全文获取类型
收费全文 | 167篇 |
免费 | 14篇 |
专业分类
林业 | 18篇 |
农学 | 3篇 |
24篇 | |
综合类 | 12篇 |
农作物 | 4篇 |
水产渔业 | 17篇 |
畜牧兽医 | 93篇 |
园艺 | 4篇 |
植物保护 | 6篇 |
出版年
2023年 | 1篇 |
2021年 | 4篇 |
2020年 | 3篇 |
2019年 | 1篇 |
2018年 | 4篇 |
2017年 | 5篇 |
2016年 | 7篇 |
2015年 | 10篇 |
2014年 | 6篇 |
2013年 | 18篇 |
2012年 | 11篇 |
2011年 | 12篇 |
2010年 | 5篇 |
2009年 | 5篇 |
2008年 | 11篇 |
2007年 | 12篇 |
2006年 | 8篇 |
2005年 | 8篇 |
2004年 | 12篇 |
2003年 | 9篇 |
2002年 | 6篇 |
2001年 | 5篇 |
2000年 | 4篇 |
1999年 | 2篇 |
1998年 | 1篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1993年 | 1篇 |
1991年 | 1篇 |
1988年 | 2篇 |
1987年 | 2篇 |
1985年 | 2篇 |
1978年 | 1篇 |
排序方式: 共有181条查询结果,搜索用时 15 毫秒
41.
In the previous paper1) the authors proved that the ferric iron reduction in submerged paddy soils is entirely or mostly effected by the activities of microorganisms in the soil. Prior to the study on the ferric iron reducing microorganisms isolated from the soil, the present paper reports the research for the microbial mechanism of iron reduction. working with the soil itself. 相似文献
42.
Shin-ichi Yamasaki 《Soil Science and Plant Nutrition》2013,59(2):305-308
Recently conventional chemical analyses were mostly replaced by instrumental analysis. Although results from both methods were examined in details after the samples had been solubilized or extracted by appropriate techniques, the solubilization method was considerably altered so as to be convenient for the following analysis. In atomic absorption spectrophotometry (AAS), for example, fusion technique was mostly inadequate because of the higher salts concentration in the obtained solution. The use of H2SO4 must also be avoided in acid digestion for SO4 2- precipitates with Sr2+ or La3+ which must be added to eliminate interferences in the succeeding procedure. Therefore, it is essential to consider the whole scheme including sample treatments to evaluate the suitability of instrumental analysis. In this experiment, the suitability of the methods which have been employed in our laboratory was examined by analyzing 9 rock standards. 相似文献
43.
Fumio NAMIKI Kanji SHIMIZU Kyoko SATOH Tetsuo HIRABAYASHI Kazufumi NISHI Tsuruo KAYAMURA Takashi TSUGE 《Journal of General Plant Pathology》2000,66(1):12-17
In 1994, Fusarium wilt of melon cultivars which are resistant to races 0 and 2 of Fusarium oxysporum f. sp. melonis was observed in southern area of the Lake Biwa region, Shiga prefecture. In commercial fields, mature plants of cv. Amus
which were grafted onto cv. Enken Daigi 2, and of cv. FR Amus showed yellowing, wilting and finally death before harvesting
of fruits. Diseased plants had vascular and root discolorations, and their stem sections yielded typical colonies of F. oxysporum. When the Shiga strains were tested for their pathogenicity to 12 species of cucurbits, they caused wilts only on melon.
Using race differential cultivars of melon, the Shiga strains were classified as race 1 of F. oxysporum f. sp. melonis, which has not been reported in Japan. To further characterize their pathogenicity, the strains were used to inoculate 46
additional cultivars of melon, oriental melon and oriental pickling melon. All the race 1 strains were pathogenic to the cultivars
tested, and their host range was apparently different from those of strains belonging to other races (races 0, 2 and 1,2y).
DNA fingerprinting with a repetitive DNA sequence, FOLR3, differentiated race 1 strains from strains of races 0 and 2, but
not from race 1,2y strains.
Received 2 July 1999/ Accepted in revised form 30 September 1999 相似文献
44.
