Genetic manipulation for enhancing calcium content in potato tuber

Increased calcium (Ca) in potatoes may increase the production rate by enhancing tuber quality and storability. Additionally, increased Ca levels in important agricultural crops may help ameliorate the incidence of osteoporosis. However, the capacity to alter Ca levels in potato tubers through genetic manipulations has not been previously addressed. Here we demonstrate that potato tubers expressing the Arabidopsis H+/Ca2+ transporter sCAX1 (N-terminal autoinhibitory domain truncated version of CAtion eXchanger 1) contain up to 3-fold more Ca than wild-type tubers. The increased Ca appears to be distributed throughout the tuber. The sCAX1-expressing potatoes have normally undergone the tuber/plant/tuber cycle for three generations; the trait appeared stable through successive generations. The expression of sCAX1 does not appear to alter potato growth and development. Furthermore, increased Ca levels in sCAX1-expressing tubers do not appear to alter tuber morphology or yield. Given the preponderance of potato consumption worldwide, these transgenic plants may be a means of marginally increasing Ca intake levels in the population. To our knowledge, this study represents the first attempts to use biotechnology to increase the Ca content of potatoes.     

黄土区不同下垫面农田降雨入渗及产流关系的数值模拟

在野外试验的基础上，建立了二维农田降雨入渗及产流关系的数学模型，并用中国科学院长武县王东沟农业生态站不同下垫面径流小区实测土壤含水率及产流资料对该模型进行验证。结果显示：该模型对降雨过程中土壤入渗、产流以及雨后蒸发条件下土壤水分的模拟都有很高的精度，可以用于黄土区降雨入渗及产流关系的模拟。     

沟灌夏玉米棵间土壤蒸发规律的试验研究

棵间土壤蒸发是农田土壤耗水的重要组成部分。该文采用两种规格的微型棵间蒸发皿(Micro-Lysimeter)分别测定沟灌夏玉米田沟、垄土面蒸发量,并对沟灌条件下夏玉米棵间土壤蒸发与作物蒸腾变化规律进行了试验研究,分析了相对棵间土壤蒸发强度与土壤含水率的关系以及棵间土壤蒸发强度与作物叶面积指数的关系。结果表明,沟灌条件下夏玉米棵间土壤蒸发量占全生育总耗水量的33.06%～34.35%,棵间土壤相对蒸发强度与表层土壤含水率和作物叶面积指数之间均呈现良好的指数函数关系,灌溉或降雨后2～3 d内土壤蒸发强度较大,受大气蒸发力影响明显。因此,在不影响作物蒸腾的条件下减少表层土壤的湿润面积和湿润次数是减少棵间土壤蒸发、提高作物水分利用效率的主要技术途径与措施。     

河北省海洋生物资源开发现状及可持续利用对策

介绍了位于环渤海经济圈中心地带的河北省海岸带和海洋经济概况,分析了河北省海洋生物资源的开发利用现状,并从政府管理、生态环境修复、加大海洋科技与教育投入、海洋生物资源摸底调查、海洋渔业产业结构的调整和海洋药用生物资源的开发等六个方面提出了保护和合理利用河北省海洋生物资源的对策,可为海洋生物资源的可持续利用提供参考依据。     

氯酸钾对龙眼催花的效应及其机理研究综述

综述氯酸钾对龙眼催花的效应及其机理,概述应用氯酸钾促使龙眼开花应注意及存在的问题。     

基于组织培养技术的姜科植物快繁体系的研究进展

为解决姜科植物繁殖系数低、种性退化等问题,扩大姜科植物的繁殖规模,提高品种自身抗逆性和增产潜力,有效建立姜科植物的离体快繁体系,总结了基于植物组织培养技术的部分姜科植物离体快繁体系构建过程中外植体的选择与消毒处理、丛生芽和愈伤组织的诱导、增殖与分化和生根培养条件,阐述了姜科植物离体快繁体系建立过程中存在的问题及解决方案,同时对植物组织培养在姜科植物中的应用进行了展望,以期为姜科植物产业的健康、快速发展提供参考。     

Evaluation of Caspase-3 and Ki-67 expression in squamous cell hyperplasia of the stomach induced by Platycodi radix water extract in Sprague–Dawley rats

