on climatic/seasonal parameters. By applying a multivariate stepwise canonical discriminant analysis, good discrimination possibilities for the different European cheeses were obtained, confirmed by the classification analysis, when >90% of the samples were correctly reclassified.     

Bipolar membrane electroacidification of demineralized skim milk.

The aim of this study was to evaluate the effect of decreasing the mineral content of skim milk by electrodialysis (ED) prior to electroacidification with bipolar membrane (BMEA) on the performance of the process, the chemical composition, and the physicochemical and functional properties of the isolates produced. ED used to demineralize the skim milk solution was very efficient. However, the electroacidification parameters were influenced by the demineralization level of the skim milk solution: the energy efficiency was decreased with an increase in demineralization, but it was still possible to perform BMEA at a very low conductivity level. Moreover, the isolates produced by BMEA after electrodialysis demineralization at different rates showed similar chemical composition, except on potassium and lactose contents for 75% demineralized isolate. These isolates, except on protein load for 75% demineralization rate, showed similar physicochemical and functional properties, whatever the demineralization rate.     

Metabolic status of lactating cows given a hepatoprotectant and different feed energy sources

Tropical Animal Health and Production - Communal rearing has been reported in several mammals, including wild ungulates. However, until now, there was no evidence of any alloparental care in the...     

Fungal, bacterial and plant dsDNA contributions to soil total DNA extracted from silty soils under different farming practices: Relationships with chloroform-labile carbon

Twelve differently-managed silty soils from North-Western France were chosen to compare two common methods of quantifying soil microbial biomass: Chloroform fumigation and extraction-labile carbon (CL_C) and microbial double stranded DNA (dsDNA). We also determined the contributions of each of the fungal, bacterial, and plant kingdoms to the total community dsDNA using real-time Polymerase Chain Reaction with kingdom-specific ribosomal primer sets. Regardless of the method, the highest microbial biomasses were associated with long-term untilled plots. Site (locations) specificities could also be detected, especially in conventionally cultivated lands. Regardless of site, a strong linear relationship could be drawn between CL_C and dsDNA in tilled lands (r = 0.91, n = 15, P = 0.01) and in grasslands (r = 0.78, n = 21, P = 0.01). Moreover, we propose a logarithmic model describing all of our silty soils, irrespective of management. In order to explain the non-linearity (log) of this relationship, we tested the hypothesis of a weak plant dsDNA contribution in total dsDNA in comparison with the well-documented root cell contribution to CL_C quantifications. Plant dsDNA never exceeded 2.6% of total dsDNA content for all of the soils studied. Among groups examined, the bacterial dsDNA contribution to the community dsDNA pool was the most site- and/or pedoclimatic-dependent. Fungi constituted a major component of total microbial biomass in grassland or in land with permanent plant cover where their proportion reached almost 50% of total dsDNA. More precisely, fungal dsDNA concentration was highly related to tillage. Our study demonstrated the expediency of the total microbial dsDNA quantification in agricultural silty soils rather than the time-consuming quantification of CL_C. Quantifying the relative contribution of bacterial or fungal biomass in total dsDNA by real-time PCR allows to access to a new level of knowledge of the soil microbial biomass and to reveal the balances between those two kingdoms according to soils or farming practices.     

Humus macro-morphology and soil microbial community changes along a 130-yr-old Fagus sylvatica chronosequence

We aimed to characterize humus macro-morphology and the associated soil microbial community within the unmodified litter (OL), the fragmented and humified layers (FH) and the organo-mineral (A) layer along a beech (Fagus sylvatica L.) forest chronosequence with four stand age-classes (15-, 65-, 95-, 130-yr-old) in Normandy, France. Humus macro-morphology was described with 36 quantitative and semi-quantitative variables. We measured microbial biomass N (N_mic), microbial N quotient (N_mic-to-N_t), fungal ergosterol, bacterial and fungal DNA using 16S and 18S rDNA real-time qPCR and evaluated the potential metabolic profile of heterotrophic bacteria within each soil layer and stand age-class. The log-transform ergosterol/fungal DNA ratio (EFR index) was used as an indicator related to active fungal biomass and the fungal/bacterial (F/B) ratio was calculated from qPCR results. There was a shift from mull (mainly dysmull) to moder humus forms along the chronosequence. While the N_mic did not change significantly, the N_mic-to-N_t decreased along the chronosequence in the OL layer. Ergosterol content increased in FH and A layers and the F/B ratio increased in the FH layer with increasing beech forest age. The EFR index was significantly higher in the OL and A layers of the oldest stands, whereas the highest EFR index in the FH layer occurred in the 15-yr-old stands. The functional diversity of heterotrophic bacteria was greater within OL and FH layers of 130-yr-old stands, but highest in the A layer of 15-yr-old stands while the Average Well Color Development remained stable for all soil layers. We found significant correlations between macro-morphology and microbial variables, especially between FH-based morphology and fungal biomass. Our main results are that beech forest maturation is accompanied by (1) an increase in fungal biomass in the FH layers and, (2) an increase in heterotrophic bacteria functional diversity in the organic layers. We have identified key macro-morphology variables that are good predictors of the structural and functional profile of the soil microbial community during beech forest development.