全文获取类型
收费全文 | 378篇 |
免费 | 19篇 |
专业分类
林业 | 35篇 |
农学 | 12篇 |
67篇 | |
综合类 | 20篇 |
农作物 | 14篇 |
水产渔业 | 37篇 |
畜牧兽医 | 189篇 |
园艺 | 4篇 |
植物保护 | 19篇 |
出版年
2023年 | 2篇 |
2022年 | 2篇 |
2021年 | 9篇 |
2020年 | 7篇 |
2019年 | 3篇 |
2018年 | 7篇 |
2017年 | 16篇 |
2016年 | 14篇 |
2015年 | 12篇 |
2014年 | 18篇 |
2013年 | 30篇 |
2012年 | 24篇 |
2011年 | 21篇 |
2010年 | 6篇 |
2009年 | 14篇 |
2008年 | 30篇 |
2007年 | 28篇 |
2006年 | 24篇 |
2005年 | 19篇 |
2004年 | 15篇 |
2003年 | 16篇 |
2002年 | 21篇 |
2001年 | 12篇 |
2000年 | 12篇 |
1999年 | 4篇 |
1998年 | 1篇 |
1997年 | 2篇 |
1996年 | 1篇 |
1994年 | 3篇 |
1993年 | 2篇 |
1992年 | 2篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1987年 | 2篇 |
1986年 | 2篇 |
1975年 | 2篇 |
1973年 | 1篇 |
1970年 | 2篇 |
1969年 | 1篇 |
1968年 | 2篇 |
1967年 | 3篇 |
1965年 | 1篇 |
1953年 | 1篇 |
排序方式: 共有397条查询结果,搜索用时 15 毫秒
21.
22.
23.
Kazuyuki?Mori Yu?Sakamoto Nobuhiro?Mukojima Seiji?Tamiya Takashi?Nakao Takashige?Ishii Kazuyoshi?HosakaEmail author 《Euphytica》2011,180(3):347-355
Multiplex PCR is practically a reasonable choice for molecular marker-assisted selection in potato breeding. We had developed
and were using a multiplex PCR method for selection of resistance genes to cyst nematode (H1), Potato virus X (Rx1) and late blight (R1 and R2). Since then, more reliable and tightly linked markers for H1 and R2, and a new marker for resistance to Potato virus Y (Ry
chc
) were developed. In this article, all these superior markers, including a positive marker to eliminate PCR-failed samples,
were incorporated into one multiplex PCR assay. Using the newly developed multiplex PCR technique, five plants potentially
harboring all five resistance genes were selected from 96 hybrid plants approximately 5 h after DNA extraction, which is a
third of the operation time compared with separate PCR reactions for each marker. 相似文献
24.
Yuko TAJIMA Kaori MAEDA Tadasu K. YAMADA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(1):45-51
One hundred and twenty
stranding events of Stejneger’s beaked whales were reported in Japan between 1999 and
2011. The purpose of this study is to introduce pathological data and to discuss probable
causes of death for 44 Stejneger’s beaked whales among them. The significant pathological
findings were the pulmonary edema, parasitic granulomatous nephritis, emaciation,
amyloidosis, suppurative bronchopneumonia and so on. The probable causes of death were
categorized as noninfectious in 43 of the cases, which included drowning, starvation and
secondary amyloidosis. One individual was diagnosed with septicemia, which was the only
example of an infectious disease. Because we could not always perform advanced analyses,
such as microbiology tests, biotoxin examinations or contaminant analyses, the finality of
our findings may be impaired. However, the present study has broad implications on the
causes of death of Stejneger’s beaked whales of the seas around Japan, which are valuable
for the future studies and for the detection of emerging diseases. 相似文献
25.
Yoshida H Momoi Y Taga N Ide K Yamazoe K Iwasaki T Kudo T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(6):663-669
Dendritic cells (DCs) are the most potent antigen-presenting cells that are expected to be therapeutic agents for tumor immunotherapy. In this study, we generated DCs of sufficient number for DC-based immunotherapy from peripheral blood mononuclear cells (PBMC) in dogs. PBMC were cultured in the presence of phytohemagglutinin (PHA). On day 6, large adherent cells with dendrite-like projections were seen, and the number of these large cells with projections increased on day 8. These cells were positive for esterase staining. They expressed MHC class II, CD11b, CD8 and weakly CD4 on their surface. They tended to make contact with lymphocytes under culture conditions. We obtained about 2-5 x 10(6) of DCs from 10 ml of peripheral blood. These DCs phagocytosed HEK-293 cells by overnight co-culturing. These cells generated from PBMC are possible canine DCs and are applicable to clinical trials of DC-based whole tumor cell immunotherapy in dogs. 相似文献
26.
27.
28.
29.
Khan MS Tanaka A Ide H Hoshinoo K Hanafusa Y Tagawa Y 《Veterinary microbiology》2005,107(3-4):179-192
The major outer membrane protein (MOMP) of Haemophilus somnus shows antigenic and molecular mass diversity that forms the basis of a preliminary grouping system for H. somnus strains. In this study, the gene encoding MOMP of H. somnus strain 8025 was cloned in three overlapping fragments by PCR techniques, and then sequenced. The gene consists of a 1164-bp open reading frame encoding a deduced 380-amino acid protein with a 19-amino acid signal sequence, giving a mature protein with a calculated molecular mass of 39,913 Da. Significant homology was found between MOMP and porin protein sequences of bacteria in Pasteurellaceae species. When expressed in Escherichia coli, the protein from the MOMP gene directed by the T7 promoter was identical in size (approximately 40 kDa) to native MOMP and reacted with MOMP-specific antibodies. Comparisons of the MOMP gene sequences from six unrelated strains of H. somnus to that of strain 8025 revealed that the genes of three MOMP type 1 strains were highly conserved with that of strain 8025 in length and sequence. However, two MOMP type 3c strains and one MOMP type 3a strain differed markedly from the MOMP of strain 8025 in their 3'-terminal halves. Their deduced MOMP amino acid sequences differed in sequence (3c, 80.5 and 82.7% identity; 3a, 62.4% identity) and in length (3c, 384 and 376; 3a, 316), indicating that the molecular differences are the basis of antigenicity and molecular mass differences of H. somnus MOMP. In the predicted MOMP secondary structure, the variable sequences primarily mapped to putative surface-exposed loops, and a variable and surface-exposed epitope of MOMP-specific antibody was identified in the seventh-largest loop. These findings are useful for understanding the structural and immunological characteristics of H. somnus. 相似文献
30.