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排序方式: 共有165条查询结果,搜索用时 15 毫秒
41.
Yuma SAHASHI Masaaki OSHIMA Junjiro YAMAGISHI Chieko MURAMATSU Kaori SHIMIZU Yasuo INOSHIMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(11):1730
Polymerase chain reaction (PCR) combined with restriction fragment length polymorphism (RFLP) is commonly used for genotyping bovine leukemia virus (BLV) in slaughterhouses. However, unclassified BLV genotypes have been sporadically reported. To assess the current status of BLV genetic characterization in cattle, PCR-RFLP was performed on blood samples of 170 cattle (84 Japanese Black, 60 Japanese Black x Holstein, and 26 Holstein) from 17 farms (5 prefectures) at a slaughterhouse in Aichi Prefecture in 2019. A total of 65 samples (38.2%) were BLV positive, and genotype 1 was the most predominant (56/65 samples), followed by genotypes 3 (6 samples) and 5 (1 sample), and two unclassified samples. No relationship between the genotypes and breeds was observed. Sequence and phylogenetic analyses demonstrated that unclassified BLV genotypes clustered with genotype 1 sequences were, therefore, not new genotypes. 相似文献
42.
Koichi Yoneyama Xiaonan Xie Kaori Yoneyama Takahito Nomura Ikuo Takahashi Tadao Asami Narumi Mori Kohki Akiyama Miyuki Kusajima Hideo Nakashita 《Pest management science》2019,75(9):2353-2359
Strigolactones (SLs) are carotenoid‐derived plant secondary metabolites that play important roles in various aspects of plant growth and development as plant hormones, and in rhizosphere communications with symbiotic microbes and also root parasitic weeds. Therefore, sophisticated regulation of the biosynthesis, perception and functions of SLs is expected to promote symbiosis of beneficial microbes including arbuscular mycorrhizal (AM) fungi and also to retard parasitism by devastating root parasitic weeds. We have developed SL mimics with different skeletons, SL biosynthesis inhibitors acting at different biosynthetic steps, SL perception inhibitors that covalently bind to the SL receptor D14, and SL function inhibitors that bind to the serine residue at the catalytic site. In greenhouse pot tests, TIS108, an azole‐type SL biosynthesis inhibitor effectively reduced numbers of attached root parasites Orobanche minor and Striga hermonthica without affecting their host plants; tomato and rice, respectively. AM colonization resulted in weak but distinctly enhanced plant resistance to pathogens. SL mimics can be used to promote AM symbiosis and to reduce the application rate of systemic‐acquired resistance inducers which are generally phytotoxic to horticultural crops. © 2019 Society of Chemical Industry 相似文献
43.
Akira Matsuda Yuki Shimizu Teppei Kanda Akihiro Ohnishi Noritaka Maeta Masahiro Miyabe Kaori Saeki Yoshiki Itoh 《Canadian journal of veterinary research》2021,85(3):236
Some immortalized lens epithelial cell lines have been established and are useful for molecular analysis. The establishment of additional cell lines must, however, enable a variety of in-vitro examinations. The objective of this study was to establish a new canine lens epithelial cell line by isolating CLC-1 cells from the lens tissue of a dog with cataracts. In CLC-1 cells, transforming growth factor beta (TGF-β) treatment significantly decreased gene expression of an epithelial marker and elevated that of mesenchymal markers; these characteristics are similar to those of a human lens epithelial cell line. Interestingly, CLC-1 cells exhibited lower expression of an epithelial marker and higher expression of mesenchymal markers than an anterior lens capsule. These results suggest that CLC-1 cells were derived from a cell population that was committed to epithelial-mesenchymal transition in cataract lens tissue. In conclusion, CLC-1 cells could be useful for analyzing molecular pathogenesis in canine cataracts. 相似文献
44.
Fukuzawa R Fukuzawa K Abe H Nagai T Kameyama K 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2004,33(1):39-42
Abstract: A male African pygmy hedgehog ( Atelerix albiventris ), estimated to be 3 years old, presented with exophthalmos and fixed abduction of the right eye. Radiographic examination revealed a retrobulbar tumor in the right orbital cavity. The mass was surgically resected but recurred 3 months later and the hedgehog died. There was no gross or microscopic evidence of salivary or lacrimal gland involvement of the tumor at surgery or at necropsy. The histopathologic, immunohistochemical, and ultrastructural findings were those of acinic cell carcinoma, the origin of which was unknown. This is the first known case of acinic cell carcinoma in an African hedgehog. 相似文献
45.
46.
Satoshi NOZAWA Hitomi ODA Ran AKIYAMA Kaori UEDA Kaori SAEKI Saori SHONO Natsuki MARUYAMA Atsuki MURATA Hiroyuki TAZAKI Akihiro MORI Yutaka MOMOTA Daigo AZAKAMI Toshinori SAKO Katsumi ISHIOKA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(8):1177-1182
47.
Ishii N Nakahigashi K Baba T Robert M Soga T Kanai A Hirasawa T Naba M Hirai K Hoque A Ho PY Kakazu Y Sugawara K Igarashi S Harada S Masuda T Sugiyama N Togashi T Hasegawa M Takai Y Yugi K Arakawa K Iwata N Toya Y Nakayama Y Nishioka T Shimizu K Mori H Tomita M 《Science (New York, N.Y.)》2007,316(5824):593-597
Analysis of cellular components at multiple levels of biological information can provide valuable functional insights. We performed multiple high-throughput measurements to study the response of Escherichia coli cells to genetic and environmental perturbations. Analysis of metabolic enzyme gene disruptants revealed unexpectedly small changes in messenger RNA and proteins for most disruptants. Overall, metabolite levels were also stable, reflecting the rerouting of fluxes in the metabolic network. In contrast, E. coli actively regulated enzyme levels to maintain a stable metabolic state in response to changes in growth rate. E. coli thus seems to use complementary strategies that result in a metabolic network robust against perturbations. 相似文献
48.
49.
Kaori MURATA Miho NAGASAWA Tatsushi ONAKA Ken-ichi TAKEYAMA Takefumi KIKUSUI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(3):478
The biological and psychological significance of oxytocin is increasingly recognized; however, reliable assays of oxytocin in biological samples have not been developed. We raised a new oxytocin polyclonal rabbit antibody against synthetic oxytocin. The affinity of antibodies to oxytocin was examined by a radio-immunoassay and compared with that of a previously validated antibody. One antibody showed affinity for oxytocin in the radio-immunoassay. We developed a solid-phase ELISA for oxytocin using this antibody and compared it with existing methods. The newly developed ELISA showed comparable results using urine samples but not using serum samples. These results indicate that the new ELISA is useful for urinary oxytocin; further modifications, such as different extraction methods, are needed for its application to serum oxytocin. 相似文献
50.