Platycodi radix is widely used in traditional herbal medicine for the treatment of bronchitis, asthma, pulmonary tuberculosis, hypertension, hyperlipidemia, and diabetes. This study aimed to investigate cell proliferation (Ki-67) and apoptosis (Caspase-3) potential in squamous cell hyperplasia of the stomach induced by a Platycodi radix water extract in a subchronic toxicity study. One hundred formalin-fixed, paraffin-embedded stomach tissues of rats treated with Platycodi radix at doses of 0, 500, 1,000, and 3,000 mg/kg body weight/day were used for the analysis. They were conventionally stained using hematoxylin and eosin (H&E) and immunohistochemically (IHC) stained using caspase-3 and Ki-67 antibodies. The incidence of squamous cell hyperplasia was significantly increased in the 3,000 mg/kg b.w./day treatment group in both sexes (p<0.01). However, the hyperplastic change was completely repaired after 4 weeks of recovery period. Ki-67 expression was similar in all groups, with no statistically significant differences among the groups. Caspase-3 expression was significantly increased in both sexes in the 3,000 mg/kg b.w./day treatment group (p<0.01), compared with the vehicle control groups, and then reduced to normal levels in the recovery groups in both sexes. In conclusion, this study showed that squamous cell hyperplasia induced by the Platycodi radix water extract in the limiting ridge of the stomach is not considered to be abnormal proliferative change; as a result, squamous cell hyperplasia is considered to be a non-adverse effect when induced by the oral administration of the Platycodi radix water extract once daily for 13 weeks in rats.     

铜川市耀州区农户肉羊农牧生态系统能量流动规律的研究

运用生态学的系统分析方法,以铜川市耀州区金圣生态养羊示范基地农户肉羊农牧生态系统为研究对象,对系统的能量流动规律、种植业与养殖业的生态效率进行分析.分析表明三种规模养殖农户种植亚系统能量产投比与结构调整前相比分别提高了39.40%、39.50%和30.40%,向养殖亚系统输入的总能量占养殖亚系统需能总量的96.61%,88.45%和80.55%;养殖亚系统的能量转化效率分别达到7.35%、8.00%和8.39%,输入到种植亚系统的粪尿能水平分别是调整前粪尿能输入水平(37.64×103 MJ/hm2)的1.57倍、1.84倍和2.07倍.整个系统的经济投入与产出比分别达到1.73、1.83和1.98,三种规模农户的纯收益比调整前分别增加1132.5元、2392.8元和3913.5元.表明农户肉羊生产系统是一个高投入、高产出的农牧生态系统,有较好的生态效益和经济效益.     

红莲型CMS水稻线粒体K+ATP通道特性的初步研究

以水稻红莲型细胞质雄性不育(HL-CMS)不育系粤泰A(YTA)和保持系粤泰B(YTB)黄化苗线粒体为材料,研究了YTA和YTB离体线粒体KA TP通道对其诱导调节剂KCl、ATP、ADP和GTP等的响应特性。结果表明,KA TP通道诱导剂KCl,能明显诱导YTA和YTB线粒体膨胀,但YTA的膨胀程度较YTB的明显;KA TP通道的内源性抑制剂ATP对YTA和YTB线粒体膨胀起显著抑制作用;KA TP通道的内源性激动剂ADP和GTP引起的不育系YTA离体线粒体短暂收缩及之后的再膨胀程度均较YTB的明显;此外,比较YTA和YTB离体线粒体在Rh123一起孵育10 min后,KCl和解偶联剂FCCP处理引起的离体线粒体膜电位(Δψm)下降过程,发现前者的线粒体Δψm较后者的易于过早崩解。对水稻HL-CMS不育系YTA和可育的保持系YTB线粒体KA TP通道特性的比较研究表明,水稻HL-CMS不育系YTA线粒体KA TP通道对其诱导调节剂KCl、ATP、ADP和GTP等的响应较YTB的更敏感。     

人参皂苷Rg3(R),Rg3(S),Rg5/Rk1对乙醇致小鼠记忆阻碍改善作用的影响

采用被动回避穿越法研究了人参皂苷Rg3(R),Rg3(S)和Rg5/Rk1(1∶1)对乙醇致小鼠记忆阻碍改善作用的影响。结果表明:灌服人参皂苷Rg3(R),Rg3(S)和Rg5/Rk110 mg/kg后,显著延长了小鼠避暗的潜伏期,尤其以人参皂苷Rg5/Rk1作用最为显著,Rg5/Rk1处理组的潜伏期是对照组的2.97倍,Rg3(R),Rg3(S)分别为对照组的2.35和2.53倍,差异极显著(P<0.01)。说明这些化合物具有改善乙醇致小鼠的记忆障碍的能力。