Yuichiro Sato Kinjiro Morimoto Takanori Kubo Takemasa Sakaguchi Akira Nishizono Makoto Hirayama Kanji Hori 《Marine drugs》2015,13(6):3454-3465
Lectin sensitivity of the recent pandemic influenza A virus (H1N1-2009) was screened for 12 lectins with various carbohydrate specificity by a neutral red dye uptake assay with MDCK cells. Among them, a high mannose (HM)-binding anti-HIV lectin, ESA-2 from the red alga Eucheuma serra, showed the highest inhibition against infection with an EC50 of 12.4 nM. Moreover, ESA-2 exhibited a wide range of antiviral spectrum against various influenza strains with EC50s of pico molar to low nanomolar levels. Besides ESA-2, HM-binding plant lectin ConA, fucose-binding lectins such as fungal AOL from Aspergillus oryzae and AAL from Aleuria aurantia were active against H1N1-2009, but the potency of inhibition was of less magnitude compared with ESA-2. Direct interaction between ESA-2 and a viral envelope glycoprotein, hemagglutinin (HA), was demonstrated by ELISA assay. This interaction was effectively suppressed by glycoproteins bearing HM-glycans, indicating that ESA-2 binds to the HA of influenza virus through HM-glycans. Upon treatment with ESA-2, no viral antigens were detected in the host cells, indicating that ESA-2 inhibited the initial steps of virus entry into the cells. ESA-2 would thus be useful as a novel microbicide to prevent penetration of viruses such as HIV and influenza viruses to the host cells. 相似文献
45.
Effect of sodium acetate on the adhesion to porcine gastric mucin in a Lactococcus lactis strain grown on fructose
下载免费PDF全文
![点击此处可从《Animal Science Journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Hiromi Kimoto‐Nira Naoko Moriya Seishi Yamasaki Akio Takenaka Chise Suzuki 《Animal Science Journal》2016,87(6):802-808
The association of lactic acid bacteria with mucosal surfaces plays important roles in the beneficial effects of these bacteria on human health, such as colonization of the gastrointestinal tract for pathogen antagonism. Previously, we found that the adhesion of Lactococcus lactis 7‐1 to porcine gastric mucin was higher with fructose than with lactose, galactose or xylose as the carbon source. In this study, we examined the effect of growth conditions on the adhesion of strain 7‐1 grown on fructose. Medium components affect the adhesion: the adhesion of strain 7‐1 grown with sodium acetate was higher than that without it. The enhancement of adhesion by sodium acetate was not observed under aerobic conditions. Cellular properties grown with or without sodium acetate were characterized: strain 7‐1 grown with sodium acetate had similar sugar contents, and different fatty acid composition to those grown without it. Strain 7‐1 grown with sodium acetate showed significantly lower cell yield and significantly higher hydrophobicity than those grown without it, which is associated with higher adhesion. Fructose and sodium acetate are frequently used in the food industry; this study may reveal a simple way to enhance the adhesion of lactic acid bacteria by growing them with these substances. 相似文献
46.
Hiroi M Yamazaki F Harada T Takahashi N Iida N Noda Y Yagi M Nishio T Kanda T Kawamori F Sugiyama K Masuda T Hara-Kudo Y Ohashi N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(2):189-195
To evaluate the diversity of extended-spectrum β-lactamases (ESBL) genes among food-producing animals, 48 isolates of ESBL-producing Escherichia coli isolates were obtained from rectal samples of broilers, layers, beef cattle and pigs, at the slaughterhouse level. ESBL-carrying E. coli were isolated from 60.0% of individual broiler rectal samples, 5.9% of layers, 12.5% of beef cattle and 3% of pigs. One ESBL-producing Klebsiella pneumoniae was isolated from a broiler. The ESBL-positive E. coli isolates from broilers harbored various ESBL genes: bla (SHV-12), bla(CTX-M-2), bla(CTX-M-14), bla(CTX-M-15) and bla(CTX-M-44). The plasmid DNAs were analyzed by restriction patterns. Homogeneous band patterns were yielded in those of K. pneumoniae and E. coli isolates harboring the bla(CTX-M-2) gene from different farms. No genetic relation between the 2 CTX-M-14 ESBL-producing strains was found by pulsed-field gel electrophoresis, although 2 plasmids in these strains, obtained from different broiler farms, were similar to each other. This study provides evidence that the proliferation of CTX-M-producing E. coli is due to the growth of indigenous CTX-M-producing strains and the possible emergence of strains that acquired CTX-M genes by horizontal transfer in different broiler farms. CTX-M-producing coliforms in broilers should be controlled due to the critical importance of cephalosporins and the zoonotic potential of ESBL-producing bacteria. 相似文献
47.
