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141.
Sano J Oguma K Kano R Hasegawa A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(1):149-151
The canine Bcl-xL gene was cloned and sequenced. Canine Bcl-xL cDNA clone was 1252 bp in length, and encoded 233 deduced amino acides. The predicted canine Bcl-xL amino acid sequence shared 99.6%, 97.0%, 97.9%, 98.7% and 98.3% homology with that of human, mouse, rat, sheep and pig Bcl-xL, respectively. RT-PCR analysis revealed that canine Bcl-xL mRNA was constitutively expressed in CL-1 (canine lymphoma) and GL-1 (canine B cell leukemia) cell lines. 相似文献
142.
Kano R Hirai A Muramatsu M Watari T Hasegawa A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(2):267-270
For the direct detection of dermatophytes in skin scrapings and hairs from animals, a primer pair specific to the chitin synthase 1 (CHS1) gene of dermatophytes was constructed. By PCR analysis with the primer pair, dermatophyte DNA could be diagnosed directly and rapidly in clinical skin samples. 相似文献
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Kozaki M Nakamura Y Iguchi M Kano R Watanabe S Fujiwara K Hasegawa A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(1):1-4
The expression of cytokeratins and involucrin was analyzed to identify the skin cells which compose the epidermis of dogs. The distribution of cytokeratins and involucrin in normal dog skin was immunohistochemically examined with 27 commercial monoclonal antibodies for human use. Antibodies, No.4. OV-TL12/13, 35betaH11, 4.1.18, CAM5.2, NCL5D3, Ks.13.1, Ks.18.04, Ks.19.1, 170.2.]4 and Ks.20.8 stained hair follicles and/or the sweat gland duct, but not the epidermis. Antibodies, 34betaB4, AE3, 34betaE12. LP34, RCK102, MNF116, AE1, KLI, DE-K10 and DE-K13 reacted with every layer of the epidermis, hair follicles and the sweat gland duct. These results were similar to those reported in the human skin. No positive staining, however, could be detected in the epidermis, hair follicles and the sweat gland duct with commercial antibodies, 6B10, Ks.7.18, Mu146-uc, E3, RCK108 and involucrin. Therefore, immunohistochemical investigation with these commercial antibodies developed for human skin examination might be available for investigating the origin of skin tumors in dogs. 相似文献
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148.
Continuous plasma outflows from the edge of a solar active region as a possible source of solar wind
Sakao T Kano R Narukage N Kotoku J Bando T Deluca EE Lundquist LL Tsuneta S Harra LK Katsukawa Y Kubo M Hara H Matsuzaki K Shimojo M Bookbinder JA Golub L Korreck KE Su Y Shibasaki K Shimizu T Nakatani I 《Science (New York, N.Y.)》2007,318(5856):1585-1588
The Sun continuously expels a huge amount of ionized material into interplanetary space as the solar wind. Despite its influence on the heliospheric environment, the origin of the solar wind has yet to be well identified. In this paper, we report Hinode X-ray Telescope observations of a solar active region. At the edge of the active region, located adjacent to a coronal hole, a pattern of continuous outflow of soft-x-ray-emitting plasmas was identified emanating along apparently open magnetic field lines and into the upper corona. Estimates of temperature and density for the outflowing plasmas suggest a mass loss rate that amounts to approximately 1/4 of the total mass loss rate of the solar wind. These outflows may be indicative of one of the solar wind sources at the Sun. 相似文献
149.
Sho SATO Toshikiyo TAKAHASHI Hiroshi NISHINOMIYA Makiko KATOH Ryu ITOH Masaki YOKOO Mari YOKOO Momoe IHA Yuki MORI Kano KASUGA Ikuo KOJIMA Masayuki KOBAYASHI 《Animal Science Journal》2012,83(3):260-262
Fibroblast growth factor 4 (FGF4) is considered as a crucial gene for the proper development of bovine embryos. However, the complete nucleotide sequences of the structural genes encoding FGF4 in identified breeds are still unknown. In the present study, direct sequencing of PCR products derived from genomic DNA samples obtained from three Japanese Black, two Japanese Shorthorn and three Holstein cattle, revealed that the nucleotide sequences of the structural gene encoding FGF4 matched completely among these eight cattle. On the other hand, differences in the nucleotide sequences, leading to substitutions, insertions or deletions of amino acid residues were detected when compared with the already reported sequence from unidentified breeds. We cannot rule out a possibility that the structural gene elucidated in the present study is widely distributed in cattle. To the best of our knowledge, this is the first determination of the complete nucleotide sequence of the structural gene encoding bovine FGF4 in identified breeds. 相似文献
150.
Shota Kono Tomohiko Kazama Koichiro Kano Kayoko Harada Masami Uechi Taro Matsumoto 《Veterinary journal (London, England : 1997)》2014,199(1):88-96
It has been reported that mature adipocyte-derived dedifferentiated fat (DFAT) cells show multilineage differentiation potential similar to that observed in mesenchymal stem cells. Since DFAT cells can be prepared from a small quantity of adipose tissue, they could facilitate cell-based therapies in small companion animals such as cats. The present study examined whether multipotent DFAT cells can be generated from feline adipose tissue, and the properties of DFAT cells were compared with those of adipose-derived stem cells (ASCs). DFAT cells and ASCs were prepared from the floating mature adipocyte fraction and the stromal vascular fraction, respectively, of collagenase-digested feline omental adipose tissue. Both cell types were evaluated for growth kinetics, colony-forming unit fibroblast (CFU-F) frequency, immunophenotypic properties, and multilineage differentiation potential.DFAT cells and ASCs could be generated from approximately 1 g of adipose tissue and were grown and subcultured on laminin-coated dishes. The frequency of CFU-Fs in DFAT cells (35.8%) was significantly higher than that in ASCs (20.8%) at passage 1 (P1). DFAT cells and ASCs displayed similar immunophenotypes (CD44+, CD90+, CD105+, CD14?, CD34? and CD45?). Alpha-smooth muscle actin-positive cells were readily detected in ASCs (15.2 ± 7.2%) but were rare in DFAT cells (2.2 ± 3.2%) at P1. Both cell types exhibited adipogenic, osteogenic, chondrogenic, and smooth muscle cell differentiation potential in vitro. In conclusion, feline DFAT cells exhibited similar properties to ASCs but displayed higher CFU-F frequency and greater homogeneity. DFAT cells, like ASCs, may be an attractive source for cell-based therapies in cats. 相似文献