POST-OPERATIVE COMPUTED TOMOGRAPHY IN TWO DOGS WITH CEREBRAL MENINGIOMA

Post-operative computed tomography (CT) has been described as a technique for diagnosing incomplete resection or recurrence of cerebral neoplasms in humans. The characteristics of immediate postoperative CT images in dogs with intracranial pathology are unknown. This report describes findings from preoperative, immediate post-operative, and 4 week to 9 month follow-up CT examinations in two dogs with histologically-confirmed cerebral meningiomas. In images of one dog after surgery there was mild contrast enhancement of the tissue surrounding the surgical site. This enhancement had resolved in later images and was probably the result of surgically induced trauma. In post operative images of the other dog there was significant hyperattenuation of the tissues around the surgical site. In post contrast images there was increased enhancement that was evident in later images. These findings, although not supported by necropsy, probably indicate incomplete excision of the tumor.     

COMPUTED TOMOGRAPHIC IMAGING OF THE EQUINE HEAD

The head from three horses euthanized due to diseases unrelated to the head and neck was imaged using computed tomography (CT). Gross cross-sectional slices of equine head #1 and skeleton of equine head #2 were compared with the CT images of the three equine heads to identify normal structures of the cranium, brain, paranasal sinuses, nasal cavity, and teeth. Labeled transverse CT images of the equine head are presented sequentially as a reference for normal anatomy.     

The effects of dietary n-3 vs. n-6 fatty acids on ex-vivo LTB4 generation by canine neutrophils

Dietary n-3 polyunsaturated fatty acids are widely used for amelioration of inflammatory skin disease in dogs. In this study, a diet containing two different sources of n-3 polyunsaturated fatty acid–triglyceride (from menhaden oil) and concentrated ethyl esters–was fed to one group of six purpose-bred dogs, while an isocaloric isonitrogenous diet with corn oil (n-6 polyunsaturated fatty acids) was fed to another group of eight purpose-bred dogs for six weeks. Peripheral blood neutrophils, isolated at week–1 (baseline), week 2 and week 6, were stimulated with calcium ionophore A23187 and the amount of leukotriene B₄ produced was determined via reversed-phase high performance liquid chromatography. Analysis of variance of log-transformed data revealed a significant effect for diet ( P  = 0.005) at six weeks, with dogs fed the high n-3 polyunsaturated fatty acid diet having significantly less mean ex vivo neutrophil leukotriene B₄ production than dogs fed the high n-6 polyunsaturated fatty acid diet. Further studies on the clinical usefulness of n-3 polyunsaturated fatty acid ethyl esters are warranted.     

A rabbit model of non-typhoidal Salmonella bacteremia

Bacteremia is an important cause of morbidity and mortality in humans. In this study, we focused on the development of an animal model of bacteremia induced by non-typhoidal Salmonella. New Zealand White rabbits were inoculated with a human isolate of non-typhoidal Salmonella strain CVD J73 via the intra-peritoneal route. Blood samples were collected at specific time points and at euthanasia from infected rabbits. Additionally, tissue samples from the heart, lungs, spleen, gastrointestinal tract, liver and kidneys were obtained at euthanasia. All experimentally infected rabbits displayed clinical signs of disease (fever, dehydration, weight loss and lethargy). Tissues collected at necropsy from the animals exhibited histopathological changes indicative of bacteremia. Non-typhoidal Salmonella bacteria were detected in the blood and tissue samples of infected rabbits by microbiological culture and real-time PCR assays. The development of this animal model of bacteremia could prove to be a useful tool for studying how non-typhoidal Salmonella infections disseminate and spread in humans.     

Farmer-Community Connections and the Future of Ecological Agriculture in California

While questions about the environmental sustainability of contemporary farming practices and the socioeconomic viability of rural communities are attracting increasing attention throughout the US, these two issues are rarely considered together. This paper explores the current and potential connections between these two aspects of sustainability, using data on community members’ and farmers’ views of agricultural issues in California’s Central Valley. These views were collected from a series of individual and group interviews with biologically oriented and conventional farmers as well as community stakeholders. Local marketing, farmland preservation, and perceptions of sustainable agriculture comprised the primary topics of discussion. The mixed results indicate that, while many farmers and community members have a strong interest in these topics, sustainable community development and the use of sustainable farming practices are seldom explicitly linked. On the other hand, many separate efforts around the Valley to increase local marketing and agritourism, improve public education about agriculture, and organize grassroots farmland preservation initiatives were documented. We conclude that linking these efforts more explicitly to sustainable agriculture and promoting more engagement between ecologically oriented farmers and their communities could engender more economic and political support for these farmers, helping them and their communities to achieve greater sustainability in the long run. Sonja Brodt is a former program evaluation specialist with the University of California Integrated Pest Management Program. Her current research focuses on extension and adoption of integrated pest management strategies by California growers and the impacts of pesticide safety training programs on farmworkers. Gail Feenstra is a food systems analyst at the University of California Sustainable Agriculture Research and Education Program (SAREP). She coordinates SAREP’s Community Development and Public Policy grants program and conducts outreach and education to academic and community-based groups to build their capacity and leadership skills for developing sustainable community food systems. Robin Kozloff is a social science researcher and consultant in agricultural and land use policy. Karen Klonsky is an extension specialist at the University of California at Davis in the Department of Agricultural and Resource Economics. Her research focuses on the economic viability of organic and sustainable farming systems as well as the evolution of the organic market. Laura Tourte is county director and farm advisor at the University of California Cooperative Extension in Santa Cruz County. Her research and extension activities currently focus on farm management and marketing for small-scale growers.     

Cryptosporidium and Giardia in locally harvested clams in Iqaluit,Nunavut

High prevalences of Cryptosporidium and Giardia were recently found in enteric illness patients in the Qikiqtaaluk region of Nunavut, Canada, with a foodborne, waterborne or animal source of parasites suspected. Clams (Mya truncata) are a commonly consumed, culturally important and nutritious country food in Iqaluit; however, shellfish may concentrate protozoan pathogens from contaminated waters. The goal of this work was to investigate clams as a potential source of Cryptosporidium and Giardia infections in residents in Iqaluit, Nunavut. The objectives were to estimate the prevalence and genetically characterize Cryptosporidium and Giardia in locally harvested clams. Clams (n = 404) were collected from Iqaluit harvesters in September 2016. Haemolymph (n = 328) and digestive gland (n = 390) samples were screened for Cryptosporidium and Giardia via PCR, and amplified products were further processed for sequence analyses for definitive confirmation. Giardia DNA was found in haemolymph from 2 clams, while Cryptosporidium was not detected. The two Giardia sequences were identified as zoonotic Giardia enterica assemblage B. The overall prevalence of Giardia in clams near Iqaluit was low (0.6%) compared with other studies in southern Canada and elsewhere. The presence of Giardia DNA in clams suggests human or animal faecal contamination of coastal habitat around Iqaluit in shellfish harvesting waters. Results from this study are intended to inform public health practice and planning in Inuit Nunangat.     

Climate-driven bedrock incision in an active mountain belt

Measurements of fluvial bedrock incision were made with submillimeter precision in the East Central Range of Taiwan, where long-term exhumation rates and precipitation-driven river discharge are independently known. They indicate that valley lowering is driven by relatively frequent flows of moderate intensity, abrasion by suspended sediment is an important fluvial wear process, and channel bed geometry and the presence of widely spaced planes of weakness in the rock mass influence erosion rate and style.