K Yamasaki T Taga Y Hirata H Yawata Y Kawanishi B Seed T Taniguchi T Hirano T Kishimoto 《Science (New York, N.Y.)》1988,241(4867):825-828
Interleukin-6 (IL-6/BSF-2/IFN beta 2) is a multifunctional cytokine that regulates the growth and differentiation of various tissues, and is known particularly for its role in the immune response and acute phase reactions. A complementary DNA encoding the human IL-6 receptor (IL-6-R) has now been isolated. The IL-6-R consists of 468 amino acids, including a signal peptide of approximately 19 amino acids and a domain of approximately 90 amino acids that is similar to a domain in the immunoglobulin (Ig) superfamily. The cytoplasmic domain of approximately 82 amino acids lacks a tyrosine/kinase domain, unlike other growth factor receptors. 相似文献
48.
Suzuki N Suzuki S Millar DG Unno M Hara H Calzascia T Yamasaki S Yokosuka T Chen NJ Elford AR Suzuki J Takeuchi A Mirtsos C Bouchard D Ohashi PS Yeh WC Saito T 《Science (New York, N.Y.)》2006,311(5769):1927-1932
IRAK-4 is a protein kinase that is pivotal in mediating signals for innate immune responses. Here, we report that IRAK-4 signaling is also essential for eliciting adaptive immune responses. Thus, in the absence of IRAK-4, in vivo T cell responses were significantly impaired. Upon T cell receptor stimulation, IRAK-4 is recruited to T cell lipid rafts, where it induces downstream signals, including protein kinase C activation through the association with Zap70. This signaling pathway was found to be required for optimal activation of nuclear factor kappaB. Our findings suggest that T cells use this critical regulator of innate immunity for the development of acquired immunity, suggesting that IRAK-4 may be involved in direct signal cross talk between the two systems. 相似文献
49.
The ambrosia beetle Platypus quercivorus is a vector for the fungus that causes Japanese oak wilt, and susceptibility to infestation by P. quercivorus varies by tree species. We postulated that P. quercivorus discriminates among host tree species differing in susceptibility to attack. To test this postulate, we counted the number
of flying male beetles (NFM), the number of holes bored by male beetles (NH), and the number of flying female beetles (NFF)
per unit area of bark surface in three fagaceous tree species: Quercus crispula (with high susceptibility to infestation) and Q. serrata and Castanea crenata (both with low susceptibility). NFM and NH were used to calculate the proportion of male beetles that bored holes out of
those that flew to the tree (PBM). We used generalized additive models to predict NFM, NFF, and PBM. The locations of trees,
expressed as x and y coordinates, numbers of weeks after the first male beetle’s flying (WEEK), diameters of trees 130 cm above ground (DBH),
and tree species (SP) were incorporated into the models as candidate explanatory variables. The best-fit models for NFM and
NFF included WEEK and DBH and the effect of location; SP was not included in the models. For PBM, the best-fit model included
WEEK, DBH, and SP. The results indicate that male P. quercivorus prefer Q. crispula to Q. serrata and C. crenata and that selection is made before boring holes on trees, but that P. quercivorus do not discriminate among host species when they fly to trees. 相似文献
50.
Doi J Hirota J Morita A Fukushima K Kamijyo H Ohta H Yamasaki M Takahashi T Katakura K Oku Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(4):413-417
Tritrichomonas suis (=T. foetus) has recently been reported to be a causative agent of chronic large-bowel diarrhea in cats. While the disease was previously attributed to Pentatrichomonas hominis, the etiologic agent for feline trichomonal diarrhea was identified as T. suis. Although feline trichomonosis due to T. suis has been reported at prevalences ranging from 14 to 31% in Europe and the U.S., no reports of the pathogen have been published to date in Japan. In 2008, however, we encountered a case of feline trichomonosis at the Veterinary Teaching Hospital of Hokkaido University. The parasite was identified as T. suis by nested PCR amplification of partial internal transcribed spacer region 1 and 5.8S ribosomal RNA gene sequences with T. suis-specific primers and DNA sequencing of the amplified products. We then conducted surveys for feline trichomonosis in three different animal hospitals using either cultivation and/or PCR-based assays. The results revealed that 13 of 147 samples (8.8%) were positive for T. suis, and that 5 of the 13 infected cats, which ranged between 1 month and 7.5 years-old, showed chronic diarrhea. Seven of the infected cats were purebred and 6 were mixed breed. These findings suggested that feline trichomonosis is prevalent in Japan, and that T. suis may play a role as a causative agent of feline chronic diarrhea. 相似文献